Restriction map for
pF1KSDA0535
(Linear) (Six-base) MAPSORT of: KIAA0535.seq Check: 3823 from: 1 to: 3141
KIAA0535.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 146 734 2738 2833 3141
Size: 35 111 588 2004 95 308
Fragments arranged by size:
2004 588 308 111 95 35
AflII C'TTAA_G
Cuts at: 0 1845 2296 3141
Size: 1845 451 845
Fragments arranged by size:
1845 845 451
AflIII A'CryG_T
Cuts at: 0 1131 3141
Size: 1131 2010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 513 944 3141
Size: 513 431 2197
Fragments arranged by size:
2197 513 431
AlwNI CAG_nnn'CTG
Cuts at: 0 13 1723 1726 1794 2512 3141
Size: 13 1710 3 68 718 629
Fragments arranged by size:
1710 718 629 68 13 3
ApoI r'AATT_y
Cuts at: 0 403 441 724 903 1202 1423 1618 2076
Size: 403 38 283 179 299 221 195 458
Cuts at: 2076 3021 3141
Size: 945 120
Fragments arranged by size:
945 458 403 299 283 221 195 179
120 38
AvaI C'yCGr_G
Cuts at: 0 2325 3141
Size: 2325 816
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 345 378 3141
Size: 345 33 2763
Fragments arranged by size:
2763 345 33
BanI G'GyrC_C
Cuts at: 0 54 1172 1645 3141
Size: 54 1118 473 1496
Fragments arranged by size:
1496 1118 473 54
BanII G_rGCy'C
Cuts at: 0 83 374 1145 1663 2068 3141
Size: 83 291 771 518 405 1073
Fragments arranged by size:
1073 771 518 405 291 83
BbeI G_GCGC'C
Cuts at: 0 1649 3141
Size: 1649 1492
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 301 353 1053 1709 2038 2435 3141
Size: 221 80 52 700 656 329 397 706
Fragments arranged by size:
706 700 656 397 329 221 80 52
BfrBI ATG'CAT
Cuts at: 0 1215 2218 3141
Size: 1215 1003 923
Fragments arranged by size:
1215 1003 923
BglII A'GATC_T
Cuts at: 0 2145 3141
Size: 2145 996
BlpI GC'TnA_GC
Cuts at: 0 941 3141
Size: 941 2200
Bme1580I G_kGCm'C
Cuts at: 0 1105 1177 2333 3141
Size: 1105 72 1156 808
Fragments arranged by size:
1156 1105 808 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 1382 2053 2759 3141
Size: 1382 671 706 382
Fragments arranged by size:
1382 706 671 382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2584 3141
Size: 2584 557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 937 1015 2120 3141
Size: 318 619 78 1105 1021
Fragments arranged by size:
1105 1021 619 318 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 3141
Size: 1213 1928
BsaI GGTCTCn'nnnn_
Cuts at: 0 1087 3141
Size: 1087 2054
BsaAI yAC'GTr
Cuts at: 0 1134 2202 2334 3141
Size: 1134 1068 132 807
Fragments arranged by size:
1134 1068 807 132
BsaBI GATnn'nnATC
Cuts at: 0 1349 3141
Size: 1349 1792
BsaHI Gr'CG_yC
Cuts at: 0 1646 3141
Size: 1646 1495
BsaWI w'CCGG_w
Cuts at: 0 2368 3141
Size: 2368 773
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 1084 1114 1759 1789 2284 2314
Size: 601 30 453 30 645 30 495 30
Cuts at: 2314 3141
Size: 827
Fragments arranged by size:
827 645 601 495 453 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 552 850 2053 2056 2908 2911 3141
Size: 552 298 1203 3 852 3 230
Fragments arranged by size:
1203 852 552 298 230 3 3
BseYI C'CCAG_C
Cuts at: 0 605 812 1994 2628 3141
Size: 605 207 1182 634 513
Fragments arranged by size:
1182 634 605 513 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 614 1661 1865 3141
Size: 305 309 1047 204 1276
Fragments arranged by size:
1276 1047 309 305 204
BsiHKAI G_wGCw'C
Cuts at: 0 83 374 1001 1229 2068 3141
Size: 83 291 627 228 839 1073
Fragments arranged by size:
1073 839 627 291 228 83
BsmI GAATG_Cn'
Cuts at: 0 786 1946 1963 3094 3141
Size: 786 1160 17 1131 47
Fragments arranged by size:
1160 1131 786 47 17
Bsp1286I G_dGCh'C
Cuts at: 0 83 374 1001 1105 1145 1177 1229 1663
Size: 83 291 627 104 40 32 52 434
Cuts at: 1663 2068 2333 3141
Size: 405 265 808
Fragments arranged by size:
808 627 434 405 291 265 104 83
52 40 32
BspEI T'CCGG_A
Cuts at: 0 2368 3141
Size: 2368 773
BspHI T'CATG_A
Cuts at: 0 284 2726 3141
Size: 284 2442 415
Fragments arranged by size:
2442 415 284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2938 3141
Size: 2938 203
BsrDI GCAATG_nn'
Cuts at: 0 813 1340 2628 3141
Size: 813 527 1288 513
Fragments arranged by size:
1288 813 527 513
BsrGI T'GTAC_A
Cuts at: 0 3056 3141
Size: 3056 85
BssSI C'ACGA_G
Cuts at: 0 2378 3141
Size: 2378 763
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1723 1726 3141
Size: 1723 3 1415
Fragments arranged by size:
1723 1415 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1663 3141
Size: 1663 1478
BstYI r'GATC_y
Cuts at: 0 2145 3141
Size: 2145 996
BtgI C'CryG_G
Cuts at: 0 104 3141
Size: 104 3037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 497 2209 2333 3141
Size: 497 1712 124 808
Fragments arranged by size:
1712 808 497 124
BtsI GCAGTG_nn'
Cuts at: 0 228 1384 2235 3128 3141
Size: 228 1156 851 893 13
Fragments arranged by size:
1156 893 851 228 13
DraI TTT'AAA
Cuts at: 0 446 470 3141
Size: 446 24 2671
Fragments arranged by size:
2671 446 24
DraIII CAC_nnn'GTG
Cuts at: 0 1185 3141
Size: 1185 1956
EaeI y'GGCC_r
Cuts at: 0 1665 2196 2466 3141
Size: 1665 531 270 675
Fragments arranged by size:
1665 675 531 270
EarI CTCTTCn'nnn_
Cuts at: 0 109 519 866 1929 3141
Size: 109 410 347 1063 1212
Fragments arranged by size:
1212 1063 410 347 109
EcoICRI GAG'CTC
Cuts at: 0 81 372 2066 3141
Size: 81 291 1694 1075
Fragments arranged by size:
1694 1075 291 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 146 318 734 937 1015 2120 2738
Size: 35 111 172 416 203 78 1105 618
Cuts at: 2738 2833 3141
Size: 95 308
Fragments arranged by size:
1105 618 416 308 203 172 111 95
78 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 1640 2453 3141
Size: 889 751 813 688
Fragments arranged by size:
889 813 751 688
EcoO109I rG'GnC_Cy
Cuts at: 0 3004 3141
Size: 3004 137
EcoRI G'AATT_C
Cuts at: 0 1423 3141
Size: 1423 1718
HaeII r_GCGC'y
Cuts at: 0 1649 3141
Size: 1649 1492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 601 633 1759 1791 2666 2698
Size: 340 32 229 32 1126 32 875 32
Cuts at: 2698 3141
Size: 443
Fragments arranged by size:
1126 875 443 340 229 32 32 32
32
HincII GTy'rAC
Cuts at: 0 153 3141
Size: 153 2988
HindIII A'AGCT_T
Cuts at: 0 2131 2361 2664 3141
Size: 2131 230 303 477
Fragments arranged by size:
2131 477 303 230
KasI G'GCGC_C
Cuts at: 0 1645 3141
Size: 1645 1496
MfeI C'AATT_G
Cuts at: 0 926 1310 3141
Size: 926 384 1831
Fragments arranged by size:
1831 926 384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 1802 1936 2197 2329 2840 3141
Size: 644 1158 134 261 132 511 301
Fragments arranged by size:
1158 644 511 301 261 134 132
MscI TGG'CCA
Cuts at: 0 2198 2468 3141
Size: 2198 270 673
Fragments arranged by size:
2198 673 270
MslI CAynn'nnrTG
Cuts at: 0 515 1220 2181 2228 2786 2866 3052 3141
Size: 515 705 961 47 558 80 186 89
Fragments arranged by size:
961 705 558 515 186 89 80 47
MspA1I CmG'CkG
Cuts at: 0 124 1321 1723 1751 3141
Size: 124 1197 402 28 1390
Fragments arranged by size:
1390 1197 402 124 28
NarI GG'CG_CC
Cuts at: 0 1646 3141
Size: 1646 1495
NcoI C'CATG_G
Cuts at: 0 104 3141
Size: 104 3037
NdeI CA'TA_TG
Cuts at: 0 3033 3141
Size: 3033 108
NsiI A_TGCA'T
Cuts at: 0 1217 2220 3141
Size: 1217 1003 921
Fragments arranged by size:
1217 1003 921
NspI r_CATG'y
Cuts at: 0 1801 3141
Size: 1801 1340
PfoI T'CCnGG_A
Cuts at: 0 74 1868 2097 2572 3141
Size: 74 1794 229 475 569
Fragments arranged by size:
1794 569 475 229 74
PmlI CAC'GTG
Cuts at: 0 1134 2202 2334 3141
Size: 1134 1068 132 807
Fragments arranged by size:
1134 1068 807 132
PpuMI rG'GwC_Cy
Cuts at: 0 3004 3141
Size: 3004 137
PstI C_TGCA'G
Cuts at: 0 938 1320 1756 3141
Size: 938 382 436 1385
Fragments arranged by size:
1385 938 436 382
PvuII CAG'CTG
Cuts at: 0 124 1321 1723 3141
Size: 124 1197 402 1418
Fragments arranged by size:
1418 1197 402 124
SacI G_AGCT'C
Cuts at: 0 83 374 2068 3141
Size: 83 291 1694 1073
Fragments arranged by size:
1694 1073 291 83
SfcI C'TryA_G
Cuts at: 0 189 934 1316 1752 2013 3141
Size: 189 745 382 436 261 1128
Fragments arranged by size:
1128 745 436 382 261 189
SfoI GGC'GCC
Cuts at: 0 1647 3141
Size: 1647 1494
SmlI C'TyrA_G
Cuts at: 0 1228 1845 2296 3141
Size: 1228 617 451 845
Fragments arranged by size:
1228 845 617 451
SphI G_CATG'C
Cuts at: 0 1801 3141
Size: 1801 1340
StuI AGG'CCT
Cuts at: 0 1080 2107 3141
Size: 1080 1027 1034
Fragments arranged by size:
1080 1034 1027
StyI C'CwwG_G
Cuts at: 0 104 248 896 1075 1802 2414 2469 3141
Size: 104 144 648 179 727 612 55 672
Fragments arranged by size:
727 672 648 612 179 144 104 55
SwaI ATTT'AAAT
Cuts at: 0 446 3141
Size: 446 2695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1030 1300 1669 1723 2671 3141
Size: 1030 270 369 54 948 470
Fragments arranged by size:
1030 948 470 369 270 54
TatI w'GTAC_w
Cuts at: 0 3056 3141
Size: 3056 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 635 667 1084 1116 1893 1925
Size: 38 32 565 32 417 32 777 32
Cuts at: 1925 2284 2316 2379 2411 3141
Size: 359 32 63 32 730
Fragments arranged by size:
777 730 565 417 359 63 38 32
32 32 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2070 3141
Size: 2070 1071
XmnI GAAnn'nnTTC
Cuts at: 0 308 3141
Size: 308 2833
Enzymes that do cut:
AcuI AflII AflIII AlfI AlwNI ApoI AvaI BaeI
BanI BanII BbeI BbsI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
HindIII KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NsiI NspI PfoI PmlI PpuMI PstI
PvuII SacI SfcI SfoI SmlI SphI StuI StyI
SwaI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbvCI BcgI BciVI BclI BglI BmgBI BmtI
Bpu10I BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HpaI KpnI MluI
NaeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI Tth111I XcmI XhoI XmaI ZraI