Restriction map for
pF1KA0391
(Linear) (Six-base) MAPSORT of: KIAA0391.seq Check: 8188 from: 1 to: 1701
KIAA0391.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 596 1701
Size: 320 276 1105
Fragments arranged by size:
1105 320 276
AflII C'TTAA_G
Cuts at: 0 379 1054 1123 1701
Size: 379 675 69 578
Fragments arranged by size:
675 578 379 69
AhdI GACnn_n'nnGTC
Cuts at: 0 1273 1701
Size: 1273 428
AleI CACnn'nnGTG
Cuts at: 0 1594 1701
Size: 1594 107
ApoI r'AATT_y
Cuts at: 0 288 357 792 1701
Size: 288 69 435 909
Fragments arranged by size:
909 435 288 69
AvrII C'CTAG_G
Cuts at: 0 1278 1701
Size: 1278 423
BanI G'GyrC_C
Cuts at: 0 315 1701
Size: 315 1386
BanII G_rGCy'C
Cuts at: 0 52 762 1701
Size: 52 710 939
Fragments arranged by size:
939 710 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 634 1643 1701
Size: 634 1009 58
Fragments arranged by size:
1009 634 58
BciVI GTATCCnnnnn_n'
Cuts at: 0 760 950 1701
Size: 760 190 751
Fragments arranged by size:
760 751 190
Bme1580I G_kGCm'C
Cuts at: 0 77 1701
Size: 77 1624
BmrI ACTGGGnnnn_n'
Cuts at: 0 1305 1701
Size: 1305 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1628 1701
Size: 281 1347 73
Fragments arranged by size:
1347 281 73
Bpu10I CC'TnA_GC
Cuts at: 0 269 1701
Size: 269 1432
BsaI GGTCTCn'nnnn_
Cuts at: 0 1186 1439 1701
Size: 1186 253 262
Fragments arranged by size:
1186 262 253
BsaWI w'CCGG_w
Cuts at: 0 403 1701
Size: 403 1298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 138 168 1701
Size: 138 30 1533
Fragments arranged by size:
1533 138 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 1701
Size: 379 1322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1701
Size: 1396 305
BsiHKAI G_wGCw'C
Cuts at: 0 762 1701
Size: 762 939
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1245 1701
Size: 1245 456
Bsp1286I G_dGCh'C
Cuts at: 0 52 77 762 1701
Size: 52 25 685 939
Fragments arranged by size:
939 685 52 25
BspHI T'CATG_A
Cuts at: 0 816 1067 1701
Size: 816 251 634
Fragments arranged by size:
816 634 251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1421 1701
Size: 1421 280
BssSI C'ACGA_G
Cuts at: 0 1614 1701
Size: 1614 87
BstBI TT'CG_AA
Cuts at: 0 24 419 1701
Size: 24 395 1282
Fragments arranged by size:
1282 395 24
BstEII G'GTnAC_C
Cuts at: 0 1090 1701
Size: 1090 611
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1676 1701
Size: 1676 25
Bsu36I CC'TnA_GG
Cuts at: 0 1115 1701
Size: 1115 586
BtsI GCAGTG_nn'
Cuts at: 0 1408 1425 1701
Size: 1408 17 276
Fragments arranged by size:
1408 276 17
DraI TTT'AAA
Cuts at: 0 392 845 1701
Size: 392 453 856
Fragments arranged by size:
856 453 392
EaeI y'GGCC_r
Cuts at: 0 987 1701
Size: 987 714
EarI CTCTTCn'nnn_
Cuts at: 0 40 1328 1701
Size: 40 1288 373
Fragments arranged by size:
1288 373 40
EcoICRI GAG'CTC
Cuts at: 0 760 1701
Size: 760 941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 320 596 1628 1701
Size: 281 39 276 1032 73
Fragments arranged by size:
1032 281 276 73 39
EcoRI G'AATT_C
Cuts at: 0 288 357 1701
Size: 288 69 1344
Fragments arranged by size:
1344 288 69
EcoRV GAT'ATC
Cuts at: 0 199 1701
Size: 199 1502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 1098 1130 1259 1291 1360 1392
Size: 276 32 790 32 129 32 69 32
Cuts at: 1392 1701
Size: 309
Fragments arranged by size:
790 309 276 129 69 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 27 37 848 1701
Size: 27 10 811 853
Fragments arranged by size:
853 811 27 10
MfeI C'AATT_G
Cuts at: 0 1532 1701
Size: 1532 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 815 1701
Size: 406 409 886
Fragments arranged by size:
886 409 406
MscI TGG'CCA
Cuts at: 0 989 1701
Size: 989 712
MslI CAynn'nnrTG
Cuts at: 0 80 773 1594 1701
Size: 80 693 821 107
Fragments arranged by size:
821 693 107 80
MspA1I CmG'CkG
Cuts at: 0 107 936 1029 1355 1527 1701
Size: 107 829 93 326 172 174
Fragments arranged by size:
829 326 174 172 107 93
NdeI CA'TA_TG
Cuts at: 0 563 1629 1701
Size: 563 1066 72
Fragments arranged by size:
1066 563 72
NspI r_CATG'y
Cuts at: 0 1296 1701
Size: 1296 405
PasI CC'CwG_GG
Cuts at: 0 70 1701
Size: 70 1631
PflMI CCAn_nnn'nTGG
Cuts at: 0 58 1701
Size: 58 1643
PfoI T'CCnGG_A
Cuts at: 0 1417 1548 1701
Size: 1417 131 153
Fragments arranged by size:
1417 153 131
PsiI TTA'TAA
Cuts at: 0 718 1701
Size: 718 983
PstI C_TGCA'G
Cuts at: 0 1432 1701
Size: 1432 269
PvuII CAG'CTG
Cuts at: 0 936 1029 1355 1527 1701
Size: 936 93 326 172 174
Fragments arranged by size:
936 326 174 172 93
SacI G_AGCT'C
Cuts at: 0 762 1701
Size: 762 939
SapI GCTCTTCn'nnn_
Cuts at: 0 40 1701
Size: 40 1661
SfcI C'TryA_G
Cuts at: 0 461 1428 1701
Size: 461 967 273
Fragments arranged by size:
967 461 273
SmlI C'TyrA_G
Cuts at: 0 379 1054 1123 1701
Size: 379 675 69 578
Fragments arranged by size:
675 578 379 69
StuI AGG'CCT
Cuts at: 0 1468 1701
Size: 1468 233
StyI C'CwwG_G
Cuts at: 0 57 188 311 1278 1701
Size: 57 131 123 967 423
Fragments arranged by size:
967 423 131 123 57
TatI w'GTAC_w
Cuts at: 0 1097 1701
Size: 1097 604
Enzymes that do cut:
AcuI AflII AhdI AleI ApoI AvrII BanI BanII
BbsI BciVI Bme1580I BmrI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BssSI BstBI BstEII BstXI Bsu36I BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoRI EcoRV Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NdeI NspI PasI PflMI
PfoI PsiI PstI PvuII SacI SapI SfcI SmlI
StuI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BseYI BsiEI BsiWI BsmI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstYI BstZ17I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI
EcoO109I FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI