Restriction map for
pF1KSDA0410
(Linear) (Six-base) MAPSORT of: KIAA0410.seq Check: 7743 from: 1 to: 1455
KIAA0410.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 745 889 1020 1054 1192 1455
Size: 306 439 144 131 34 138 263
Fragments arranged by size:
439 306 263 144 138 131 34
AflII C'TTAA_G
Cuts at: 0 841 1455
Size: 841 614
AleI CACnn'nnGTG
Cuts at: 0 46 1455
Size: 46 1409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1442 1455
Size: 1442 13
AlwNI CAG_nnn'CTG
Cuts at: 0 352 879 1024 1455
Size: 352 527 145 431
Fragments arranged by size:
527 431 352 145
ApoI r'AATT_y
Cuts at: 0 778 1172 1455
Size: 778 394 283
Fragments arranged by size:
778 394 283
BanII G_rGCy'C
Cuts at: 0 40 125 200 1455
Size: 40 85 75 1255
Fragments arranged by size:
1255 85 75 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 818 994 1455
Size: 818 176 461
Fragments arranged by size:
818 461 176
BbvCI CC'TCA_GC
Cuts at: 0 387 1455
Size: 387 1068
BmrI ACTGGGnnnn_n'
Cuts at: 0 230 1455
Size: 230 1225
BmtI G_CTAG'C
Cuts at: 0 395 1455
Size: 395 1060
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 929 1455
Size: 929 526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 413 757 1455
Size: 140 273 344 698
Fragments arranged by size:
698 344 273 140
Bpu10I CC'TnA_GC
Cuts at: 0 387 522 947 1455
Size: 387 135 425 508
Fragments arranged by size:
508 425 387 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1130 1455
Size: 1103 27 325
Fragments arranged by size:
1103 325 27
BsaBI GATnn'nnATC
Cuts at: 0 734 1455
Size: 734 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 138 156 168 186 411 441 1268 1298
Size: 138 18 12 18 225 30 827 30
Cuts at: 1298 1455
Size: 157
Fragments arranged by size:
827 225 157 138 30 30 18 18
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 660 1455
Size: 660 795
BsmI GAATG_Cn'
Cuts at: 0 591 966 1444 1455
Size: 591 375 478 11
Fragments arranged by size:
591 478 375 11
Bsp1286I G_dGCh'C
Cuts at: 0 40 125 200 1455
Size: 40 85 75 1255
Fragments arranged by size:
1255 85 75 40
BspHI T'CATG_A
Cuts at: 0 1218 1455
Size: 1218 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 222 351 1455
Size: 222 129 1104
Fragments arranged by size:
1104 222 129
BsrDI GCAATG_nn'
Cuts at: 0 642 842 1455
Size: 642 200 613
Fragments arranged by size:
642 613 200
BsrFI r'CCGG_y
Cuts at: 0 55 1455
Size: 55 1400
BstAPI GCAn_nnn'nTGC
Cuts at: 0 352 1024 1390 1455
Size: 352 672 366 65
Fragments arranged by size:
672 366 352 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1455
Size: 48 1407
BstYI r'GATC_y
Cuts at: 0 205 1455
Size: 205 1250
BtgI C'CryG_G
Cuts at: 0 47 1394 1455
Size: 47 1347 61
Fragments arranged by size:
1347 61 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 345 380 1455
Size: 345 35 1075
Fragments arranged by size:
1075 345 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 408 1455
Size: 408 1047
EaeI y'GGCC_r
Cuts at: 0 50 1455
Size: 50 1405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 306 413 745 757 889 1020 1054
Size: 140 166 107 332 12 132 131 34
Cuts at: 1054 1192 1455
Size: 138 263
Fragments arranged by size:
332 263 166 140 138 132 131 107
34 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1430 1455
Size: 1430 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 856 1455
Size: 856 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 411 443 1455
Size: 411 32 1012
Fragments arranged by size:
1012 411 32
HincII GTy'rAC
Cuts at: 0 599 1455
Size: 599 856
MslI CAynn'nnrTG
Cuts at: 0 46 1013 1223 1455
Size: 46 967 210 232
Fragments arranged by size:
967 232 210 46
NaeI GCC'GGC
Cuts at: 0 57 1455
Size: 57 1398
NcoI C'CATG_G
Cuts at: 0 1394 1455
Size: 1394 61
NgoMIV G'CCGG_C
Cuts at: 0 55 1455
Size: 55 1400
NheI G'CTAG_C
Cuts at: 0 391 1455
Size: 391 1064
PflMI CCAn_nnn'nTGG
Cuts at: 0 1322 1455
Size: 1322 133
PfoI T'CCnGG_A
Cuts at: 0 25 1455
Size: 25 1430
PstI C_TGCA'G
Cuts at: 0 309 348 633 1455
Size: 309 39 285 822
Fragments arranged by size:
822 309 285 39
ScaI AGT'ACT
Cuts at: 0 147 1455
Size: 147 1308
SfcI C'TryA_G
Cuts at: 0 269 305 344 611 629 1257 1430 1455
Size: 269 36 39 267 18 628 173 25
Fragments arranged by size:
628 269 267 173 39 36 25 18
SgrAI Cr'CCGG_yG
Cuts at: 0 55 1455
Size: 55 1400
SmlI C'TyrA_G
Cuts at: 0 841 1118 1145 1455
Size: 841 277 27 310
Fragments arranged by size:
841 310 277 27
StuI AGG'CCT
Cuts at: 0 503 644 1455
Size: 503 141 811
Fragments arranged by size:
811 503 141
StyI C'CwwG_G
Cuts at: 0 645 1251 1394 1455
Size: 645 606 143 61
Fragments arranged by size:
645 606 143 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 1350 1455
Size: 454 896 105
Fragments arranged by size:
896 454 105
TatI w'GTAC_w
Cuts at: 0 145 553 1076 1455
Size: 145 408 523 379
Fragments arranged by size:
523 408 379 145
XmnI GAAnn'nnTTC
Cuts at: 0 147 825 1267 1444 1455
Size: 147 678 442 177 11
Fragments arranged by size:
678 442 177 147 11
Enzymes that do cut:
AcuI AflII AleI AlfI AlwNI ApoI BanII BbsI
BbvCI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI
BsaXI BseRI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BstXI BstYI BtgI CspCI DrdI EaeI Eco57MI
EcoNI FalI Hin4I HincII MslI NaeI NcoI NgoMIV
NheI PflMI PfoI PstI ScaI SfcI SgrAI SmlI
StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BsaI BsaAI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII EagI EarI
EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NarI NdeI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI