Restriction map for
pF1KSDA0436
(Linear) (Six-base) MAPSORT of: KIAA0436.seq Check: 7413 from: 1 to: 1914
KIAA0436.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
Acc65I G'GTAC_C
Cuts at: 0 1674 1914
Size: 1674 240
AclI AA'CG_TT
Cuts at: 0 1596 1914
Size: 1596 318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 290 578 841 1914
Size: 281 9 288 263 1073
Fragments arranged by size:
1073 288 281 263 9
AflII C'TTAA_G
Cuts at: 0 184 1894 1914
Size: 184 1710 20
Fragments arranged by size:
1710 184 20
AflIII A'CryG_T
Cuts at: 0 791 1161 1914
Size: 791 370 753
Fragments arranged by size:
791 753 370
AlwNI CAG_nnn'CTG
Cuts at: 0 718 1914
Size: 718 1196
ApoI r'AATT_y
Cuts at: 0 694 924 1197 1843 1909 1914
Size: 694 230 273 646 66 5
Fragments arranged by size:
694 646 273 230 66 5
BanI G'GyrC_C
Cuts at: 0 1479 1674 1914
Size: 1479 195 240
Fragments arranged by size:
1479 240 195
BanII G_rGCy'C
Cuts at: 0 586 1914
Size: 586 1328
BbsI GAAGACnn'nnnn_
Cuts at: 0 1040 1914
Size: 1040 874
BbvCI CC'TCA_GC
Cuts at: 0 1625 1914
Size: 1625 289
BciVI GTATCCnnnnn_n'
Cuts at: 0 912 1914
Size: 912 1002
BfrBI ATG'CAT
Cuts at: 0 7 1914
Size: 7 1907
BglI GCCn_nnn'nGGC
Cuts at: 0 1212 1914
Size: 1212 702
BlpI GC'TnA_GC
Cuts at: 0 298 1914
Size: 298 1616
BmrI ACTGGGnnnn_n'
Cuts at: 0 1034 1914
Size: 1034 880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1267 1433 1811 1914
Size: 209 1058 166 378 103
Fragments arranged by size:
1058 378 209 166 103
Bpu10I CC'TnA_GC
Cuts at: 0 580 1625 1736 1914
Size: 580 1045 111 178
Fragments arranged by size:
1045 580 178 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1696 1914
Size: 1696 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 884 1914
Size: 884 1030
Bsp1286I G_dGCh'C
Cuts at: 0 586 1914
Size: 586 1328
BspHI T'CATG_A
Cuts at: 0 414 1914
Size: 414 1500
BsrBI CCG'CTC
Cuts at: 0 1465 1914
Size: 1465 449
BstYI r'GATC_y
Cuts at: 0 1890 1914
Size: 1890 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1707 1914
Size: 1707 207
EaeI y'GGCC_r
Cuts at: 0 1299 1362 1914
Size: 1299 63 552
Fragments arranged by size:
1299 552 63
EarI CTCTTCn'nnn_
Cuts at: 0 757 1914
Size: 757 1157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 281 290 578 841 1267 1433 1811
Size: 209 72 9 288 263 426 166 378
Cuts at: 1811 1914
Size: 103
Fragments arranged by size:
426 378 288 263 209 166 103 72
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 396 1682 1852 1914
Size: 396 1286 170 62
Fragments arranged by size:
1286 396 170 62
EcoO109I rG'GnC_Cy
Cuts at: 0 591 627 1218 1914
Size: 591 36 591 696
Fragments arranged by size:
696 591 591 36
HindIII A'AGCT_T
Cuts at: 0 320 1914
Size: 320 1594
KpnI G_GTAC'C
Cuts at: 0 1678 1914
Size: 1678 236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1914
Size: 660 1254
MscI TGG'CCA
Cuts at: 0 1364 1914
Size: 1364 550
MslI CAynn'nnrTG
Cuts at: 0 67 650 1166 1295 1507 1914
Size: 67 583 516 129 212 407
Fragments arranged by size:
583 516 407 212 129 67
MspA1I CmG'CkG
Cuts at: 0 715 1914
Size: 715 1199
NdeI CA'TA_TG
Cuts at: 0 1656 1914
Size: 1656 258
NsiI A_TGCA'T
Cuts at: 0 9 1914
Size: 9 1905
NspI r_CATG'y
Cuts at: 0 795 1165 1735 1914
Size: 795 370 570 179
Fragments arranged by size:
795 570 370 179
PciI A'CATG_T
Cuts at: 0 791 1161 1914
Size: 791 370 753
Fragments arranged by size:
791 753 370
PpuMI rG'GwC_Cy
Cuts at: 0 591 627 1218 1914
Size: 591 36 591 696
Fragments arranged by size:
696 591 591 36
PsiI TTA'TAA
Cuts at: 0 292 1914
Size: 292 1622
SanDI GG'GwC_CC
Cuts at: 0 591 1914
Size: 591 1323
ScaI AGT'ACT
Cuts at: 0 602 1914
Size: 602 1312
SfcI C'TryA_G
Cuts at: 0 689 1914
Size: 689 1225
SmlI C'TyrA_G
Cuts at: 0 184 1711 1894 1914
Size: 184 1527 183 20
Fragments arranged by size:
1527 184 183 20
SpeI A'CTAG_T
Cuts at: 0 1051 1914
Size: 1051 863
SphI G_CATG'C
Cuts at: 0 1735 1914
Size: 1735 179
SspI AAT'ATT
Cuts at: 0 537 982 1617 1770 1914
Size: 537 445 635 153 144
Fragments arranged by size:
635 537 445 153 144
StuI AGG'CCT
Cuts at: 0 1206 1280 1914
Size: 1206 74 634
Fragments arranged by size:
1206 634 74
StyI C'CwwG_G
Cuts at: 0 586 895 1365 1914
Size: 586 309 470 549
Fragments arranged by size:
586 549 470 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 866 1914
Size: 866 1048
TatI w'GTAC_w
Cuts at: 0 600 1914
Size: 600 1314
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1082 1114 1506 1538 1914
Size: 1082 32 392 32 376
Fragments arranged by size:
1082 392 376 32 32
XbaI T'CTAG_A
Cuts at: 0 1068 1776 1914
Size: 1068 708 138
Fragments arranged by size:
1068 708 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1261 1914
Size: 1261 653
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AlwNI ApoI BanI
BanII BbsI BbvCI BciVI BfrBI BglI BlpI BmrI
BpmI Bpu10I BpuEI BsaI Bsp1286I BspHI BsrBI BstYI
BtgZI EaeI EarI Eco57MI EcoNI EcoO109I HindIII KpnI
MmeI MscI MslI MspA1I NdeI NsiI NspI PciI
PpuMI PsiI SanDI ScaI SfcI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BclI BglII Bme1580I BmgBI
BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HpaI
KasI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI Tth111I
XhoI XmaI XmnI ZraI