Restriction map for
pF1KA0408
(Linear) (Six-base) MAPSORT of: KIAA0408.seq Check: 2557 from: 1 to: 2841
KIAA0408.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1952 2841
Size: 1952 889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1594 1864 2134 2841
Size: 392 1202 270 270 707
Fragments arranged by size:
1202 707 392 270 270
AfeI AGC'GCT
Cuts at: 0 2203 2841
Size: 2203 638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1531 2173 2668 2841
Size: 1531 642 495 173
Fragments arranged by size:
1531 642 495 173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1652 1684 2841
Size: 1652 32 1157
Fragments arranged by size:
1652 1157 32
AlwNI CAG_nnn'CTG
Cuts at: 0 67 222 584 1944 2841
Size: 67 155 362 1360 897
Fragments arranged by size:
1360 897 362 155 67
ApaI G_GGCC'C
Cuts at: 0 952 2327 2411 2841
Size: 952 1375 84 430
Fragments arranged by size:
1375 952 430 84
AscI GG'CGCG_CC
Cuts at: 0 2122 2841
Size: 2122 719
AvaI C'yCGr_G
Cuts at: 0 427 440 469 2404 2674 2841
Size: 427 13 29 1935 270 167
Fragments arranged by size:
1935 427 270 167 29 13
BamHI G'GATC_C
Cuts at: 0 1220 2841
Size: 1220 1621
BanI G'GyrC_C
Cuts at: 0 324 2072 2400 2679 2704 2841
Size: 324 1748 328 279 25 137
Fragments arranged by size:
1748 328 324 279 137 25
BanII G_rGCy'C
Cuts at: 0 117 776 952 1670 1760 2030 2144 2327
Size: 117 659 176 718 90 270 114 183
Cuts at: 2327 2411 2717 2841
Size: 84 306 124
Fragments arranged by size:
718 659 306 270 183 176 124 117
114 90 84
BbeI G_GCGC'C
Cuts at: 0 328 2404 2708 2841
Size: 328 2076 304 133
Fragments arranged by size:
2076 328 304 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1964 2805 2841
Size: 1964 841 36
Fragments arranged by size:
1964 841 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1144 1178 1794 1828 2466 2500 2841
Size: 1144 34 616 34 638 34 341
Fragments arranged by size:
1144 638 616 341 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 70 310 2841
Size: 70 240 2531
Fragments arranged by size:
2531 240 70
BglII A'GATC_T
Cuts at: 0 1569 2841
Size: 1569 1272
BlpI GC'TnA_GC
Cuts at: 0 608 2143 2841
Size: 608 1535 698
Fragments arranged by size:
1535 698 608
Bme1580I G_kGCm'C
Cuts at: 0 461 952 2327 2411 2684 2841
Size: 461 491 1375 84 273 157
Fragments arranged by size:
1375 491 461 273 157 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 2841
Size: 172 2669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 657 2361 2841
Size: 657 1704 480
Fragments arranged by size:
1704 657 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1654 1843 2017 2841
Size: 1330 324 189 174 824
Fragments arranged by size:
1330 824 324 189 174
Bpu10I CC'TnA_GC
Cuts at: 0 1771 2841
Size: 1771 1070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1081 1306 1744 2841
Size: 775 306 225 438 1097
Fragments arranged by size:
1097 775 438 306 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 895 2841
Size: 895 1946
BsaAI yAC'GTr
Cuts at: 0 2538 2841
Size: 2538 303
BsaHI Gr'CG_yC
Cuts at: 0 316 325 2255 2401 2705 2841
Size: 316 9 1930 146 304 136
Fragments arranged by size:
1930 316 304 146 136 9
BsaWI w'CCGG_w
Cuts at: 0 700 2841
Size: 700 2141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1652 1682 2841
Size: 436 30 1186 30 1159
Fragments arranged by size:
1186 1159 436 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 302 445 515 533 536 648 1075 1171
Size: 302 143 70 18 3 112 427 96
Cuts at: 1171 1570 2014 2122 2365 2587 2725 2841
Size: 399 444 108 243 222 138 116
Fragments arranged by size:
444 427 399 302 243 222 143 138
116 112 108 96 70 18 3
BseYI C'CCAG_C
Cuts at: 0 953 1738 2841
Size: 953 785 1103
Fragments arranged by size:
1103 953 785
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 2841
Size: 64 2777
BsiEI CG_ry'CG
Cuts at: 0 821 2433 2490 2670 2700 2841
Size: 821 1612 57 180 30 141
Fragments arranged by size:
1612 821 180 141 57 30
BsiHKAI G_wGCw'C
Cuts at: 0 117 776 1670 1760 2030 2144 2512 2603
Size: 117 659 894 90 270 114 368 91
Cuts at: 2603 2841
Size: 238
Fragments arranged by size:
894 659 368 270 238 117 114 91
90
BsiWI C'GTAC_G
Cuts at: 0 600 2841
Size: 600 2241
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1957 2841
Size: 1957 884
Bsp1286I G_dGCh'C
Cuts at: 0 117 461 776 952 1670 1760 2030 2144
Size: 117 344 315 176 718 90 270 114
Cuts at: 2144 2327 2411 2512 2603 2684 2717 2841
Size: 183 84 101 91 81 33 124
Fragments arranged by size:
718 344 315 270 183 176 124 117
114 101 91 90 84 81 33
BspEI T'CCGG_A
Cuts at: 0 700 2841
Size: 700 2141
BspHI T'CATG_A
Cuts at: 0 1901 2841
Size: 1901 940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1952 2841
Size: 1952 889
BsrBI CCG'CTC
Cuts at: 0 340 486 801 980 1899 2456 2841
Size: 340 146 315 179 919 557 385
Fragments arranged by size:
919 557 385 340 315 179 146
BsrFI r'CCGG_y
Cuts at: 0 187 526 685 2489 2841
Size: 187 339 159 1804 352
Fragments arranged by size:
1804 352 339 187 159
BssHII G'CGCG_C
Cuts at: 0 2066 2122 2527 2841
Size: 2066 56 405 314
Fragments arranged by size:
2066 405 314 56
BssSI C'ACGA_G
Cuts at: 0 1189 1663 2505 2602 2841
Size: 1189 474 842 97 239
Fragments arranged by size:
1189 842 474 239 97
BstAPI GCAn_nnn'nTGC
Cuts at: 0 222 1182 1944 2607 2841
Size: 222 960 762 663 234
Fragments arranged by size:
960 762 663 234 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1800 2841
Size: 1800 1041
BstYI r'GATC_y
Cuts at: 0 1220 1569 1681 1695 2841
Size: 1220 349 112 14 1146
Fragments arranged by size:
1220 1146 349 112 14
Bsu36I CC'TnA_GG
Cuts at: 0 1864 2841
Size: 1864 977
BtgI C'CryG_G
Cuts at: 0 821 2232 2700 2841
Size: 821 1411 468 141
Fragments arranged by size:
1411 821 468 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1886 2481 2841
Size: 1886 595 360
Fragments arranged by size:
1886 595 360
ClaI AT'CG_AT
Cuts at: 0 1109 2841
Size: 1109 1732
DraI TTT'AAA
Cuts at: 0 2805 2841
Size: 2805 36
EaeI y'GGCC_r
Cuts at: 0 164 395 818 908 1628 1889 1988 2055
Size: 164 231 423 90 720 261 99 67
Cuts at: 2055 2540 2841
Size: 485 301
Fragments arranged by size:
720 485 423 301 261 231 164 99
90 67
EagI C'GGCC_G
Cuts at: 0 818 2841
