Restriction map for
pF1KSDA0398
(Linear) (Six-base) MAPSORT of: KIAA0398.seq Check: 1710 from: 1 to: 1428
KIAA0398.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 158 1169 1428
Size: 158 1011 259
Fragments arranged by size:
1011 259 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 451 974 1428
Size: 314 137 523 454
Fragments arranged by size:
523 454 314 137
AflII C'TTAA_G
Cuts at: 0 1365 1428
Size: 1365 63
AleI CACnn'nnGTG
Cuts at: 0 825 1428
Size: 825 603
AlwNI CAG_nnn'CTG
Cuts at: 0 115 448 1428
Size: 115 333 980
Fragments arranged by size:
980 333 115
ApoI r'AATT_y
Cuts at: 0 6 66 556 766 808 1030 1108 1189
Size: 6 60 490 210 42 222 78 81
Cuts at: 1189 1428
Size: 239
Fragments arranged by size:
490 239 222 210 81 78 60 42
6
AseI AT'TA_AT
Cuts at: 0 95 1428
Size: 95 1333
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 1428
Size: 74 1354
BglI GCCn_nnn'nGGC
Cuts at: 0 932 1428
Size: 932 496
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 1428
Size: 131 1297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1428
Size: 377 1051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1428
Size: 412 1016
BsaHI Gr'CG_yC
Cuts at: 0 978 1428
Size: 978 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1428
Size: 782 646
BsmI GAATG_Cn'
Cuts at: 0 1247 1428
Size: 1247 181
BsrDI GCAATG_nn'
Cuts at: 0 1126 1428
Size: 1126 302
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 1428
Size: 10 1418
BstEII G'GTnAC_C
Cuts at: 0 1349 1428
Size: 1349 79
EarI CTCTTCn'nnn_
Cuts at: 0 235 1428
Size: 235 1193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 377 451 974 1428
Size: 314 63 74 523 454
Fragments arranged by size:
523 454 314 74 63
EcoRI G'AATT_C
Cuts at: 0 66 1108 1189 1428
Size: 66 1042 81 239
Fragments arranged by size:
1042 239 81 66
EcoRV GAT'ATC
Cuts at: 0 594 1428
Size: 594 834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 211 1370 1428
Size: 211 1159 58
Fragments arranged by size:
1159 211 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1428
Size: 217 32 1179
Fragments arranged by size:
1179 217 32
HindIII A'AGCT_T
Cuts at: 0 986 1428
Size: 986 442
MslI CAynn'nnrTG
Cuts at: 0 458 825 1428
Size: 458 367 603
Fragments arranged by size:
603 458 367
MspA1I CmG'CkG
Cuts at: 0 714 1428
Size: 714 714
NspI r_CATG'y
Cuts at: 0 1318 1428
Size: 1318 110
PasI CC'CwG_GG
Cuts at: 0 928 1428
Size: 928 500
PshAI GACnn'nnGTC
Cuts at: 0 142 1428
Size: 142 1286
PsiI TTA'TAA
Cuts at: 0 772 1428
Size: 772 656
PstI C_TGCA'G
Cuts at: 0 844 1428
Size: 844 584
SfcI C'TryA_G
Cuts at: 0 147 840 1428
Size: 147 693 588
Fragments arranged by size:
693 588 147
SmlI C'TyrA_G
Cuts at: 0 391 1365 1428
Size: 391 974 63
Fragments arranged by size:
974 391 63
SpeI A'CTAG_T
Cuts at: 0 1164 1428
Size: 1164 264
SspI AAT'ATT
Cuts at: 0 93 1428
Size: 93 1335
StuI AGG'CCT
Cuts at: 0 1249 1428
Size: 1249 179
StyI C'CwwG_G
Cuts at: 0 1250 1428
Size: 1250 178
TatI w'GTAC_w
Cuts at: 0 673 1151 1325 1428
Size: 673 478 174 103
Fragments arranged by size:
673 478 174 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 525 557 1428
Size: 525 32 871
Fragments arranged by size:
871 525 32
Enzymes that do cut:
AccI AcuI AflII AleI AlwNI ApoI AseI BbsI
BglI BmrI BpmI BpuEI BsaHI BsgI BsmI BsrDI
BstAPI BstEII EarI Eco57MI EcoRI EcoRV FalI Hin4I
HindIII MslI MspA1I NspI PasI PshAI PsiI PstI
SfcI SmlI SpeI SspI StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI