Restriction map for
pF1KA0406
(Linear) (Six-base) MAPSORT of: KIAA0406.seq Check: 6941 from: 1 to: 3267
KIAA0406.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1810 3267
Size: 1810 1457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 127 199 1018 2438 2668 2671 3267
Size: 118 9 72 819 1420 230 3 596
Fragments arranged by size:
1420 819 596 230 118 72 9 3
AflIII A'CryG_T
Cuts at: 0 253 3267
Size: 253 3014
AhdI GACnn_n'nnGTC
Cuts at: 0 188 1119 3267
Size: 188 931 2148
Fragments arranged by size:
2148 931 188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 1157 3267
Size: 406 751 2110
Fragments arranged by size:
2110 751 406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2498 2530 2594 2626 3267
Size: 2498 32 64 32 641
Fragments arranged by size:
2498 641 64 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1597 2067 2967 3267
Size: 1597 470 900 300
Fragments arranged by size:
1597 900 470 300
ApaI G_GGCC'C
Cuts at: 0 1237 2974 3267
Size: 1237 1737 293
Fragments arranged by size:
1737 1237 293
ApoI r'AATT_y
Cuts at: 0 1189 2079 3267
Size: 1189 890 1188
Fragments arranged by size:
1189 1188 890
AseI AT'TA_AT
Cuts at: 0 388 3267
Size: 388 2879
AvrII C'CTAG_G
Cuts at: 0 2994 3267
Size: 2994 273
BanI G'GyrC_C
Cuts at: 0 2333 3267
Size: 2333 934
BanII G_rGCy'C
Cuts at: 0 434 1237 2974 3267
Size: 434 803 1737 293
Fragments arranged by size:
1737 803 434 293
BbsI GAAGACnn'nnnn_
Cuts at: 0 187 2426 3081 3267
Size: 187 2239 655 186
Fragments arranged by size:
2239 655 187 186
BbvCI CC'TCA_GC
Cuts at: 0 1386 3267
Size: 1386 1881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3222 3256 3267
Size: 3222 34 11
Fragments arranged by size:
3222 34 11
BclI T'GATC_A
Cuts at: 0 2069 3267
Size: 2069 1198
BfrBI ATG'CAT
Cuts at: 0 1883 3267
Size: 1883 1384
BglI GCCn_nnn'nGGC
Cuts at: 0 2770 3267
Size: 2770 497
BglII A'GATC_T
Cuts at: 0 710 3267
Size: 710 2557
Bme1580I G_kGCm'C
Cuts at: 0 132 1237 2336 2974 3267
Size: 132 1105 1099 638 293
Fragments arranged by size:
1105 1099 638 293 132
BmgBI CAC'GTC
Cuts at: 0 1317 1787 2595 3267
Size: 1317 470 808 672
Fragments arranged by size:
1317 808 672 470
BmrI ACTGGGnnnn_n'
Cuts at: 0 1041 2298 3267
Size: 1041 1257 969
Fragments arranged by size:
1257 1041 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1258 1627 1990 2000 3229 3267
Size: 262 996 369 363 10 1229 38
Fragments arranged by size:
1229 996 369 363 262 38 10
Bpu10I CC'TnA_GC
Cuts at: 0 1386 3267
Size: 1386 1881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1482 2416 3238 3267
Size: 1482 934 822 29
Fragments arranged by size:
1482 934 822 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 1976 3267
Size: 1976 1291
BsaAI yAC'GTr
Cuts at: 0 2800 3267
Size: 2800 467
BsaBI GATnn'nnATC
Cuts at: 0 2630 3267
Size: 2630 637
BsaHI Gr'CG_yC
Cuts at: 0 2762 3267
Size: 2762 505
BsaWI w'CCGG_w
Cuts at: 0 1553 3267
Size: 1553 1714
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3103 3133 3267
Size: 3103 30 134
Fragments arranged by size:
3103 134 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 364 1431 1762 3267
Size: 37 327 1067 331 1505
Fragments arranged by size:
1505 1067 331 327 37
BseYI C'CCAG_C
Cuts at: 0 319 603 1600 3178 3267
Size: 319 284 997 1578 89