Size: 818 2023
EarI CTCTTCn'nnn_
Cuts at: 0 666 1494 2841
Size: 666 828 1347
Fragments arranged by size:
1347 828 666
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 308 909 958 1864 2038 2841
Size: 308 601 49 906 174 803
Fragments arranged by size:
906 803 601 308 174 49
EcoICRI GAG'CTC
Cuts at: 0 115 774 1668 1758 2028 2142 2841
Size: 115 659 894 90 270 114 699
Fragments arranged by size:
894 699 659 270 115 114 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1330 1594 1654 1843 1864 2017 2134
Size: 392 938 264 60 189 21 153 117
Cuts at: 2134 2841
Size: 707
Fragments arranged by size:
938 707 392 264 189 153 117 60
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1312 2104 2841
Size: 1312 792 737
Fragments arranged by size:
1312 792 737
EcoO109I rG'GnC_Cy
Cuts at: 0 638 948 949 1317 1732 2097 2323 2408
Size: 638 310 1 368 415 365 226 85
Cuts at: 2408 2550 2841
Size: 142 291
Fragments arranged by size:
638 415 368 365 310 291 226 142
85 1
FspI TGC'GCA
Cuts at: 0 1303 2359 2841
Size: 1303 1056 482
Fragments arranged by size:
1303 1056 482
HaeII r_GCGC'y
Cuts at: 0 26 328 560 1006 2205 2404 2472 2708
Size: 26 302 232 446 1199 199 68 236
Cuts at: 2708 2841
Size: 133
Fragments arranged by size:
1199 446 302 236 232 199 133 68
26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 436 468 2200 2232 2278 2310
Size: 143 32 261 32 1732 32 46 32
Cuts at: 2310 2440 2472 2841
Size: 130 32 369
Fragments arranged by size:
1732 369 261 143 130 46 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1171 2841
Size: 1171 1670
HindIII A'AGCT_T
Cuts at: 0 1451 2841
Size: 1451 1390
KasI G'GCGC_C
Cuts at: 0 324 2400 2704 2841
Size: 324 2076 304 137
Fragments arranged by size:
2076 324 304 137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1907 2216 2841
Size: 1175 732 309 625
Fragments arranged by size:
1175 732 625 309
MscI TGG'CCA
Cuts at: 0 1630 2542 2841
Size: 1630 912 299
Fragments arranged by size:
1630 912 299
MslI CAynn'nnrTG
Cuts at: 0 2536 2722 2841
Size: 2536 186 119
Fragments arranged by size:
2536 186 119
MspA1I CmG'CkG
Cuts at: 0 58 222 626 728 811 823 1584 1950
Size: 58 164 404 102 83 12 761 366
Cuts at: 1950 2147 2163 2234 2369 2702 2841
Size: 197 16 71 135 333 139
Fragments arranged by size:
761 404 366 333 197 164 139 135
102 83 71 58 16 12
NaeI GCC'GGC
Cuts at: 0 189 528 2841
Size: 189 339 2313
Fragments arranged by size:
2313 339 189
NarI GG'CG_CC
Cuts at: 0 325 2401 2705 2841
Size: 325 2076 304 136
Fragments arranged by size:
2076 325 304 136
NgoMIV G'CCGG_C
Cuts at: 0 187 526 2841
Size: 187 339 2315
Fragments arranged by size:
2315 339 187
NotI GC'GGCC_GC
Cuts at: 0 818 2841
Size: 818 2023
NspI r_CATG'y
Cuts at: 0 993 2199 2841
Size: 993 1206 642
Fragments arranged by size:
1206 993 642
PasI CC'CwG_GG
Cuts at: 0 578 2841
Size: 578 2263
PflMI CCAn_nnn'nTGG
Cuts at: 0 360 584 1194 2841
Size: 360 224 610 1647
Fragments arranged by size:
1647 610 360 224
PfoI T'CCnGG_A
Cuts at: 0 342 2594 2841
Size: 342 2252 247
Fragments arranged by size:
2252 342 247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 1652 1684 2841
Size: 126 32 1494 32 1157
Fragments arranged by size:
1494 1157 126 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1317 2550 2841
Size: 1317 1233 291
Fragments arranged by size:
1317 1233 291
PspOMI G'GGCC_C
Cuts at: 0 948 2323 2407 2841
Size: 948 1375 84 434
Fragments arranged by size:
1375 948 434 84
PspXI vC'TCGA_Gb
Cuts at: 0 2674 2841
Size: 2674 167
PstI C_TGCA'G
Cuts at: 0 63 227 767 1178 1229 1589 1949 2105
Size: 63 164 540 411 51 360 360 156
Cuts at: 2105 2132 2168 2657 2841
Size: 27 36 489 184
Fragments arranged by size:
540 489 411 360 360 184 164 156
63 51 36 27
PvuI CG_AT'CG
Cuts at: 0 2433 2841
Size: 2433 408
PvuII CAG'CTG
Cuts at: 0 222 1584 1950 2163 2841
Size: 222 1362 366 213 678
Fragments arranged by size:
1362 678 366 222 213
RsrII CG'GwC_CG
Cuts at: 0 421 2841
Size: 421 2420
SacI G_AGCT'C
Cuts at: 0 117 776 1670 1760 2030 2144 2841
Size: 117 659 894 90 270 114 697
Fragments arranged by size:
894 697 659 270 117 114 90
SacII CC_GC'GG
Cuts at: 0 824 2235 2703 2841
Size: 824 1411 468 138
Fragments arranged by size:
1411 824 468 138
SbfI CC_TGCA'GG
Cuts at: 0 2105 2841
Size: 2105 736
SfcI C'TryA_G
Cuts at: 0 59 223 763 1174 1225 1585 1945 2101
Size: 59 164 540 411 51 360 360 156
Cuts at: 2101 2128 2164 2653 2841
Size: 27 36 489 188
Fragments arranged by size:
540 489 411 360 360 188 164 156
59 51 36 27
SfoI GGC'GCC
Cuts at: 0 326 2402 2706 2841
Size: 326 2076 304 135
Fragments arranged by size:
2076 326 304 135
SmaI CCC'GGG
Cuts at: 0 2406 2841
Size: 2406 435
SmlI C'TyrA_G
Cuts at: 0 790 1096 1321 1759 2674 2841
Size: 790 306 225 438 915 167
Fragments arranged by size:
915 790 438 306 225 167
SphI G_CATG'C
Cuts at: 0 993 2841
Size: 993 1848
SrfI GCCC'GGGC
Cuts at: 0 2406 2841
Size: 2406 435
SspI AAT'ATT
Cuts at: 0 2801 2841
Size: 2801 40
StuI AGG'CCT
Cuts at: 0 1841 2638 2841
Size: 1841 797 203
Fragments arranged by size:
1841 797 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 147 2107 2841
Size: 147 1960 734
Fragments arranged by size:
1960 734 147
TatI w'GTAC_w
Cuts at: 0 1676 2036 2042 2835 2841
Size: 1676 360 6 793 6
Fragments arranged by size:
1676 793 360 6 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 361 2841
Size: 361 2480
XhoI C'TCGA_G
Cuts at: 0 2674 2841
Size: 2674 167
XmaI C'CCGG_G
Cuts at: 0 2404 2841
Size: 2404 437
XmnI GAAnn'nnTTC
Cuts at: 0 2638 2841
Size: 2638 203
Enzymes that do cut:
AarI AcuI AfeI AlfI AloI AlwNI ApaI AscI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI ClaI DraI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SbfI SfcI SfoI SmaI SmlI SphI SrfI SspI
StuI TaqII TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI ApaLI ApoI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BmgBI BmtI BsaBI BsmI BsrDI
BsrGI BstBI BstEII BstZ17I BtsI CspCI DraIII DrdI
EcoRI EcoRV FalI FseI FspAI HpaI KpnI MfeI
MluI NcoI NdeI NheI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PsrI SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI StyI
SwaI TstI Tth111I XbaI ZraI