Restriction map for
pF1KSDA0375
(Linear) (Six-base) MAPSORT of: KIAA0375.seq Check: 9744 from: 1 to: 4548
KIAA0375.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 88 404 4548
Size: 88 316 4144
Fragments arranged by size:
4144 316 88
AatII G_ACGT'C
Cuts at: 0 1479 3176 4548
Size: 1479 1697 1372
Fragments arranged by size:
1697 1479 1372
AccI GT'mk_AC
Cuts at: 0 1439 3839 4548
Size: 1439 2400 709
Fragments arranged by size:
2400 1439 709
Acc65I G'GTAC_C
Cuts at: 0 3812 4548
Size: 3812 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 2956 3130 3486 4548
Size: 834 2122 174 356 1062
Fragments arranged by size:
2122 1062 834 356 174
AfeI AGC'GCT
Cuts at: 0 1483 4548
Size: 1483 3065
AleI CACnn'nnGTG
Cuts at: 0 1880 4548
Size: 1880 2668
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 678 710 4548
Size: 678 32 3838
Fragments arranged by size:
3838 678 32
AlwNI CAG_nnn'CTG
Cuts at: 0 566 949 1129 1297 1405 1408 1951 3486
Size: 566 383 180 168 108 3 543 1535
Cuts at: 3486 3591 4362 4548
Size: 105 771 186
Fragments arranged by size:
1535 771 566 543 383 186 180 168
108 105 3
ApaI G_GGCC'C
Cuts at: 0 1417 1582 1755 2136 2372 3633 3649 3789
Size: 1417 165 173 381 236 1261 16 140
Cuts at: 3789 4094 4548
Size: 305 454
Fragments arranged by size:
1417 1261 454 381 305 236 173 165
140 16
ApaLI G'TGCA_C
Cuts at: 0 58 4548
Size: 58 4490
AvaI C'yCGr_G
Cuts at: 0 2129 2264 2612 3049 3626 3632 3788 3950
Size: 2129 135 348 437 577 6 156 162
Cuts at: 3950 4548
Size: 598
Fragments arranged by size:
2129 598 577 437 348 162 156 135
6
AvrII C'CTAG_G
Cuts at: 0 174 4548
Size: 174 4374
BamHI G'GATC_C
Cuts at: 0 1941 3045 4548
Size: 1941 1104 1503
Fragments arranged by size:
1941 1503 1104
BanI G'GyrC_C
Cuts at: 0 556 877 952 2020 2281 3697 3812 4485
Size: 556 321 75 1068 261 1416 115 673
Cuts at: 4485 4548
Size: 63
Fragments arranged by size:
1416 1068 673 556 321 261 115 75
63
BanII G_rGCy'C
Cuts at: 0 288 1417 1537 1582 1755 1969 2136 2372
Size: 288 1129 120 45 173 214 167 236
Cuts at: 2372 2575 3088 3155 3293 3633 3649 3769 3789
Size: 203 513 67 138 340 16 120 20
Cuts at: 3789 3832 4042 4094 4295 4548
Size: 43 210 52 201 253
Fragments arranged by size:
1129 513 340 288 253 236 214 210
203 201 173 167 138 120 120 67
52 45 43 20 16
BbeI G_GCGC'C
Cuts at: 0 956 4548
Size: 956 3592
BbsI GAAGACnn'nnnn_
Cuts at: 0 3392 4548
Size: 3392 1156
BbvCI CC'TCA_GC
Cuts at: 0 1465 2590 3974 4066 4548
Size: 1465 1125 1384 92 482
Fragments arranged by size:
1465 1384 1125 482 92
BciVI GTATCCnnnnn_n'
Cuts at: 0 534 4548
Size: 534 4014
BfrBI ATG'CAT
Cuts at: 0 873 4548
Size: 873 3675
BglI GCCn_nnn'nGGC
Cuts at: 0 1238 1639 1806 2168 2200 3644 3784 4548
Size: 1238 401 167 362 32 1444 140 764
Fragments arranged by size:
1444 1238 764 401 362 167 140 32
BlpI GC'TnA_GC
Cuts at: 0 1931 4228 4396 4548
Size: 1931 2297 168 152
Fragments arranged by size:
2297 1931 168 152
Bme1580I G_kGCm'C
Cuts at: 0 62 1120 1417 1582 1755 1771 2136 2372
Size: 62 1058 297 165 173 16 365 236
Cuts at: 2372 3124 3633 3649 3789 4094 4490 4548
Size: 752 509 16 140 305 396 58
