Restriction map for
pF1KSDA0441
(Linear) (Six-base) MAPSORT of: KIAA0441.seq Check: 403 from: 1 to: 2091
KIAA0441.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1060 2018 2091
Size: 1060 958 73
Fragments arranged by size:
1060 958 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1538 1594 1976 2091
Size: 1330 208 56 382 115
Fragments arranged by size:
1330 382 208 115 56
AleI CACnn'nnGTG
Cuts at: 0 1286 2091
Size: 1286 805
AlwNI CAG_nnn'CTG
Cuts at: 0 2010 2091
Size: 2010 81
ApaI G_GGCC'C
Cuts at: 0 150 1886 2091
Size: 150 1736 205
Fragments arranged by size:
1736 205 150
ApaLI G'TGCA_C
Cuts at: 0 1903 2091
Size: 1903 188
ApoI r'AATT_y
Cuts at: 0 274 1240 1529 1652 1809 2091
Size: 274 966 289 123 157 282
Fragments arranged by size:
966 289 282 274 157 123
AvaI C'yCGr_G
Cuts at: 0 1638 2091
Size: 1638 453
BanI G'GyrC_C
Cuts at: 0 430 2091
Size: 430 1661
BanII G_rGCy'C
Cuts at: 0 150 1886 1973 2091
Size: 150 1736 87 118
Fragments arranged by size:
1736 150 118 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 495 2091
Size: 495 1596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 2091
Size: 27 2064
BciVI GTATCCnnnnn_n'
Cuts at: 0 274 2091
Size: 274 1817
BclI T'GATC_A
Cuts at: 0 1140 1910 2091
Size: 1140 770 181
Fragments arranged by size:
1140 770 181
BglI GCCn_nnn'nGGC
Cuts at: 0 27 869 2091
Size: 27 842 1222
Fragments arranged by size:
1222 842 27
BlpI GC'TnA_GC
Cuts at: 0 2003 2091
Size: 2003 88
Bme1580I G_kGCm'C
Cuts at: 0 150 435 1886 1907 2060 2091
Size: 150 285 1451 21 153 31
Fragments arranged by size:
1451 285 153 150 31 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 430 2091
Size: 430 1661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1117 1404 2091
Size: 887 230 287 687
Fragments arranged by size:
887 687 287 230
BsaAI yAC'GTr
Cuts at: 0 1908 2055 2091
Size: 1908 147 36
Fragments arranged by size:
1908 147 36
BsaWI w'CCGG_w
Cuts at: 0 1215 2091
Size: 1215 876
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 697 954 1385 1981 2091
Size: 532 165 257 431 596 110
Fragments arranged by size:
596 532 431 257 165 110
BseYI C'CCAG_C
Cuts at: 0 2060 2091
Size: 2060 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1083 1096 2091
Size: 1038 45 13 995
Fragments arranged by size:
1038 995 45 13
BsiEI CG_ry'CG
Cuts at: 0 1045 2091
Size: 1045 1046
BsiHKAI G_wGCw'C
Cuts at: 0 1103 1907 1973 2091
Size: 1103 804 66 118
Fragments arranged by size:
1103 804 118 66
BsmI GAATG_Cn'
Cuts at: 0 1225 1442 2091
Size: 1225 217 649
Fragments arranged by size:
1225 649 217
Bsp1286I G_dGCh'C
Cuts at: 0 150 435 1103 1886 1907 1973 2060 2091
Size: 150 285 668 783 21 66 87 31
Fragments arranged by size:
783 668 285 150 87 66 31 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1060 2018 2091
Size: 1060 958 73
Fragments arranged by size:
1060 958 73
BsrBI CCG'CTC
Cuts at: 0 1041 2091
Size: 1041 1050
BsrDI GCAATG_nn'
Cuts at: 0 508 2091
Size: 508 1583
BsrGI T'GTAC_A
Cuts at: 0 39 135 1677 2091
Size: 39 96 1542 414
Fragments arranged by size:
1542 414 96 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1061 1092 1229 2091
Size: 1061 31 137 862
Fragments arranged by size:
1061 862 137 31
BstYI r'GATC_y
Cuts at: 0 778 842 1995 2091
Size: 778 64 1153 96
Fragments arranged by size:
1153 778 96 64
Bsu36I CC'TnA_GG
Cuts at: 0 875 2091
Size: 875 1216
BtgI C'CryG_G
Cuts at: 0 825 2091
Size: 825 1266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1219 2091
Size: 1219 872
BtsI GCAGTG_nn'
Cuts at: 0 422 1090 1437 2091
Size: 422 668 347 654
Fragments arranged by size:
668 654 422 347
DraIII CAC_nnn'GTG
Cuts at: 0 1061 2055 2091
Size: 1061 994 36
Fragments arranged by size:
1061 994 36
EaeI y'GGCC_r
Cuts at: 0 71 216 735 1042 2076 2091
Size: 71 145 519 307 1034 15
Fragments arranged by size:
1034 519 307 145 71 15
EagI C'GGCC_G
Cuts at: 0 1042 2091
Size: 1042 1049
EarI CTCTTCn'nnn_
Cuts at: 0 197 1349 1586 2091
Size: 197 1152 237 505
Fragments arranged by size:
1152 505 237 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2024 2091
Size: 2024 67
EcoICRI GAG'CTC
Cuts at: 0 1971 2091
Size: 1971 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1117 1330 1404 1538 1594 1976 2091
Size: 887 230 213 74 134 56 382 115
Fragments arranged by size:
887 382 230 213 134 115 74 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 2091
Size: 364 1727
EcoO109I rG'GnC_Cy
Cuts at: 0 871 1882 1883 2091
Size: 871 1011 1 208
Fragments arranged by size:
1011 871 208 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 178 1539 2091
Size: 117 61 1361 552
Fragments arranged by size:
1361 552 117 61
HaeII r_GCGC'y
Cuts at: 0 864 2091
Size: 864 1227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 2091
Size: 508 32 1551
Fragments arranged by size:
1551 508 32
MscI TGG'CCA
Cuts at: 0 73 737 2078 2091
Size: 73 664 1341 13
Fragments arranged by size:
1341 664 73 13
MslI CAynn'nnrTG
Cuts at: 0 229 302 1024 1249 1286 1943 2091
Size: 229 73 722 225 37 657 148
Fragments arranged by size:
722 657 229 225 148 73 37
MspA1I CmG'CkG
Cuts at: 0 884 2016 2091
Size: 884 1132 75
Fragments arranged by size:
1132 884 75
NcoI C'CATG_G
Cuts at: 0 825 2091
Size: 825 1266
NspI r_CATG'y
Cuts at: 0 1552 2091
Size: 1552 539
PmlI CAC'GTG
Cuts at: 0 1908 2055 2091
Size: 1908 147 36
Fragments arranged by size:
1908 147 36
PshAI GACnn'nnGTC
Cuts at: 0 1125 2091
Size: 1125 966
PspOMI G'GGCC_C
Cuts at: 0 146 1882 2091
Size: 146 1736 209
Fragments arranged by size:
1736 209 146
PstI C_TGCA'G
Cuts at: 0 540 1734 1758 1808 2015 2091
Size: 540 1194 24 50 207 76
Fragments arranged by size:
1194 540 207 76 50 24
PvuII CAG'CTG
Cuts at: 0 2016 2091
Size: 2016 75
SacI G_AGCT'C
Cuts at: 0 1973 2091
Size: 1973 118
ScaI AGT'ACT
Cuts at: 0 1989 2091
Size: 1989 102
SexAI A'CCwGG_T
Cuts at: 0 359 2091
Size: 359 1732
SfcI C'TryA_G
Cuts at: 0 536 1009 1615 1730 1754 1804 2011 2091
Size: 536 473 606 115 24 50 207 80
Fragments arranged by size:
606 536 473 207 115 80 50 24
SphI G_CATG'C
Cuts at: 0 1552 2091
Size: 1552 539
SspI AAT'ATT
Cuts at: 0 1156 1605 2091
Size: 1156 449 486
Fragments arranged by size:
1156 486 449
StyI C'CwwG_G
Cuts at: 0 825 1922 2091
Size: 825 1097 169
Fragments arranged by size:
1097 825 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 742 2058 2091
Size: 742 1316 33
Fragments arranged by size:
1316 742 33
TatI w'GTAC_w
Cuts at: 0 39 135 313 615 911 1677 1987 2091
Size: 39 96 178 302 296 766 310 104
Fragments arranged by size:
766 310 302 296 178 104 96 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 1929 2091
Size: 231 1698 162
Fragments arranged by size:
1698 231 162
XmnI GAAnn'nnTTC
Cuts at: 0 100 1465 1847 1940 2091
Size: 100 1365 382 93 151
Fragments arranged by size:
1365 382 151 100 93
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbsI BcgI BciVI BclI BglI BlpI
Bme1580I BmrI BpmI BsaAI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I MscI MslI MspA1I NcoI NspI PmlI
PshAI PspOMI PstI PvuII SacI ScaI SexAI SfcI
SphI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BfrBI BglII BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BsiWI BsmBI
BspEI BspHI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI