Restriction map for
pF1KSDA0576
(Linear) (Six-base) MAPSORT of: KIAA0576.seq Check: 4209 from: 1 to: 3183
KIAA0576.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2386 3183
Size: 2386 797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 3183
Size: 609 2574
AflIII A'CryG_T
Cuts at: 0 482 1644 3183
Size: 482 1162 1539
Fragments arranged by size:
1539 1162 482
AhdI GACnn_n'nnGTC
Cuts at: 0 12 1159 3183
Size: 12 1147 2024
Fragments arranged by size:
2024 1147 12
AleI CACnn'nnGTG
Cuts at: 0 487 1317 3183
Size: 487 830 1866
Fragments arranged by size:
1866 830 487
ApaLI G'TGCA_C
Cuts at: 0 2323 2408 3183
Size: 2323 85 775
Fragments arranged by size:
2323 775 85
ApoI r'AATT_y
Cuts at: 0 355 658 1069 1146 1267 1347 1654 2289
Size: 355 303 411 77 121 80 307 635
Cuts at: 2289 2915 3121 3183
Size: 626 206 62
Fragments arranged by size:
635 626 411 355 307 303 206 121
80 77 62
AseI AT'TA_AT
Cuts at: 0 443 3183
Size: 443 2740
AvaI C'yCGr_G
Cuts at: 0 9 3183
Size: 9 3174
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1896 1929 3183
Size: 1896 33 1254
Fragments arranged by size:
1896 1254 33
BamHI G'GATC_C
Cuts at: 0 1227 3183
Size: 1227 1956
BanI G'GyrC_C
Cuts at: 0 548 2374 3094 3183
Size: 548 1826 720 89
Fragments arranged by size:
1826 720 548 89
BanII G_rGCy'C
Cuts at: 0 989 3183
Size: 989 2194
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 2983 3183
Size: 89 2894 200
Fragments arranged by size:
2894 200 89
BclI T'GATC_A
Cuts at: 0 1779 3183
Size: 1779 1404
BfrBI ATG'CAT
Cuts at: 0 2880 3183
Size: 2880 303
Bme1580I G_kGCm'C
Cuts at: 0 551 1572 2327 2412 3183
Size: 551 1021 755 85 771
Fragments arranged by size:
1021 771 755 551 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 859 2447 3183
Size: 859 1588 736
Fragments arranged by size:
1588 859 736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3008 3183
Size: 3008 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 1336 2974 3183
Size: 23 1313 1638 209
Fragments arranged by size:
1638 1313 209 23
Bpu10I CC'TnA_GC
Cuts at: 0 2590 3183
Size: 2590 593
BsaI GGTCTCn'nnnn_
Cuts at: 0 2137 3183
Size: 2137 1046
BsaAI yAC'GTr
Cuts at: 0 485 3183
Size: 485 2698
BsaBI GATnn'nnATC
Cuts at: 0 1100 3183
Size: 1100 2083
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2341 2371 3183
Size: 2341 30 812
Fragments arranged by size:
2341 812 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 488 811 2074 2359 2362 3183
Size: 488 323 1263 285 3 821
Fragments arranged by size:
1263 821 488 323 285 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2364 3183
Size: 2364 819
BsiHKAI G_wGCw'C
Cuts at: 0 989 1917 2066 2327 2412 3183
Size: 989 928 149 261 85 771
Fragments arranged by size:
989 928 771 261 149 85
BsmI GAATG_Cn'
Cuts at: 0 304 1429 3183
Size: 304 1125 1754
Fragments arranged by size:
1754 1125 304
Bsp1286I G_dGCh'C
Cuts at: 0 551 989 1572 1917 2066 2327 2412 3183
Size: 551 438 583 345 149 261 85 771
Fragments arranged by size:
771 583 551 438 345 261 149 85
BspHI T'CATG_A
Cuts at: 0 2394 3183
Size: 2394 789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2386 2651 3183
Size: 2386 265 532
Fragments arranged by size:
2386 532 265
BsrBI CCG'CTC
Cuts at: 0 2778 3183
Size: 2778 405
BsrDI GCAATG_nn'
Cuts at: 0 1322 1825 1959 2433 3183
Size: 1322 503 134 474 750
Fragments arranged by size:
1322 750 503 474 134
BsrGI T'GTAC_A
Cuts at: 0 2489 3183
Size: 2489 694
BstYI r'GATC_y
Cuts at: 0 1227 2470 3183
Size: 1227 1243 713
Fragments arranged by size:
1243 1227 713
Bsu36I CC'TnA_GG
Cuts at: 0 50 3183
Size: 50 3133
BtsI GCAGTG_nn'
Cuts at: 0 156 967 989 1869 1917 3183
Size: 156 811 22 880 48 1266
Fragments arranged by size:
1266 880 811 156 48 22
DraI TTT'AAA
Cuts at: 0 1352 1577 3183
Size: 1352 225 1606
Fragments arranged by size:
1606 1352 225
EaeI y'GGCC_r
Cuts at: 0 2886 3183
Size: 2886 297
EarI CTCTTCn'nnn_
Cuts at: 0 155 284 465 1039 3183
Size: 155 129 181 574 2144
Fragments arranged by size:
2144 574 181 155 129
EcoICRI GAG'CTC
Cuts at: 0 987 3183
Size: 987 2196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 609 1336 2974 3183
Size: 23 586 727 1638 209
Fragments arranged by size:
1638 727 586 209 23
EcoRI G'AATT_C
Cuts at: 0 1146 3183
Size: 1146 2037
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1145 1177 2671 2703 3183
Size: 30 1115 32 1494 32 480
Fragments arranged by size:
1494 1115 480 32 32 30
HincII GTy'rAC
Cuts at: 0 65 1619 2159 2196 3183
Size: 65 1554 540 37 987
Fragments arranged by size:
1554 987 540 65 37
HpaI GTT'AAC
Cuts at: 0 1619 2159 3183
Size: 1619 540 1024
Fragments arranged by size:
1619 1024 540
MfeI C'AATT_G
Cuts at: 0 2763 3183
Size: 2763 420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1235 1579 3183
Size: 622 613 344 1604
Fragments arranged by size:
1604 622 613 344
MslI CAynn'nnrTG
Cuts at: 0 335 487 1317 1370 1979 2875 3183
Size: 335 152 830 53 609 896 308
Fragments arranged by size:
896 830 609 335 308 152 53
MspA1I CmG'CkG
Cuts at: 0 43 1910 3183
Size: 43 1867 1273
Fragments arranged by size:
1867 1273 43
NdeI CA'TA_TG
Cuts at: 0 812 2872 3183
Size: 812 2060 311
Fragments arranged by size:
2060 812 311
NsiI A_TGCA'T
Cuts at: 0 2882 3183
Size: 2882 301
NspI r_CATG'y
Cuts at: 0 334 764 1468 1607 1648 1878 2884 3183
Size: 334 430 704 139 41 230 1006 299
Fragments arranged by size:
1006 704 430 334 299 230 139 41
PciI A'CATG_T
Cuts at: 0 1644 3183
Size: 1644 1539
PmlI CAC'GTG
Cuts at: 0 485 3183
Size: 485 2698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2640 2672 3183
Size: 2640 32 511
Fragments arranged by size:
2640 511 32
PsiI TTA'TAA
Cuts at: 0 653 1493 2795 3183
Size: 653 840 1302 388
Fragments arranged by size:
1302 840 653 388
PstI C_TGCA'G
Cuts at: 0 1924 2266 3183
Size: 1924 342 917
Fragments arranged by size:
1924 917 342
PvuII CAG'CTG
Cuts at: 0 43 1910 3183
Size: 43 1867 1273
Fragments arranged by size:
1867 1273 43
SacI G_AGCT'C
Cuts at: 0 989 3183
Size: 989 2194
SapI GCTCTTCn'nnn_
Cuts at: 0 155 1039 3183
Size: 155 884 2144
Fragments arranged by size:
2144 884 155
SexAI A'CCwGG_T
Cuts at: 0 1411 3183
Size: 1411 1772
SfcI C'TryA_G
Cuts at: 0 1028 1034 1920 2262 2838 3183
Size: 1028 6 886 342 576 345
Fragments arranged by size:
1028 886 576 345 342 6
SmaI CCC'GGG
Cuts at: 0 11 3183
Size: 11 3172
SmlI C'TyrA_G
Cuts at: 0 3172 3183
Size: 3172 11
SphI G_CATG'C
Cuts at: 0 334 2884 3183
Size: 334 2550 299
Fragments arranged by size:
2550 334 299
SspI AAT'ATT
Cuts at: 0 192 3183
Size: 192 2991
StyI C'CwwG_G
Cuts at: 0 2724 3183
Size: 2724 459
SwaI ATTT'AAAT
Cuts at: 0 1577 3183
Size: 1577 1606
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 841 1144 3082 3183
Size: 477 364 303 1938 101
Fragments arranged by size:
1938 477 364 303 101
TatI w'GTAC_w
Cuts at: 0 2489 3183
Size: 2489 694
XbaI T'CTAG_A
Cuts at: 0 2892 3183
Size: 2892 291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2161 3138 3183
Size: 2161 977 45
Fragments arranged by size:
2161 977 45
XmaI C'CCGG_G
Cuts at: 0 9 3183
Size: 9 3174
XmnI GAAnn'nnTTC
Cuts at: 0 359 574 829 2042 3183
Size: 359 215 255 1213 1141
Fragments arranged by size:
1213 1141 359 255 215
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI ApaLI ApoI AseI
AvaI BaeI BamHI BanI BanII BbsI BclI BfrBI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BstYI Bsu36I BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoRI Hin4I HincII HpaI MfeI
MmeI MslI MspA1I NdeI NsiI NspI PciI PmlI
PpiI PsiI PstI PvuII SacI SapI SexAI SfcI
SmaI SmlI SphI SspI StyI SwaI TaqII TatI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AlwNI ApaI AscI AsiSI AvrII BbeI BbvCI
BcgI BciVI BglI BglII BlpI BmgBI BmtI BpuEI
BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI StuI TstI Tth111I XhoI ZraI