Fragments arranged by size:
1058 752 509 396 365 305 297 236
173 165 140 62 58 16 16
BmgBI CAC'GTC
Cuts at: 0 3657 4548
Size: 3657 891
BmrI ACTGGGnnnn_n'
Cuts at: 0 1555 1922 2642 4548
Size: 1555 367 720 1906
Fragments arranged by size:
1906 1555 720 367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 49 3449 4335 4548
Size: 49 3400 886 213
Fragments arranged by size:
3400 886 213 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 203 302 518 1682 1863 2897 3163
Size: 44 159 99 216 1164 181 1034 266
Cuts at: 3163 3373 3671 4163 4320 4460 4548
Size: 210 298 492 157 140 88
Fragments arranged by size:
1164 1034 492 298 266 216 210 181
159 157 140 99 88 44
Bpu10I CC'TnA_GC
Cuts at: 0 143 732 1070 1465 2590 3768 3974 4066
Size: 143 589 338 395 1125 1178 206 92
Cuts at: 4066 4548
Size: 482
Fragments arranged by size:
1178 1125 589 482 395 338 206 143
92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1447 1590 2201 2657 2665 3139 4548
Size: 1447 143 611 456 8 474 1409
Fragments arranged by size:
1447 1409 611 474 456 143 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 22 2302 4548
Size: 22 2280 2246
Fragments arranged by size:
2280 2246 22
BsaAI yAC'GTr
Cuts at: 0 4500 4548
Size: 4500 48
BsaHI Gr'CG_yC
Cuts at: 0 953 1476 3173 4548
Size: 953 523 1697 1375
Fragments arranged by size:
1697 1375 953 523
BsaWI w'CCGG_w
Cuts at: 0 2348 3344 4548
Size: 2348 996 1204
Fragments arranged by size:
2348 1204 996
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 621 651 1075 1105 3346 3376 4500 4530
Size: 621 30 424 30 2241 30 1124 30
Cuts at: 4530 4548
Size: 18
Fragments arranged by size:
2241 1124 621 424 30 30 30 30
18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 804 1695 3748 4009 4457 4548
Size: 804 891 2053 261 448 91
Fragments arranged by size:
2053 891 804 448 261 91
BseYI C'CCAG_C
Cuts at: 0 147 158 380 1119 1315 1736 1968 2150
Size: 147 11 222 739 196 421 232 182
Cuts at: 2150 2229 2293 2371 2489 3066 4184 4323 4428
Size: 79 64 78 118 577 1118 139 105
Cuts at: 4428 4548
Size: 120
Fragments arranged by size:
1118 739 577 421 232 222 196 182
147 139 120 118 105 79 78 64
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 215 3449 4375 4548
Size: 193 22 3234 926 173
Fragments arranged by size:
3234 926 193 173 22
BsiHKAI G_wGCw'C
Cuts at: 0 62 118 2404 3293 3832 4548
Size: 62 56 2286 889 539 716
Fragments arranged by size:
2286 889 716 539 62 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3647 4548
Size: 3647 901
Bsp1286I G_dGCh'C
Cuts at: 0 62 118 288 1120 1417 1537 1582 1755
Size: 62 56 170 832 297 120 45 173
Cuts at: 1755 1771 1969 2136 2372 2404 2575 3088 3124
Size: 16 198 167 236 32 171 513 36
Cuts at: 3124 3155 3293 3633 3649 3769 3789 3832 4042
Size: 31 138 340 16 120 20 43 210
Cuts at: 4042 4094 4295 4490 4548
Size: 52 201 195 58
Fragments arranged by size:
832 513 340 297 236 210 201 198
195 173 171 170 167 138 120 120
62 58 56 52 45 43 36 32
31 20 16 16
BspEI T'CCGG_A
Cuts at: 0 2348 4548
Size: 2348 2200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 88 404 2878 2975 3599 4548
Size: 88 316 2474 97 624 949
