Restriction map for
pF1KA0433
(Linear) (Six-base) MAPSORT of: KIAA0433.seq Check: 3835 from: 1 to: 3729
KIAA0433.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3564 3729
Size: 3564 165
AccI GT'mk_AC
Cuts at: 0 3550 3729
Size: 3550 179
Acc65I G'GTAC_C
Cuts at: 0 2962 3729
Size: 2962 767
AclI AA'CG_TT
Cuts at: 0 2573 3729
Size: 2573 1156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 797 957 1112 1572 1589 1864 1925
Size: 449 348 160 155 460 17 275 61
Cuts at: 1925 2353 3729
Size: 428 1376
Fragments arranged by size:
1376 460 449 428 348 275 160 155
61 17
AflII C'TTAA_G
Cuts at: 0 1384 3729
Size: 1384 2345
AflIII A'CryG_T
Cuts at: 0 1851 3729
Size: 1851 1878
AleI CACnn'nnGTG
Cuts at: 0 3199 3729
Size: 3199 530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3217 3729
Size: 3217 512
ApoI r'AATT_y
Cuts at: 0 185 987 1216 2354 2712 3433 3488 3729
Size: 185 802 229 1138 358 721 55 241
Fragments arranged by size:
1138 802 721 358 241 229 185 55
AseI AT'TA_AT
Cuts at: 0 874 3729
Size: 874 2855
AvaI C'yCGr_G
Cuts at: 0 3205 3729
Size: 3205 524
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 722 755 3729
Size: 722 33 2974
Fragments arranged by size:
2974 722 33
BamHI G'GATC_C
Cuts at: 0 2625 3729
Size: 2625 1104
BanI G'GyrC_C
Cuts at: 0 2515 2962 3372 3729
Size: 2515 447 410 357
Fragments arranged by size:
2515 447 410 357
BanII G_rGCy'C
Cuts at: 0 2588 2932 3729
Size: 2588 344 797
Fragments arranged by size:
2588 797 344
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 1987 2711 2911 3012 3484 3729
Size: 95 1892 724 200 101 472 245
Fragments arranged by size:
1892 724 472 245 200 101 95
BciVI GTATCCnnnnn_n'
Cuts at: 0 1610 3729
Size: 1610 2119
BfrBI ATG'CAT
Cuts at: 0 1959 3729
Size: 1959 1770
BglII A'GATC_T
Cuts at: 0 2896 3729
Size: 2896 833
BmrI ACTGGGnnnn_n'
Cuts at: 0 3005 3725 3729
Size: 3005 720 4
Fragments arranged by size:
3005 720 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 776 1625 2335 3729
Size: 653 123 849 710 1394
Fragments arranged by size:
1394 849 710 653 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 2760 3176 3729
Size: 2760 416 553
Fragments arranged by size:
2760 553 416
BsaAI yAC'GTr
Cuts at: 0 2104 3548 3729
Size: 2104 1444 181
Fragments arranged by size:
2104 1444 181
BsaBI GATnn'nnATC
Cuts at: 0 1251 2608 3729
Size: 1251 1357 1121
Fragments arranged by size:
1357 1251 1121
BsaHI Gr'CG_yC
Cuts at: 0 3000 3290 3729
Size: 3000 290 439
Fragments arranged by size:
3000 439 290
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 253 1498 2330 3729
Size: 112 141 1245 832 1399
Fragments arranged by size:
1399 1245 832 141 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 3659 3729
Size: 590 3069 70
Fragments arranged by size:
3069 590 70
BsiHKAI G_wGCw'C
Cuts at: 0 2588 3206 3598 3729
Size: 2588 618 392 131
Fragments arranged by size:
2588 618 392 131
Bsp1286I G_dGCh'C
Cuts at: 0 2588 2932 3206 3598 3729
Size: 2588 344 274 392 131
Fragments arranged by size:
2588 392 344 274 131
BspHI T'CATG_A
Cuts at: 0 1869 3729
Size: 1869 1860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3128 3564 3729
Size: 3128 436 165
Fragments arranged by size:
3128 436 165
BsrDI GCAATG_nn'
Cuts at: 0 684 2532 3446 3729
Size: 684 1848 914 283
Fragments arranged by size:
1848 914 684 283
BsrGI T'GTAC_A
Cuts at: 0 2484 3729
Size: 2484 1245
BssSI C'ACGA_G
Cuts at: 0 863 3729
Size: 863 2866
BstBI TT'CG_AA
Cuts at: 0 2254 3729
Size: 2254 1475
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2209 2988 3057 3729
Size: 2209 779 69 672
Fragments arranged by size:
2209 779 672 69
BstYI r'GATC_y
Cuts at: 0 197 2046 2625 2637 2896 3563 3729
Size: 197 1849 579 12 259 667 166
Fragments arranged by size:
1849 667 579 259 197 166 12
BstZ17I GTA'TAC
Cuts at: 0 3551 3729
Size: 3551 178
Bsu36I CC'TnA_GG
Cuts at: 0 2282 3729
Size: 2282 1447
BtgI C'CryG_G
Cuts at: 0 149 1069 3368 3472 3729
Size: 149 920 2299 104 257
Fragments arranged by size:
2299 920 257 149 104
DraI TTT'AAA
Cuts at: 0 210 2133 2570 2775 3419 3542 3729
Size: 210 1923 437 205 644 123 187
Fragments arranged by size:
1923 644 437 210 205 187 123
EarI CTCTTCn'nnn_
Cuts at: 0 230 704 1475 1491 1575 2918 3276 3404
Size: 230 474 771 16 84 1343 358 128
Cuts at: 3404 3729
Size: 325
Fragments arranged by size:
1343 771 474 358 325 230 128 84
16
EcoICRI GAG'CTC
Cuts at: 0 2586 3729
Size: 2586 1143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 653 776 797 957 1112 1572 1589
Size: 449 204 123 21 160 155 460 17
Cuts at: 1589 1625 1864 1925 2335 2353 3729
Size: 36 239 61 410 18 1376
Fragments arranged by size:
1376 460 449 410 239 204 160 155
123 61 36 21 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3164 3729
Size: 3164 565
EcoRV GAT'ATC
Cuts at: 0 1098 3729
Size: 1098 2631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 910 1777 3647 3729
Size: 910 867 1870 82
Fragments arranged by size:
1870 910 867 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1392 1424 1661 1693 1813 1827 1845 1859
Size: 1392 32 237 32 120 14 18 14
Cuts at: 1859 2038 2070 2629 2661 3729
Size: 179 32 559 32 1068
Fragments arranged by size:
1392 1068 559 237 179 120 32 32
32 32 18 14 14
HincII GTy'rAC
Cuts at: 0 1442 3729
Size: 1442 2287
HindIII A'AGCT_T
Cuts at: 0 1918 3729
Size: 1918 1811
KpnI G_GTAC'C
Cuts at: 0 2966 3729
Size: 2966 763
MfeI C'AATT_G
Cuts at: 0 1103 3729
Size: 1103 2626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 117 3729
Size: 6 111 3612
Fragments arranged by size:
3612 111 6
MslI CAynn'nnrTG
Cuts at: 0 83 3199 3729
Size: 83 3116 530
Fragments arranged by size:
3116 530 83
MspA1I CmG'CkG
Cuts at: 0 1723 3484 3729
Size: 1723 1761 245
Fragments arranged by size:
1761 1723 245
NcoI C'CATG_G
Cuts at: 0 149 1069 3368 3729
Size: 149 920 2299 361
Fragments arranged by size:
2299 920 361 149
NsiI A_TGCA'T
Cuts at: 0 1961 3729
Size: 1961 1768
PacI TTA_AT'TAA
Cuts at: 0 1967 3729
Size: 1967 1762
PflMI CCAn_nnn'nTGG
Cuts at: 0 3349 3729
Size: 3349 380
PfoI T'CCnGG_A
Cuts at: 0 51 1602 2682 3729
Size: 51 1551 1080 1047
Fragments arranged by size:
1551 1080 1047 51
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1496 1528 3729
Size: 1496 32 2201
Fragments arranged by size:
2201 1496 32
PstI C_TGCA'G
Cuts at: 0 1554 1722 3729
Size: 1554 168 2007
Fragments arranged by size:
2007 1554 168
PvuII CAG'CTG
Cuts at: 0 1723 3729
Size: 1723 2006
SacI G_AGCT'C
Cuts at: 0 2588 3729
Size: 2588 1141
SapI GCTCTTCn'nnn_
Cuts at: 0 1575 3729
Size: 1575 2154
SbfI CC_TGCA'GG
Cuts at: 0 1554 3729
Size: 1554 2175
SfcI C'TryA_G
Cuts at: 0 1550 1665 1718 3164 3232 3497 3729
Size: 1550 115 53 1446 68 265 232
Fragments arranged by size:
1550 1446 265 232 115 68 53
SmlI C'TyrA_G
Cuts at: 0 1384 3729
Size: 1384 2345
SnaBI TAC'GTA
Cuts at: 0 2104 3548 3729
Size: 2104 1444 181
Fragments arranged by size:
2104 1444 181
SspI AAT'ATT
Cuts at: 0 1032 3729
Size: 1032 2697
StyI C'CwwG_G
Cuts at: 0 149 1069 3368 3729
Size: 149 920 2299 361
Fragments arranged by size:
2299 920 361 149
SwaI ATTT'AAAT
Cuts at: 0 210 3729
Size: 210 3519
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1782 3356 3729
Size: 1782 1574 373
Fragments arranged by size:
1782 1574 373
TatI w'GTAC_w
Cuts at: 0 2335 2484 3729
Size: 2335 149 1245
Fragments arranged by size:
2335 1245 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2530 2562 3729
Size: 2530 32 1167
Fragments arranged by size:
2530 1167 32
XbaI T'CTAG_A
Cuts at: 0 2419 3390 3729
Size: 2419 971 339
Fragments arranged by size:
2419 971 339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 266 3729
Size: 266 3463
XmnI GAAnn'nnTTC
Cuts at: 0 144 536 1877 1938 2657 3398 3729
Size: 144 392 1341 61 719 741 331
Fragments arranged by size:
1341 741 719 392 331 144 61
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BciVI BfrBI BglII BmrI BpmI BsaI BsaAI
BsaBI BsaHI BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI DraI EarI EcoICRI Eco57MI EcoNI EcoRV FalI
Hin4I HincII HindIII KpnI MfeI MmeI MslI MspA1I
NcoI NsiI PacI PflMI PfoI PsrI PstI PvuII
SacI SapI SbfI SfcI SmlI SnaBI SspI StyI
SwaI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI AlwNI ApaI ApaLI
AscI AsiSI AvrII BbeI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaWI
BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BssHII BstAPI BstEII BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoO109I EcoRI FseI
FspI FspAI HaeII HpaI KasI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
StuI Tth111I XhoI XmaI ZraI