Fragments arranged by size:
1578 997 319 284 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 981 3178 3184 3214 3267
Size: 629 352 2197 6 30 53
Fragments arranged by size:
2197 629 352 53 30 6
BsiHKAI G_wGCw'C
Cuts at: 0 797 1766 2407 3245 3267
Size: 797 969 641 838 22
Fragments arranged by size:
969 838 797 641 22
BsmI GAATG_Cn'
Cuts at: 0 232 1134 1374 1883 3267
Size: 232 902 240 509 1384
Fragments arranged by size:
1384 902 509 240 232
Bsp1286I G_dGCh'C
Cuts at: 0 132 434 797 1237 1766 2336 2407 2974
Size: 132 302 363 440 529 570 71 567
Cuts at: 2974 3245 3267
Size: 271 22
Fragments arranged by size:
570 567 529 440 363 302 271 132
71 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 342 792 1458 1810 2195 3267
Size: 342 450 666 352 385 1072
Fragments arranged by size:
1072 666 450 385 352 342
BsrDI GCAATG_nn'
Cuts at: 0 2569 3267
Size: 2569 698
BsrFI r'CCGG_y
Cuts at: 0 2837 3267
Size: 2837 430
BstAPI GCAn_nnn'nTGC
Cuts at: 0 784 3267
Size: 784 2483
BstEII G'GTnAC_C
Cuts at: 0 1716 3004 3267
Size: 1716 1288 263
Fragments arranged by size:
1716 1288 263
BstYI r'GATC_y
Cuts at: 0 710 1617 2102 2576 2667 3267
Size: 710 907 485 474 91 600
Fragments arranged by size:
907 710 600 485 474 91
Bsu36I CC'TnA_GG
Cuts at: 0 20 3267
Size: 20 3247
BtgI C'CryG_G
Cuts at: 0 2588 3267
Size: 2588 679
BtsI GCAGTG_nn'
Cuts at: 0 556 2563 2780 3267
Size: 556 2007 217 487
Fragments arranged by size:
2007 556 487 217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2552 2587 3267
Size: 2552 35 680
Fragments arranged by size:
2552 680 35
DraI TTT'AAA
Cuts at: 0 675 2111 3267
Size: 675 1436 1156
Fragments arranged by size:
1436 1156 675
DraIII CAC_nnn'GTG
Cuts at: 0 3192 3267
Size: 3192 75
EaeI y'GGCC_r
Cuts at: 0 1902 2225 3267
Size: 1902 323 1042
Fragments arranged by size:
1902 1042 323
EarI CTCTTCn'nnn_
Cuts at: 0 163 1157 2354 2395 2495 3267
Size: 163 994 1197 41 100 772
Fragments arranged by size:
1197 994 772 163 100 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2632 3267
Size: 2632 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 127 199 262 1018 1258 1627 1990
Size: 118 9 72 63 756 240 369 363
Cuts at: 1990 2000 2438 2668 2671 3229 3267
Size: 10 438 230 3 558 38
Fragments arranged by size:
756 558 438 369 363 240 230 118
72 63 38 10 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 2998 3267
Size: 997 2001 269
Fragments arranged by size:
2001 997 269
EcoO109I rG'GnC_Cy
Cuts at: 0 190 945 2151 2889 2964 2971 3267
Size: 190 755 1206 738 75 7 296
Fragments arranged by size:
1206 755 738 296 190 75 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 1105 1137 2494 2526 3103 3135
Size: 174 32 899 32 1357 32 577 32
Cuts at: 3135 3267
Size: 132
Fragments arranged by size:
1357 899 577 174 132 32 32 32
32
HincII GTy'rAC
Cuts at: 0 860 3267
Size: 860 2407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1871 3267
Size: 1330 541 1396
Fragments arranged by size:
1396 1330 541
MscI TGG'CCA
Cuts at: 0 1904 2227 3267
Size: 1904 323 1040
Fragments arranged by size:
1904 1040 323
MslI CAynn'nnrTG
Cuts at: 0 388 2023 2593 3267
Size: 388 1635 570 674
Fragments arranged by size:
1635 674 570 388
MspA1I CmG'CkG
Cuts at: 0 603 1278 2416 2706 2949 3198 3212 3249
Size: 603 675 1138 290 243 249 14 37
Cuts at: 3249 3267
Size: 18
Fragments arranged by size:
1138 675 603 290 249 243 37 18
14
NcoI C'CATG_G
Cuts at: 0 2588 3267
Size: 2588 679
NdeI CA'TA_TG
Cuts at: 0 1914 3267
Size: 1914 1353
NsiI A_TGCA'T
Cuts at: 0 1885 3267
Size: 1885 1382
NspI r_CATG'y
Cuts at: 0 257 2284 3267
Size: 257 2027 983
Fragments arranged by size:
2027 983 257
PasI CC'CwG_GG
Cuts at: 0 2805 3267
Size: 2805 462
PciI A'CATG_T
Cuts at: 0 253 3267
Size: 253 3014
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 219 3267
Size: 78 141 3048
Fragments arranged by size:
3048 141 78
PfoI T'CCnGG_A
Cuts at: 0 278 2153 3267
Size: 278 1875 1114
Fragments arranged by size:
1875 1114 278
PpuMI rG'GwC_Cy
Cuts at: 0 190 945 2151 3267
Size: 190 755 1206 1116
Fragments arranged by size:
1206 1116 755 190
PshAI GACnn'nnGTC
Cuts at: 0 2543 3267
Size: 2543 724
PspOMI G'GGCC_C
Cuts at: 0 1233 2970 3267
Size: 1233 1737 297
Fragments arranged by size:
1737 1233 297
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3130 3162 3267
Size: 3130 32 105
Fragments arranged by size:
3130 105 32
PstI C_TGCA'G
Cuts at: 0 101 789 2063 3266 3267
Size: 101 688 1274 1203 1
Fragments arranged by size:
1274 1203 688 101 1
PvuII CAG'CTG
Cuts at: 0 603 2416 2706 2949 3198 3249 3267
Size: 603 1813 290 243 249 51 18
Fragments arranged by size:
1813 603 290 249 243 51 18
SapI GCTCTTCn'nnn_
Cuts at: 0 2395 3267
Size: 2395 872
ScaI AGT'ACT
Cuts at: 0 1967 3267
Size: 1967 1300
SexAI A'CCwGG_T
Cuts at: 0 3124 3267
Size: 3124 143
SfcI C'TryA_G
Cuts at: 0 97 785 2059 3262 3267
Size: 97 688 1274 1203 5
Fragments arranged by size:
1274 1203 688 97 5
SmlI C'TyrA_G
Cuts at: 0 1461 2395 3253 3267
Size: 1461 934 858 14
Fragments arranged by size:
1461 934 858 14
SnaBI TAC'GTA
Cuts at: 0 2800 3267
Size: 2800 467
SpeI A'CTAG_T
Cuts at: 0 1014 3267
Size: 1014 2253
SphI G_CATG'C
Cuts at: 0 2284 3267
Size: 2284 983
StuI AGG'CCT
Cuts at: 0 28 479 1003 1772 2728 3267
Size: 28 451 524 769 956 539
Fragments arranged by size:
956 769 539 524 451 28
StyI C'CwwG_G
Cuts at: 0 571 1399 2588 2714 2994 3267
Size: 571 828 1189 126 280 273
Fragments arranged by size:
1189 828 571 280 273 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 917 1781 2899 3267
Size: 333 584 864 1118 368
Fragments arranged by size:
1118 864 584 368 333
TatI w'GTAC_w
Cuts at: 0 149 1965 3267
Size: 149 1816 1302
Fragments arranged by size:
1816 1302 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 3267
Size: 1137 32 2098
Fragments arranged by size:
2098 1137 32
XbaI T'CTAG_A
Cuts at: 0 1305 3267
Size: 1305 1962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2022 2595 3122 3267
Size: 2022 573 527 145
Fragments arranged by size:
2022 573 527 145
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AloI AlwNI ApaI
ApoI AseI AvrII BanI BanII BbsI BbvCI BcgI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI
PspOMI PsrI PstI PvuII SapI ScaI SexAI SfcI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
ApaLI AscI AsiSI AvaI BaeI BamHI BbeI BciVI
BlpI BmtI BplI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI
ClaI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PmeI PmlI PpiI PsiI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SrfI SspI SwaI Tth111I XhoI XmaI XmnI
ZraI