Restriction map for
pF1KA0555
(Linear) (Six-base) MAPSORT of: KIAA0555.seq Check: 6799 from: 1 to: 2397
KIAA0555.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 483 796 2397
Size: 483 313 1601
Fragments arranged by size:
1601 483 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1132 1165 1433 2230 2397
Size: 700 432 33 268 797 167
Fragments arranged by size:
797 700 432 268 167 33
AfeI AGC'GCT
Cuts at: 0 395 2397
Size: 395 2002
AflII C'TTAA_G
Cuts at: 0 448 2397
Size: 448 1949
AflIII A'CryG_T
Cuts at: 0 942 2397
Size: 942 1455
AgeI A'CCGG_T
Cuts at: 0 1640 2397
Size: 1640 757
ApoI r'AATT_y
Cuts at: 0 791 1071 1748 1866 2285 2397
Size: 791 280 677 118 419 112
Fragments arranged by size:
791 677 419 280 118 112
AvaI C'yCGr_G
Cuts at: 0 34 410 2397
Size: 34 376 1987
Fragments arranged by size:
1987 376 34
BamHI G'GATC_C
Cuts at: 0 1934 2397
Size: 1934 463
BanII G_rGCy'C
Cuts at: 0 1217 2264 2397
Size: 1217 1047 133
Fragments arranged by size:
1217 1047 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 157 902 1586 2397
Size: 77 80 745 684 811
Fragments arranged by size:
811 745 684 80 77
BbvCI CC'TCA_GC
Cuts at: 0 1955 2176 2212 2397
Size: 1955 221 36 185
Fragments arranged by size:
1955 221 185 36
BglII A'GATC_T
Cuts at: 0 1784 2397
Size: 1784 613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1252 2397
Size: 1252 1145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 700 1708 2050 2397
Size: 202 498 1008 342 347
Fragments arranged by size:
1008 498 347 342 202
Bpu10I CC'TnA_GC
Cuts at: 0 1558 1955 2176 2212 2397
Size: 1558 397 221 36 185
Fragments arranged by size:
1558 397 221 185 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 325 1517 2397
Size: 140 185 1192 880
Fragments arranged by size:
1192 880 185 140
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 1664 2397
Size: 676 988 733
Fragments arranged by size:
988 733 676
BsaAI yAC'GTr
Cuts at: 0 319 2397
Size: 319 2078
BsaHI Gr'CG_yC
Cuts at: 0 1664 2397
Size: 1664 733
BsaWI w'CCGG_w
Cuts at: 0 1640 2397
Size: 1640 757
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 692 722 748 778 2397
Size: 692 30 26 30 1619
Fragments arranged by size:
1619 692 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 430 2185 2397
Size: 250 180 1755 212
Fragments arranged by size:
1755 250 212 180
BseYI C'CCAG_C
Cuts at: 0 541 2397
Size: 541 1856
BsiHKAI G_wGCw'C
Cuts at: 0 360 1217 1779 2264 2397
Size: 360 857 562 485 133
Fragments arranged by size:
857 562 485 360 133
BsmI GAATG_Cn'
Cuts at: 0 879 2397
Size: 879 1518
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1716 2397
Size: 1716 681
Bsp1286I G_dGCh'C
Cuts at: 0 360 1217 1779 2264 2397
Size: 360 857 562 485 133
Fragments arranged by size:
857 562 485 360 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 796 2397
Size: 483 313 1601
Fragments arranged by size:
1601 483 313
BsrBI CCG'CTC
Cuts at: 0 609 2397
Size: 609 1788
BsrDI GCAATG_nn'
Cuts at: 0 970 2397
Size: 970 1427
BsrFI r'CCGG_y
Cuts at: 0 1640 2397
Size: 1640 757
BsrGI T'GTAC_A
Cuts at: 0 699 2105 2397
Size: 699 1406 292
Fragments arranged by size:
1406 699 292
BssSI C'ACGA_G
Cuts at: 0 286 2397
Size: 286 2111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 2397
Size: 201 2196
BstBI TT'CG_AA
Cuts at: 0 1695 2397
Size: 1695 702
BstYI r'GATC_y
Cuts at: 0 329 1784 1934 2397
Size: 329 1455 150 463
Fragments arranged by size:
1455 463 329 150
Bsu36I CC'TnA_GG
Cuts at: 0 76 2397
Size: 76 2321
BtgI C'CryG_G
Cuts at: 0 1343 2397
Size: 1343 1054
BtsI GCAGTG_nn'
Cuts at: 0 381 822 2397
Size: 381 441 1575
Fragments arranged by size:
1575 441 381
DraIII CAC_nnn'GTG
Cuts at: 0 201 2397
Size: 201 2196
EaeI y'GGCC_r
Cuts at: 0 482 1889 2397
Size: 482 1407 508
Fragments arranged by size:
1407 508 482
EarI CTCTTCn'nnn_
Cuts at: 0 160 1706 2212 2397
Size: 160 1546 506 185
Fragments arranged by size:
1546 506 185 160
EcoICRI GAG'CTC
Cuts at: 0 1215 2262 2397
Size: 1215 1047 135
Fragments arranged by size:
1215 1047 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 700 1132 1165 1433 1708 2050 2230
Size: 202 498 432 33 268 275 342 180
Cuts at: 2230 2397
Size: 167
Fragments arranged by size:
498 432 342 275 268 202 180 167
33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 670 1483 2397
Size: 670 813 914
Fragments arranged by size:
914 813 670
EcoO109I rG'GnC_Cy
Cuts at: 0 546 1458 2397
Size: 546 912 939
Fragments arranged by size:
939 912 546
EcoRI G'AATT_C
Cuts at: 0 2285 2397
Size: 2285 112
EcoRV GAT'ATC
Cuts at: 0 1848 2397
Size: 1848 549
HaeII r_GCGC'y
Cuts at: 0 397 447 2397
Size: 397 50 1950
Fragments arranged by size:
1950 397 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 546 550 578 582 1303 1324 1335 1356
Size: 546 4 28 4 721 21 11 21
Cuts at: 1356 1490 1522 1584 1616 1711 1743 2397
Size: 134 32 62 32 95 32 654
Fragments arranged by size:
721 654 546 134 95 62 32 32
32 28 21 21 11 4 4
HindIII A'AGCT_T
Cuts at: 0 136 2397
Size: 136 2261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 735 2397
Size: 584 151 1662
Fragments arranged by size:
1662 584 151
MscI TGG'CCA
Cuts at: 0 1891 2397
Size: 1891 506
MspA1I CmG'CkG
Cuts at: 0 1411 2397
Size: 1411 986
NcoI C'CATG_G
Cuts at: 0 1343 2397
Size: 1343 1054
NdeI CA'TA_TG
Cuts at: 0 1473 2397
Size: 1473 924
PfoI T'CCnGG_A
Cuts at: 0 795 977 2084 2397
Size: 795 182 1107 313
Fragments arranged by size:
1107 795 313 182
PpuMI rG'GwC_Cy
Cuts at: 0 546 2397
Size: 546 1851
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1359 1391 2397
Size: 1359 32 1006
Fragments arranged by size:
1359 1006 32
PstI C_TGCA'G
Cuts at: 0 257 829 2397
Size: 257 572 1568
Fragments arranged by size:
1568 572 257
PvuII CAG'CTG
Cuts at: 0 1411 2397
Size: 1411 986
SacI G_AGCT'C
Cuts at: 0 1217 2264 2397
Size: 1217 1047 133
Fragments arranged by size:
1217 1047 133
SapI GCTCTTCn'nnn_
Cuts at: 0 2212 2397
Size: 2212 185
SfcI C'TryA_G
Cuts at: 0 253 825 1483 1657 1780 2046 2116 2397
Size: 253 572 658 174 123 266 70 281
Fragments arranged by size:
658 572 281 266 253 174 123 70
SmaI CCC'GGG
Cuts at: 0 412 2397
Size: 412 1985
SmlI C'TyrA_G
Cuts at: 0 155 340 448 1532 2397
Size: 155 185 108 1084 865
Fragments arranged by size:
1084 865 185 155 108
StuI AGG'CCT
Cuts at: 0 1284 2397
Size: 1284 1113
StyI C'CwwG_G
Cuts at: 0 125 644 1064 1343 1526 2397
Size: 125 519 420 279 183 871
Fragments arranged by size:
871 519 420 279 183 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1241 1769 1843 2397
Size: 1241 528 74 554
Fragments arranged by size:
1241 554 528 74
TatI w'GTAC_w
Cuts at: 0 699 2105 2397
Size: 699 1406 292
Fragments arranged by size:
1406 699 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 748 780 2397
Size: 748 32 1617
Fragments arranged by size:
1617 748 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 2397
Size: 492 1905
XmaI C'CCGG_G
Cuts at: 0 410 2397
Size: 410 1987
XmnI GAAnn'nnTTC
Cuts at: 0 1263 2348 2397
Size: 1263 1085 49
Fragments arranged by size:
1263 1085 49
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AgeI ApoI AvaI
BamHI BanII BbsI BbvCI BglII BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HindIII MmeI MscI MspA1I NcoI
NdeI PfoI PpuMI PsrI PstI PvuII SacI SapI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BsaBI BsgI BsiEI BsiWI
BspEI BspHI BssHII BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI