Restriction map for
pF1KA0569
(Linear) (Six-base) MAPSORT of: KIAA0569.seq Check: 3889 from: 1 to: 3642
KIAA0569.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 908 3642
Size: 908 2734
AccI GT'mk_AC
Cuts at: 0 2090 3642
Size: 2090 1552
AclI AA'CG_TT
Cuts at: 0 951 1800 3642
Size: 951 849 1842
Fragments arranged by size:
1842 951 849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 697 1460 3642
Size: 484 213 763 2182
Fragments arranged by size:
2182 763 484 213
AfeI AGC'GCT
Cuts at: 0 664 3187 3642
Size: 664 2523 455
Fragments arranged by size:
2523 664 455
AflIII A'CryG_T
Cuts at: 0 216 743 1964 2915 3642
Size: 216 527 1221 951 727
Fragments arranged by size:
1221 951 727 527 216
AhdI GACnn_n'nnGTC
Cuts at: 0 1965 2916 3642
Size: 1965 951 726
Fragments arranged by size:
1965 951 726
AleI CACnn'nnGTG
Cuts at: 0 914 1316 2246 3081 3642
Size: 914 402 930 835 561
Fragments arranged by size:
930 914 835 561 402
AlwNI CAG_nnn'CTG
Cuts at: 0 106 3642
Size: 106 3536
ApoI r'AATT_y
Cuts at: 0 905 1445 2027 2056 2103 2602 2610 3642
Size: 905 540 582 29 47 499 8 1032
Fragments arranged by size:
1032 905 582 540 499 47 29 8
AvaI C'yCGr_G
Cuts at: 0 766 3147 3198 3642
Size: 766 2381 51 444
Fragments arranged by size:
2381 766 444 51
BamHI G'GATC_C
Cuts at: 0 1476 3522 3642
Size: 1476 2046 120
Fragments arranged by size:
2046 1476 120
BanI G'GyrC_C
Cuts at: 0 1558 3642
Size: 1558 2084
BanII G_rGCy'C
Cuts at: 0 2006 2432 3205 3642
Size: 2006 426 773 437
Fragments arranged by size:
2006 773 437 426
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 350 1528 2983 3626 3642
Size: 128 222 1178 1455 643 16
Fragments arranged by size:
1455 1178 643 222 128 16
BbvCI CC'TCA_GC
Cuts at: 0 140 3642
Size: 140 3502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 491 747 781 3642
Size: 457 34 256 34 2861
Fragments arranged by size:
2861 457 256 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3510 3642
Size: 3510 132
BfrBI ATG'CAT
Cuts at: 0 2998 3642
Size: 2998 644
BlpI GC'TnA_GC
Cuts at: 0 2438 3642
Size: 2438 1204
Bme1580I G_kGCm'C
Cuts at: 0 197 937 3299 3642
Size: 197 740 2362 343
Fragments arranged by size:
2362 740 343 197
BmrI ACTGGGnnnn_n'
Cuts at: 0 1095 2638 3642
Size: 1095 1543 1004
Fragments arranged by size:
1543 1095 1004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 245 2025 2360 3642
Size: 245 1780 335 1282
Fragments arranged by size:
1780 1282 335 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 593 1603 2316 2416 2843 3575 3642
Size: 478 115 1010 713 100 427 732 67
Fragments arranged by size:
1010 732 713 478 427 115 100 67
Bpu10I CC'TnA_GC
Cuts at: 0 140 3642
Size: 140 3502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1623 3120 3642
Size: 1623 1497 522
Fragments arranged by size:
1623 1497 522
BsaI GGTCTCn'nnnn_
Cuts at: 0 2195 3642
Size: 2195 1447
BsaAI yAC'GTr
Cuts at: 0 219 1678 3642
Size: 219 1459 1964
Fragments arranged by size:
1964 1459 219
BsaBI GATnn'nnATC
Cuts at: 0 3521 3642
Size: 3521 121
BsaWI w'CCGG_w
Cuts at: 0 1539 3642
Size: 1539 2103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1868 1898 2334 2364 2932 2962 3642
Size: 1868 30 436 30 568 30 680
Fragments arranged by size:
1868 680 568 436 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 556 1891 2258 2440 3271 3388 3487
Size: 505 51 1335 367 182 831 117 99
Cuts at: 3487 3496 3642
Size: 9 146
Fragments arranged by size:
1335 831 505 367 182 146 117 99
51 9
BseYI C'CCAG_C
Cuts at: 0 761 3450 3642
Size: 761 2689 192
Fragments arranged by size:
2689 761 192
BsiEI CG_ry'CG
Cuts at: 0 651 3642
Size: 651 2991
BsiHKAI G_wGCw'C
Cuts at: 0 686 2432 2907 3134 3419 3642
Size: 686 1746 475 227 285 223
Fragments arranged by size:
1746 686 475 285 227 223
BsmI GAATG_Cn'
Cuts at: 0 414 3642
Size: 414 3228
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 3642
Size: 169 3473
Bsp1286I G_dGCh'C
Cuts at: 0 197 686 937 2006 2432 2907 3134 3205
Size: 197 489 251 1069 426 475 227 71
Cuts at: 3205 3299 3419 3642
Size: 94 120 223
Fragments arranged by size:
1069 489 475 426 251 227 223 197
120 94 71
BspHI T'CATG_A
Cuts at: 0 1848 3642
Size: 1848 1794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 643 820 908 3642
Size: 602 41 177 88 2734
Fragments arranged by size:
2734 602 177 88 41
BsrBI CCG'CTC
Cuts at: 0 771 3261 3642
Size: 771 2490 381
Fragments arranged by size:
2490 771 381
BsrDI GCAATG_nn'
Cuts at: 0 2033 3642
Size: 2033 1609
BssHII G'CGCG_C
Cuts at: 0 3283 3642
Size: 3283 359
BssSI C'ACGA_G
Cuts at: 0 700 3418 3613 3642
Size: 700 2718 195 29
Fragments arranged by size:
2718 700 195 29
BstAPI GCAn_nnn'nTGC
Cuts at: 0 813 1987 3642
Size: 813 1174 1655
Fragments arranged by size:
1655 1174 813
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 2040 3642
Size: 165 1875 1602
Fragments arranged by size:
1875 1602 165
BstYI r'GATC_y
Cuts at: 0 1476 3522 3642
Size: 1476 2046 120
Fragments arranged by size:
2046 1476 120
Bsu36I CC'TnA_GG
Cuts at: 0 539 1493 2051 3353 3642
Size: 539 954 558 1302 289
Fragments arranged by size:
1302 954 558 539 289
BtgI C'CryG_G
Cuts at: 0 3566 3642
Size: 3566 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 482 3642
Size: 482 3160
BtsI GCAGTG_nn'
Cuts at: 0 513 3642
Size: 513 3129
DraIII CAC_nnn'GTG
Cuts at: 0 857 3642
Size: 857 2785
EaeI y'GGCC_r
Cuts at: 0 1023 3642
Size: 1023 2619
EarI CTCTTCn'nnn_
Cuts at: 0 242 707 1817 2424 2696 3482 3542 3642
Size: 242 465 1110 607 272 786 60 100
Fragments arranged by size:
1110 786 607 465 272 242 100 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 1514 3268 3286 3642
Size: 34 1480 1754 18 356
Fragments arranged by size:
1754 1480 356 34 18
EcoICRI GAG'CTC
Cuts at: 0 2430 3642
Size: 2430 1212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 484 593 697 1460 1603 2316 2416
Size: 478 6 109 104 763 143 713 100
Cuts at: 2416 2843 3575 3642
Size: 427 732 67
Fragments arranged by size:
763 732 713 478 427 143 109 104
100 67 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2050 3642
Size: 2050 1592
EcoO109I rG'GnC_Cy
Cuts at: 0 2111 3642
Size: 2111 1531
EcoRI G'AATT_C
Cuts at: 0 905 3642
Size: 905 2737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 145 3642
Size: 145 3497
HaeII r_GCGC'y
Cuts at: 0 666 3189 3642
Size: 666 2523 453
Fragments arranged by size:
2523 666 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 1468 1500 1951 1983 2902 2932
Size: 532 32 904 32 451 32 919 30
Cuts at: 2932 2934 2964 3543 3575 3642
Size: 2 30 579 32 67
Fragments arranged by size:
919 904 579 532 451 67 32 32
32 32 30 30 2
HindIII A'AGCT_T
Cuts at: 0 127 1610 3642
Size: 127 1483 2032
Fragments arranged by size:
2032 1483 127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1568 2969 3642
Size: 1097 471 1401 673
Fragments arranged by size:
1401 1097 673 471
MslI CAynn'nnrTG
Cuts at: 0 914 1316 1556 1743 1793 2038 2246 2540
Size: 914 402 240 187 50 245 208 294
Cuts at: 2540 3081 3642
Size: 541 561
Fragments arranged by size:
914 561 541 402 294 245 240 208
187 50
MspA1I CmG'CkG
Cuts at: 0 2868 3642
Size: 2868 774
NcoI C'CATG_G
Cuts at: 0 3566 3642
Size: 3566 76
NdeI CA'TA_TG
Cuts at: 0 776 2801 3642
Size: 776 2025 841
Fragments arranged by size:
2025 841 776
NsiI A_TGCA'T
Cuts at: 0 3000 3642
Size: 3000 642
NspI r_CATG'y
Cuts at: 0 1968 2919 2994 3002 3642
Size: 1968 951 75 8 640
Fragments arranged by size:
1968 951 640 75 8
PciI A'CATG_T
Cuts at: 0 1964 2915 3642
Size: 1964 951 727
Fragments arranged by size:
1964 951 727
PfoI T'CCnGG_A
Cuts at: 0 2332 3642
Size: 2332 1310
PmlI CAC'GTG
Cuts at: 0 219 3642
Size: 219 3423
PpuMI rG'GwC_Cy
Cuts at: 0 2111 3642
Size: 2111 1531
PshAI GACnn'nnGTC
Cuts at: 0 2951 3642
Size: 2951 691
PspXI vC'TCGA_Gb
Cuts at: 0 766 3642
Size: 766 2876
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1370 1402 3316 3348 3642
Size: 1370 32 1914 32 294
Fragments arranged by size:
1914 1370 294 32 32
PstI C_TGCA'G
Cuts at: 0 1844 3642
Size: 1844 1798
SacI G_AGCT'C
Cuts at: 0 2432 3642
Size: 2432 1210
SapI GCTCTTCn'nnn_
Cuts at: 0 2696 3642
Size: 2696 946
ScaI AGT'ACT
Cuts at: 0 2098 3642
Size: 2098 1544
SfcI C'TryA_G
Cuts at: 0 317 735 972 1390 1840 2228 3642
Size: 317 418 237 418 450 388 1414
Fragments arranged by size:
1414 450 418 418 388 317 237
SmlI C'TyrA_G
Cuts at: 0 766 1638 3135 3642
Size: 766 872 1497 507
Fragments arranged by size:
1497 872 766 507
SnaBI TAC'GTA
Cuts at: 0 1678 3642
Size: 1678 1964
SpeI A'CTAG_T
Cuts at: 0 1234 3642
Size: 1234 2408
SspI AAT'ATT
Cuts at: 0 1521 2304 3642
Size: 1521 783 1338
Fragments arranged by size:
1521 1338 783
StuI AGG'CCT
Cuts at: 0 868 2924 3642
Size: 868 2056 718
Fragments arranged by size:
2056 868 718
StyI C'CwwG_G
Cuts at: 0 555 822 3566 3642
Size: 555 267 2744 76
Fragments arranged by size:
2744 555 267 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 1522 3642
Size: 775 747 2120
Fragments arranged by size:
2120 775 747
TatI w'GTAC_w
Cuts at: 0 1914 1971 2096 3642
Size: 1914 57 125 1546
Fragments arranged by size:
1914 1546 125 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1714 1746 2676 2708 3217 3249 3642
Size: 1714 32 930 32 509 32 393
Fragments arranged by size:
1714 930 509 393 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 1422 3642
Size: 453 969 2220
Fragments arranged by size:
2220 969 453
XhoI C'TCGA_G
Cuts at: 0 766 3642
Size: 766 2876
XmnI GAAnn'nnTTC
Cuts at: 0 952 1766 3103 3642
Size: 952 814 1337 539
Fragments arranged by size:
1337 952 814 539
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AhdI AleI
AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BfrBI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII
MmeI MslI MspA1I NcoI NdeI NsiI NspI PciI
PfoI PmlI PpuMI PshAI PspXI PsrI PstI SacI
SapI ScaI SfcI SmlI SnaBI SpeI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BclI BglI
BglII BmgBI BmtI BsaHI BsgI BsiWI BspEI BsrFI
BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI DrdI
EagI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PflMI PmeI PpiI PsiI
PspOMI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI Tth111I XbaI XmaI ZraI