Restriction map for
pF1KA0595
(Linear) (Six-base) MAPSORT of: KIAA0595.seq Check: 302 from: 1 to: 4992
KIAA0595.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 1344 2526 2638 4992
Size: 141 1203 1182 112 2354
Fragments arranged by size:
2354 1203 1182 141 112
AccI GT'mk_AC
Cuts at: 0 1459 1490 1730 2983 4992
Size: 1459 31 240 1253 2009
Fragments arranged by size:
2009 1459 1253 240 31
Acc65I G'GTAC_C
Cuts at: 0 2212 4992
Size: 2212 2780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 973 2192 3281 3482 3808 3815 4992
Size: 747 226 1219 1089 201 326 7 1177
Fragments arranged by size:
1219 1177 1089 747 326 226 201 7
AflIII A'CryG_T
Cuts at: 0 2926 4992
Size: 2926 2066
AgeI A'CCGG_T
Cuts at: 0 3644 4992
Size: 3644 1348
AleI CACnn'nnGTG
Cuts at: 0 4727 4992
Size: 4727 265
AlwNI CAG_nnn'CTG
Cuts at: 0 156 418 889 1198 3181 4921 4992
Size: 156 262 471 309 1983 1740 71
Fragments arranged by size:
1983 1740 471 309 262 156 71
ApaI G_GGCC'C
Cuts at: 0 55 211 563 2942 2956 4015 4992
Size: 55 156 352 2379 14 1059 977
Fragments arranged by size:
2379 1059 977 352 156 55 14
ApaLI G'TGCA_C
Cuts at: 0 4713 4992
Size: 4713 279
ApoI r'AATT_y
Cuts at: 0 1599 4933 4992
Size: 1599 3334 59
Fragments arranged by size:
3334 1599 59
AscI GG'CGCG_CC
Cuts at: 0 7 4992
Size: 7 4985
AvaI C'yCGr_G
Cuts at: 0 42 473 1562 1574 4049 4992
Size: 42 431 1089 12 2475 943
Fragments arranged by size:
2475 1089 943 431 42 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1460 1493 4992
Size: 1460 33 3499
Fragments arranged by size:
3499 1460 33
BanI G'GyrC_C
Cuts at: 0 1311 2212 2538 2826 2851 2889 4992
Size: 1311 901 326 288 25 38 2103
Fragments arranged by size:
2103 1311 901 326 288 38 25
BanII G_rGCy'C
Cuts at: 0 55 81 211 484 563 1368 1575 2942
Size: 55 26 130 273 79 805 207 1367
Cuts at: 2942 2956 3141 3683 3877 3955 4015 4992
Size: 14 185 542 194 78 60 977
Fragments arranged by size:
1367 977 805 542 273 207 194 185
130 79 78 60 55 26 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 2416 2695 4334 4463 4626 4910 4992
Size: 2416 279 1639 129 163 284 82
Fragments arranged by size:
2416 1639 284 279 163 129 82
BbvCI CC'TCA_GC
Cuts at: 0 1435 4095 4185 4772 4992
Size: 1435 2660 90 587 220
Fragments arranged by size:
2660 1435 587 220 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4205 4239 4992
Size: 4205 34 753
Fragments arranged by size:
4205 753 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 820 4992
Size: 820 4172
BclI T'GATC_A
Cuts at: 0 1892 4062 4992
Size: 1892 2170 930
Fragments arranged by size:
2170 1892 930
BfrBI ATG'CAT
Cuts at: 0 828 4037 4992
Size: 828 3209 955
Fragments arranged by size:
3209 955 828
BglI GCCn_nnn'nGGC
Cuts at: 0 2766 3673 4268 4992
Size: 2766 907 595 724
Fragments arranged by size:
2766 907 724 595
BglII A'GATC_T
Cuts at: 0 398 4992
Size: 398 4594
BlpI GC'TnA_GC
Cuts at: 0 1880 4992
Size: 1880 3112
Bme1580I G_kGCm'C
Cuts at: 0 55 211 563 1208 1316 2609 2831 2892
Size: 55 156 352 645 108 1293 222 61
Cuts at: 2892 2942 2956 3061 4015 4717 4992
Size: 50 14 105 954 702 275
Fragments arranged by size:
1293 954 702 645 352 275 222 156
108 105 61 55 50 14
BmgBI CAC'GTC
Cuts at: 0 2083 4992
Size: 2083 2909
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 557 565 1078 1894 2041 2336 2663
Size: 538 19 8 513 816 147 295 327
Cuts at: 2663 2899 4992
Size: 236 2093
Fragments arranged by size:
2093 816 538 513 327 295 236 147
19 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 501 921 3969 4992
Size: 501 420 3048 1023
Fragments arranged by size:
3048 1023 501 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 693 1057 1096 1201 1528 2021 2993
Size: 376 317 364 39 105 327 493 972
Cuts at: 2993 3047 3547 3674 4992
Size: 54 500 127 1318
Fragments arranged by size:
1318 972 500 493 376 364 327 317
127 105 54 39
Bpu10I CC'TnA_GC
Cuts at: 0 1435 2492 4095 4185 4772 4992
Size: 1435 1057 1603 90 587 220
Fragments arranged by size:
1603 1435 1057 587 220 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 2464 4227 4992
Size: 997 1467 1763 765
Fragments arranged by size:
1763 1467 997 765
BsaI GGTCTCn'nnnn_
Cuts at: 0 640 669 1107 2562 3301 3310 4992
Size: 640 29 438 1455 739 9 1682
Fragments arranged by size:
1682 1455 739 640 438 29 9
BsaAI yAC'GTr
Cuts at: 0 3448 4992
Size: 3448 1544
BsaBI GATnn'nnATC
Cuts at: 0 4515 4992
Size: 4515 477
BsaWI w'CCGG_w
Cuts at: 0 60 3644 3899 4304 4992
Size: 60 3584 255 405 688
Fragments arranged by size:
3584 688 405 255 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2108 2125 2138 2155 4155 4185 4992
Size: 2108 17 13 17 2000 30 807
Fragments arranged by size:
2108 2000 807 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 256 474 760 763 859 897 1270
Size: 181 75 218 286 3 96 38 373
Cuts at: 1270 2116 3095 3131 3292 3557 4723 4789 4887
Size: 846 979 36 161 265 1166 66 98
Cuts at: 4887 4992
Size: 105
Fragments arranged by size:
1166 979 846 373 286 265 218 181
161 105 98 96 75 66 38 36
3
BseYI C'CCAG_C
Cuts at: 0 204 270 698 719 1986 2120 2400 2470
Size: 204 66 428 21 1267 134 280 70
Cuts at: 2470 3408 4014 4914 4992
Size: 938 606 900 78
Fragments arranged by size:
1267 938 900 606 428 280 204 134
78 70 66 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 2108 2605 4992
Size: 475 1633 497 2387
Fragments arranged by size:
2387 1633 497 475
BsiHKAI G_wGCw'C
Cuts at: 0 1127 1368 3141 3382 4717 4992
Size: 1127 241 1773 241 1335 275
Fragments arranged by size:
1773 1335 1127 275 241 241
BsmI GAATG_Cn'
Cuts at: 0 443 4992
Size: 443 4549
BsmBI CGTCTCn'nnnn_
Cuts at: 0 17 4992
Size: 17 4975
Bsp1286I G_dGCh'C
Cuts at: 0 55 81 211 484 563 1127 1208 1316
Size: 55 26 130 273 79 564 81 108
Cuts at: 1316 1368 1575 2609 2831 2892 2942 2956 3061
Size: 52 207 1034 222 61 50 14 105
Cuts at: 3061 3141 3382 3683 3877 3955 4015 4717 4992
Size: 80 241 301 194 78 60 702 275
Fragments arranged by size:
1034 702 564 301 275 273 241 222
207 194 130 108 105 81 80 79
78 61 60 55 52 50 26 14
BspEI T'CCGG_A
Cuts at: 0 60 3899 4992
Size: 60 3839 1093
Fragments arranged by size:
3839 1093 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 141 335 1344 2526 2638 4217 4992
Size: 141 194 1009 1182 112 1579 775
Fragments arranged by size:
1579 1182 1009 775 194 141 112
BsrBI CCG'CTC
Cuts at: 0 140 4505 4992
Size: 140 4365 487
Fragments arranged by size:
4365 487 140
BsrFI r'CCGG_y
Cuts at: 0 1305 2267 3644 4992
Size: 1305 962 1377 1348
Fragments arranged by size:
1377 1348 1305 962
BssHII G'CGCG_C
Cuts at: 0 7 4992
Size: 7 4985
BssSI C'ACGA_G
Cuts at: 0 2128 4992
Size: 2128 2864
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 498 4593 4992
Size: 156 342 4095 399
Fragments arranged by size:
4095 399 342 156
BstEII G'GTnAC_C
Cuts at: 0 1212 4992
Size: 1212 3780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 2364 3525 4992
Size: 831 1533 1161 1467
Fragments arranged by size:
1533 1467 1161 831
BstYI r'GATC_y
Cuts at: 0 398 609 1079 4089 4992
Size: 398 211 470 3010 903
Fragments arranged by size:
3010 903 470 398 211
Bsu36I CC'TnA_GG
Cuts at: 0 842 1019 1511 4133 4984 4992
Size: 842 177 492 2622 851 8
Fragments arranged by size:
2622 851 842 492 177 8
BtgI C'CryG_G
Cuts at: 0 81 3132 3849 4992
Size: 81 3051 717 1143
Fragments arranged by size:
3051 1143 717 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1082 4992
Size: 1082 3910
BtsI GCAGTG_nn'
Cuts at: 0 93 265 735 884 1012 1781 1940 2096
Size: 93 172 470 149 128 769 159 156
Cuts at: 2096 2883 3736 4083 4992
Size: 787 853 347 909
Fragments arranged by size:
909 853 787 769 470 347 172 159
156 149 128 93
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1770 1805 2669 2704 2984 3019 4992
Size: 1770 35 864 35 280 35 1973
Fragments arranged by size:
1973 1770 864 280 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 3207 4992
Size: 3207 1785
DrdI GACnn_nn'nnGTC
Cuts at: 0 1640 3895 4992
Size: 1640 2255 1097
Fragments arranged by size:
2255 1640 1097
EaeI y'GGCC_r
Cuts at: 0 755 768 3129 3665 3743 4653 4800 4992
Size: 755 13 2361 536 78 910 147 192
Fragments arranged by size:
2361 910 755 536 192 147 78 13
EarI CTCTTCn'nnn_
Cuts at: 0 608 657 737 1381 2297 3625 4443 4449
Size: 608 49 80 644 916 1328 818 6
Cuts at: 4449 4470 4992
Size: 21 522
Fragments arranged by size:
1328 916 818 644 608 522 80 49
21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4016 4992
Size: 4016 976
EcoICRI GAG'CTC
Cuts at: 0 1366 3139 4992
Size: 1366 1773 1853
Fragments arranged by size:
1853 1773 1366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 693 747 973 1057 1096 1201 1528
Size: 376 317 54 226 84 39 105 327
Cuts at: 1528 2021 2192 2993 3047 3281 3482 3547 3674
Size: 493 171 801 54 234 201 65 127
Cuts at: 3674 3808 3815 4992
Size: 134 7 1177
Fragments arranged by size:
1177 801 493 376 327 317 234 226
201 171 134 127 105 84 65 54
54 39 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 4992
Size: 316 4676
EcoO109I rG'GnC_Cy
Cuts at: 0 51 52 228 559 1657 2467 2939 2952
Size: 51 1 176 331 1098 810 472 13
Cuts at: 2952 2959 3635 3908 4367 4992
Size: 7 676 273 459 625
Fragments arranged by size:
1098 810 676 625 472 459 331 273
176 51 13 7 1
EcoRI G'AATT_C
Cuts at: 0 1599 4992
Size: 1599 3393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3357 3447 4454 4992
Size: 3357 90 1007 538
Fragments arranged by size:
3357 1007 538 90
HaeII r_GCGC'y
Cuts at: 0 115 134 3975 4992
Size: 115 19 3841 1017
Fragments arranged by size:
3841 1017 115 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 1225 1257 2125 2157 3090 3122
Size: 14 32 1179 32 868 32 933 32
Cuts at: 3122 3225 3257 3271 3303 3950 3982 4876 4908
Size: 103 32 14 32 647 32 894 32
Cuts at: 4908 4992
Size: 84
Fragments arranged by size:
1179 933 894 868 647 103 84 32
32 32 32 32 32 32 32 14
14
HincII GTy'rAC
Cuts at: 0 549 1731 1761 1821 1836 1926 2013 2043
Size: 549 1182 30 60 15 90 87 30
Cuts at: 2043 3615 4992
Size: 1572 1377
Fragments arranged by size:
1572 1377 1182 549 90 87 60 30
30 15
HindIII A'AGCT_T
Cuts at: 0 661 4992
Size: 661 4331
KpnI G_GTAC'C
Cuts at: 0 2216 4992
Size: 2216 2776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4325 4919 4992
Size: 4325 594 73
Fragments arranged by size:
4325 594 73
MscI TGG'CCA
Cuts at: 0 757 770 3131 3667 3745 4802 4992
Size: 757 13 2361 536 78 1057 190
Fragments arranged by size:
2361 1057 757 536 190 78 13
MslI CAynn'nnrTG
Cuts at: 0 4727 4992
Size: 4727 265
MspA1I CmG'CkG
Cuts at: 0 83 889 1063 1164 1588 3067 3823 4018
Size: 83 806 174 101 424 1479 756 195
Cuts at: 4018 4197 4406 4992
Size: 179 209 586
Fragments arranged by size:
1479 806 756 586 424 209 195 179
174 101 83
NcoI C'CATG_G
Cuts at: 0 3132 3849 4992
Size: 3132 717 1143
Fragments arranged by size:
3132 1143 717
NsiI A_TGCA'T
Cuts at: 0 830 4039 4992
Size: 830 3209 953
Fragments arranged by size:
3209 953 830
NspI r_CATG'y
Cuts at: 0 2664 2930 2971 3251 4992
Size: 2664 266 41 280 1741
Fragments arranged by size:
2664 1741 280 266 41
PasI CC'CwG_GG
Cuts at: 0 1289 1825 2103 2956 3326 4992
Size: 1289 536 278 853 370 1666
Fragments arranged by size:
1666 1289 853 536 370 278
PciI A'CATG_T
Cuts at: 0 2926 4992
Size: 2926 2066
PflMI CCAn_nnn'nTGG
Cuts at: 0 889 4992
Size: 889 4103
PfoI T'CCnGG_A
Cuts at: 0 812 1107 1127 4992
Size: 812 295 20 3865
Fragments arranged by size:
3865 812 295 20
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 4992
Size: 518 32 4442
Fragments arranged by size:
4442 518 32
PpuMI rG'GwC_Cy
Cuts at: 0 228 2467 3908 4367 4992
Size: 228 2239 1441 459 625
Fragments arranged by size:
2239 1441 625 459 228
PspOMI G'GGCC_C
Cuts at: 0 51 207 559 2938 2952 4011 4992
Size: 51 156 352 2379 14 1059 981
Fragments arranged by size:
2379 1059 981 352 156 51 14
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3441 3473 4992
Size: 3441 32 1519
Fragments arranged by size:
3441 1519 32
PstI C_TGCA'G
Cuts at: 0 257 1788 1935 2423 2649 3828 4228 4992
Size: 257 1531 147 488 226 1179 400 764
Fragments arranged by size:
1531 1179 764 488 400 257 226 147
PvuII CAG'CTG
Cuts at: 0 889 1063 1164 1588 3067 3823 4018 4406
Size: 889 174 101 424 1479 756 195 388
Cuts at: 4406 4992
Size: 586
Fragments arranged by size:
1479 889 756 586 424 388 195 174
101
RsrII CG'GwC_CG
Cuts at: 0 58 4992
Size: 58 4934
SacI G_AGCT'C
Cuts at: 0 1368 3141 4992
Size: 1368 1773 1851
Fragments arranged by size:
1851 1773 1368
SacII CC_GC'GG
Cuts at: 0 84 4992
Size: 84 4908
SalI G'TCGA_C
Cuts at: 0 1729 4992
Size: 1729 3263
SapI GCTCTTCn'nnn_
Cuts at: 0 1381 4443 4992
Size: 1381 3062 549
Fragments arranged by size:
3062 1381 549
SbfI CC_TGCA'GG
Cuts at: 0 2649 4228 4992
Size: 2649 1579 764
Fragments arranged by size:
2649 1579 764
ScaI AGT'ACT
Cuts at: 0 2726 4992
Size: 2726 2266
SexAI A'CCwGG_T
Cuts at: 0 1350 4992
Size: 1350 3642
SfcI C'TryA_G
Cuts at: 0 253 1463 1784 1931 2419 2462 2645 3593
Size: 253 1210 321 147 488 43 183 948
Cuts at: 3593 3824 4224 4407 4992
Size: 231 400 183 585
Fragments arranged by size:
1210 948 585 488 400 321 253 231
183 183 147 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4268 4992
Size: 4268 724
SmaI CCC'GGG
Cuts at: 0 475 1564 4051 4992
Size: 475 1089 2487 941
Fragments arranged by size:
2487 1089 941 475
SmlI C'TyrA_G
Cuts at: 0 976 2443 4242 4992
Size: 976 1467 1799 750
Fragments arranged by size:
1799 1467 976 750
SpeI A'CTAG_T
Cuts at: 0 1147 4992
Size: 1147 3845
SphI G_CATG'C
Cuts at: 0 2664 4992
Size: 2664 2328
StuI AGG'CCT
Cuts at: 0 4138 4992
Size: 4138 854
StyI C'CwwG_G
Cuts at: 0 333 1208 1316 1592 2144 2884 2942 3132
Size: 333 875 108 276 552 740 58 190
Cuts at: 3132 3158 3353 3370 3849 4007 4734 4992
Size: 26 195 17 479 158 727 258
Fragments arranged by size:
875 740 727 552 479 333 276 258
195 190 158 108 58 26 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 953 976 3422 4349 4992
Size: 953 23 2446 927 643
Fragments arranged by size:
2446 953 927 643 23
TatI w'GTAC_w
Cuts at: 0 565 2724 3084 4992
Size: 565 2159 360 1908
Fragments arranged by size:
2159 1908 565 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2108 2140 4062 4094 4155 4187 4992
Size: 2108 32 1922 32 61 32 805
Fragments arranged by size:
2108 1922 805 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 28 4992
Size: 28 4964
XbaI T'CTAG_A
Cuts at: 0 1662 4992
Size: 1662 3330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 115 614 1296 1857 2330 3874 4388 4992
Size: 115 499 682 561 473 1544 514 604
Fragments arranged by size:
1544 682 604 561 514 499 473 115
XmaI C'CCGG_G
Cuts at: 0 473 1562 4049 4992
Size: 473 1089 2487 943
Fragments arranged by size:
2487 1089 943 473
XmnI GAAnn'nnTTC
Cuts at: 0 1520 4992
Size: 1520 3472
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AscI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PsrI PstI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AlfI AloI AseI
AsiSI AvrII BamHI BbeI BmtI BsaHI BsiEI BsiWI
BspHI BsrDI BsrGI BstBI BstZ17I ClaI DraI EagI
EcoRV FseI FspI FspAI HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PmeI PmlI PshAI PsiI PspXI PvuI SanDI SfoI
SgfI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI