Restriction map for
pF1KA0437
(Linear) (Six-base) MAPSORT of: KIAA0437.seq Check: 7011 from: 1 to: 4788
KIAA0437.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3356 4788
Size: 3356 1432
AccI GT'mk_AC
Cuts at: 0 3694 4788
Size: 3694 1094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 916 2089 3014 4204 4321 4788
Size: 328 588 1173 925 1190 117 467
Fragments arranged by size:
1190 1173 925 588 467 328 117
AflII C'TTAA_G
Cuts at: 0 18 4788
Size: 18 4770
AflIII A'CryG_T
Cuts at: 0 815 3836 4788
Size: 815 3021 952
Fragments arranged by size:
3021 952 815
AgeI A'CCGG_T
Cuts at: 0 604 4788
Size: 604 4184
AhdI GACnn_n'nnGTC
Cuts at: 0 3039 3690 4788
Size: 3039 651 1098
Fragments arranged by size:
3039 1098 651
AleI CACnn'nnGTG
Cuts at: 0 2523 4788
Size: 2523 2265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4456 4788
Size: 4456 332
AlwNI CAG_nnn'CTG
Cuts at: 0 130 721 2859 4788
Size: 130 591 2138 1929
Fragments arranged by size:
2138 1929 591 130
ApaLI G'TGCA_C
Cuts at: 0 1524 4788
Size: 1524 3264
ApoI r'AATT_y
Cuts at: 0 268 354 1573 1939 4788
Size: 268 86 1219 366 2849
Fragments arranged by size:
2849 1219 366 268 86
AseI AT'TA_AT
Cuts at: 0 2138 2963 4788
Size: 2138 825 1825
Fragments arranged by size:
2138 1825 825
AvaI C'yCGr_G
Cuts at: 0 4547 4643 4760 4788
Size: 4547 96 117 28
Fragments arranged by size:
4547 117 96 28
AvrII C'CTAG_G
Cuts at: 0 3489 4788
Size: 3489 1299
BamHI G'GATC_C
Cuts at: 0 140 1233 1380 3485 4788
Size: 140 1093 147 2105 1303
Fragments arranged by size:
2105 1303 1093 147 140
BanI G'GyrC_C
Cuts at: 0 1221 2985 3093 4554 4788
Size: 1221 1764 108 1461 234
Fragments arranged by size:
1764 1461 1221 234 108
BanII G_rGCy'C
Cuts at: 0 767 974 1673 2374 2656 3590 4477 4493
Size: 767 207 699 701 282 934 887 16
Cuts at: 4493 4788
Size: 295
Fragments arranged by size:
934 887 767 701 699 295 282 207
16
BbeI G_GCGC'C
Cuts at: 0 4558 4788
Size: 4558 230
BbsI GAAGACnn'nnnn_
Cuts at: 0 1987 2014 2303 3485 4788
Size: 1987 27 289 1182 1303
Fragments arranged by size:
1987 1303 1182 289 27
BbvCI CC'TCA_GC
Cuts at: 0 69 4788
Size: 69 4719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4142 4176 4326 4360 4788
Size: 4142 34 150 34 428
Fragments arranged by size:
4142 428 150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1343 4788
Size: 1343 3445
BclI T'GATC_A
Cuts at: 0 1736 2970 4788
Size: 1736 1234 1818
Fragments arranged by size:
1818 1736 1234
BfrBI ATG'CAT
Cuts at: 0 4433 4788
Size: 4433 355
BglI GCCn_nnn'nGGC
Cuts at: 0 1135 4552 4788
Size: 1135 3417 236
Fragments arranged by size:
3417 1135 236
BglII A'GATC_T
Cuts at: 0 846 2039 4788
Size: 846 1193 2749
Fragments arranged by size:
2749 1193 846
BlpI GC'TnA_GC
Cuts at: 0 3320 4682 4788
Size: 3320 1362 106
Fragments arranged by size:
3320 1362 106
Bme1580I G_kGCm'C
Cuts at: 0 1131 1528 4067 4788
Size: 1131 397 2539 721
Fragments arranged by size:
2539 1131 721 397
BmgBI CAC'GTC
Cuts at: 0 3837 3882 4788
Size: 3837 45 906
Fragments arranged by size:
3837 906 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 2134 3122 4120 4380 4788
Size: 2134 988 998 260 408
Fragments arranged by size:
2134 998 988 408 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1443 2553 2670 4788
Size: 1443 1110 117 2118
Fragments arranged by size:
2118 1443 1110 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1687 1727 2407 2836 2890 3528 3980
Size: 107 1580 40 680 429 54 638 452
Cuts at: 3980 4386 4507 4788
Size: 406 121 281
Fragments arranged by size:
1580 680 638 452 429 406 281 121
107 54 40
Bpu10I CC'TnA_GC
Cuts at: 0 69 192 2368 3556 4471 4788
Size: 69 123 2176 1188 915 317
Fragments arranged by size:
2176 1188 915 317 123 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1173 1999 2782 3771 4788
Size: 781 392 826 783 989 1017
Fragments arranged by size:
1017 989 826 783 781 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 2083 4400 4788
Size: 69 2014 2317 388
Fragments arranged by size:
2317 2014 388 69
BsaAI yAC'GTr
Cuts at: 0 818 4287 4788
Size: 818 3469 501
Fragments arranged by size:
3469 818 501
BsaBI GATnn'nnATC
Cuts at: 0 388 394 1207 4788
Size: 388 6 813 3581
Fragments arranged by size:
3581 813 388 6
BsaHI Gr'CG_yC
Cuts at: 0 2308 3713 4365 4555 4788
Size: 2308 1405 652 190 233
Fragments arranged by size:
2308 1405 652 233 190
BsaWI w'CCGG_w
Cuts at: 0 604 1199 2912 3310 3482 4788
Size: 604 595 1713 398 172 1306
Fragments arranged by size:
1713 1306 604 595 398 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1660 1690 3277 3307 4788
Size: 1660 30 1587 30 1481
Fragments arranged by size:
1660 1587 1481 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 187 190 478 971 2599 2887 3915
Size: 57 130 3 288 493 1628 288 1028
Cuts at: 3915 3975 4167 4744 4788
Size: 60 192 577 44
Fragments arranged by size:
1628 1028 577 493 288 288 192 130
60 57 44 3
BseYI C'CCAG_C
Cuts at: 0 81 904 1898 2596 2947 3207 3716 4444
Size: 81 823 994 698 351 260 509 728
Cuts at: 4444 4465 4788
Size: 21 323
Fragments arranged by size:
994 823 728 698 509 351 323 260
81 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 827 3335 4788
Size: 119 708 2508 1453
Fragments arranged by size:
2508 1453 708 119
BsiHKAI G_wGCw'C
Cuts at: 0 1528 2439 2656 3590 4788
Size: 1528 911 217 934 1198
Fragments arranged by size:
1528 1198 934 911 217
BsmI GAATG_Cn'
Cuts at: 0 1074 3161 4788
Size: 1074 2087 1627
Fragments arranged by size:
2087 1627 1074
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1789 2581 4788
Size: 1789 792 2207
Fragments arranged by size:
2207 1789 792
Bsp1286I G_dGCh'C
Cuts at: 0 767 974 1131 1528 1673 2374 2439 2656
Size: 767 207 157 397 145 701 65 217
Cuts at: 2656 3590 4067 4477 4493 4788
Size: 934 477 410 16 295
Fragments arranged by size:
934 767 701 477 410 397 295 217
207 157 145 65 16
BspEI T'CCGG_A
Cuts at: 0 1199 4788
Size: 1199 3589
BspHI T'CATG_A
Cuts at: 0 1508 1803 3254 4788
Size: 1508 295 1451 1534
Fragments arranged by size:
1534 1508 1451 295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3356 4448 4788
Size: 3356 1092 340
Fragments arranged by size:
3356 1092 340
BsrBI CCG'CTC
Cuts at: 0 757 3564 4681 4788
Size: 757 2807 1117 107
Fragments arranged by size:
2807 1117 757 107
BsrFI r'CCGG_y
Cuts at: 0 60 604 2738 2750 3649 4416 4788
Size: 60 544 2134 12 899 767 372
Fragments arranged by size:
2134 899 767 544 372 60 12
BssSI C'ACGA_G
Cuts at: 0 2932 3816 4788
Size: 2932 884 972
Fragments arranged by size:
2932 972 884
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1126 1782 4788
Size: 1126 656 3006
Fragments arranged by size:
3006 1126 656
BstBI TT'CG_AA
Cuts at: 0 3859 4788
Size: 3859 929
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2502 4788
Size: 2502 2286
BstYI r'GATC_y
Cuts at: 0 140 749 846 1233 1380 2039 3485 4256
Size: 140 609 97 387 147 659 1446 771
Cuts at: 4256 4295 4788
Size: 39 493
Fragments arranged by size:
1446 771 659 609 493 387 147 140
97 39
BtgI C'CryG_G
Cuts at: 0 767 1489 1502 4788
Size: 767 722 13 3286
Fragments arranged by size:
3286 767 722 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2858 4788
Size: 2858 1930
BtsI GCAGTG_nn'
Cuts at: 0 810 1338 3420 3762 3801 4043 4788
Size: 810 528 2082 342 39 242 745
Fragments arranged by size:
2082 810 745 528 342 242 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3492 3527 4788
Size: 3492 35 1261
Fragments arranged by size:
3492 1261 35
EaeI y'GGCC_r
Cuts at: 0 1643 2504 2745 3651 4517 4788
Size: 1643 861 241 906 866 271
Fragments arranged by size:
1643 906 866 861 271 241
EarI CTCTTCn'nnn_
Cuts at: 0 254 2444 2644 4183 4348 4721 4788
Size: 254 2190 200 1539 165 373 67
Fragments arranged by size:
2190 1539 373 254 200 165 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 167 4664 4788
Size: 167 4497 124
Fragments arranged by size:
4497 167 124
EcoICRI GAG'CTC
Cuts at: 0 2654 3588 4788
Size: 2654 934 1200
Fragments arranged by size:
2654 1200 934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 328 916 1687 1727 2089 2407 2836
Size: 107 221 588 771 40 362 318 429
Cuts at: 2836 2890 3014 3528 3980 4204 4321 4386 4507
Size: 54 124 514 452 224 117 65 121
Cuts at: 4507 4788
Size: 281
Fragments arranged by size:
771 588 514 452 429 362 318 281
224 221 124 121 117 107 65 54
40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 4720 4788
Size: 592 4128 68
Fragments arranged by size:
4128 592 68
EcoO109I rG'GnC_Cy
Cuts at: 0 127 551 3355 3494 4674 4779 4788
Size: 127 424 2804 139 1180 105 9
Fragments arranged by size:
2804 1180 424 139 127 105 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1613 3690 4788
Size: 1613 2077 1098
Fragments arranged by size:
2077 1613 1098
HaeII r_GCGC'y
Cuts at: 0 4558 4788
Size: 4558 230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 741 773 1405 1437 1660 1692
Size: 199 32 510 32 632 32 223 32
Cuts at: 1692 2674 2706 3025 3057 3676 3708 3993 4025
Size: 982 32 319 32 619 32 285 32
Cuts at: 4025 4384 4416 4788
Size: 359 32 372
Fragments arranged by size:
982 632 619 510 372 359 319 285
223 199 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1344 4101 4788
Size: 1344 2757 687
Fragments arranged by size:
2757 1344 687
HindIII A'AGCT_T
Cuts at: 0 3659 4788
Size: 3659 1129
HpaI GTT'AAC
Cuts at: 0 1344 4788
Size: 1344 3444
KasI G'GCGC_C
Cuts at: 0 4554 4788
Size: 4554 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 242 1261 1382 1543 1935 1952 2312
Size: 236 6 1019 121 161 392 17 360
Cuts at: 2312 2467 2744 3734 4142 4788
Size: 155 277 990 408 646
Fragments arranged by size:
1019 990 646 408 392 360 277 236
161 155 121 17 6
MscI TGG'CCA
Cuts at: 0 1645 2506 2747 4519 4788
Size: 1645 861 241 1772 269
Fragments arranged by size:
1772 1645 861 269 241
MslI CAynn'nnrTG
Cuts at: 0 1507 2523 3169 3304 4072 4513 4540 4788
Size: 1507 1016 646 135 768 441 27 248
Fragments arranged by size:
1507 1016 768 646 441 248 135 27
MspA1I CmG'CkG
Cuts at: 0 85 683 1491 2606 3594 4479 4788
Size: 85 598 808 1115 988 885 309
Fragments arranged by size:
1115 988 885 808 598 309 85
NaeI GCC'GGC
Cuts at: 0 3651 4788
Size: 3651 1137
NarI GG'CG_CC
Cuts at: 0 4555 4788
Size: 4555 233
NcoI C'CATG_G
Cuts at: 0 1502 4788
Size: 1502 3286
NgoMIV G'CCGG_C
Cuts at: 0 3649 4788
Size: 3649 1139
NsiI A_TGCA'T
Cuts at: 0 4435 4788
Size: 4435 353
NspI r_CATG'y
Cuts at: 0 3384 4788
Size: 3384 1404
PasI CC'CwG_GG
Cuts at: 0 789 2098 4788
Size: 789 1309 2690
Fragments arranged by size:
2690 1309 789
PflMI CCAn_nnn'nTGG
Cuts at: 0 130 721 2859 4788
Size: 130 591 2138 1929
Fragments arranged by size:
2138 1929 591 130
PfoI T'CCnGG_A
Cuts at: 0 123 4788
Size: 123 4665
PmlI CAC'GTG
Cuts at: 0 818 4287 4788
Size: 818 3469 501
Fragments arranged by size:
3469 818 501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 4042 4074 4788
Size: 98 32 3912 32 714
Fragments arranged by size:
3912 714 98 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 127 3355 3494 4779 4788
Size: 127 3228 139 1285 9
Fragments arranged by size:
3228 1285 139 127 9
PshAI GACnn'nnGTC
Cuts at: 0 1190 4788
Size: 1190 3598
PstI C_TGCA'G
Cuts at: 0 3353 4155 4319 4788
Size: 3353 802 164 469
Fragments arranged by size:
3353 802 469 164
PvuII CAG'CTG
Cuts at: 0 85 4788
Size: 85 4703
SacI G_AGCT'C
Cuts at: 0 2656 3590 4788
Size: 2656 934 1198
Fragments arranged by size:
2656 1198 934
SacII CC_GC'GG
Cuts at: 0 1492 4788
Size: 1492 3296
SanDI GG'GwC_CC
Cuts at: 0 3494 4788
Size: 3494 1294
SapI GCTCTTCn'nnn_
Cuts at: 0 254 2444 2644 4788
Size: 254 2190 200 2144
Fragments arranged by size:
2190 2144 254 200
SbfI CC_TGCA'GG
Cuts at: 0 3353 4788
Size: 3353 1435
SfcI C'TryA_G
Cuts at: 0 3349 4151 4315 4788
Size: 3349 802 164 473
Fragments arranged by size:
3349 802 473 164
SfoI GGC'GCC
Cuts at: 0 4556 4788
Size: 4556 232
SgrAI Cr'CCGG_yG
Cuts at: 0 604 4788
Size: 604 4184
SmaI CCC'GGG
Cuts at: 0 4549 4788
Size: 4549 239
SmlI C'TyrA_G
Cuts at: 0 18 760 1188 2014 2797 3750 4788
Size: 18 742 428 826 783 953 1038
Fragments arranged by size:
1038 953 826 783 742 428 18
StuI AGG'CCT
Cuts at: 0 2250 3063 4788
Size: 2250 813 1725
Fragments arranged by size:
2250 1725 813
StyI C'CwwG_G
Cuts at: 0 938 1307 1502 3186 3372 3404 3489 3734
Size: 938 369 195 1684 186 32 85 245
Cuts at: 3734 4788
Size: 1054
Fragments arranged by size:
1684 1054 938 369 245 195 186 85
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 918 1515 2345 2508 4399 4788
Size: 228 690 597 830 163 1891 389
Fragments arranged by size:
1891 830 690 597 389 228 163
TatI w'GTAC_w
Cuts at: 0 3342 4788
Size: 3342 1446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 3266 3277 3298 3309 4788
Size: 1525 32 1709 11 21 11 1479
Fragments arranged by size:
1709 1525 1479 32 21 11 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1055 1275 4275 4788
Size: 1055 220 3000 513
Fragments arranged by size:
3000 1055 513 220
XmaI C'CCGG_G
Cuts at: 0 4547 4788
Size: 4547 241
XmnI GAAnn'nnTTC
Cuts at: 0 703 4192 4788
Size: 703 3489 596
Fragments arranged by size:
3489 703 596
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII HpaI KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PstI PvuII SacI
SacII SanDI SapI SbfI SfcI SfoI SgrAI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AloI ApaI AscI AsiSI
BaeI BmtI BsiEI BsiWI BsrDI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EcoRI
EcoRV FseI FspI FspAI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PciI PmeI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI ScaI SexAI SfiI
SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI