Restriction map for
pF1KA0455
(Linear) (Six-base) MAPSORT of: KIAA0455.seq Check: 6993 from: 1 to: 2289
KIAA0455.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1221 1808 1937 2289
Size: 1221 587 129 352
Fragments arranged by size:
1221 587 352 129
AhdI GACnn_n'nnGTC
Cuts at: 0 523 2289
Size: 523 1766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1264 2289
Size: 1264 1025
AlwNI CAG_nnn'CTG
Cuts at: 0 706 2289
Size: 706 1583
ApaLI G'TGCA_C
Cuts at: 0 97 289 1600 2289
Size: 97 192 1311 689
Fragments arranged by size:
1311 689 192 97
ApoI r'AATT_y
Cuts at: 0 425 672 984 2289
Size: 425 247 312 1305
Fragments arranged by size:
1305 425 312 247
AseI AT'TA_AT
Cuts at: 0 482 2289
Size: 482 1807
AvaI C'yCGr_G
Cuts at: 0 109 127 1658 2289
Size: 109 18 1531 631
Fragments arranged by size:
1531 631 109 18
BanI G'GyrC_C
Cuts at: 0 49 2289
Size: 49 2240
BanII G_rGCy'C
Cuts at: 0 476 1210 1850 1988 2289
Size: 476 734 640 138 301
Fragments arranged by size:
734 640 476 301 138
BbeI G_GCGC'C
Cuts at: 0 53 2289
Size: 53 2236
BbsI GAAGACnn'nnnn_
Cuts at: 0 270 840 1283 2289
Size: 270 570 443 1006
Fragments arranged by size:
1006 570 443 270
BbvCI CC'TCA_GC
Cuts at: 0 85 2289
Size: 85 2204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 162 2289
Size: 128 34 2127
Fragments arranged by size:
2127 128 34
BclI T'GATC_A
Cuts at: 0 176 2289
Size: 176 2113
BglI GCCn_nnn'nGGC
Cuts at: 0 1554 2289
Size: 1554 735
BlpI GC'TnA_GC
Cuts at: 0 376 2289
Size: 376 1913
Bme1580I G_kGCm'C
Cuts at: 0 101 293 1604 2289
Size: 101 192 1311 685
Fragments arranged by size:
1311 685 192 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 907 989 1341 1844 2113 2289
Size: 907 82 352 503 269 176
Fragments arranged by size:
907 503 352 269 176 82
BmtI G_CTAG'C
Cuts at: 0 1151 2289
Size: 1151 1138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 2289
Size: 1045 1244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 509 1571 2066 2289
Size: 88 421 1062 495 223
Fragments arranged by size:
1062 495 421 223 88
Bpu10I CC'TnA_GC
Cuts at: 0 85 699 1400 2289
Size: 85 614 701 889
Fragments arranged by size:
889 701 614 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 1040 1469 1806 2289
Size: 1040 429 337 483
Fragments arranged by size:
1040 483 429 337
BsaHI Gr'CG_yC
Cuts at: 0 50 1025 2289
Size: 50 975 1264
Fragments arranged by size:
1264 975 50
BsaWI w'CCGG_w
Cuts at: 0 314 2289
Size: 314 1975
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 959 1330 1627 2289
Size: 85 874 371 297 662
Fragments arranged by size:
874 662 371 297 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1791 2289
Size: 1791 498
BsiHKAI G_wGCw'C
Cuts at: 0 101 293 1604 1988 2289
Size: 101 192 1311 384 301
Fragments arranged by size:
1311 384 301 192 101
BsmI GAATG_Cn'
Cuts at: 0 1301 2289
Size: 1301 988
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1016 2140 2289
Size: 1016 1124 149
Fragments arranged by size:
1124 1016 149
Bsp1286I G_dGCh'C
Cuts at: 0 101 293 476 1210 1604 1850 1988 2289
Size: 101 192 183 734 394 246 138 301
Fragments arranged by size:
734 394 301 246 192 183 138 101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 405 2289
Size: 405 1884
BsrBI CCG'CTC
Cuts at: 0 1324 2289
Size: 1324 965
BsrDI GCAATG_nn'
Cuts at: 0 2082 2289
Size: 2082 207
BssHII G'CGCG_C
Cuts at: 0 6 2289
Size: 6 2283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 763 2289
Size: 763 1526
BstEII G'GTnAC_C
Cuts at: 0 1230 2289
Size: 1230 1059
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1489 2289
Size: 1489 800
BstYI r'GATC_y
Cuts at: 0 703 1056 1423 1904 2289
Size: 703 353 367 481 385
Fragments arranged by size:
703 481 385 367 353
Bsu36I CC'TnA_GG
Cuts at: 0 1030 1610 2289
Size: 1030 580 679
Fragments arranged by size:
1030 679 580
BtgI C'CryG_G
Cuts at: 0 27 1201 1211 1482 2091 2289
Size: 27 1174 10 271 609 198
Fragments arranged by size:
1174 609 271 198 27 10
BtsI GCAGTG_nn'
Cuts at: 0 1101 1935 2289
Size: 1101 834 354
Fragments arranged by size:
1101 834 354
DraIII CAC_nnn'GTG
Cuts at: 0 1349 2289
Size: 1349 940
EaeI y'GGCC_r
Cuts at: 0 24 2289
Size: 24 2265
EcoICRI GAG'CTC
Cuts at: 0 1986 2289
Size: 1986 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 509 1221 1571 1808 1937 2066 2289
Size: 88 421 712 350 237 129 129 223
Fragments arranged by size:
712 421 350 237 223 129 129 88
EcoO109I rG'GnC_Cy
Cuts at: 0 1586 2289
Size: 1586 703
EcoRI G'AATT_C
Cuts at: 0 425 2289
Size: 425 1864
EcoRV GAT'ATC
Cuts at: 0 582 604 2289
Size: 582 22 1685
Fragments arranged by size:
1685 582 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1398 2289
Size: 1398 891
HaeII r_GCGC'y
Cuts at: 0 53 60 1523 2289
Size: 53 7 1463 766
Fragments arranged by size:
1463 766 53 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 682 695 714 727 1135 1167
Size: 509 32 141 13 19 13 408 32
Cuts at: 1167 2137 2169 2289
Size: 970 32 120
Fragments arranged by size:
970 509 408 141 120 32 32 32
19 13 13
HindIII A'AGCT_T
Cuts at: 0 826 2289
Size: 826 1463
KasI G'GCGC_C
Cuts at: 0 49 2289
Size: 49 2240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 2289
Size: 444 1845
MslI CAynn'nnrTG
Cuts at: 0 1142 1432 1741 2289
Size: 1142 290 309 548
Fragments arranged by size:
1142 548 309 290
MspA1I CmG'CkG
Cuts at: 0 20 29 47 89 591 778 2289
Size: 20 9 18 42 502 187 1511
Fragments arranged by size:
1511 502 187 42 20 18 9
NarI GG'CG_CC
Cuts at: 0 50 2289
Size: 50 2239
NcoI C'CATG_G
Cuts at: 0 1211 1482 2289
Size: 1211 271 807
Fragments arranged by size:
1211 807 271
NheI G'CTAG_C
Cuts at: 0 1147 2289
Size: 1147 1142
NspI r_CATG'y
Cuts at: 0 330 757 1548 2289
Size: 330 427 791 741
Fragments arranged by size:
791 741 427 330
PfoI T'CCnGG_A
Cuts at: 0 1936 2289
Size: 1936 353
PpuMI rG'GwC_Cy
Cuts at: 0 1586 2289
Size: 1586 703
PshAI GACnn'nnGTC
Cuts at: 0 317 2289
Size: 317 1972
PsiI TTA'TAA
Cuts at: 0 2282 2289
Size: 2282 7
PvuII CAG'CTG
Cuts at: 0 89 591 778 2289
Size: 89 502 187 1511
Fragments arranged by size:
1511 502 187 89
SacI G_AGCT'C
Cuts at: 0 1988 2289
Size: 1988 301
SacII CC_GC'GG
Cuts at: 0 30 2289
Size: 30 2259
SfcI C'TryA_G
Cuts at: 0 563 1271 2289
Size: 563 708 1018
Fragments arranged by size:
1018 708 563
SfoI GGC'GCC
Cuts at: 0 51 2289
Size: 51 2238
SmaI CCC'GGG
Cuts at: 0 111 129 2289
Size: 111 18 2160
Fragments arranged by size:
2160 111 18
SphI G_CATG'C
Cuts at: 0 330 1548 2289
Size: 330 1218 741
Fragments arranged by size:
1218 741 330
StyI C'CwwG_G
Cuts at: 0 1211 1482 2289
Size: 1211 271 807
Fragments arranged by size:
1211 807 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 926 1360 1434 1892 2289
Size: 926 434 74 458 397
Fragments arranged by size:
926 458 434 397 74
TatI w'GTAC_w
Cuts at: 0 794 959 2289
Size: 794 165 1330
Fragments arranged by size:
1330 794 165
Tth111I GACn'n_nGTC
Cuts at: 0 187 1282 2151 2289
Size: 187 1095 869 138
Fragments arranged by size:
1095 869 187 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1486 1849 2289
Size: 1486 363 440
Fragments arranged by size:
1486 440 363
XmaI C'CCGG_G
Cuts at: 0 109 127 2289
Size: 109 18 2162
Fragments arranged by size:
2162 109 18
XmnI GAAnn'nnTTC
Cuts at: 0 425 2289
Size: 425 1864
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApaLI ApoI AseI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI
BsaHI BsaWI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BssHII BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HindIII KasI MmeI
MslI MspA1I NarI NcoI NheI NspI PfoI PpuMI
PshAI PsiI PvuII SacI SacII SfcI SfoI SmaI
SphI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI AscI AsiSI AvrII BaeI
BamHI BciVI BfrBI BglII BmgBI BpuEI BsaAI BsaBI
BsaXI BseYI BsiEI BsiWI BspEI BspHI BsrFI BsrGI
BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EarI EciI EcoNI FseI FspI FspAI HincII
HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TstI XbaI XhoI ZraI