Restriction map for
pF1KA0456
(Linear) (Six-base) MAPSORT of: KIAA0456.seq Check: 3932 from: 1 to: 3213
KIAA0456.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AatII G_ACGT'C
Cuts at: 0 7 3213
Size: 7 3206
AccI GT'mk_AC
Cuts at: 0 2066 3190 3213
Size: 2066 1124 23
Fragments arranged by size:
2066 1124 23
AclI AA'CG_TT
Cuts at: 0 635 3213
Size: 635 2578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1714 2444 2749 3213
Size: 751 963 730 305 464
Fragments arranged by size:
963 751 730 464 305
AflIII A'CryG_T
Cuts at: 0 503 654 3213
Size: 503 151 2559
Fragments arranged by size:
2559 503 151
AhdI GACnn_n'nnGTC
Cuts at: 0 1971 3213
Size: 1971 1242
AlwNI CAG_nnn'CTG
Cuts at: 0 2527 3213
Size: 2527 686
ApaI G_GGCC'C
Cuts at: 0 1937 2062 3213
Size: 1937 125 1151
Fragments arranged by size:
1937 1151 125
ApoI r'AATT_y
Cuts at: 0 16 1571 3213
Size: 16 1555 1642
Fragments arranged by size:
1642 1555 16
AvaI C'yCGr_G
Cuts at: 0 2231 3010 3213
Size: 2231 779 203
Fragments arranged by size:
2231 779 203
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 44 77 3213
Size: 44 33 3136
Fragments arranged by size:
3136 44 33
BamHI G'GATC_C
Cuts at: 0 1588 3213
Size: 1588 1625
BanII G_rGCy'C
Cuts at: 0 479 670 873 1794 1937 2062 2189 2936
Size: 479 191 203 921 143 125 127 747
Cuts at: 2936 2975 3017 3213
Size: 39 42 196
Fragments arranged by size:
921 747 479 203 196 191 143 127
125 42 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 414 499 783 999 1171 2138 2767 3126
Size: 414 85 284 216 172 967 629 359
Cuts at: 3126 3213
Size: 87
Fragments arranged by size:
967 629 414 359 284 216 172 87
85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2182 2216 3213
Size: 2182 34 997
Fragments arranged by size:
2182 997 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1543 3213
Size: 1543 1670
BclI T'GATC_A
Cuts at: 0 828 2000 3213
Size: 828 1172 1213
Fragments arranged by size:
1213 1172 828
BfrBI ATG'CAT
Cuts at: 0 784 3213
Size: 784 2429
BlpI GC'TnA_GC
Cuts at: 0 77 2569 3213
Size: 77 2492 644
Fragments arranged by size:
2492 644 77
Bme1580I G_kGCm'C
Cuts at: 0 1074 1937 2062 2693 3213
Size: 1074 863 125 631 520
Fragments arranged by size:
1074 863 631 520 125
BmgBI CAC'GTC
Cuts at: 0 2913 2966 3213
Size: 2913 53 247
Fragments arranged by size:
2913 247 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 2456 2944 3213
Size: 2456 488 269
Fragments arranged by size:
2456 488 269
BmtI G_CTAG'C
Cuts at: 0 2615 3213
Size: 2615 598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 498 3213
Size: 498 2715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 220 266 1393 1482 2074 2961 3213
Size: 130 90 46 1127 89 592 887 252
Fragments arranged by size:
1127 887 592 252 130 90 89 46
Bpu10I CC'TnA_GC
Cuts at: 0 2828 3213
Size: 2828 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2986 3213
Size: 2986 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 1281 3213
Size: 1281 1932
BsaAI yAC'GTr
Cuts at: 0 1989 2217 3213
Size: 1989 228 996
Fragments arranged by size:
1989 996 228
BsaBI GATnn'nnATC
Cuts at: 0 2486 3213
Size: 2486 727
BsaHI Gr'CG_yC
Cuts at: 0 4 3213
Size: 4 3209
BsaWI w'CCGG_w
Cuts at: 0 659 1535 1802 2537 2756 3213
Size: 659 876 267 735 219 457
Fragments arranged by size:
876 735 659 457 267 219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1234 1264 2578 2608 3213
Size: 1234 30 1314 30 605
Fragments arranged by size:
1314 1234 605 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 577 661 678 1329 1646 2090 2580
Size: 292 285 84 17 651 317 444 490
Cuts at: 2580 2583 2989 3213
Size: 3 406 224
Fragments arranged by size:
651 490 444 406 317 292 285 224
84 17 3
BseYI C'CCAG_C
Cuts at: 0 1061 1073 1300 1648 2630 2993 3043 3213
Size: 1061 12 227 348 982 363 50 170
