Restriction map for
pF1KA0590
(Linear) (Six-base) MAPSORT of: KIAA0590.seq Check: 4042 from: 1 to: 4386
KIAA0590.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1377 2705 4386
Size: 1377 1328 1681
Fragments arranged by size:
1681 1377 1328
AatII G_ACGT'C
Cuts at: 0 3361 4133 4386
Size: 3361 772 253
Fragments arranged by size:
3361 772 253
Acc65I G'GTAC_C
Cuts at: 0 1024 4386
Size: 1024 3362
AclI AA'CG_TT
Cuts at: 0 1478 4386
Size: 1478 2908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1096 2319 3676 4386
Size: 604 492 1223 1357 710
Fragments arranged by size:
1357 1223 710 604 492
AfeI AGC'GCT
Cuts at: 0 2504 4386
Size: 2504 1882
AflIII A'CryG_T
Cuts at: 0 168 545 4229 4386
Size: 168 377 3684 157
Fragments arranged by size:
3684 377 168 157
AhdI GACnn_n'nnGTC
Cuts at: 0 2826 4386
Size: 2826 1560
AleI CACnn'nnGTG
Cuts at: 0 673 1337 4386
Size: 673 664 3049
Fragments arranged by size:
3049 673 664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1345 3609 4386
Size: 1345 2264 777
Fragments arranged by size:
2264 1345 777
AlwNI CAG_nnn'CTG
Cuts at: 0 705 905 2448 2892 3367 3768 4386
Size: 705 200 1543 444 475 401 618
Fragments arranged by size:
1543 705 618 475 444 401 200
ApaI G_GGCC'C
Cuts at: 0 1110 2499 2760 3834 4386
Size: 1110 1389 261 1074 552
Fragments arranged by size:
1389 1110 1074 552 261
ApaLI G'TGCA_C
Cuts at: 0 640 2692 3088 4267 4386
Size: 640 2052 396 1179 119
Fragments arranged by size:
2052 1179 640 396 119
ApoI r'AATT_y
Cuts at: 0 1593 4386
Size: 1593 2793
AvaI C'yCGr_G
Cuts at: 0 1081 1269 2315 2498 2942 3101 3190 4306
Size: 1081 188 1046 183 444 159 89 1116
Cuts at: 4306 4386
Size: 80
Fragments arranged by size:
1116 1081 1046 444 188 183 159 89
80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 155 188 4386
Size: 155 33 4198
Fragments arranged by size:
4198 155 33
BanI G'GyrC_C
Cuts at: 0 68 206 774 1024 2237 2726 2799 4386
Size: 68 138 568 250 1213 489 73 1587
Fragments arranged by size:
1587 1213 568 489 250 138 73 68
BanII G_rGCy'C
Cuts at: 0 337 1110 1130 1973 2499 2760 2840 3187
Size: 337 773 20 843 526 261 80 347
Cuts at: 3187 3834 3964 4249 4386
Size: 647 130 285 137
Fragments arranged by size:
843 773 647 526 347 337 285 261
137 130 80 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1003 2225 2443 4386
Size: 1003 1222 218 1943
Fragments arranged by size:
1943 1222 1003 218
BbvCI CC'TCA_GC
Cuts at: 0 1210 4386
Size: 1210 3176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 684 718 852 886 2668 2702 4386
Size: 684 34 134 34 1782 34 1684
Fragments arranged by size:
1782 1684 684 134 34 34 34
BfrBI ATG'CAT
Cuts at: 0 1467 4386
Size: 1467 2919
BglI GCCn_nnn'nGGC
Cuts at: 0 2494 2545 2554 3667 3913 4386
Size: 2494 51 9 1113 246 473
Fragments arranged by size:
2494 1113 473 246 51 9
BlpI GC'TnA_GC
Cuts at: 0 817 3181 4386
Size: 817 2364 1205
Fragments arranged by size:
2364 1205 817
Bme1580I G_kGCm'C
Cuts at: 0 644 1110 2499 2696 2729 2760 2804 3092
Size: 644 466 1389 197 33 31 44 288
Cuts at: 3092 3108 3834 3870 4271 4386
Size: 16 726 36 401 115
Fragments arranged by size:
1389 726 644 466 401 288 197 115
44 36 33 31 16
BmgBI CAC'GTC
Cuts at: 0 171 2454 4386
Size: 171 2283 1932
Fragments arranged by size:
2283 1932 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 332 4386
Size: 332 4054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 266 1430 2314 2596 2650 2752 2854
Size: 160 106 1164 884 282 54 102 102
Cuts at: 2854 2914 3313 3595 4111 4216 4386
Size: 60 399 282 516 105 170
Fragments arranged by size:
1164 884 516 399 282 282 170 160
106 105 102 102 60 54
Bpu10I CC'TnA_GC
Cuts at: 0 1210 4386
Size: 1210 3176
BsaI GGTCTCn'nnnn_
Cuts at: 0 599 1269 2262 3970 4288 4386
Size: 599 670 993 1708 318 98
Fragments arranged by size:
1708 993 670 599 318 98
BsaAI yAC'GTr
Cuts at: 0 2844 4158 4242 4386
Size: 2844 1314 84 144
Fragments arranged by size:
2844 1314 144 84
BsaHI Gr'CG_yC
Cuts at: 0 421 1450 2005 2321 2582 2915 3358 4130
Size: 421 1029 555 316 261 333 443 772
Cuts at: 4130 4262 4337 4386
Size: 132 75 49
Fragments arranged by size:
1029 772 555 443 421 333 316 261
132 75 49
BsaWI w'CCGG_w
Cuts at: 0 3682 4386
Size: 3682 704
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 324 354 762 792 1526 1556 2221 2251
Size: 324 30 408 30 734 30 665 30
Cuts at: 2251 2938 2968 4386
Size: 687 30 1418
Fragments arranged by size:
1418 734 687 665 408 324 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 935 1531 3085 3238 3517 3528 3568 4213
Size: 935 596 1554 153 279 11 40 645
Cuts at: 4213 4369 4386
Size: 156 17
Fragments arranged by size:
1554 935 645 596 279 156 153 40
17 11
BseYI C'CCAG_C
Cuts at: 0 236 1762 2015 2441 2565 3311 4278 4386
Size: 236 1526 253 426 124 746 967 108
Fragments arranged by size:
1526 967 746 426 253 236 124 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1448 1749 2017 2908 3256 4386
Size: 1448 301 268 891 348 1130
Fragments arranged by size:
1448 1130 891 348 301 268
BsiEI CG_ry'CG
Cuts at: 0 834 2688 4386
Size: 834 1854 1698
Fragments arranged by size:
1854 1698 834
BsiHKAI G_wGCw'C
Cuts at: 0 326 644 1130 1747 2108 2681 2696 2840
Size: 326 318 486 617 361 573 15 144
Cuts at: 2840 3092 3187 3407 4154 4271 4386
Size: 252 95 220 747 117 115
Fragments arranged by size:
747 617 573 486 361 326 318 252
220 144 117 115 95 15
BsmI GAATG_Cn'
Cuts at: 0 3126 4386
Size: 3126 1260
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1441 1861 1996 3349 4386
Size: 1441 420 135 1353 1037
Fragments arranged by size:
1441 1353 1037 420 135
Bsp1286I G_dGCh'C
Cuts at: 0 326 337 644 1110 1130 1747 1973 2108
Size: 326 11 307 466 20 617 226 135
Cuts at: 2108 2499 2681 2696 2729 2760 2804 2840 3092
Size: 391 182 15 33 31 44 36 252
Cuts at: 3092 3108 3187 3407 3834 3870 3964 4154 4249
Size: 16 79 220 427 36 94 190 95
Cuts at: 4249 4271 4386
Size: 22 115
Fragments arranged by size:
617 466 427 391 326 307 252 226
220 190 182 135 115 95 94 79
44 36 36 33 31 22 20 16
15 11
BspEI T'CCGG_A
Cuts at: 0 3682 4386
Size: 3682 704
BspHI T'CATG_A
Cuts at: 0 2121 3164 3794 4386
Size: 2121 1043 630 592
Fragments arranged by size:
2121 1043 630 592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1181 1251 1377 2705 2831 3554 3767 4302
Size: 1181 70 126 1328 126 723 213 535
Cuts at: 4302 4386
Size: 84
Fragments arranged by size:
1328 1181 723 535 213 126 126 84
70
BsrBI CCG'CTC
Cuts at: 0 524 821 1218 4386
Size: 524 297 397 3168
Fragments arranged by size:
3168 524 397 297
BsrDI GCAATG_nn'
Cuts at: 0 1469 2991 4386
Size: 1469 1522 1395
Fragments arranged by size:
1522 1469 1395
BsrFI r'CCGG_y
Cuts at: 0 829 2648 3643 4386
Size: 829 1819 995 743
Fragments arranged by size:
1819 995 829 743
BsrGI T'GTAC_A
Cuts at: 0 767 2594 4386
Size: 767 1827 1792
Fragments arranged by size:
1827 1792 767
BssHII G'CGCG_C
Cuts at: 0 2052 2422 4386
Size: 2052 370 1964
Fragments arranged by size:
2052 1964 370
BssSI C'ACGA_G
Cuts at: 0 436 598 871 4386
Size: 436 162 273 3515
Fragments arranged by size:
3515 436 273 162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 2972 3087 4158 4386
Size: 291 2681 115 1071 228
Fragments arranged by size:
2681 1071 291 228 115
BstBI TT'CG_AA
Cuts at: 0 1512 4386
Size: 1512 2874
BstEII G'GTnAC_C
Cuts at: 0 47 4386
Size: 47 4339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 2565 3271 3291 4347 4386
Size: 675 1890 706 20 1056 39
Fragments arranged by size:
1890 1056 706 675 39 20
Bsu36I CC'TnA_GG
Cuts at: 0 782 3551 4386
Size: 782 2769 835
Fragments arranged by size:
2769 835 782
BtgI C'CryG_G
Cuts at: 0 1199 1298 2490 3524 3909 4256 4386
Size: 1199 99 1192 1034 385 347 130
Fragments arranged by size:
1199 1192 1034 385 347 130 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 831 1698 4386
Size: 831 867 2688
Fragments arranged by size:
2688 867 831
BtsI GCAGTG_nn'
Cuts at: 0 459 1187 2970 4081 4288 4386
Size: 459 728 1783 1111 207 98
Fragments arranged by size:
1783 1111 728 459 207 98
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1135 1170 4386
Size: 1135 35 3216
Fragments arranged by size:
3216 1135 35
DraI TTT'AAA
Cuts at: 0 1629 4386
Size: 1629 2757
DraIII CAC_nnn'GTG
Cuts at: 0 648 4158 4386
Size: 648 3510 228
Fragments arranged by size:
3510 648 228
DrdI GACnn_nn'nnGTC
Cuts at: 0 3250 4386
Size: 3250 1136
EaeI y'GGCC_r
Cuts at: 0 831 878 1016 1202 1250 2456 2546 2555
Size: 831 47 138 186 48 1206 90 9
Cuts at: 2555 3273 3527 3620 3941 4223 4386
Size: 718 254 93 321 282 163
Fragments arranged by size:
1206 831 718 321 282 254 186 163
138 93 90 48 47 9
EagI C'GGCC_G
Cuts at: 0 831 4386
Size: 831 3555
EarI CTCTTCn'nnn_
Cuts at: 0 556 715 1644 2096 4012 4361 4386
Size: 556 159 929 452 1916 349 25
Fragments arranged by size:
1916 929 556 452 349 159 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1702 2530 3118 3526 3792 4306 4386
Size: 1702 828 588 408 266 514 80
Fragments arranged by size:
1702 828 588 514 408 266 80
EcoICRI GAG'CTC
Cuts at: 0 1128 2838 3185 4386
Size: 1128 1710 347 1201
Fragments arranged by size:
1710 1201 1128 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 266 604 1096 1430 2314 2319 2596
Size: 160 106 338 492 334 884 5 277
Cuts at: 2596 2650 2752 2854 2914 3313 3595 3676 4111
Size: 54 102 102 60 399 282 81 435
Cuts at: 4111 4216 4386
Size: 105 170
Fragments arranged by size:
884 492 435 399 338 334 282 277
170 160 106 105 102 102 81 60
54 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3136 3550 3943 3970 4386
Size: 3136 414 393 27 416
Fragments arranged by size:
3136 416 414 393 27
EcoO109I rG'GnC_Cy
Cuts at: 0 492 1107 1433 2495 2661 2757 2820 3288
Size: 492 615 326 1062 166 96 63 468
Cuts at: 3288 3345 3831 4053 4386
Size: 57 486 222 333
Fragments arranged by size:
1062 615 492 486 468 333 326 222
166 96 63 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 883 975 4386
Size: 883 92 3411
Fragments arranged by size:
3411 883 92
FspI TGC'GCA
Cuts at: 0 1321 2701 3002 4386
Size: 1321 1380 301 1384
Fragments arranged by size:
1384 1380 1321 301
HaeII r_GCGC'y
Cuts at: 0 2506 2613 2744 3673 3919 4386
Size: 2506 107 131 929 246 467
Fragments arranged by size:
2506 929 467 246 131 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 579 611 1858 1890 2812 2844 2938 2970
Size: 579 32 1247 32 922 32 94 32
Cuts at: 2970 3343 3375 4386
Size: 373 32 1011
Fragments arranged by size:
1247 1011 922 579 373 94 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1632 4386
Size: 1632 2754
KpnI G_GTAC'C
Cuts at: 0 1028 4386
Size: 1028 3358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 374 2759 2795 3413 4386
Size: 310 64 2385 36 618 973
Fragments arranged by size:
2385 973 618 310 64 36
MscI TGG'CCA
Cuts at: 0 1204 2458 2557 3275 3529 3622 3943 4225
Size: 1204 1254 99 718 254 93 321 282
Cuts at: 4225 4386
Size: 161
Fragments arranged by size:
1254 1204 718 321 282 254 161 99
93
MslI CAynn'nnrTG
Cuts at: 0 319 440 673 1198 1327 1337 2431 2717
Size: 319 121 233 525 129 10 1094 286
Cuts at: 2717 4386
Size: 1669
Fragments arranged by size:
1669 1094 525 319 286 233 129 121
10
MspA1I CmG'CkG
Cuts at: 0 65 517 1021 1739 2445 2492 2565 2592
Size: 65 452 504 718 706 47 73 27
Cuts at: 2592 3383 3471 3993 4386
Size: 791 88 522 393
Fragments arranged by size:
791 718 706 522 504 452 393 88
73 65 47 27
NaeI GCC'GGC
Cuts at: 0 3645 4386
Size: 3645 741
NgoMIV G'CCGG_C
Cuts at: 0 3643 4386
Size: 3643 743
NruI TCG'CGA
Cuts at: 0 3712 4386
Size: 3712 674
NsiI A_TGCA'T
Cuts at: 0 1469 4386
Size: 1469 2917
NspI r_CATG'y
Cuts at: 0 549 1332 2430 2574 3600 4233 4386
Size: 549 783 1098 144 1026 633 153
Fragments arranged by size:
1098 1026 783 633 549 153 144
PasI CC'CwG_GG
Cuts at: 0 1699 4386
Size: 1699 2687
PciI A'CATG_T
Cuts at: 0 545 4229 4386
Size: 545 3684 157
Fragments arranged by size:
3684 545 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 1248 2448 4386
Size: 1248 1200 1938
Fragments arranged by size:
1938 1248 1200
PfoI T'CCnGG_A
Cuts at: 0 1162 2263 4088 4193 4386
Size: 1162 1101 1825 105 193
Fragments arranged by size:
1825 1162 1101 193 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1493 1525 4386
Size: 166 32 1295 32 2861
Fragments arranged by size:
2861 1295 166 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 492 1433 2820 3345 4053 4386
Size: 492 941 1387 525 708 333
Fragments arranged by size:
1387 941 708 525 492 333
PspOMI G'GGCC_C
Cuts at: 0 1106 2495 2756 3830 4386
Size: 1106 1389 261 1074 556
Fragments arranged by size:
1389 1106 1074 556 261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2617 2649 4386
Size: 2617 32 1737
Fragments arranged by size:
2617 1737 32
PstI C_TGCA'G
Cuts at: 0 1678 2752 3470 3764 3998 4386
