Restriction map for
pF1KSDA0513
(Linear) (Six-base) MAPSORT of: KIAA0513.seq Check: 6060 from: 1 to: 1233
KIAA0513.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 256 1233
Size: 256 977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 775 904 1171 1233
Size: 712 63 129 267 62
Fragments arranged by size:
712 267 129 63 62
AfeI AGC'GCT
Cuts at: 0 430 1233
Size: 430 803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 500 1233
Size: 500 733
AlwNI CAG_nnn'CTG
Cuts at: 0 588 1233
Size: 588 645
ApaI G_GGCC'C
Cuts at: 0 764 1233
Size: 764 469
AvaI C'yCGr_G
Cuts at: 0 602 838 1233
Size: 602 236 395
Fragments arranged by size:
602 395 236
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 405 438 1233
Size: 405 33 795
Fragments arranged by size:
795 405 33
BanI G'GyrC_C
Cuts at: 0 51 75 1019 1233
Size: 51 24 944 214
Fragments arranged by size:
944 214 51 24
BanII G_rGCy'C
Cuts at: 0 28 764 1233
Size: 28 736 469
Fragments arranged by size:
736 469 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 791 1233
Size: 791 442
BglI GCCn_nnn'nGGC
Cuts at: 0 900 1233
Size: 900 333
BglII A'GATC_T
Cuts at: 0 314 863 1233
Size: 314 549 370
Fragments arranged by size:
549 370 314
Bme1580I G_kGCm'C
Cuts at: 0 426 764 1093 1233
Size: 426 338 329 140
Fragments arranged by size:
426 338 329 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 249 1233
Size: 198 51 984
Fragments arranged by size:
984 198 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 344 763 1010 1233
Size: 91 253 419 247 223
Fragments arranged by size:
419 253 247 223 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 265 739 1233
Size: 130 135 474 494
Fragments arranged by size:
494 474 135 130
BsaAI yAC'GTr
Cuts at: 0 306 417 1233
Size: 306 111 816
Fragments arranged by size:
816 306 111
BsaWI w'CCGG_w
Cuts at: 0 1055 1233
Size: 1055 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 48 78 1003 1033 1233
Size: 48 30 925 30 200
Fragments arranged by size:
925 200 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 225 361 1009 1012 1015 1051 1233
Size: 207 18 136 648 3 3 36 182
Fragments arranged by size:
648 207 182 136 36 18 3 3
BseYI C'CCAG_C
Cuts at: 0 425 1086 1233
Size: 425 661 147
Fragments arranged by size:
661 425 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1233
Size: 496 737
BsiHKAI G_wGCw'C
Cuts at: 0 250 1233
Size: 250 983
BsmI GAATG_Cn'
Cuts at: 0 927 1233
Size: 927 306
Bsp1286I G_dGCh'C
Cuts at: 0 28 250 426 764 1093 1233
Size: 28 222 176 338 329 140
Fragments arranged by size:
338 329 222 176 140 28
BspHI T'CATG_A
Cuts at: 0 542 1233
Size: 542 691
BsrBI CCG'CTC
Cuts at: 0 684 1233
Size: 684 549
BsrDI GCAATG_nn'
Cuts at: 0 1146 1233
Size: 1146 87
BsrGI T'GTAC_A
Cuts at: 0 872 1233
Size: 872 361
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1107 1233
Size: 1107 126
BstYI r'GATC_y
Cuts at: 0 314 863 1233
Size: 314 549 370
Fragments arranged by size:
549 370 314
Bsu36I CC'TnA_GG
Cuts at: 0 47 1233
Size: 47 1186
BtgI C'CryG_G
Cuts at: 0 20 614 1233
Size: 20 594 619
Fragments arranged by size:
619 594 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 660 1233
Size: 116 544 573
Fragments arranged by size:
573 544 116
BtsI GCAGTG_nn'
Cuts at: 0 384 494 1233
Size: 384 110 739
Fragments arranged by size:
739 384 110
DrdI GACnn_nn'nnGTC
Cuts at: 0 144 1233
Size: 144 1089
EaeI y'GGCC_r
Cuts at: 0 659 704 767 1223 1233
Size: 659 45 63 456 10
Fragments arranged by size:
659 456 63 45 10
EarI CTCTTCn'nnn_
Cuts at: 0 66 263 1000 1178 1233
Size: 66 197 737 178 55
Fragments arranged by size:
737 197 178 66 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 344 712 763 775 904 1010 1171
Size: 91 253 368 51 12 129 106 161
Cuts at: 1171 1233
Size: 62
Fragments arranged by size:
368 253 161 129 106 91 62 51
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 705 1233
Size: 705 528
EcoO109I rG'GnC_Cy
Cuts at: 0 15 760 761 1233
Size: 15 745 1 472
Fragments arranged by size:
745 472 15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 747 1233
Size: 747 486
HaeII r_GCGC'y
Cuts at: 0 432 1233
Size: 432 801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 1233
Size: 109 32 1092
Fragments arranged by size:
1092 109 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 263 1233
Size: 245 18 970
Fragments arranged by size:
970 245 18
MscI TGG'CCA
Cuts at: 0 769 1225 1233
Size: 769 456 8
Fragments arranged by size:
769 456 8
MslI CAynn'nnrTG
Cuts at: 0 547 1233
Size: 547 686
MspA1I CmG'CkG
Cuts at: 0 585 616 653 1086 1233
Size: 585 31 37 433 147
Fragments arranged by size:
585 433 147 37 31
NspI r_CATG'y
Cuts at: 0 1110 1233
Size: 1110 123
PasI CC'CwG_GG
Cuts at: 0 111 815 1233
Size: 111 704 418
Fragments arranged by size:
704 418 111
PpuMI rG'GwC_Cy
Cuts at: 0 15 1233
Size: 15 1218
PspOMI G'GGCC_C
Cuts at: 0 760 1233
Size: 760 473
PstI C_TGCA'G
Cuts at: 0 95 376 1233
Size: 95 281 857
Fragments arranged by size:
857 281 95
PvuII CAG'CTG
Cuts at: 0 585 653 1086 1233
Size: 585 68 433 147
Fragments arranged by size:
585 433 147 68
SacII CC_GC'GG
Cuts at: 0 617 1233
Size: 617 616
SapI GCTCTTCn'nnn_
Cuts at: 0 1178 1233
Size: 1178 55
ScaI AGT'ACT
Cuts at: 0 370 1233
Size: 370 863
SfcI C'TryA_G
Cuts at: 0 91 372 1233
Size: 91 281 861
Fragments arranged by size:
861 281 91
SmaI CCC'GGG
Cuts at: 0 604 1233
Size: 604 629
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 69 1233
Size: 69 1164
TatI w'GTAC_w
Cuts at: 0 368 872 1233
Size: 368 504 361
Fragments arranged by size:
504 368 361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 48 80 1003 1035 1233
Size: 48 32 923 32 198
Fragments arranged by size:
923 198 48 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 940 1233
Size: 940 293
XmaI C'CCGG_G
Cuts at: 0 602 1233
Size: 602 631
XmnI GAAnn'nnTTC
Cuts at: 0 316 1233
Size: 316 917
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI AvaI BaeI
BanI BanII BbsI BglI BglII Bme1580I BplI BpmI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI BstAPI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MslI
MspA1I NspI PasI PpuMI PspOMI PstI PvuII SacII
SapI ScaI SfcI SmaI TaqII TatI TstI Tth111I
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaHI BsiEI
BsiWI BsmBI BspEI BspMI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI XbaI XcmI
XhoI ZraI