Restriction map for
pF1KA0563
(Linear) (Six-base) MAPSORT of: KIAA0563.seq Check: 4448 from: 1 to: 4902
KIAA0563.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 3692 4002 4902
Size: 3692 310 900
Fragments arranged by size:
3692 900 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 911 2090 2384 3194 4118 4902
Size: 469 442 1179 294 810 924 784
Fragments arranged by size:
1179 924 810 784 469 442 294
AflII C'TTAA_G
Cuts at: 0 3013 4083 4902
Size: 3013 1070 819
Fragments arranged by size:
3013 1070 819
AhdI GACnn_n'nnGTC
Cuts at: 0 699 1148 4902
Size: 699 449 3754
Fragments arranged by size:
3754 699 449
AleI CACnn'nnGTG
Cuts at: 0 27 4902
Size: 27 4875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1508 4902
Size: 1508 3394
AlwNI CAG_nnn'CTG
Cuts at: 0 117 2287 2346 3489 3736 4440 4902
Size: 117 2170 59 1143 247 704 462
Fragments arranged by size:
2170 1143 704 462 247 117 59
ApaI G_GGCC'C
Cuts at: 0 61 266 626 1375 3511 4524 4902
Size: 61 205 360 749 2136 1013 378
Fragments arranged by size:
2136 1013 749 378 360 205 61
ApoI r'AATT_y
Cuts at: 0 312 2610 4053 4421 4902
Size: 312 2298 1443 368 481
Fragments arranged by size:
2298 1443 481 368 312
AscI GG'CGCG_CC
Cuts at: 0 3761 4902
Size: 3761 1141
AseI AT'TA_AT
Cuts at: 0 1457 4655 4902
Size: 1457 3198 247
Fragments arranged by size:
3198 1457 247
AvaI C'yCGr_G
Cuts at: 0 1976 4366 4902
Size: 1976 2390 536
Fragments arranged by size:
2390 1976 536
BanI G'GyrC_C
Cuts at: 0 2593 3574 4902
Size: 2593 981 1328
Fragments arranged by size:
2593 1328 981
BanII G_rGCy'C
Cuts at: 0 61 176 266 626 665 1375 2498 2579
Size: 61 115 90 360 39 710 1123 81
Cuts at: 2579 3275 3511 4373 4524 4902
Size: 696 236 862 151 378
Fragments arranged by size:
1123 862 710 696 378 360 236 151
115 90 81 61 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 698 1097 1523 2317 3883 4645 4902
Size: 173 525 399 426 794 1566 762 257
Fragments arranged by size:
1566 794 762 525 426 399 257 173
BbvCI CC'TCA_GC
Cuts at: 0 1517 4902
Size: 1517 3385
BciVI GTATCCnnnnn_n'
Cuts at: 0 581 4617 4902
Size: 581 4036 285
Fragments arranged by size:
4036 581 285
BclI T'GATC_A
Cuts at: 0 252 4410 4902
Size: 252 4158 492
Fragments arranged by size:
4158 492 252
BglI GCCn_nnn'nGGC
Cuts at: 0 621 4902
Size: 621 4281
BglII A'GATC_T
Cuts at: 0 4808 4902
Size: 4808 94
BlpI GC'TnA_GC
Cuts at: 0 110 464 1499 1793 2123 4902
Size: 110 354 1035 294 330 2779
Fragments arranged by size:
2779 1035 354 330 294 110
Bme1580I G_kGCm'C
Cuts at: 0 19 61 266 626 1375 3511 3579 4354
Size: 19 42 205 360 749 2136 68 775
Cuts at: 4354 4524 4902
Size: 170 378
Fragments arranged by size:
2136 775 749 378 360 205 170 68
42 19
BmgBI CAC'GTC
Cuts at: 0 1248 4902
Size: 1248 3654
BmrI ACTGGGnnnn_n'
Cuts at: 0 1217 4902
Size: 1217 3685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 836 1488 2247 3291 4902
Size: 836 652 759 1044 1611
Fragments arranged by size:
1611 1044 836 759 652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 139 368 382 572 602 697 779
Size: 124 15 229 14 190 30 95 82
Cuts at: 779 836 1022 1142 1172 1226 1550 1583 1646
Size: 57 186 120 30 54 324 33 63
Cuts at: 1646 1838 2060 2188 2326 2728 3759 4539 4902
Size: 192 222 128 138 402 1031 780 363
Fragments arranged by size:
1031 780 402 363 324 229 222 192
190 186 138 128 124 120 95 82
63 57 54 33 30 30 15 14
Bpu10I CC'TnA_GC
Cuts at: 0 170 1517 2399 4902