Size: 1678 1074 718 294 234 388
Fragments arranged by size:
1678 1074 718 388 294 234
PvuI CG_AT'CG
Cuts at: 0 2688 4386
Size: 2688 1698
PvuII CAG'CTG
Cuts at: 0 65 517 2565 2592 3471 3993 4386
Size: 65 452 2048 27 879 522 393
Fragments arranged by size:
2048 879 522 452 393 65 27
SacI G_AGCT'C
Cuts at: 0 1130 2840 3187 4386
Size: 1130 1710 347 1199
Fragments arranged by size:
1710 1199 1130 347
SacII CC_GC'GG
Cuts at: 0 2493 4386
Size: 2493 1893
SapI GCTCTTCn'nnn_
Cuts at: 0 556 2096 4386
Size: 556 1540 2290
Fragments arranged by size:
2290 1540 556
SexAI A'CCwGG_T
Cuts at: 0 494 4386
Size: 494 3892
SfcI C'TryA_G
Cuts at: 0 1668 1674 2748 3328 3466 3760 3994 4185
Size: 1668 6 1074 580 138 294 234 191
Cuts at: 4185 4386
Size: 201
Fragments arranged by size:
1668 1074 580 294 234 201 191 138
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2545 2554 4386
Size: 2545 9 1832
Fragments arranged by size:
2545 1832 9
SmaI CCC'GGG
Cuts at: 0 1083 2317 2944 3103 4386
Size: 1083 1234 627 159 1283
Fragments arranged by size:
1283 1234 1083 627 159
SphI G_CATG'C
Cuts at: 0 2574 3600 4386
Size: 2574 1026 786
Fragments arranged by size:
2574 1026 786
StuI AGG'CCT
Cuts at: 0 3112 3928 4386
Size: 3112 816 458
Fragments arranged by size:
3112 816 458
StyI C'CwwG_G
Cuts at: 0 1131 1355 2459 3284 3530 3821 3944 3950
Size: 1131 224 1104 825 246 291 123 6
Cuts at: 3950 4058 4220 4386
Size: 108 162 166
Fragments arranged by size:
1131 1104 825 291 246 224 166 162
123 108 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2225 3325 3937 4386
Size: 2225 1100 612 449
Fragments arranged by size:
2225 1100 612 449
TatI w'GTAC_w
Cuts at: 0 767 2594 3632 4386
Size: 767 1827 1038 754
Fragments arranged by size:
1827 1038 767 754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 324 356 762 794 1373 1405 2221 2253
Size: 324 32 406 32 579 32 816 32
Cuts at: 2253 4286 4318 4386
Size: 2033 32 68
Fragments arranged by size:
2033 816 579 406 324 68 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1872 4386
Size: 1872 2514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2358 2466 3963 4386
Size: 2358 108 1497 423
Fragments arranged by size:
2358 1497 423 108
XmaI C'CCGG_G
Cuts at: 0 1081 2315 2942 3101 4386
Size: 1081 1234 627 159 1285
Fragments arranged by size:
1285 1234 1081 627 159
XmnI GAAnn'nnTTC
Cuts at: 0 1951 2629 4021 4386
Size: 1951 678 1392 365
Fragments arranged by size:
1951 1392 678 365
ZraI GAC'GTC
Cuts at: 0 3359 4131 4386
Size: 3359 772 255
Fragments arranged by size:
3359 772 255
Enzymes that do cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI
BanI BanII BbsI BbvCI BcgI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NaeI NgoMIV NruI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII SacI SacII SapI SexAI SfcI SfiI SmaI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI AflII AgeI AloI AscI AseI AsiSI AvrII
BamHI BbeI BciVI BclI BglII BmtI BplI BpuEI
BsaBI BsiWI BstYI BstZ17I ClaI EcoRI EcoRV FseI
FspAI HincII HpaI KasI MfeI MluI NarI NcoI
NdeI NheI NotI PacI PmeI PmlI PshAI PsiI
PspXI RsrII SalI SanDI SbfI ScaI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI