Restriction map for
pF1KSDA0416
(Linear) (Six-base) MAPSORT of: KIAA0416.seq Check: 7109 from: 1 to: 1548
KIAA0416.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 1548
Size: 234 1314
AflII C'TTAA_G
Cuts at: 0 331 345 1548
Size: 331 14 1203
Fragments arranged by size:
1203 331 14
AflIII A'CryG_T
Cuts at: 0 733 1430 1548
Size: 733 697 118
Fragments arranged by size:
733 697 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1548
Size: 992 32 524
Fragments arranged by size:
992 524 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1524 1548
Size: 1524 24
ApaI G_GGCC'C
Cuts at: 0 33 1548
Size: 33 1515
ApoI r'AATT_y
Cuts at: 0 287 408 1083 1548
Size: 287 121 675 465
Fragments arranged by size:
675 465 287 121
AseI AT'TA_AT
Cuts at: 0 340 610 659 1493 1548
Size: 340 270 49 834 55
Fragments arranged by size:
834 340 270 55 49
BanII G_rGCy'C
Cuts at: 0 33 187 227 455 1548
Size: 33 154 40 228 1093
Fragments arranged by size:
1093 228 154 40 33
BglII A'GATC_T
Cuts at: 0 884 1548
Size: 884 664
BlpI GC'TnA_GC
Cuts at: 0 682 1548
Size: 682 866
Bme1580I G_kGCm'C
Cuts at: 0 33 760 1548
Size: 33 727 788
Fragments arranged by size:
788 727 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 511 1548
Size: 511 1037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1314 1548
Size: 568 746 234
Fragments arranged by size:
746 568 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 925 1548
Size: 925 623
BsaBI GATnn'nnATC
Cuts at: 0 281 1548
Size: 281 1267
BseYI C'CCAG_C
Cuts at: 0 975 1548
Size: 975 573
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 676 1548
Size: 427 249 872
Fragments arranged by size:
872 427 249
BsiHKAI G_wGCw'C
Cuts at: 0 227 455 568 641 963 1375 1548
Size: 227 228 113 73 322 412 173
Fragments arranged by size:
412 322 228 227 173 113 73
BsmI GAATG_Cn'
Cuts at: 0 946 1548
Size: 946 602
Bsp1286I G_dGCh'C
Cuts at: 0 33 187 227 455 568 641 760 963
Size: 33 154 40 228 113 73 119 203
Cuts at: 963 1375 1548
Size: 412 173
Fragments arranged by size:
412 228 203 173 154 119 113 73
40 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 1548
Size: 410 1138
BsrDI GCAATG_nn'
Cuts at: 0 62 600 1548
Size: 62 538 948
Fragments arranged by size:
948 538 62
BstAPI GCAn_nnn'nTGC
Cuts at: 0 52 483 1548
Size: 52 431 1065
Fragments arranged by size:
1065 431 52
BstYI r'GATC_y
Cuts at: 0 884 901 1125 1548
Size: 884 17 224 423
Fragments arranged by size:
884 423 224 17
Bsu36I CC'TnA_GG
Cuts at: 0 202 1548
Size: 202 1346
BtgI C'CryG_G
Cuts at: 0 1062 1548
Size: 1062 486
BtsI GCAGTG_nn'
Cuts at: 0 1375 1548
Size: 1375 173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 976 1011 1548
Size: 976 35 537
Fragments arranged by size:
976 537 35
DraI TTT'AAA
Cuts at: 0 71 1548
Size: 71 1477
DraIII CAC_nnn'GTG
Cuts at: 0 162 1548
Size: 162 1386
EaeI y'GGCC_r
Cuts at: 0 19 1548
Size: 19 1529
EarI CTCTTCn'nnn_
Cuts at: 0 136 460 706 1548
Size: 136 324 246 842
Fragments arranged by size:
842 324 246 136
EcoICRI GAG'CTC
Cuts at: 0 225 453 1548
Size: 225 228 1095
Fragments arranged by size:
1095 228 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 568 1314 1548
Size: 234 334 746 234
Fragments arranged by size:
746 334 234 234
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 1477 1548
Size: 29 1 1447 71
Fragments arranged by size:
1447 71 29 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 1548
Size: 147 1401
HaeII r_GCGC'y
Cuts at: 0 950 1548
Size: 950 598
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 195 201 227 555 587 816 848
Size: 169 26 6 26 328 32 229 32
Cuts at: 848 1058 1090 1469 1501 1548
Size: 210 32 379 32 47
Fragments arranged by size:
379 328 229 210 169 47 32 32
32 32 26 26 6
HincII GTy'rAC
Cuts at: 0 256 1091 1548
Size: 256 835 457
Fragments arranged by size:
835 457 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 521 1153 1548
Size: 377 144 632 395
Fragments arranged by size:
632 395 377 144
MscI TGG'CCA
Cuts at: 0 21 1548
Size: 21 1527
MslI CAynn'nnrTG
Cuts at: 0 108 1105 1548
Size: 108 997 443
Fragments arranged by size:
997 443 108
MspA1I CmG'CkG
Cuts at: 0 116 432 648 1263 1524 1548
Size: 116 316 216 615 261 24
Fragments arranged by size:
615 316 261 216 116 24
NcoI C'CATG_G
Cuts at: 0 1062 1548
Size: 1062 486
NdeI CA'TA_TG
Cuts at: 0 1421 1548
Size: 1421 127
NspI r_CATG'y
Cuts at: 0 737 1434 1548
Size: 737 697 114
Fragments arranged by size:
737 697 114
PacI TTA_AT'TAA
Cuts at: 0 614 1548
Size: 614 934
PasI CC'CwG_GG
Cuts at: 0 87 1548
Size: 87 1461
PciI A'CATG_T
Cuts at: 0 733 1430 1548
Size: 733 697 118
Fragments arranged by size:
733 697 118
PfoI T'CCnGG_A
Cuts at: 0 1330 1548
Size: 1330 218
PpuMI rG'GwC_Cy
Cuts at: 0 1477 1548
Size: 1477 71
PspOMI G'GGCC_C
Cuts at: 0 29 1548
Size: 29 1519
PstI C_TGCA'G
Cuts at: 0 479 1203 1548
Size: 479 724 345
Fragments arranged by size:
724 479 345
PvuII CAG'CTG
Cuts at: 0 432 648 1524 1548
Size: 432 216 876 24
Fragments arranged by size:
876 432 216 24
SacI G_AGCT'C
Cuts at: 0 227 455 1548
Size: 227 228 1093
Fragments arranged by size:
1093 228 227
SapI GCTCTTCn'nnn_
Cuts at: 0 136 460 706 1548
Size: 136 324 246 842
Fragments arranged by size:
842 324 246 136
SfcI C'TryA_G
Cuts at: 0 475 1199 1548
Size: 475 724 349
Fragments arranged by size:
724 475 349
SmlI C'TyrA_G
Cuts at: 0 331 345 1548
Size: 331 14 1203
Fragments arranged by size:
1203 331 14
SspI AAT'ATT
Cuts at: 0 362 1548
Size: 362 1186
StyI C'CwwG_G
Cuts at: 0 987 1035 1062 1440 1548
Size: 987 48 27 378 108
Fragments arranged by size:
987 378 108 48 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 120 461 1048 1483 1548
Size: 120 341 587 435 65
Fragments arranged by size:
587 435 341 120 65
XbaI T'CTAG_A
Cuts at: 0 1050 1548
Size: 1050 498
XmnI GAAnn'nnTTC
Cuts at: 0 1229 1548
Size: 1229 319
Enzymes that do cut:
AcuI AflII AflIII AloI AlwNI ApaI ApoI AseI
BanII BglII BlpI Bme1580I BmrI BpmI BsaI BsaBI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstAPI
BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI NdeI NspI PacI
PasI PciI PfoI PpuMI PspOMI PstI PvuII SacI
SapI SfcI SmlI SspI StyI TaqII XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI ClaI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TatI TstI Tth111I
XcmI XhoI XmaI ZraI