Restriction map for
pF1KSDA0532
(Linear) (Six-base) MAPSORT of: KIAA0532.seq Check: 532 from: 1 to: 4521
KIAA0532.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 1752 3068 4521
Size: 1752 1316 1453
Fragments arranged by size:
1752 1453 1316
Acc65I G'GTAC_C
Cuts at: 0 3574 4521
Size: 3574 947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 150 324 1151 1978 3462 3794 4018
Size: 18 132 174 827 827 1484 332 224
Cuts at: 4018 4521
Size: 503
Fragments arranged by size:
1484 827 827 503 332 224 174 132
18
AflIII A'CryG_T
Cuts at: 0 47 4521
Size: 47 4474
AhdI GACnn_n'nnGTC
Cuts at: 0 4054 4346 4521
Size: 4054 292 175
Fragments arranged by size:
4054 292 175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3754 4521
Size: 3754 767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 79 89 111 4521
Size: 57 22 10 22 4410
Fragments arranged by size:
4410 57 22 22 10
AlwNI CAG_nnn'CTG
Cuts at: 0 2035 3717 4041 4521
Size: 2035 1682 324 480
Fragments arranged by size:
2035 1682 480 324
ApaI G_GGCC'C
Cuts at: 0 1422 3384 3511 4521
Size: 1422 1962 127 1010
Fragments arranged by size:
1962 1422 1010 127
ApaLI G'TGCA_C
Cuts at: 0 927 1567 3349 4202 4521
Size: 927 640 1782 853 319
Fragments arranged by size:
1782 927 853 640 319
ApoI r'AATT_y
Cuts at: 0 1258 2115 2303 3234 4477 4521
Size: 1258 857 188 931 1243 44
Fragments arranged by size:
1258 1243 931 857 188 44
AseI AT'TA_AT
Cuts at: 0 3011 4521
Size: 3011 1510
AvaI C'yCGr_G
Cuts at: 0 2110 2213 2727 3503 3685 3840 4521
Size: 2110 103 514 776 182 155 681
Fragments arranged by size:
2110 776 681 514 182 155 103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 34 67 4521
Size: 34 33 4454
Fragments arranged by size:
4454 34 33
BanI G'GyrC_C
Cuts at: 0 891 3574 4521
Size: 891 2683 947
Fragments arranged by size:
2683 947 891
BanII G_rGCy'C
Cuts at: 0 1422 3384 3511 4093 4521
Size: 1422 1962 127 582 428
Fragments arranged by size:
1962 1422 582 428 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 648 836 1778 2207 2526 3062 4458
Size: 330 318 188 942 429 319 536 1396
Cuts at: 4458 4521
Size: 63
Fragments arranged by size:
1396 942 536 429 330 319 318 188
63
BciVI GTATCCnnnnn_n'
Cuts at: 0 390 4521
Size: 390 4131
BclI T'GATC_A
Cuts at: 0 3765 4521
Size: 3765 756
BfrBI ATG'CAT
Cuts at: 0 1984 4521
Size: 1984 2537
BglI GCCn_nnn'nGGC
Cuts at: 0 3379 4521
Size: 3379 1142
BglII A'GATC_T
Cuts at: 0 2792 4521
Size: 2792 1729
BlpI GC'TnA_GC
Cuts at: 0 2681 3896 4521
Size: 2681 1215 625
Fragments arranged by size:
2681 1215 625
Bme1580I G_kGCm'C
Cuts at: 0 931 1422 1571 3353 3384 3511 4206 4521
Size: 931 491 149 1782 31 127 695 315
Fragments arranged by size:
1782 931 695 491 315 149 127 31
BmgBI CAC'GTC
Cuts at: 0 4074 4521
Size: 4074 447
BmrI ACTGGGnnnn_n'
Cuts at: 0 1557 4521
Size: 1557 2964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 2073 3519 3533 4521
Size: 1250 823 1446 14 988
Fragments arranged by size:
1446 1250 988 823 14
Bpu10I CC'TnA_GC
Cuts at: 0 4092 4460 4521
Size: 4092 368 61
Fragments arranged by size:
4092 368 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 941 3247 4228 4521
Size: 89 852 2306 981 293
Fragments arranged by size:
2306 981 852 293 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 3416 4521
Size: 3416 1105
BsaAI yAC'GTr
Cuts at: 0 3051 4521
Size: 3051 1470
BsaBI GATnn'nnATC
Cuts at: 0 631 4521
Size: 631 3890
BsaHI Gr'CG_yC
Cuts at: 0 3530 4521
Size: 3530 991
BsaWI w'CCGG_w
Cuts at: 0 2358 4521
Size: 2358 2163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1223 1253 3506 3536 4521
Size: 79 30 1114 30 2253 30 985
Fragments arranged by size:
2253 1114 985 79 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 1017 2905 3281 3658 3679 3902 4521
Size: 343 674 1888 376 377 21 223 619
Fragments arranged by size:
1888 674 619 377 376 343 223 21
BseYI C'CCAG_C
Cuts at: 0 1943 2010 3376 3398 3726 4360 4521
Size: 1943 67 1366 22 328 634 161
Fragments arranged by size:
1943 1366 634 328 161 67 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 207 1196 4258 4521
Size: 150 57 989 3062 263
Fragments arranged by size:
3062 989 263 150 57
BsiHKAI G_wGCw'C
Cuts at: 0 931 1571 2320 3353 3650 4139 4206 4521
Size: 931 640 749 1033 297 489 67 315
Fragments arranged by size:
1033 931 749 640 489 315 297 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3537 4521
Size: 3537 984
Bsp1286I G_dGCh'C
Cuts at: 0 931 1422 1571 2320 3353 3384 3511 3650
Size: 931 491 149 749 1033 31 127 139
Cuts at: 3650 4093 4139 4206 4521
Size: 443 46 67 315
Fragments arranged by size:
1033 931 749 491 443 315 149 139
127 67 46 31
BspEI T'CCGG_A
Cuts at: 0 2358 4521
Size: 2358 2163
BspHI T'CATG_A
Cuts at: 0 998 2328 4521
Size: 998 1330 2193
Fragments arranged by size:
2193 1330 998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2816 3045 3101 3156 4521
Size: 2816 229 56 55 1365
Fragments arranged by size:
2816 1365 229 56 55
BsrDI GCAATG_nn'
Cuts at: 0 3368 4521
Size: 3368 1153
BsrFI r'CCGG_y
Cuts at: 0 3626 3656 4521
Size: 3626 30 865
Fragments arranged by size:
3626 865 30
BsrGI T'GTAC_A
Cuts at: 0 1125 2514 3269 4521
Size: 1125 1389 755 1252
Fragments arranged by size:
1389 1252 1125 755
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3348 4521
Size: 3348 1173
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3189 3609 4521
Size: 3189 420 912
Fragments arranged by size:
3189 912 420
BstYI r'GATC_y
Cuts at: 0 89 1247 2792 4521
Size: 89 1158 1545 1729
Fragments arranged by size:
1729 1545 1158 89
BstZ17I GTA'TAC
Cuts at: 0 1753 3069 4521
Size: 1753 1316 1452
Fragments arranged by size:
1753 1452 1316
Bsu36I CC'TnA_GG
Cuts at: 0 320 4504 4521
Size: 320 4184 17
Fragments arranged by size:
4184 320 17
BtgI C'CryG_G
Cuts at: 0 1736 4484 4521
Size: 1736 2748 37
Fragments arranged by size:
2748 1736 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 212 4521
Size: 212 4309
BtsI GCAGTG_nn'
Cuts at: 0 284 376 1099 2651 3332 4521
Size: 284 92 723 1552 681 1189
Fragments arranged by size:
1552 1189 723 681 284 92
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3534 3569 4521
Size: 3534 35 952
Fragments arranged by size:
3534 952 35
DraI TTT'AAA
Cuts at: 0 2616 4521
Size: 2616 1905
EaeI y'GGCC_r
Cuts at: 0 387 4521
Size: 387 4134
EarI CTCTTCn'nnn_
Cuts at: 0 2747 4144 4521
Size: 2747 1397 377
Fragments arranged by size:
2747 1397 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 150 324 1151 1250 1978 2073 3462
Size: 18 132 174 827 99 728 95 1389
Cuts at: 3462 3519 3533 3794 4018 4521
Size: 57 14 261 224 503
Fragments arranged by size:
1389 827 728 503 261 224 174 132
99 95 57 18 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2027 3697 4521
Size: 2027 1670 824
Fragments arranged by size:
2027 1670 824
EcoO109I rG'GnC_Cy
Cuts at: 0 1419 1446 2566 2805 3185 3319 3380 3381
Size: 1419 27 1120 239 380 134 61 1
Cuts at: 3381 3507 3508 3714 4521
Size: 126 1 206 807
Fragments arranged by size:
1419 1120 807 380 239 206 134 126
61 27 1 1
EcoRI G'AATT_C
Cuts at: 0 1258 4521
Size: 1258 3263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2398 4521
Size: 2398 2123
HaeII r_GCGC'y
Cuts at: 0 453 2596 3966 4521
Size: 453 2143 1370 555
Fragments arranged by size:
2143 1370 555 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 635 667 993 1025 2207 2239 3308 3340
Size: 635 32 326 32 1182 32 1069 32
Cuts at: 3340 3616 3648 3889 3921 4040 4072 4282 4314
Size: 276 32 241 32 119 32 210 32
Cuts at: 4314 4332 4364 4521
Size: 18 32 157
Fragments arranged by size:
1182 1069 635 326 276 241 210 157
119 32 32 32 32 32 32 32
32 32 18
HindIII A'AGCT_T
Cuts at: 0 296 1526 4521
Size: 296 1230 2995
Fragments arranged by size:
2995 1230 296
KpnI G_GTAC'C
Cuts at: 0 3578 4521
Size: 3578 943
MfeI C'AATT_G
Cuts at: 0 3749 4521
Size: 3749 772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2915 2948 4521
Size: 2915 33 1573
Fragments arranged by size:
2915 1573 33
MscI TGG'CCA
Cuts at: 0 389 4521
Size: 389 4132
MslI CAynn'nnrTG
Cuts at: 0 889 2522 2542 3865 4521
Size: 889 1633 20 1323 656
Fragments arranged by size:
1633 1323 889 656 20
MspA1I CmG'CkG
Cuts at: 0 446 471 2680 3376 4364 4521
Size: 446 25 2209 696 988 157
Fragments arranged by size:
2209 988 696 446 157 25
NcoI C'CATG_G
Cuts at: 0 1736 4484 4521
Size: 1736 2748 37
Fragments arranged by size:
2748 1736 37
NdeI CA'TA_TG
Cuts at: 0 1291 1383 4521
Size: 1291 92 3138
Fragments arranged by size:
3138 1291 92
NsiI A_TGCA'T
Cuts at: 0 1986 4521
Size: 1986 2535
NspI r_CATG'y
Cuts at: 0 51 268 3988 4521
Size: 51 217 3720 533
Fragments arranged by size:
3720 533 217 51
PasI CC'CwG_GG
Cuts at: 0 1415 2746 4521
Size: 1415 1331 1775
Fragments arranged by size:
1775 1415 1331
PciI A'CATG_T
Cuts at: 0 47 4521
Size: 47 4474
PflMI CCAn_nnn'nTGG
Cuts at: 0 4440 4521
Size: 4440 81
PfoI T'CCnGG_A
Cuts at: 0 1077 1698 2120 4521
Size: 1077 621 422 2401
Fragments arranged by size:
2401 1077 621 422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 567 599 4521
Size: 79 32 456 32 3922
Fragments arranged by size:
3922 456 79 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1446 2566 2805 3319 4521
Size: 1446 1120 239 514 1202
Fragments arranged by size:
1446 1202 1120 514 239
PshAI GACnn'nnGTC
Cuts at: 0 1964 2207 4521
Size: 1964 243 2314
Fragments arranged by size:
2314 1964 243
PsiI TTA'TAA
Cuts at: 0 1817 2254 2831 4521
Size: 1817 437 577 1690
Fragments arranged by size:
1817 1690 577 437
PspOMI G'GGCC_C
Cuts at: 0 1418 3380 3507 4521
Size: 1418 1962 127 1014
Fragments arranged by size:
1962 1418 1014 127
PspXI vC'TCGA_Gb
Cuts at: 0 2213 4521
Size: 2213 2308
PstI C_TGCA'G
Cuts at: 0 2813 4404 4521
Size: 2813 1591 117
Fragments arranged by size:
2813 1591 117
PvuII CAG'CTG
Cuts at: 0 446 471 2680 4364 4521
Size: 446 25 2209 1684 157
Fragments arranged by size:
2209 1684 446 157 25
SapI GCTCTTCn'nnn_
Cuts at: 0 2747 4144 4521
Size: 2747 1397 377
Fragments arranged by size:
2747 1397 377
ScaI AGT'ACT
Cuts at: 0 1976 4521
Size: 1976 2545
SexAI A'CCwGG_T
Cuts at: 0 4439 4521
Size: 4439 82
SfcI C'TryA_G
Cuts at: 0 654 722 1480 2066 2147 2809 4400 4521
Size: 654 68 758 586 81 662 1591 121
Fragments arranged by size:
1591 758 662 654 586 121 81 68
SmaI CCC'GGG
Cuts at: 0 2729 3505 4521
Size: 2729 776 1016
Fragments arranged by size:
2729 1016 776
SmlI C'TyrA_G
Cuts at: 0 104 956 2213 3262 4243 4521
Size: 104 852 1257 1049 981 278
Fragments arranged by size:
1257 1049 981 852 278 104
SspI AAT'ATT
Cuts at: 0 411 1811 4521
Size: 411 1400 2710
Fragments arranged by size:
2710 1400 411
StuI AGG'CCT
Cuts at: 0 4177 4521
Size: 4177 344
StyI C'CwwG_G
Cuts at: 0 255 1736 3188 3827 4484 4521
Size: 255 1481 1452 639 657 37
Fragments arranged by size:
1481 1452 657 639 255 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2677 4521
Size: 2677 1844
TatI w'GTAC_w
Cuts at: 0 440 563 615 872 1125 1974 2514 3269
Size: 440 123 52 257 253 849 540 755
Cuts at: 3269 4310 4521
Size: 1041 211
Fragments arranged by size:
1041 849 755 540 440 257 253 211
123 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3084 3116 3506 3538 4521
Size: 3084 32 390 32 983
Fragments arranged by size:
3084 983 390 32 32
XbaI T'CTAG_A
Cuts at: 0 123 2412 4521
Size: 123 2289 2109
Fragments arranged by size:
2289 2109 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 2094 2931 4521
Size: 201 1893 837 1590
Fragments arranged by size:
1893 1590 837 201
XhoI C'TCGA_G
Cuts at: 0 2213 4521
Size: 2213 2308
XmaI C'CCGG_G
Cuts at: 0 2727 3503 4521
Size: 2727 776 1018
Fragments arranged by size:
2727 1018 776
XmnI GAAnn'nnTTC
Cuts at: 0 163 410 579 1529 2888 4521
Size: 163 247 169 950 1359 1633
Fragments arranged by size:
1633 1359 950 247 169 163
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HindIII KpnI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI
PfoI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuII SapI ScaI SexAI SfcI SmaI SmlI SspI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AscI
AsiSI AvrII BamHI BbeI BbvCI BcgI BmtI BplI
BsiEI BsiWI BsmI BsrBI BssHII BssSI BstBI BstEII
ClaI DraIII DrdI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HincII HpaI KasI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PmeI PmlI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
Tth111I ZraI