Restriction map for
pF1KA0412
(Linear) (Six-base) MAPSORT of: KIAA0412.seq Check: 8628 from: 1 to: 1881
KIAA0412.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 402 1404 1626 1881
Size: 310 92 1002 222 255
Fragments arranged by size:
1002 310 255 222 92
AleI CACnn'nnGTG
Cuts at: 0 1013 1881
Size: 1013 868
ApaI G_GGCC'C
Cuts at: 0 30 1344 1881
Size: 30 1314 537
Fragments arranged by size:
1314 537 30
ApaLI G'TGCA_C
Cuts at: 0 948 1881
Size: 948 933
ApoI r'AATT_y
Cuts at: 0 668 1881
Size: 668 1213
AvaI C'yCGr_G
Cuts at: 0 1204 1881
Size: 1204 677
BanI G'GyrC_C
Cuts at: 0 1079 1247 1881
Size: 1079 168 634
Fragments arranged by size:
1079 634 168
BanII G_rGCy'C
Cuts at: 0 30 146 466 1344 1881
Size: 30 116 320 878 537
Fragments arranged by size:
878 537 320 116 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 1044 1128 1725 1865 1881
Size: 236 808 84 597 140 16
Fragments arranged by size:
808 597 236 140 84 16
BclI T'GATC_A
Cuts at: 0 1839 1881
Size: 1839 42
BfrBI ATG'CAT
Cuts at: 0 1680 1881
Size: 1680 201
BlpI GC'TnA_GC
Cuts at: 0 101 179 1881
Size: 101 78 1702
Fragments arranged by size:
1702 101 78
Bme1580I G_kGCm'C
Cuts at: 0 30 952 1344 1881
Size: 30 922 392 537
Fragments arranged by size:
922 537 392 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 770 1274 1694 1881
Size: 770 504 420 187
Fragments arranged by size:
770 504 420 187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 543 1881
Size: 336 207 1338
Fragments arranged by size:
1338 336 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 698 950 1202 1370 1454 1584 1622
Size: 515 183 252 252 168 84 130 38
Cuts at: 1622 1881
Size: 259
Fragments arranged by size:
515 259 252 252 183 168 130 84
38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 1881
Size: 1225 656
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 527 557 1881
Size: 527 30 1324
Fragments arranged by size:
1324 527 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1881
Size: 91 1790
BseYI C'CCAG_C
Cuts at: 0 824 1881
Size: 824 1057
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1137 1169 1305 1881
Size: 1137 32 136 576
Fragments arranged by size:
1137 576 136 32
BsiHKAI G_wGCw'C
Cuts at: 0 730 952 1570 1881
Size: 730 222 618 311
Fragments arranged by size:
730 618 311 222
BsmI GAATG_Cn'
Cuts at: 0 700 877 1881
Size: 700 177 1004
Fragments arranged by size:
1004 700 177
Bsp1286I G_dGCh'C
Cuts at: 0 30 146 466 730 952 1344 1570 1881
Size: 30 116 320 264 222 392 226 311
Fragments arranged by size:
392 320 311 264 226 222 116 30
BsrBI CCG'CTC
Cuts at: 0 896 1881
Size: 896 985
BsrDI GCAATG_nn'
Cuts at: 0 873 1881
Size: 873 1008
BssSI C'ACGA_G
Cuts at: 0 1494 1881
Size: 1494 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 872 1881
Size: 872 1009
BstYI r'GATC_y
Cuts at: 0 432 576 1881
Size: 432 144 1305
Fragments arranged by size:
1305 432 144
BtgI C'CryG_G
Cuts at: 0 214 1881
Size: 214 1667
BtsI GCAGTG_nn'
Cuts at: 0 621 1125 1293 1311 1881
Size: 621 504 168 18 570
Fragments arranged by size:
621 570 504 168 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1359 1394 1881
Size: 1359 35 487
Fragments arranged by size:
1359 487 35
DraI TTT'AAA
Cuts at: 0 609 1881
Size: 609 1272
EaeI y'GGCC_r
Cuts at: 0 960 1881
Size: 960 921
EarI CTCTTCn'nnn_
Cuts at: 0 587 1881
Size: 587 1294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 402 515 698 950 1202 1370 1404
Size: 310 92 113 183 252 252 168 34
Cuts at: 1404 1454 1584 1622 1626 1881
Size: 50 130 38 4 255
Fragments arranged by size:
310 255 252 252 183 168 130 113
92 50 38 34 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1881
Size: 115 1766
EcoO109I rG'GnC_Cy
Cuts at: 0 770 1340 1404 1881
Size: 770 570 64 477
Fragments arranged by size:
770 570 477 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1155 1881
Size: 1155 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 527 559 717 749 1223 1255
Size: 379 32 116 32 158 32 474 32
Cuts at: 1255 1807 1839 1881
Size: 552 32 42
Fragments arranged by size:
552 474 379 158 116 42 32 32
32 32 32
MscI TGG'CCA
Cuts at: 0 962 1881
Size: 962 919
MslI CAynn'nnrTG
Cuts at: 0 1013 1166 1480 1881
Size: 1013 153 314 401
Fragments arranged by size:
1013 401 314 153
MspA1I CmG'CkG
Cuts at: 0 10 90 105 490 834 1254 1881
Size: 10 80 15 385 344 420 627
Fragments arranged by size:
627 420 385 344 80 15 10
NcoI C'CATG_G
Cuts at: 0 214 1881
Size: 214 1667
NsiI A_TGCA'T
Cuts at: 0 1682 1881
Size: 1682 199
NspI r_CATG'y
Cuts at: 0 1485 1588 1881
Size: 1485 103 293
Fragments arranged by size:
1485 293 103
PfoI T'CCnGG_A
Cuts at: 0 531 1881
Size: 531 1350
PpuMI rG'GwC_Cy
Cuts at: 0 1404 1881
Size: 1404 477
PshAI GACnn'nnGTC
Cuts at: 0 248 1881
Size: 248 1633
PspOMI G'GGCC_C
Cuts at: 0 26 1340 1881
Size: 26 1314 541
Fragments arranged by size:
1314 541 26
PspXI vC'TCGA_Gb
Cuts at: 0 1204 1881
Size: 1204 677
PvuII CAG'CTG
Cuts at: 0 90 490 1881
Size: 90 400 1391
Fragments arranged by size:
1391 400 90
RsrII CG'GwC_CG
Cuts at: 0 21 1881
Size: 21 1860
SmlI C'TyrA_G
Cuts at: 0 1204 1240 1881
Size: 1204 36 641
Fragments arranged by size:
1204 641 36
SspI AAT'ATT
Cuts at: 0 1806 1881
Size: 1806 75
StuI AGG'CCT
Cuts at: 0 799 883 1303 1471 1639 1881
Size: 799 84 420 168 168 242
Fragments arranged by size:
799 420 242 168 168 84
StyI C'CwwG_G
Cuts at: 0 71 214 1881
Size: 71 143 1667
Fragments arranged by size:
1667 143 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 1324 1881
Size: 904 420 557
Fragments arranged by size:
904 557 420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 828 860 1500 1532 1881
Size: 338 32 458 32 640 32 349
Fragments arranged by size:
640 458 349 338 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 199 1881
Size: 199 1682
XhoI C'TCGA_G
Cuts at: 0 1204 1881
Size: 1204 677
XmnI GAAnn'nnTTC
Cuts at: 0 1135 1219 1276 1881
Size: 1135 84 57 605
Fragments arranged by size:
1135 605 84 57
Enzymes that do cut:
AcuI AleI ApaI ApaLI ApoI AvaI BanI BanII
BbsI BclI BfrBI BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrBI BsrDI BssSI BstAPI BstYI BtgI BtsI CspCI
DraI EaeI EarI Eco57MI EcoNI EcoO109I FalI Hin4I
MscI MslI MspA1I NcoI NsiI NspI PfoI PpuMI
PshAI PspOMI PspXI PvuII RsrII SmlI SspI StuI
StyI TaqII TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglI
BglII BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrFI
BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PsrI PstI PvuI SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TatI Tth111I XbaI XmaI ZraI