Restriction map for
pF1KA0575
(Linear) (Six-base) MAPSORT of: KIAA0575.seq Check: 3157 from: 1 to: 2841
KIAA0575.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 2222 2513 2841
Size: 2222 291 328
Fragments arranged by size:
2222 328 291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1533 2262 2503 2841
Size: 63 1470 729 241 338
Fragments arranged by size:
1470 729 338 241 63
AfeI AGC'GCT
Cuts at: 0 413 2841
Size: 413 2428
AflIII A'CryG_T
Cuts at: 0 2009 2402 2841
Size: 2009 393 439
Fragments arranged by size:
2009 439 393
AgeI A'CCGG_T
Cuts at: 0 2201 2841
Size: 2201 640
AleI CACnn'nnGTG
Cuts at: 0 1105 1397 2841
Size: 1105 292 1444
Fragments arranged by size:
1444 1105 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 838 1033 1484 2841
Size: 838 195 451 1357
Fragments arranged by size:
1357 838 451 195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2587 2619 2841
Size: 2587 32 222
Fragments arranged by size:
2587 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 186 2430 2841
Size: 186 2244 411
Fragments arranged by size:
2244 411 186
ApaLI G'TGCA_C
Cuts at: 0 2143 2239 2841
Size: 2143 96 602
Fragments arranged by size:
2143 602 96
AvaI C'yCGr_G
Cuts at: 0 530 574 669 1858 2102 2746 2841
Size: 530 44 95 1189 244 644 95
Fragments arranged by size:
1189 644 530 244 95 95 44
BanI G'GyrC_C
Cuts at: 0 693 942 1051 1693 2841
Size: 693 249 109 642 1148
Fragments arranged by size:
1148 693 642 249 109
BanII G_rGCy'C
Cuts at: 0 510 676 1667 1859 2126 2563 2716 2747
Size: 510 166 991 192 267 437 153 31
Cuts at: 2747 2841
Size: 94
Fragments arranged by size:
991 510 437 267 192 166 153 94
31
BbeI G_GCGC'C
Cuts at: 0 697 2841
Size: 697 2144
BbsI GAAGACnn'nnnn_
Cuts at: 0 762 1051 1304 1539 2810 2841
Size: 762 289 253 235 1271 31
Fragments arranged by size:
1271 762 289 253 235 31
BlpI GC'TnA_GC
Cuts at: 0 274 434 889 1021 1177 2841
Size: 274 160 455 132 156 1664
Fragments arranged by size:
1664 455 274 160 156 132
Bme1580I G_kGCm'C
Cuts at: 0 947 2147 2243 2388 2841
Size: 947 1200 96 145 453
Fragments arranged by size:
1200 947 453 145 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 723 1778 2367 2494 2841
Size: 723 1055 589 127 347
Fragments arranged by size:
1055 723 589 347 127
BmtI G_CTAG'C
Cuts at: 0 2651 2841
Size: 2651 190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2145 2841
Size: 2145 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1156 1219 1580 1591 1823 1873 2455
Size: 73 1083 63 361 11 232 50 582
Cuts at: 2455 2547 2761 2841
Size: 92 214 80
Fragments arranged by size:
1083 582 361 232 214 92 80 73
63 50 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 24 2841
Size: 24 2817
BsaAI yAC'GTr
Cuts at: 0 710 1710 2142 2841
Size: 710 1000 432 699
Fragments arranged by size:
1000 710 699 432
BsaBI GATnn'nnATC
Cuts at: 0 1135 2841
Size: 1135 1706
BsaHI Gr'CG_yC
Cuts at: 0 583 694 1341 2841
Size: 583 111 647 1500
Fragments arranged by size:
1500 647 583 111
BsaWI w'CCGG_w
Cuts at: 0 403 2201 2708 2841
Size: 403 1798 507 133
Fragments arranged by size:
1798 507 403 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1620 1650 2841
Size: 1620 30 1191
Fragments arranged by size:
1620 1191 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 313 381 511 633 654 657 672
Size: 141 172 68 130 122 21 3 15
Cuts at: 672 1138 1141 1510 1891 2841
Size: 466 3 369 381 950
Fragments arranged by size:
950 466 381 369 172 141 130 122
68 21 15 3 3
BseYI C'CCAG_C
Cuts at: 0 186 303 476 587 653 952 1881 1969
Size: 186 117 173 111 66 299 929 88
Cuts at: 1969 2073 2423 2841
Size: 104 350 418
Fragments arranged by size:
929 418 350 299 186 173 117 111
104 88 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 1492 2206 2841
Size: 1444 48 714 635
Fragments arranged by size:
1444 714 635 48
BsiEI CG_ry'CG
Cuts at: 0 1650 2363 2732 2812 2841
Size: 1650 713 369 80 29
Fragments arranged by size:
1650 713 369 80 29
BsiHKAI G_wGCw'C
Cuts at: 0 652 736 1538 1781 1859 2147 2243 2563
Size: 652 84 802 243 78 288 96 320
Cuts at: 2563 2716 2747 2841
Size: 153 31 94
Fragments arranged by size:
802 652 320 288 243 153 96 94
84 78 31
Bsp1286I G_dGCh'C
Cuts at: 0 510 652 676 736 947 1538 1667 1781
Size: 510 142 24 60 211 591 129 114
Cuts at: 1781 1859 2126 2147 2243 2388 2563 2716 2747
Size: 78 267 21 96 145 175 153 31
Cuts at: 2747 2841
Size: 94
Fragments arranged by size:
591 510 267 211 175 153 145 142
129 114 96 94 78 60 31 24
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 1067 2240 2841
Size: 143 924 1173 601
Fragments arranged by size:
1173 924 601 143
BsrBI CCG'CTC
Cuts at: 0 362 1863 2814 2841
Size: 362 1501 951 27
Fragments arranged by size:
1501 951 362 27
BsrDI GCAATG_nn'
Cuts at: 0 1691 2841
Size: 1691 1150
BsrFI r'CCGG_y
Cuts at: 0 610 703 929 1645 1868 2201 2248 2264
Size: 610 93 226 716 223 333 47 16
Cuts at: 2264 2367 2807 2841
Size: 103 440 34
Fragments arranged by size:
716 610 440 333 226 223 103 93
47 34 16
BssSI C'ACGA_G
Cuts at: 0 784 1591 2841
Size: 784 807 1250
Fragments arranged by size:
1250 807 784
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2028 2841
Size: 2028 813
BstEII G'GTnAC_C
Cuts at: 0 636 2841
Size: 636 2205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 484 960 2841
Size: 484 476 1881
Fragments arranged by size:
1881 484 476
BstYI r'GATC_y
Cuts at: 0 1499 2533 2841
Size: 1499 1034 308
Fragments arranged by size:
1499 1034 308
Bsu36I CC'TnA_GG
Cuts at: 0 604 2841
Size: 604 2237
BtgI C'CryG_G
Cuts at: 0 483 689 2357 2841
Size: 483 206 1668 484
Fragments arranged by size:
1668 484 483 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2789 2841
Size: 2789 52
BtsI GCAGTG_nn'
Cuts at: 0 822 940 1411 2716 2841
Size: 822 118 471 1305 125
Fragments arranged by size:
1305 822 471 125 118
DraI TTT'AAA
Cuts at: 0 2579 2841
Size: 2579 262
DraIII CAC_nnn'GTG
Cuts at: 0 2502 2841
Size: 2502 339
EaeI y'GGCC_r
Cuts at: 0 554 612 1189 1647 2310 2809 2841
Size: 554 58 577 458 663 499 32
Fragments arranged by size:
663 577 554 499 458 58 32
EagI C'GGCC_G
Cuts at: 0 1647 2809 2841
Size: 1647 1162 32
Fragments arranged by size:
1647 1162 32
EarI CTCTTCn'nnn_
Cuts at: 0 325 2470 2841
Size: 325 2145 371
Fragments arranged by size:
2145 371 325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 868 2725 2841
Size: 868 1857 116
Fragments arranged by size:
1857 868 116
EcoICRI GAG'CTC
Cuts at: 0 1857 2561 2714 2745 2841
Size: 1857 704 153 31 96
Fragments arranged by size:
1857 704 153 96 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 73 1156 1219 1533 1580 1591 1823
Size: 63 10 1083 63 314 47 11 232
Cuts at: 1823 1873 2262 2455 2503 2547 2761 2841
Size: 50 389 193 48 44 214 80
Fragments arranged by size:
1083 389 314 232 214 193 80 63
63 50 48 47 44 11 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 111 826 1828 2841
Size: 111 715 1002 1013
Fragments arranged by size:
1013 1002 715 111
EcoO109I rG'GnC_Cy
Cuts at: 0 356 527 714 1326 2318 2567 2841
Size: 356 171 187 612 992 249 274
Fragments arranged by size:
992 612 356 274 249 187 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1236 2841
Size: 1236 1605
HaeII r_GCGC'y
Cuts at: 0 415 697 2249 2419 2841
Size: 415 282 1552 170 422
Fragments arranged by size:
1552 422 415 282 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 1484 1516 1574 1606 1620 1652
Size: 361 32 1091 32 58 32 14 32
Cuts at: 1652 1948 1980 2698 2730 2822 2841
Size: 296 32 718 32 92 19
Fragments arranged by size:
1091 718 361 296 92 58 32 32
32 32 32 32 19 14
HincII GTy'rAC
Cuts at: 0 1962 2514 2841
Size: 1962 552 327
Fragments arranged by size:
1962 552 327
KasI G'GCGC_C
Cuts at: 0 693 2841
Size: 693 2148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1651 1663 1979 2841
Size: 1651 12 316 862
Fragments arranged by size:
1651 862 316 12
MscI TGG'CCA
Cuts at: 0 1191 2841
Size: 1191 1650
MslI CAynn'nnrTG
Cuts at: 0 799 1105 1397 1658 1774 2841
Size: 799 306 292 261 116 1067
Fragments arranged by size:
1067 799 306 292 261 116
MspA1I CmG'CkG
Cuts at: 0 657 700 1025 1443 1506 1569 2135 2359
Size: 657 43 325 418 63 63 566 224
Cuts at: 2359 2475 2588 2841
Size: 116 113 253
Fragments arranged by size:
657 566 418 325 253 224 116 113
63 63 43
NaeI GCC'GGC
Cuts at: 0 705 2250 2369 2841
Size: 705 1545 119 472
Fragments arranged by size:
1545 705 472 119
NarI GG'CG_CC
Cuts at: 0 694 2841
Size: 694 2147
NgoMIV G'CCGG_C
Cuts at: 0 703 2248 2367 2841
Size: 703 1545 119 474
Fragments arranged by size:
1545 703 474 119
NheI G'CTAG_C
Cuts at: 0 2647 2841
Size: 2647 194
NspI r_CATG'y
Cuts at: 0 466 1581 2841
Size: 466 1115 1260
Fragments arranged by size:
1260 1115 466
PasI CC'CwG_GG
Cuts at: 0 453 2841
Size: 453 2388
PflMI CCAn_nnn'nTGG
Cuts at: 0 1419 1686 1851 2841
Size: 1419 267 165 990
Fragments arranged by size:
1419 990 267 165
PfoI T'CCnGG_A
Cuts at: 0 1081 1312 2841
Size: 1081 231 1529
Fragments arranged by size:
1529 1081 231
PmlI CAC'GTG
Cuts at: 0 710 1710 2142 2841
Size: 710 1000 432 699
Fragments arranged by size:
1000 710 699 432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1649 1681 2746 2778 2841
Size: 1649 32 1065 32 63
Fragments arranged by size:
1649 1065 63 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 356 714 1326 2318 2841
Size: 356 358 612 992 523
Fragments arranged by size:
992 612 523 358 356
PshAI GACnn'nnGTC
Cuts at: 0 2731 2841
Size: 2731 110
PsiI TTA'TAA
Cuts at: 0 1745 2841
Size: 1745 1096
PspXI vC'TCGA_Gb
Cuts at: 0 1858 2746 2841
Size: 1858 888 95
Fragments arranged by size:
1858 888 95
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2173 2205 2841
Size: 2173 32 636
Fragments arranged by size:
2173 636 32
PstI C_TGCA'G
Cuts at: 0 722 1064 2450 2841
Size: 722 342 1386 391
Fragments arranged by size:
1386 722 391 342
PvuII CAG'CTG
Cuts at: 0 657 1443 1506 1569 2588 2841
Size: 657 786 63 63 1019 253
Fragments arranged by size:
1019 786 657 253 63 63
SacI G_AGCT'C
Cuts at: 0 1859 2563 2716 2747 2841
Size: 1859 704 153 31 94
Fragments arranged by size:
1859 704 153 94 31
SacII CC_GC'GG
Cuts at: 0 2360 2841
Size: 2360 481
SalI G'TCGA_C
Cuts at: 0 2512 2841
Size: 2512 329
SbfI CC_TGCA'GG
Cuts at: 0 722 2841
Size: 722 2119
SfcI C'TryA_G
Cuts at: 0 597 718 1060 2446 2841
Size: 597 121 342 1386 395
Fragments arranged by size:
1386 597 395 342 121
SfoI GGC'GCC
Cuts at: 0 695 2841
Size: 695 2146
SmaI CCC'GGG
Cuts at: 0 532 576 2104 2841
Size: 532 44 1528 737
Fragments arranged by size:
1528 737 532 44
SmlI C'TyrA_G
Cuts at: 0 1858 2746 2841
Size: 1858 888 95
Fragments arranged by size:
1858 888 95
SphI G_CATG'C
Cuts at: 0 466 1581 2841
Size: 466 1115 1260
Fragments arranged by size:
1260 1115 466
SspI AAT'ATT
Cuts at: 0 1753 2841
Size: 1753 1088
StuI AGG'CCT
Cuts at: 0 250 724 748 2841
Size: 250 474 24 2093
Fragments arranged by size:
2093 474 250 24
StyI C'CwwG_G
Cuts at: 0 173 224 251 395 743 827 1529 2336
Size: 173 51 27 144 348 84 702 807
Cuts at: 2336 2841
Size: 505
Fragments arranged by size:
807 702 505 348 173 144 84 51
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 624 777 846 2513 2841
Size: 624 153 69 1667 328
Fragments arranged by size:
1667 624 328 153 69
TatI w'GTAC_w
Cuts at: 0 1415 2189 2375 2841
Size: 1415 774 186 466
Fragments arranged by size:
1415 774 466 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 10 42 1035 1067 2317 2349 2841
Size: 10 32 993 32 1250 32 492
Fragments arranged by size:
1250 993 492 32 32 32 10
XhoI C'TCGA_G
Cuts at: 0 1858 2746 2841
Size: 1858 888 95
Fragments arranged by size:
1858 888 95
XmaI C'CCGG_G
Cuts at: 0 530 574 2102 2841
Size: 530 44 1528 739
Fragments arranged by size:
1528 739 530 44
XmnI GAAnn'nnTTC
Cuts at: 0 2203 2841
Size: 2203 638
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AleI AlfI AloI
AlwNI ApaLI AvaI BanI BanII BbeI BbsI BlpI
Bme1580I BmrI BmtI BplI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuII SacI SacII SalI SbfI SfcI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI Bpu10I BpuEI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI
NotI NruI NsiI PacI PciI PmeI PspOMI PvuI
RsrII SanDI SapI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SwaI Tth111I XbaI XcmI ZraI