Size: 170 1347 882 2503
Fragments arranged by size:
2503 1347 882 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1604 2126 4085 4720 4839 4902
Size: 769 835 522 1959 635 119 63
Fragments arranged by size:
1959 835 769 635 522 119 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 964 1135 1699 2221 4082 4902
Size: 676 288 171 564 522 1861 820
Fragments arranged by size:
1861 820 676 564 522 288 171
BsaBI GATnn'nnATC
Cuts at: 0 2945 4902
Size: 2945 1957
BsaWI w'CCGG_w
Cuts at: 0 1446 4493 4902
Size: 1446 3047 409
Fragments arranged by size:
3047 1446 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1513 1543 1644 1674 1704 1734 1836 1866
Size: 1513 30 101 30 30 30 102 30
Cuts at: 1866 2001 2031 2052 2055 2082 2085 2304 2334
Size: 135 30 21 3 27 3 219 30
Cuts at: 2334 2455 2485 4902
Size: 121 30 2417
Fragments arranged by size:
2417 1513 219 135 121 102 101 30
30 30 30 30 30 30 30 27
21 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 180 1006 1534 2060 2063 2239 2312 3283
Size: 180 826 528 526 3 176 73 971
Cuts at: 3283 4894 4897 4902
Size: 1611 3 5
Fragments arranged by size:
1611 971 826 528 526 180 176 73
5 3 3
BseYI C'CCAG_C
Cuts at: 0 231 726 783 962 1133 1481 1554 1810
Size: 231 495 57 179 171 348 73 256
Cuts at: 1810 4282 4433 4902
Size: 2472 151 469
Fragments arranged by size:
2472 495 469 348 256 231 179 171
151 73 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1298 2434 2435 4902
Size: 1270 28 1136 1 2467
Fragments arranged by size:
2467 1270 1136 28 1
BsiHKAI G_wGCw'C
Cuts at: 0 2498 4666 4902
Size: 2498 2168 236
Fragments arranged by size:
2498 2168 236
BsmI GAATG_Cn'
Cuts at: 0 1605 1962 2127 2891 4291 4902
Size: 1605 357 165 764 1400 611
Fragments arranged by size:
1605 1400 764 611 357 165
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4557 4902
Size: 4557 345
Bsp1286I G_dGCh'C
Cuts at: 0 19 61 176 266 626 665 1375 2498
Size: 19 42 115 90 360 39 710 1123
Cuts at: 2498 2579 3275 3511 3579 4354 4373 4524 4666
Size: 81 696 236 68 775 19 151 142
Cuts at: 4666 4902
Size: 236
Fragments arranged by size:
1123 775 710 696 360 236 236 151
142 115 90 81 68 42 39 19
19
BspEI T'CCGG_A
Cuts at: 0 1446 4493 4902
Size: 1446 3047 409
Fragments arranged by size:
3047 1446 409
BspHI T'CATG_A
Cuts at: 0 831 1167 3026 3209 4902
Size: 831 336 1859 183 1693
Fragments arranged by size:
1859 1693 831 336 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 909 2403 2512 4902
Size: 909 1494 109 2390
Fragments arranged by size:
2390 1494 909 109
BsrBI CCG'CTC
Cuts at: 0 10 138 4902
Size: 10 128 4764
Fragments arranged by size:
4764 128 10
BsrDI GCAATG_nn'
Cuts at: 0 2844 3132 4390 4478 4902
Size: 2844 288 1258 88 424
Fragments arranged by size:
2844 1258 424 288 88
BsrFI r'CCGG_y
Cuts at: 0 3479 4902
Size: 3479 1423
BssHII G'CGCG_C
Cuts at: 0 3761 4902
Size: 3761 1141
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2886 4183 4902
Size: 2886 1297 719
Fragments arranged by size:
2886 1297 719
BstEII G'GTnAC_C
Cuts at: 0 915 1192 4902
Size: 915 277 3710
Fragments arranged by size:
3710 915 277
BstYI r'GATC_y
Cuts at: 0 456 1641 2940 4808 4902
Size: 456 1185 1299 1868 94
Fragments arranged by size:
1868 1299 1185 456 94
Bsu36I CC'TnA_GG
Cuts at: 0 1922 2087 4268 4902
Size: 1922 165 2181 634
Fragments arranged by size:
2181 1922 634 165
BtgI C'CryG_G
Cuts at: 0 61 202 241 2219 4902
