Restriction map for
pF1KA0377
(Linear) (Six-base) MAPSORT of: KIAA0377.seq Check: 6353 from: 1 to: 4218
KIAA0377.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 781 2096 4218
Size: 781 1315 2122
Fragments arranged by size:
2122 1315 781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1438 1582 2697 3483 3554 4055 4218
Size: 830 608 144 1115 786 71 501 163
Fragments arranged by size:
1115 830 786 608 501 163 144 71
AfeI AGC'GCT
Cuts at: 0 3277 4218
Size: 3277 941
AflIII A'CryG_T
Cuts at: 0 2491 3525 4218
Size: 2491 1034 693
Fragments arranged by size:
2491 1034 693
AhdI GACnn_n'nnGTC
Cuts at: 0 2088 4218
Size: 2088 2130
ApaI G_GGCC'C
Cuts at: 0 2899 4218
Size: 2899 1319
ApoI r'AATT_y
Cuts at: 0 3127 3519 4218
Size: 3127 392 699
Fragments arranged by size:
3127 699 392
AvaI C'yCGr_G
Cuts at: 0 2456 2976 4218
Size: 2456 520 1242
Fragments arranged by size:
2456 1242 520
AvrII C'CTAG_G
Cuts at: 0 3042 4218
Size: 3042 1176
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1549 1582 4218
Size: 1549 33 2636
Fragments arranged by size:
2636 1549 33
BamHI G'GATC_C
Cuts at: 0 449 2673 4218
Size: 449 2224 1545
Fragments arranged by size:
2224 1545 449
BanI G'GyrC_C
Cuts at: 0 76 3066 3642 4218
Size: 76 2990 576 576
Fragments arranged by size:
2990 576 576 76
BanII G_rGCy'C
Cuts at: 0 122 2899 3341 3593 4218
Size: 122 2777 442 252 625
Fragments arranged by size:
2777 625 442 252 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 614 1402 2365 2532 4218
Size: 548 66 788 963 167 1686
Fragments arranged by size:
1686 963 788 548 167 66
BbvCI CC'TCA_GC
Cuts at: 0 1124 1604 4218
Size: 1124 480 2614
Fragments arranged by size:
2614 1124 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1027 1061 4218
Size: 1027 34 3157
Fragments arranged by size:
3157 1027 34
BclI T'GATC_A
Cuts at: 0 2025 3504 4218
Size: 2025 1479 714
Fragments arranged by size:
2025 1479 714
BglI GCCn_nnn'nGGC
Cuts at: 0 3320 4218
Size: 3320 898
BglII A'GATC_T
Cuts at: 0 1718 4218
Size: 1718 2500
BlpI GC'TnA_GC
Cuts at: 0 639 3742 4218
Size: 639 3103 476
Fragments arranged by size:
3103 639 476
Bme1580I G_kGCm'C
Cuts at: 0 79 2754 2899 4218
Size: 79 2675 145 1319
Fragments arranged by size:
2675 1319 145 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1550 2482 4218
Size: 1550 932 1736
Fragments arranged by size:
1736 1550 932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 665 809 1306 1384 1450 1661 2176
Size: 80 585 144 497 78 66 211 515
Cuts at: 2176 3253 3544 3925 4218
Size: 1077 291 381 293
Fragments arranged by size:
1077 585 515 497 381 293 291 211
144 80 78 66
Bpu10I CC'TnA_GC
Cuts at: 0 1124 1313 1604 4218
Size: 1124 189 291 2614
Fragments arranged by size:
2614 1124 291 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1288 1699 1890 3153 3333 4218
Size: 145 1143 411 191 1263 180 885
Fragments arranged by size:
1263 1143 885 411 191 180 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 2560 3559 3948 4218
Size: 2560 999 389 270
Fragments arranged by size:
2560 999 389 270
BsaAI yAC'GTr
Cuts at: 0 3241 3528 4218
Size: 3241 287 690
Fragments arranged by size:
3241 690 287
BsaBI GATnn'nnATC
Cuts at: 0 2644 4218
Size: 2644 1574
BsaWI w'CCGG_w
Cuts at: 0 446 1460 4218
Size: 446 1014 2758
Fragments arranged by size:
2758 1014 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 260 290 2815 2845 4218
Size: 260 30 2525 30 1373
Fragments arranged by size:
2525 1373 260 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 662 757 1405 1621 2356 2809 2925
Size: 148 514 95 648 216 735 453 116
Cuts at: 2925 3688 3931 4063 4189 4218
Size: 763 243 132 126 29
Fragments arranged by size:
763 735 648 514 453 243 216 148
132 126 116 95 29
BseYI C'CCAG_C
Cuts at: 0 72 634 1611 2882 3382 3410 4029 4218
Size: 72 562 977 1271 500 28 619 189
Fragments arranged by size:
1271 977 619 562 500 189 72 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1888 2257 4218
Size: 623 1265 369 1961
Fragments arranged by size:
1961 1265 623 369
BsiHKAI G_wGCw'C
Cuts at: 0 122 494 2468 3341 3585 3593 4218
Size: 122 372 1974 873 244 8 625
Fragments arranged by size:
1974 873 625 372 244 122 8
BsmI GAATG_Cn'
Cuts at: 0 526 2081 4218
Size: 526 1555 2137
Fragments arranged by size:
2137 1555 526
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1693 2290 2500 4218
Size: 667 1026 597 210 1718
Fragments arranged by size:
1718 1026 667 597 210
Bsp1286I G_dGCh'C
Cuts at: 0 79 122 494 2468 2754 2899 3341 3585
Size: 79 43 372 1974 286 145 442 244
Cuts at: 3585 3593 4218
Size: 8 625
Fragments arranged by size:
1974 625 442 372 286 244 145 79
43 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2108 2411 4218
Size: 2108 303 1807
Fragments arranged by size:
2108 1807 303
BsrDI GCAATG_nn'
Cuts at: 0 1181 2866 3787 4218
Size: 1181 1685 921 431
Fragments arranged by size:
1685 1181 921 431
BsrFI r'CCGG_y
Cuts at: 0 2993 4218
Size: 2993 1225
BssSI C'ACGA_G
Cuts at: 0 2416 4218
Size: 2416 1802
BstBI TT'CG_AA
Cuts at: 0 381 2487 4218
Size: 381 2106 1731
Fragments arranged by size:
2106 1731 381
BstEII G'GTnAC_C
Cuts at: 0 3805 4218
Size: 3805 413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1056 3382 3802 4218
Size: 1056 2326 420 416
Fragments arranged by size:
2326 1056 420 416
BstYI r'GATC_y
Cuts at: 0 449 1518 1718 2063 2201 2673 4040 4218
Size: 449 1069 200 345 138 472 1367 178
Fragments arranged by size:
1367 1069 472 449 345 200 178 138
BstZ17I GTA'TAC
Cuts at: 0 782 4218
Size: 782 3436
Bsu36I CC'TnA_GG
Cuts at: 0 515 1940 2891 4046 4172 4205 4218
Size: 515 1425 951 1155 126 33 13
Fragments arranged by size:
1425 1155 951 515 126 33 13
BtgI C'CryG_G
Cuts at: 0 35 81 896 1102 1115 2948 3062 4218
Size: 35 46 815 206 13 1833 114 1156
Fragments arranged by size:
1833 1156 815 206 114 46 35 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1871 4218
Size: 1871 2347
BtsI GCAGTG_nn'
Cuts at: 0 315 696 2754 3091 3429 4218
Size: 315 381 2058 337 338 789
Fragments arranged by size:
2058 789 381 338 337 315
DraIII CAC_nnn'GTG
Cuts at: 0 1890 4218
Size: 1890 2328
EaeI y'GGCC_r
Cuts at: 0 14 304 407 911 1590 3013 3321 4218
Size: 14 290 103 504 679 1423 308 897
Fragments arranged by size:
1423 897 679 504 308 290 103 14
EarI CTCTTCn'nnn_
Cuts at: 0 98 455 1819 2170 2787 3251 4031 4218
Size: 98 357 1364 351 617 464 780 187
Fragments arranged by size:
1364 780 617 464 357 351 187 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1693 4218
Size: 1693 2525
EcoICRI GAG'CTC
Cuts at: 0 120 3339 3591 4218
Size: 120 3219 252 627
Fragments arranged by size:
3219 627 252 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 665 809 830 1306 1384 1438 1450
Size: 80 585 144 21 476 78 54 12
Cuts at: 1450 1582 1661 2176 2697 3253 3483 3544 3554
Size: 132 79 515 521 556 230 61 10
Cuts at: 3554 3925 4055 4218
Size: 371 130 163
Fragments arranged by size:
585 556 521 515 476 371 230 163
144 132 130 80 79 78 61 54
21 12 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1773 2082 2895 2896 3699 3762 3825 4200
Size: 1773 309 813 1 803 63 63 375
Cuts at: 4200 4218
Size: 18
Fragments arranged by size:
1773 813 803 375 309 63 63 18
1
EcoRI G'AATT_C
Cuts at: 0 3127 4218
Size: 3127 1091
EcoRV GAT'ATC
Cuts at: 0 877 1338 2193 4218
Size: 877 461 855 2025
Fragments arranged by size:
2025 877 855 461
HaeII r_GCGC'y
Cuts at: 0 2187 3153 3279 3742 4218
Size: 2187 966 126 463 476
Fragments arranged by size:
2187 966 476 463 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 861 866 893 898 1425 1457 2074 2106
Size: 861 5 27 5 527 32 617 32
Cuts at: 2106 2410 2442 2665 2697 2906 2938 2956 2988
Size: 304 32 223 32 209 32 18 32
Cuts at: 2988 3469 3496 3501 3528 4141 4173 4218
Size: 481 27 5 27 613 32 45
Fragments arranged by size:
861 617 613 527 481 304 223 209
45 32 32 32 32 32 32 32
27 27 27 18 5 5 5
HincII GTy'rAC
Cuts at: 0 2433 4218
Size: 2433 1785
HindIII A'AGCT_T
Cuts at: 0 376 1499 4218
Size: 376 1123 2719
Fragments arranged by size:
2719 1123 376
MfeI C'AATT_G
Cuts at: 0 1471 4218
Size: 1471 2747
MluI A'CGCG_T
Cuts at: 0 2491 4218
Size: 2491 1727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2125 4218
Size: 2125 2093
MscI TGG'CCA
Cuts at: 0 306 409 913 3323 4218
Size: 306 103 504 2410 895
Fragments arranged by size:
2410 895 504 306 103
MslI CAynn'nnrTG
Cuts at: 0 1069 1162 1810 2420 3025 3158 3800 4218
Size: 1069 93 648 610 605 133 642 418
Fragments arranged by size:
1069 648 642 610 605 418 133 93
MspA1I CmG'CkG
Cuts at: 0 643 1416 1877 1956 4033 4218
Size: 643 773 461 79 2077 185
Fragments arranged by size:
2077 773 643 461 185 79
NcoI C'CATG_G
Cuts at: 0 896 1102 2948 3062 4218
Size: 896 206 1846 114 1156
Fragments arranged by size:
1846 1156 896 206 114
NspI r_CATG'y
Cuts at: 0 1635 3818 4218
Size: 1635 2183 400
Fragments arranged by size:
2183 1635 400
PasI CC'CwG_GG
Cuts at: 0 3571 4218
Size: 3571 647
PflMI CCAn_nnn'nTGG
Cuts at: 0 760 4218
Size: 760 3458
PfoI T'CCnGG_A
Cuts at: 0 1322 2066 2835 3560 4001 4218
Size: 1322 744 769 725 441 217
Fragments arranged by size:
1322 769 744 725 441 217
PmlI CAC'GTG
Cuts at: 0 3528 4218
Size: 3528 690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2677 2709 4218
Size: 2677 32 1509
Fragments arranged by size:
2677 1509 32
PpuMI rG'GwC_Cy
Cuts at: 0 2082 3699 3762 3825 4200 4218
Size: 2082 1617 63 63 375 18
Fragments arranged by size:
2082 1617 375 63 63 18
PshAI GACnn'nnGTC
Cuts at: 0 256 4218
Size: 256 3962
PspOMI G'GGCC_C
Cuts at: 0 2895 4218
Size: 2895 1323
PspXI vC'TCGA_Gb
Cuts at: 0 2976 4218
Size: 2976 1242
PstI C_TGCA'G
Cuts at: 0 238 458 2105 2408 3183 4190 4218
Size: 238 220 1647 303 775 1007 28
Fragments arranged by size:
1647 1007 775 303 238 220 28
PvuII CAG'CTG
Cuts at: 0 643 1416 1877 1956 4033 4218
Size: 643 773 461 79 2077 185
Fragments arranged by size:
2077 773 643 461 185 79
SacI G_AGCT'C
Cuts at: 0 122 3341 3593 4218
Size: 122 3219 252 625
Fragments arranged by size:
3219 625 252 122
SbfI CC_TGCA'GG
Cuts at: 0 458 4218
Size: 458 3760
ScaI AGT'ACT
Cuts at: 0 1974 4218
Size: 1974 2244
SexAI A'CCwGG_T
Cuts at: 0 1374 4218
Size: 1374 2844
SfcI C'TryA_G
Cuts at: 0 234 454 1915 2093 2101 2137 2404 3179
Size: 234 220 1461 178 8 36 267 775
Cuts at: 3179 3470 4186 4218
Size: 291 716 32
Fragments arranged by size:
1461 775 716 291 267 234 220 178
36 32 8
SmlI C'TyrA_G
Cuts at: 0 124 1303 1714 1869 2976 3132 3348 4218
Size: 124 1179 411 155 1107 156 216 870
Fragments arranged by size:
1179 1107 870 411 216 156 155 124
StuI AGG'CCT
Cuts at: 0 2982 3214 3548 4218
Size: 2982 232 334 670
Fragments arranged by size:
2982 670 334 232
StyI C'CwwG_G
Cuts at: 0 51 896 1102 1246 1769 2811 2948 3042
Size: 51 845 206 144 523 1042 137 94
Cuts at: 3042 3062 3292 3549 4218
Size: 20 230 257 669
Fragments arranged by size:
1042 845 669 523 257 230 206 144
137 94 51 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1259 1818 2071 3033 3424 4218
Size: 1259 559 253 962 391 794
Fragments arranged by size:
1259 962 794 559 391 253
TatI w'GTAC_w
Cuts at: 0 1425 1972 2450 4218
Size: 1425 547 478 1768
Fragments arranged by size:
1768 1425 547 478
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2658 2690 2815 2847 4218
Size: 2658 32 125 32 1371
Fragments arranged by size:
2658 1371 125 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 726 2221 4218
Size: 726 1495 1997
Fragments arranged by size:
1997 1495 726
XbaI T'CTAG_A
Cuts at: 0 1785 4218
Size: 1785 2433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 49 582 903 1776 3081 3568 3985 4218
Size: 49 533 321 873 1305 487 417 233
Fragments arranged by size:
1305 873 533 487 417 321 233 49
XhoI C'TCGA_G
Cuts at: 0 2976 4218
Size: 2976 1242
XmnI GAAnn'nnTTC
Cuts at: 0 1974 4218
Size: 1974 2244
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI ApaI ApoI AvaI
AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII MfeI
MluI MmeI MscI MslI MspA1I NcoI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SbfI ScaI SexAI SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI BbeI BciVI
BfrBI BmgBI BmrI BmtI BsaHI BsiEI BsiWI BspEI
BspHI BsrBI BsrGI BssHII BstAPI ClaI CspCI DraI
DrdI EagI EcoNI FalI FseI FspI FspAI HpaI
KasI KpnI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PsiI PsrI PvuI
RsrII SacII SalI SanDI SapI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XmaI ZraI