Fragments arranged by size:
2474 949 624 316 97 88
BsrBI CCG'CTC
Cuts at: 0 3681 4548
Size: 3681 867
BsrFI r'CCGG_y
Cuts at: 0 572 2537 2831 3527 3802 4381 4548
Size: 572 1965 294 696 275 579 167
Fragments arranged by size:
1965 696 579 572 294 275 167
BssSI C'ACGA_G
Cuts at: 0 3875 4548
Size: 3875 673
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1405 3531 4548
Size: 1405 2126 1017
Fragments arranged by size:
2126 1405 1017
BstEII G'GTnAC_C
Cuts at: 0 343 843 1620 4548
Size: 343 500 777 2928
Fragments arranged by size:
2928 777 500 343
BstXI CCAn_nnnn'nTGG
Cuts at: 0 400 1803 2118 4548
Size: 400 1403 315 2430
Fragments arranged by size:
2430 1403 400 315
BstYI r'GATC_y
Cuts at: 0 966 1941 3045 4548
Size: 966 975 1104 1503
Fragments arranged by size:
1503 1104 975 966
BstZ17I GTA'TAC
Cuts at: 0 1440 4548
Size: 1440 3108
Bsu36I CC'TnA_GG
Cuts at: 0 2238 4548
Size: 2238 2310
BtgI C'CryG_G
Cuts at: 0 472 794 1589 2446 2544 3202 4464 4548
Size: 472 322 795 857 98 658 1262 84
Fragments arranged by size:
1262 857 795 658 472 322 98 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2306 2988 4548
Size: 2306 682 1560
Fragments arranged by size:
2306 1560 682
BtsI GCAGTG_nn'
Cuts at: 0 60 88 556 564 642 744 1130 1385
Size: 60 28 468 8 78 102 386 255
Cuts at: 1385 2071 2855 4548
Size: 686 784 1693
Fragments arranged by size:
1693 784 686 468 386 255 102 78
60 28 8
ClaI AT'CG_AT
Cuts at: 0 3845 4548
Size: 3845 703
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1407 1442 2708 2743 4548
Size: 1407 35 1266 35 1805
Fragments arranged by size:
1805 1407 1266 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1108 1687 2399 4548
Size: 1108 579 712 2149
Fragments arranged by size:
2149 1108 712 579
EaeI y'GGCC_r
Cuts at: 0 408 574 1172 1592 1640 2120 2317 2636
Size: 408 166 598 420 48 480 197 319
Cuts at: 2636 2841 4058 4376 4548
Size: 205 1217 318 172
Fragments arranged by size:
1217 598 480 420 408 319 318 205
197 172 166 48
EarI CTCTTCn'nnn_
Cuts at: 0 267 930 1068 1248 2388 3455 3710 3716
Size: 267 663 138 180 1140 1067 255 6
Cuts at: 3716 3725 3737 4548
Size: 9 12 811
Fragments arranged by size:
1140 1067 811 663 267 255 180 138
12 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1516 4548
Size: 1516 3032
EcoICRI GAG'CTC
Cuts at: 0 3291 3830 4548
Size: 3291 539 718
Fragments arranged by size:
3291 718 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 203 302 518 834 1682 1863 2897
Size: 44 159 99 216 316 848 181 1034
Cuts at: 2897 2956 3130 3163 3373 3486 3671 4163 4320
Size: 59 174 33 210 113 185 492 157
Cuts at: 4320 4460 4548
Size: 140 88
Fragments arranged by size:
1034 848 492 316 216 210 185 181
174 159 157 140 113 99 88 59
44 33
EcoO109I rG'GnC_Cy
Cuts at: 0 277 619 1294 1312 1414 1578 1579 1751
Size: 277 342 675 18 102 164 1 172
Cuts at: 1751 1752 1870 1921 1948 2133 2886 3249 3588
Size: 1 118 51 27 185 753 363 339
Cuts at: 3588 3645 3646 3928 3999 4090 4124 4548
Size: 57 1 282 71 91 34 424
Fragments arranged by size:
753 675 424 363 342 339 282 277
185 172 164 118 102 91 71 57
51 34 27 18 1 1 1
HaeII r_GCGC'y
Cuts at: 0 956 1485 4548
Size: 956 529 3063
Fragments arranged by size:
3063 956 529
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 2573 2605 2734 2766 2929 2961
Size: 246 32 2295 32 129 32 163 32
Cuts at: 2961 4059 4091 4500 4532 4548
Size: 1098 32 409 32 16
Fragments arranged by size:
2295 1098 409 246 163 129 32 32
32 32 32 32 16
HincII GTy'rAC
Cuts at: 0 3000 4548
Size: 3000 1548
HindIII A'AGCT_T
Cuts at: 0 2556 4548
Size: 2556 1992
HpaI GTT'AAC
Cuts at: 0 3000 4548
Size: 3000 1548
KasI G'GCGC_C
Cuts at: 0 952 4548
Size: 952 3596
KpnI G_GTAC'C
Cuts at: 0 3816 4548
Size: 3816 732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4275 4548
Size: 4275 273
MscI TGG'CCA
Cuts at: 0 410 1174 1642 2122 2638 2843 4378 4548
Size: 410 764 468 480 516 205 1535 170
Fragments arranged by size:
1535 764 516 480 468 410 205 170
MslI CAynn'nnrTG
Cuts at: 0 398 1880 3007 4548
Size: 398 1482 1127 1541
Fragments arranged by size:
1541 1482 1127 398
MspA1I CmG'CkG
Cuts at: 0 441 752 784 1405 1935 2118 2917 3555
Size: 441 311 32 621 530 183 799 638
Cuts at: 3555 3621 4096 4188 4395 4548
Size: 66 475 92 207 153
Fragments arranged by size:
799 638 621 530 475 441 311 207
183 153 92 66 32
NaeI GCC'GGC
Cuts at: 0 574 2833 3804 4548
Size: 574 2259 971 744
Fragments arranged by size:
2259 971 744 574
NarI GG'CG_CC
Cuts at: 0 953 4548
Size: 953 3595
NcoI C'CATG_G
Cuts at: 0 472 794 1589 2446 2544 3202 4548
Size: 472 322 795 857 98 658 1346
Fragments arranged by size:
1346 857 795 658 472 322 98
NgoMIV G'CCGG_C
Cuts at: 0 572 2831 3802 4548
Size: 572 2259 971 746
Fragments arranged by size:
2259 971 746 572
NsiI A_TGCA'T
Cuts at: 0 875 4548
Size: 875 3673
NspI r_CATG'y
Cuts at: 0 3201 4548
Size: 3201 1347
PasI CC'CwG_GG
Cuts at: 0 1960 2328 3413 4002 4548
Size: 1960 368 1085 589 546
Fragments arranged by size:
1960 1085 589 546 368
PflMI CCAn_nnn'nTGG
Cuts at: 0 340 451 795 1297 4257 4374 4531 4548
Size: 340 111 344 502 2960 117 157 17
Fragments arranged by size:
2960 502 344 340 157 117 111 17
PfoI T'CCnGG_A
Cuts at: 0 219 2882 4548
Size: 219 2663 1666
Fragments arranged by size:
2663 1666 219
PpuMI rG'GwC_Cy
Cuts at: 0 619 1870 2886 3249 3588 3928 4548
Size: 619 1251 1016 363 339 340 620
Fragments arranged by size:
1251 1016 620 619 363 340 339
PshAI GACnn'nnGTC
Cuts at: 0 1838 4548
Size: 1838 2710
PspOMI G'GGCC_C
Cuts at: 0 1413 1578 1751 2132 2368 3629 3645 3785
Size: 1413 165 173 381 236 1261 16 140
Cuts at: 3785 4090 4548
Size: 305 458
Fragments arranged by size:
1413 1261 458 381 305 236 173 165
140 16
PstI C_TGCA'G
Cuts at: 0 317 757 919 937 974 1511 1587 2176
Size: 317 440 162 18 37 537 76 589
Cuts at: 2176 3620 4310 4462 4548
Size: 1444 690 152 86
Fragments arranged by size:
1444 690 589 537 440 317 162 152
86 76 37 18
PvuII CAG'CTG
Cuts at: 0 441 784 1405 1935 2118 2917 3621 4395
Size: 441 343 621 530 183 799 704 774
Cuts at: 4395 4548
Size: 153
Fragments arranged by size:
799 774 704 621 530 441 343 183
153
SacI G_AGCT'C
Cuts at: 0 3293 3832 4548
Size: 3293 539 716
Fragments arranged by size:
3293 716 539
SanDI GG'GwC_CC
Cuts at: 0 1870 4548
Size: 1870 2678
SapI GCTCTTCn'nnn_
Cuts at: 0 1248 3455 4548
Size: 1248 2207 1093
Fragments arranged by size:
2207 1248 1093
SbfI CC_TGCA'GG
Cuts at: 0 4310 4548
Size: 4310 238
ScaI AGT'ACT
Cuts at: 0 3115 4548
Size: 3115 1433
SexAI A'CCwGG_T
Cuts at: 0 621 2721 3101 4548
Size: 621 2100 380 1447
Fragments arranged by size:
2100 1447 621 380
SfcI C'TryA_G
Cuts at: 0 239 313 753 915 933 970 1507 1583
Size: 239 74 440 162 18 37 537 76
Cuts at: 1583 1776 2046 2157 2172 3478 3616 4306 4458
Size: 193 270 111 15 1306 138 690 152
Cuts at: 4458 4548
Size: 90
Fragments arranged by size:
1306 690 537 440 270 239 193 162
152 138 111 90 76 74 37 18
15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3644 4548
Size: 3644 904
SfoI GGC'GCC
Cuts at: 0 954 4548
Size: 954 3594
SmaI CCC'GGG
Cuts at: 0 2131 2614 3051 3628 3634 4548
Size: 2131 483 437 577 6 914
Fragments arranged by size:
2131 914 577 483 437 6
SmlI C'TyrA_G
Cuts at: 0 1426 1569 2216 2672 2680 3154 4548
Size: 1426 143 647 456 8 474 1394
Fragments arranged by size:
1426 1394 647 474 456 143 8
SpeI A'CTAG_T
Cuts at: 0 4254 4548
Size: 4254 294
SrfI GCCC'GGGC
Cuts at: 0 2131 3628 4548
Size: 2131 1497 920
Fragments arranged by size:
2131 1497 920
StuI AGG'CCT
Cuts at: 0 3160 3979 4548
Size: 3160 819 569
Fragments arranged by size:
3160 819 569
StyI C'CwwG_G
Cuts at: 0 174 472 536 709 794 1589 1643 2123
Size: 174 298 64 173 85 795 54 480
Cuts at: 2123 2446 2544 3202 3245 3672 4295 4548
Size: 323 98 658 43 427 623 253
Fragments arranged by size:
795 658 623 480 427 323 298 253
174 173 98 85 64 54 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 316 394 1408 1626 1737 1809 1870
Size: 172 144 78 1014 218 111 72 61
Cuts at: 1870 2759 3685 4548
Size: 889 926 863
Fragments arranged by size:
1014 926 889 863 218 172 144 111
78 72 61
TatI w'GTAC_w
Cuts at: 0 3113 3165 4548
Size: 3113 52 1383
Fragments arranged by size:
3113 1383 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 621 653 2063 2095 2131 2163 4548
Size: 621 32 1410 32 36 32 2385
Fragments arranged by size:
2385 1410 621 36 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 946 1844 3618 3646 4548
Size: 946 898 1774 28 902
Fragments arranged by size:
1774 946 902 898 28
XmaI C'CCGG_G
Cuts at: 0 2129 2612 3049 3626 3632 4548
Size: 2129 483 437 577 6 916
Fragments arranged by size:
2129 916 577 483 437 6
XmnI GAAnn'nnTTC
Cuts at: 0 3075 4548
Size: 3075 1473
ZraI GAC'GTC
Cuts at: 0 1477 3174 4548
Size: 1477 1697 1374
Fragments arranged by size:
1697 1477 1374
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AleI AloI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII HindIII HpaI KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SpeI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AlfI ApoI AscI
AseI AsiSI BaeI BcgI BclI BglII BmtI BsaBI
BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstBI
DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI MfeI MluI NdeI NheI NotI NruI
PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SalI SgfI SgrAI SnaBI SphI
SspI SwaI Tth111I XbaI XhoI