Size: 61 141 39 1978 2683
Fragments arranged by size:
2683 1978 141 61 39
BtsI GCAGTG_nn'
Cuts at: 0 3291 3596 4902
Size: 3291 305 1306
Fragments arranged by size:
3291 1306 305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 70 105 4902
Size: 70 35 4797
Fragments arranged by size:
4797 70 35
DraI TTT'AAA
Cuts at: 0 398 2955 3093 4902
Size: 398 2557 138 1809
Fragments arranged by size:
2557 1809 398 138
DraIII CAC_nnn'GTG
Cuts at: 0 1225 4902
Size: 1225 3677
EarI CTCTTCn'nnn_
Cuts at: 0 711 1888 4036 4902
Size: 711 1177 2148 866
Fragments arranged by size:
2148 1177 866 711
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2545 4902
Size: 2545 2357
EcoICRI GAG'CTC
Cuts at: 0 2496 4902
Size: 2496 2406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 139 368 382 469 572 602 697
Size: 124 15 229 14 87 103 30 95
Cuts at: 697 779 836 911 1022 1142 1172 1226 1550
Size: 82 57 75 111 120 30 54 324
Cuts at: 1550 1583 1646 1838 2060 2090 2188 2326 2384
Size: 33 63 192 222 30 98 138 58
Cuts at: 2384 2728 3194 3759 4118 4539 4902
Size: 344 466 565 359 421 363
Fragments arranged by size:
565 466 421 363 359 344 324 229
222 192 138 124 120 111 103 98
95 87 82 75 63 58 57 54
33 30 30 30 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2075 3796 4064 4902
Size: 2075 1721 268 838
Fragments arranged by size:
2075 1721 838 268
EcoO109I rG'GnC_Cy
Cuts at: 0 57 132 262 263 622 623 780 1023
Size: 57 75 130 1 359 1 157 243
Cuts at: 1023 1371 1551 3664 4520 4902
Size: 348 180 2113 856 382
Fragments arranged by size:
2113 856 382 359 348 243 180 157
130 75 57 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 163 195 685 717 934 966 1105
Size: 26 137 32 490 32 217 32 139
Cuts at: 1105 1134 1137 1166 1513 1545 1698 1730 1832
Size: 29 3 29 347 32 153 32 102
Cuts at: 1832 1864 2052 2084 2220 2246 2252 2278 2304
Size: 32 188 32 136 26 6 26 26
Cuts at: 2304 2334 2336 2366 2506 2538 3388 3420 3995
Size: 30 2 30 140 32 850 32 575
Cuts at: 3995 4027 4800 4832 4902
Size: 32 773 32 70
Fragments arranged by size:
850 773 575 490 347 217 188 153
140 139 137 136 102 70 32 32
32 32 32 32 32 32 32 32
32 30 30 29 29 26 26 26
26 6 3 2
HincII GTy'rAC
Cuts at: 0 3390 4902
Size: 3390 1512
HindIII A'AGCT_T
Cuts at: 0 866 4377 4576 4902
Size: 866 3511 199 326
Fragments arranged by size:
3511 866 326 199
HpaI GTT'AAC
Cuts at: 0 3390 4902
Size: 3390 1512
MfeI C'AATT_G
Cuts at: 0 1028 4902
Size: 1028 3874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 618 1153 1501 1717 1795 1854 2239
Size: 451 167 535 348 216 78 59 385
Cuts at: 2239 2647 4902
Size: 408 2255
Fragments arranged by size:
2255 535 451 408 385 348 216 167
78 59
MslI CAynn'nnrTG
Cuts at: 0 27 3025 3214 4219 4902
Size: 27 2998 189 1005 683
Fragments arranged by size:
2998 1005 683 189 27
MspA1I CmG'CkG
Cuts at: 0 45 144 346 1498 1516 4282 4440 4902
Size: 45 99 202 1152 18 2766 158 462
Fragments arranged by size:
2766 1152 462 202 158 99 45 18
NcoI C'CATG_G
Cuts at: 0 61 4902
Size: 61 4841
NdeI CA'TA_TG
Cuts at: 0 2488 3068 4902
Size: 2488 580 1834
Fragments arranged by size:
2488 1834 580
NspI r_CATG'y
Cuts at: 0 3149 4224 4902
Size: 3149 1075 678
Fragments arranged by size:
3149 1075 678
PasI CC'CwG_GG
Cuts at: 0 159 4902
Size: 159 4743
PflMI CCAn_nnn'nTGG
Cuts at: 0 4726 4902
Size: 4726 176
PfoI T'CCnGG_A
Cuts at: 0 326 2076 4555 4719 4902
Size: 326 1750 2479 164 183
Fragments arranged by size:
2479 1750 326 183 164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1970 2002 4902
Size: 1970 32 2900
Fragments arranged by size:
2900 1970 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 4902
Size: 132 4770
PshAI GACnn'nnGTC
Cuts at: 0 2114 2365 4094 4902
Size: 2114 251 1729 808
Fragments arranged by size:
2114 1729 808 251
PsiI TTA'TAA
Cuts at: 0 2824 4902
Size: 2824 2078
PspOMI G'GGCC_C
Cuts at: 0 57 262 622 1371 3507 4520 4902
Size: 57 205 360 749 2136 1013 382
Fragments arranged by size:
2136 1013 749 382 360 205 57
PspXI vC'TCGA_Gb
Cuts at: 0 1976 4902
Size: 1976 2926
PstI C_TGCA'G
Cuts at: 0 906 1497 4038 4445 4902
Size: 906 591 2541 407 457
Fragments arranged by size:
2541 906 591 457 407
PvuII CAG'CTG
Cuts at: 0 144 346 1498 4440 4902
Size: 144 202 1152 2942 462
Fragments arranged by size:
2942 1152 462 202 144
SacI G_AGCT'C
Cuts at: 0 2498 4902
Size: 2498 2404
SapI GCTCTTCn'nnn_
Cuts at: 0 4036 4902
Size: 4036 866
SexAI A'CCwGG_T
Cuts at: 0 1776 1946 4902
Size: 1776 170 2956
Fragments arranged by size:
2956 1776 170
SfcI C'TryA_G
Cuts at: 0 902 1493 1901 2048 2063 2195 2279 2297
Size: 902 591 408 147 15 132 84 18
Cuts at: 2297 2315 3185 3467 4034 4441 4902
Size: 18 870 282 567 407 461
Fragments arranged by size:
902 870 591 567 461 408 407 282
147 132 84 18 18 15
SmlI C'TyrA_G
Cuts at: 0 748 1619 1976 2141 3013 4083 4100 4699
Size: 748 871 357 165 872 1070 17 599
Cuts at: 4699 4854 4902
Size: 155 48
Fragments arranged by size:
1070 872 871 748 599 357 165 155
48 17
SpeI A'CTAG_T
Cuts at: 0 96 4902
Size: 96 4806
SspI AAT'ATT
Cuts at: 0 550 4902
Size: 550 4352
StuI AGG'CCT
Cuts at: 0 2470 2732 2875 4902
Size: 2470 262 143 2027
Fragments arranged by size:
2470 2027 262 143
StyI C'CwwG_G
Cuts at: 0 61 2616 3511 3631 3755 3956 4902
Size: 61 2555 895 120 124 201 946
Fragments arranged by size:
2555 946 895 201 124 120 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1236 1824 2446 2599 2686 3727 3969 4902
Size: 1236 588 622 153 87 1041 242 933
Fragments arranged by size:
1236 1041 933 622 588 242 153 87
TatI w'GTAC_w
Cuts at: 0 556 3352 4902
Size: 556 2796 1550
Fragments arranged by size:
2796 1550 556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 493 525 2455 2487 3153 3185 3820 3852
Size: 493 32 1930 32 666 32 635 32
Cuts at: 3852 4902
Size: 1050
Fragments arranged by size:
1930 1050 666 635 493 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 657 2976 4902
Size: 657 2319 1926
Fragments arranged by size:
2319 1926 657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 239 467 4902
Size: 239 228 4435
Fragments arranged by size:
4435 239 228
XhoI C'TCGA_G
Cuts at: 0 1976 4902
Size: 1976 2926
XmnI GAAnn'nnTTC
Cuts at: 0 3748 4902
Size: 3748 1154
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI AlwNI ApaI
ApoI AscI AseI AvaI BanI BanII BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII
BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NcoI NdeI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuII SacI SapI SexAI SfcI SmlI
SpeI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AloI
ApaLI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI
BmtI BsaAI BsaHI BsiEI BsiWI BsrGI BssSI BstBI
BstXI BstZ17I BtgZI ClaI DrdI EaeI EagI EcoRI
EcoRV FalI FseI FspI FspAI HaeII KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI Tth111I XmaI
ZraI