Restriction map for
pF1KA0553
(Linear) (Six-base) MAPSORT of: KIAA0553.seq Check: 2144 from: 1 to: 4272
KIAA0553.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1671 4272
Size: 1671 2601
AccI GT'mk_AC
Cuts at: 0 2418 4272
Size: 2418 1854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 292 770 1652 2069 2381 3032 3332
Size: 59 233 478 882 417 312 651 300
Cuts at: 3332 4272
Size: 940
Fragments arranged by size:
940 882 651 478 417 312 300 233
59
AfeI AGC'GCT
Cuts at: 0 2485 2623 4272
Size: 2485 138 1649
Fragments arranged by size:
2485 1649 138
AleI CACnn'nnGTG
Cuts at: 0 2126 4272
Size: 2126 2146
AlwNI CAG_nnn'CTG
Cuts at: 0 430 937 1675 1945 3556 3771 3868 3928
Size: 430 507 738 270 1611 215 97 60
Cuts at: 3928 4189 4245 4272
Size: 261 56 27
Fragments arranged by size:
1611 738 507 430 270 261 215 97
60 56 27
ApaI G_GGCC'C
Cuts at: 0 3128 3164 3308 4118 4272
Size: 3128 36 144 810 154
Fragments arranged by size:
3128 810 154 144 36
ApaLI G'TGCA_C
Cuts at: 0 389 4272
Size: 389 3883
ApoI r'AATT_y
Cuts at: 0 214 520 4272
Size: 214 306 3752
Fragments arranged by size:
3752 306 214
AseI AT'TA_AT
Cuts at: 0 1432 4272
Size: 1432 2840
AvaI C'yCGr_G
Cuts at: 0 2633 4272
Size: 2633 1639
BamHI G'GATC_C
Cuts at: 0 3627 4272
Size: 3627 645
BanI G'GyrC_C
Cuts at: 0 56 490 2164 2339 3439 4272
Size: 56 434 1674 175 1100 833
Fragments arranged by size:
1674 1100 833 434 175 56
BanII G_rGCy'C
Cuts at: 0 1184 2016 2761 2809 3128 3164 3308 4118
Size: 1184 832 745 48 319 36 144 810
Cuts at: 4118 4272
Size: 154
Fragments arranged by size:
1184 832 810 745 319 154 144 48
36
BbeI G_GCGC'C
Cuts at: 0 60 2343 4272
Size: 60 2283 1929
Fragments arranged by size:
2283 1929 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 161 857 1739 3020 4272
Size: 89 72 696 882 1281 1252
Fragments arranged by size:
1281 1252 882 696 89 72
BbvCI CC'TCA_GC
Cuts at: 0 1205 3656 3801 4098 4272
Size: 1205 2451 145 297 174
Fragments arranged by size:
2451 1205 297 174 145
BclI T'GATC_A
Cuts at: 0 240 1239 3417 4272
Size: 240 999 2178 855
Fragments arranged by size:
2178 999 855 240
BglI GCCn_nnn'nGGC
Cuts at: 0 685 4272
Size: 685 3587
BglII A'GATC_T
Cuts at: 0 267 2852 4272
Size: 267 2585 1420
Fragments arranged by size:
2585 1420 267
BlpI GC'TnA_GC
Cuts at: 0 3746 4272
Size: 3746 526
Bme1580I G_kGCm'C
Cuts at: 0 393 3128 3164 3308 4118 4272
Size: 393 2735 36 144 810 154
Fragments arranged by size:
2735 810 393 154 144 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1375 2198 2262 2859 3500 3599 3614 4272
Size: 1375 823 64 597 641 99 15 658
Fragments arranged by size:
1375 823 658 641 597 99 64 15
BmtI G_CTAG'C
Cuts at: 0 4073 4272
Size: 4073 199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1314 1878 4272
Size: 1314 564 2394
Fragments arranged by size:
2394 1314 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 2763 3067 3422 3544 3718 4272
Size: 1396 1367 304 355 122 174 554
Fragments arranged by size:
1396 1367 554 355 304 174 122
Bpu10I CC'TnA_GC
Cuts at: 0 1205 3656 3801 4098 4272
Size: 1205 2451 145 297 174
Fragments arranged by size:
2451 1205 297 174 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2000 2310 2361 2610 3118 3613 4272
Size: 2000 310 51 249 508 495 659
Fragments arranged by size:
2000 659 508 495 310 249 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 1270 4272
Size: 1270 3002
BsaHI Gr'CG_yC
Cuts at: 0 57 2340 2829 4272
Size: 57 2283 489 1443
Fragments arranged by size:
2283 1443 489 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1217 1247 1968 1998 2673 2703 4272
Size: 1217 30 721 30 675 30 1569
Fragments arranged by size:
1569 1217 721 675 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1548 2827 2942 3109 3724 4272
Size: 1548 1279 115 167 615 548
Fragments arranged by size:
1548 1279 615 548 167 115
BseYI C'CCAG_C
Cuts at: 0 922 960 1326 1753 1935 2027 2043 3121
Size: 922 38 366 427 182 92 16 1078
Cuts at: 3121 3219 3444 3816 3992 4258 4272
Size: 98 225 372 176 266 14
Fragments arranged by size:
1078 922 427 372 366 266 225 182
176 98 92 38 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2233 3121 4181 4272
Size: 2233 888 1060 91
Fragments arranged by size:
2233 1060 888 91
BsiHKAI G_wGCw'C
Cuts at: 0 393 2016 4272
Size: 393 1623 2256
Fragments arranged by size:
2256 1623 393
BsmI GAATG_Cn'
Cuts at: 0 1059 2310 2820 3941 4272
Size: 1059 1251 510 1121 331
Fragments arranged by size:
1251 1121 1059 510 331
Bsp1286I G_dGCh'C
Cuts at: 0 393 1184 2016 2761 2809 3128 3164 3308
Size: 393 791 832 745 48 319 36 144
Cuts at: 3308 4118 4272
Size: 810 154
Fragments arranged by size:
832 810 791 745 393 319 154 144
48 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1671 4253 4272
Size: 1671 2582 19
Fragments arranged by size:
2582 1671 19
BsrBI CCG'CTC
Cuts at: 0 2324 2861 4272
Size: 2324 537 1411
Fragments arranged by size:
2324 1411 537
BsrDI GCAATG_nn'
Cuts at: 0 646 4272
Size: 646 3626
BsrFI r'CCGG_y
Cuts at: 0 1784 2180 2243 2336 4272
Size: 1784 396 63 93 1936
Fragments arranged by size:
1936 1784 396 93 63
BssSI C'ACGA_G
Cuts at: 0 2800 4272
Size: 2800 1472
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3928 4189 4272
Size: 3928 261 83
Fragments arranged by size:
3928 261 83
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 579 592 2107 4272
Size: 150 429 13 1515 2165
Fragments arranged by size:
2165 1515 429 150 13
BstYI r'GATC_y
Cuts at: 0 267 307 514 1584 2035 2629 2852 3053
Size: 267 40 207 1070 451 594 223 201
Cuts at: 3053 3627 4272
Size: 574 645
Fragments arranged by size:
1070 645 594 574 451 267 223 207
201 40
Bsu36I CC'TnA_GG
Cuts at: 0 857 1221 1641 2810 3707 4272
Size: 857 364 420 1169 897 565
Fragments arranged by size:
1169 897 857 565 420 364
BtgI C'CryG_G
Cuts at: 0 1716 2106 4171 4263 4272
Size: 1716 390 2065 92 9
Fragments arranged by size:
2065 1716 390 92 9
BtsI GCAGTG_nn'
Cuts at: 0 494 548 966 1403 2067 2310 3029 4193
Size: 494 54 418 437 664 243 719 1164
Cuts at: 4193 4272
Size: 79
Fragments arranged by size:
1164 719 664 494 437 418 243 79
54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 407 442 3050 3085 4146 4181 4272
Size: 407 35 2608 35 1061 35 91
Fragments arranged by size:
2608 1061 407 91 35 35 35
DraI TTT'AAA
Cuts at: 0 1494 4272
Size: 1494 2778
DraIII CAC_nnn'GTG
Cuts at: 0 3426 4272
Size: 3426 846
EaeI y'GGCC_r
Cuts at: 0 1319 1417 2334 3773 4047 4272
Size: 1319 98 917 1439 274 225
Fragments arranged by size:
1439 1319 917 274 225 98
EarI CTCTTCn'nnn_
Cuts at: 0 86 306 769 1305 1847 2045 2151 2282
Size: 86 220 463 536 542 198 106 131
Cuts at: 2282 2400 2779 2979 3245 3269 3293 3383 4272
Size: 118 379 200 266 24 24 90 889
Fragments arranged by size:
889 542 536 463 379 266 220 200
198 131 118 106 90 86 24 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1634 2022 4272
Size: 1634 388 2250
Fragments arranged by size:
2250 1634 388
EcoICRI GAG'CTC
Cuts at: 0 2014 4272
Size: 2014 2258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 292 770 1396 1652 2069 2381 2763
Size: 59 233 478 626 256 417 312 382
Cuts at: 2763 3032 3067 3332 3422 3544 3718 4272
Size: 269 35 265 90 122 174 554
Fragments arranged by size:
626 554 478 417 382 312 269 265
256 233 174 122 90 59 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1667 4272
Size: 1667 2605
EcoO109I rG'GnC_Cy
Cuts at: 0 541 641 743 869 1351 1954 2902 3138
Size: 541 100 102 126 482 603 948 236
Cuts at: 3138 3304 3305 3471 3553 4115 4242 4272
Size: 166 1 166 82 562 127 30
Fragments arranged by size:
948 603 562 541 482 236 166 166
127 126 102 100 82 30 1
EcoRI G'AATT_C
Cuts at: 0 520 4272
Size: 520 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1236 2149 4272
Size: 1236 913 2123
Fragments arranged by size:
2123 1236 913
HaeII r_GCGC'y
Cuts at: 0 60 2343 2487 2625 4272
Size: 60 2283 144 138 1647
Fragments arranged by size:
2283 1647 144 138 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 810 842 853 885 1968 2000 2221 2253
Size: 810 32 11 32 1083 32 221 32
Cuts at: 2253 3007 3039 3382 3414 4272
Size: 754 32 343 32 858
Fragments arranged by size:
1083 858 810 754 343 221 32 32
32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 123 3644 4272
Size: 123 3521 628
Fragments arranged by size:
3521 628 123
KasI G'GCGC_C
Cuts at: 0 56 2339 4272
Size: 56 2283 1933
Fragments arranged by size:
2283 1933 56
MfeI C'AATT_G
Cuts at: 0 137 4272
Size: 137 4135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 4272
Size: 1319 2953
MscI TGG'CCA
Cuts at: 0 1321 1419 3775 4049 4272
Size: 1321 98 2356 274 223
Fragments arranged by size:
2356 1321 274 223 98
MslI CAynn'nnrTG
Cuts at: 0 19 2126 4272
Size: 19 2107 2146
Fragments arranged by size:
2146 2107 19
MspA1I CmG'CkG
Cuts at: 0 2031 2047 2708 3805 3925 4189 4272
Size: 2031 16 661 1097 120 264 83
Fragments arranged by size:
2031 1097 661 264 120 83 16
NaeI GCC'GGC
Cuts at: 0 2338 4272
Size: 2338 1934
NarI GG'CG_CC
Cuts at: 0 57 2340 4272
Size: 57 2283 1932
Fragments arranged by size:
2283 1932 57
NcoI C'CATG_G
Cuts at: 0 1716 2106 4263 4272
Size: 1716 390 2157 9
Fragments arranged by size:
2157 1716 390 9
NgoMIV G'CCGG_C
Cuts at: 0 2336 4272
Size: 2336 1936
NheI G'CTAG_C
Cuts at: 0 4069 4272
Size: 4069 203
NspI r_CATG'y
Cuts at: 0 3898 4144 4272
Size: 3898 246 128
Fragments arranged by size:
3898 246 128
PasI CC'CwG_GG
Cuts at: 0 1972 3310 3466 4272
Size: 1972 1338 156 806
Fragments arranged by size:
1972 1338 806 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 360 3181 4272
Size: 360 2821 1091
Fragments arranged by size:
2821 1091 360
PfoI T'CCnGG_A
Cuts at: 0 2779 4272
Size: 2779 1493
PpuMI rG'GwC_Cy
Cuts at: 0 641 743 869 1351 1954 2902 3138 3471
Size: 641 102 126 482 603 948 236 333
Cuts at: 3471 4242 4272
Size: 771 30
Fragments arranged by size:
948 771 641 603 482 333 236 126
102 30
PshAI GACnn'nnGTC
Cuts at: 0 3005 4272
Size: 3005 1267
PspOMI G'GGCC_C
Cuts at: 0 3124 3160 3304 4114 4272
Size: 3124 36 144 810 158
Fragments arranged by size:
3124 810 158 144 36
PstI C_TGCA'G
Cuts at: 0 555 797 3007 4185 4272
Size: 555 242 2210 1178 87
Fragments arranged by size:
2210 1178 555 242 87
PvuII CAG'CTG
Cuts at: 0 2047 2708 3805 3925 4189 4272
Size: 2047 661 1097 120 264 83
Fragments arranged by size:
2047 1097 661 264 120 83
SacI G_AGCT'C
Cuts at: 0 2016 4272
Size: 2016 2256
SanDI GG'GwC_CC
Cuts at: 0 641 869 3471 4272
Size: 641 228 2602 801
Fragments arranged by size:
2602 801 641 228
SapI GCTCTTCn'nnn_
Cuts at: 0 86 2151 3269 4272
Size: 86 2065 1118 1003
Fragments arranged by size:
2065 1118 1003 86
SfcI C'TryA_G
Cuts at: 0 431 551 793 929 1680 2412 2445 2454
Size: 431 120 242 136 751 732 33 9
Cuts at: 2454 2568 2727 3003 3578 3860 4181 4272
Size: 114 159 276 575 282 321 91
Fragments arranged by size:
751 732 575 431 321 282 276 242
159 136 120 114 91 33 9
SfoI GGC'GCC
Cuts at: 0 58 2341 4272
Size: 58 2283 1931
Fragments arranged by size:
2283 1931 58
SmaI CCC'GGG
Cuts at: 0 2635 4272
Size: 2635 1637
SmlI C'TyrA_G
Cuts at: 0 2015 2325 2376 2625 3133 3592 4272
Size: 2015 310 51 249 508 459 680
Fragments arranged by size:
2015 680 508 459 310 249 51
SphI G_CATG'C
Cuts at: 0 3898 4272
Size: 3898 374
SspI AAT'ATT
Cuts at: 0 2548 4272
Size: 2548 1724
StuI AGG'CCT
Cuts at: 0 1219 1580 3790 4272
Size: 1219 361 2210 482
Fragments arranged by size:
2210 1219 482 361
StyI C'CwwG_G
Cuts at: 0 149 606 786 1119 1442 1575 1716 2106
Size: 149 457 180 333 323 133 141 390
Cuts at: 2106 4263 4272
Size: 2157 9
Fragments arranged by size:
2157 457 390 333 323 180 149 141
133 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1061 1192 4226 4272
Size: 1061 131 3034 46
Fragments arranged by size:
3034 1061 131 46
TatI w'GTAC_w
Cuts at: 0 107 555 2270 3722 4272
Size: 107 448 1715 1452 550
Fragments arranged by size:
1715 1452 550 448 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1798 1830 3069 3101 4272
Size: 1798 32 1239 32 1171
Fragments arranged by size:
1798 1239 1171 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 2107 2206 3042 4272
Size: 1020 1087 99 836 1230
Fragments arranged by size:
1230 1087 1020 836 99
XmaI C'CCGG_G
Cuts at: 0 2633 4272
Size: 2633 1639
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlwNI ApaI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SanDI SapI SfcI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI AscI AsiSI AvrII BaeI BcgI BciVI BfrBI
BmgBI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI
DrdI EagI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MluI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmnI
ZraI