Fragments arranged by size:
1061 982 363 348 227 170 50 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1780 2971 3213
Size: 1780 1191 242
Fragments arranged by size:
1780 1191 242
BsiHKAI G_wGCw'C
Cuts at: 0 78 479 531 670 1794 2162 3213
Size: 78 401 52 139 1124 368 1051
Fragments arranged by size:
1124 1051 401 368 139 78 52
BsmI GAATG_Cn'
Cuts at: 0 948 3213
Size: 948 2265
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 3213
Size: 11 3202
Bsp1286I G_dGCh'C
Cuts at: 0 78 479 531 670 873 1074 1794 1937
Size: 78 401 52 139 203 201 720 143
Cuts at: 1937 2062 2162 2189 2693 2936 2975 3017 3213
Size: 125 100 27 504 243 39 42 196
Fragments arranged by size:
720 504 401 243 203 201 196 143
139 125 100 78 52 42 39 27
BspEI T'CCGG_A
Cuts at: 0 659 1802 2537 2756 3213
Size: 659 1143 735 219 457
Fragments arranged by size:
1143 735 659 457 219
BspHI T'CATG_A
Cuts at: 0 1289 1746 1844 2639 3213
Size: 1289 457 98 795 574
Fragments arranged by size:
1289 795 574 457 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1787 3213
Size: 1787 1426
BsrBI CCG'CTC
Cuts at: 0 2446 3213
Size: 2446 767
BsrDI GCAATG_nn'
Cuts at: 0 1304 2198 2822 3213
Size: 1304 894 624 391
Fragments arranged by size:
1304 894 624 391
BsrFI r'CCGG_y
Cuts at: 0 599 2303 3213
Size: 599 1704 910
Fragments arranged by size:
1704 910 599
BsrGI T'GTAC_A
Cuts at: 0 384 506 995 3213
Size: 384 122 489 2218
Fragments arranged by size:
2218 489 384 122
BssSI C'ACGA_G
Cuts at: 0 2166 3213
Size: 2166 1047
BstAPI GCAn_nnn'nTGC
Cuts at: 0 135 781 3213
Size: 135 646 2432
Fragments arranged by size:
2432 646 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2052 3213
Size: 2052 1161
BstYI r'GATC_y
Cuts at: 0 68 1588 2753 3213
Size: 68 1520 1165 460
Fragments arranged by size:
1520 1165 460 68
Bsu36I CC'TnA_GG
Cuts at: 0 2816 3213
Size: 2816 397
BtgI C'CryG_G
Cuts at: 0 626 1253 1271 2562 2807 3213
Size: 626 627 18 1291 245 406
Fragments arranged by size:
1291 627 626 406 245 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2186 3036 3213
Size: 2186 850 177
Fragments arranged by size:
2186 850 177
BtsI GCAGTG_nn'
Cuts at: 0 118 2898 3213
Size: 118 2780 315
Fragments arranged by size:
2780 315 118
DraI TTT'AAA
Cuts at: 0 417 3213
Size: 417 2796
DrdI GACnn_nn'nnGTC
Cuts at: 0 2818 3213
Size: 2818 395
EaeI y'GGCC_r
Cuts at: 0 224 435 629 3213
Size: 224 211 194 2584
Fragments arranged by size:
2584 224 211 194
EarI CTCTTCn'nnn_
Cuts at: 0 1148 1732 1886 3213
Size: 1148 584 154 1327
Fragments arranged by size:
1327 1148 584 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 565 3213
Size: 565 2648
EcoICRI GAG'CTC
Cuts at: 0 477 668 1792 3213
Size: 477 191 1124 1421
Fragments arranged by size:
1421 1124 477 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 220 266 751 1393 1482 1714 2074
Size: 130 90 46 485 642 89 232 360
Cuts at: 2074 2444 2749 2961 3213
Size: 370 305 212 252
Fragments arranged by size:
642 485 370 360 305 252 232 212
130 90 89 46
EcoO109I rG'GnC_Cy
Cuts at: 0 141 465 1638 1934 2058 2059 3006 3213
Size: 141 324 1173 296 124 1 947 207
Fragments arranged by size:
1173 947 324 296 207 141 124 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1719 1758 3213
Size: 1719 39 1455
Fragments arranged by size:
1719 1455 39
HaeII r_GCGC'y
Cuts at: 0 984 3035 3213
Size: 984 2051 178
Fragments arranged by size:
2051 984 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 1957 1989 2578 2610 3213
Size: 60 32 1865 32 589 32 603
Fragments arranged by size:
1865 603 589 60 32 32 32
HincII GTy'rAC
Cuts at: 0 288 3168 3213
Size: 288 2880 45
Fragments arranged by size:
2880 288 45
HindIII A'AGCT_T
Cuts at: 0 1696 2787 3213
Size: 1696 1091 426
Fragments arranged by size:
1696 1091 426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2309 3213
Size: 2309 904
MscI TGG'CCA
Cuts at: 0 226 3213
Size: 226 2987
MslI CAynn'nnrTG
Cuts at: 0 991 1357 1991 3213
Size: 991 366 634 1222
Fragments arranged by size:
1222 991 634 366
MspA1I CmG'CkG
Cuts at: 0 1446 2277 2456 2773 3213
Size: 1446 831 179 317 440
Fragments arranged by size:
1446 831 440 317 179
NaeI GCC'GGC
Cuts at: 0 2305 3213
Size: 2305 908
NcoI C'CATG_G
Cuts at: 0 1253 2562 2807 3213
Size: 1253 1309 245 406
Fragments arranged by size:
1309 1253 406 245
NgoMIV G'CCGG_C
Cuts at: 0 2303 3213
Size: 2303 910
NheI G'CTAG_C
Cuts at: 0 2611 3213
Size: 2611 602
NsiI A_TGCA'T
Cuts at: 0 786 3213
Size: 786 2427
NspI r_CATG'y
Cuts at: 0 507 658 3213
Size: 507 151 2555
Fragments arranged by size:
2555 507 151
PasI CC'CwG_GG
Cuts at: 0 1431 2045 2599 3213
Size: 1431 614 554 614
Fragments arranged by size:
1431 614 614 554
PciI A'CATG_T
Cuts at: 0 503 654 3213
Size: 503 151 2559
Fragments arranged by size:
2559 503 151
PflMI CCAn_nnn'nTGG
Cuts at: 0 1671 2527 3213
Size: 1671 856 686
Fragments arranged by size:
1671 856 686
PfoI T'CCnGG_A
Cuts at: 0 146 1193 3213
Size: 146 1047 2020
Fragments arranged by size:
2020 1047 146
PmlI CAC'GTG
Cuts at: 0 1989 3213
Size: 1989 1224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1709 1741 3213
Size: 1709 32 1472
Fragments arranged by size:
1709 1472 32
PpuMI rG'GwC_Cy
Cuts at: 0 141 465 1638 3006 3213
Size: 141 324 1173 1368 207
Fragments arranged by size:
1368 1173 324 207 141
PspOMI G'GGCC_C
Cuts at: 0 1933 2058 3213
Size: 1933 125 1155
Fragments arranged by size:
1933 1155 125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 463 495 3213
Size: 463 32 2718
Fragments arranged by size:
2718 463 32
PstI C_TGCA'G
Cuts at: 0 1121 1424 1784 3213
Size: 1121 303 360 1429
Fragments arranged by size:
1429 1121 360 303
PvuII CAG'CTG
Cuts at: 0 2456 3213
Size: 2456 757
SacI G_AGCT'C
Cuts at: 0 479 670 1794 3213
Size: 479 191 1124 1419
Fragments arranged by size:
1419 1124 479 191
SbfI CC_TGCA'GG
Cuts at: 0 1784 3213
Size: 1784 1429
ScaI AGT'ACT
Cuts at: 0 757 799 3039 3213
Size: 757 42 2240 174
Fragments arranged by size:
2240 757 174 42
SexAI A'CCwGG_T
Cuts at: 0 311 1967 3213
Size: 311 1656 1246
Fragments arranged by size:
1656 1246 311
SfcI C'TryA_G
Cuts at: 0 1117 1420 1558 1780 2067 3213
Size: 1117 303 138 222 287 1146
Fragments arranged by size:
1146 1117 303 287 222 138
SmlI C'TyrA_G
Cuts at: 0 3001 3213
Size: 3001 212
StyI C'CwwG_G
Cuts at: 0 242 1253 1733 2562 2807 2927 3213
Size: 242 1011 480 829 245 120 286
Fragments arranged by size:
1011 829 480 286 245 242 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3007 3213
Size: 3007 206
TatI w'GTAC_w
Cuts at: 0 384 506 713 755 797 995 1241 2339
Size: 384 122 207 42 42 198 246 1098
Cuts at: 2339 3037 3205 3213
Size: 698 168 8
Fragments arranged by size:
1098 698 384 246 207 198 168 122
42 42 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 1580 1612 3213
Size: 97 32 1451 32 1601
Fragments arranged by size:
1601 1451 97 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2368 3213
Size: 2368 845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 290 3213
Size: 290 2923
XmnI GAAnn'nnTTC
Cuts at: 0 2032 3213
Size: 2032 1181
ZraI GAC'GTC
Cuts at: 0 5 3213
Size: 5 3208
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AhdI AlwNI ApaI
ApoI AvaI BaeI BamHI BanII BbsI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuII SacI SbfI ScaI SexAI SfcI SmlI StyI
TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BanI BbeI BbvCI
BglI BglII BsiEI BsiWI BssHII BstBI BstEII BstZ17I
ClaI CspCI DraIII EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NarI
NdeI NotI NruI PacI PmeI PshAI PsiI PspXI
PvuI RsrII SacII SalI SanDI SapI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI