Restriction map for
pF1KSDA0372
(Linear) (Six-base) MAPSORT of: KIAA0372.seq Check: 4289 from: 1 to: 4692
KIAA0372.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 2078 2320 4278 4692
Size: 2078 242 1958 414
Fragments arranged by size:
2078 1958 414 242
Acc65I G'GTAC_C
Cuts at: 0 4571 4692
Size: 4571 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1088 1280 1468 2733 2801 3466 4049
Size: 1018 70 192 188 1265 68 665 583
Cuts at: 4049 4692
Size: 643
Fragments arranged by size:
1265 1018 665 643 583 192 188 70
68
AflII C'TTAA_G
Cuts at: 0 1824 2407 4692
Size: 1824 583 2285
Fragments arranged by size:
2285 1824 583
AflIII A'CryG_T
Cuts at: 0 4221 4692
Size: 4221 471
AhdI GACnn_n'nnGTC
Cuts at: 0 4505 4692
Size: 4505 187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2836 4692
Size: 2836 1856
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1151 1182 1183 1214 1873 1905 4692
Size: 1151 31 1 31 659 32 2787
Fragments arranged by size:
2787 1151 659 32 31 31 1
AlwNI CAG_nnn'CTG
Cuts at: 0 498 1753 4150 4215 4692
Size: 498 1255 2397 65 477
Fragments arranged by size:
2397 1255 498 477 65
ApoI r'AATT_y
Cuts at: 0 640 2924 3943 4053 4692
Size: 640 2284 1019 110 639
Fragments arranged by size:
2284 1019 640 639 110
AseI AT'TA_AT
Cuts at: 0 2351 2576 4692
Size: 2351 225 2116
Fragments arranged by size:
2351 2116 225
AvaI C'yCGr_G
Cuts at: 0 3599 4692
Size: 3599 1093
AvrII C'CTAG_G
Cuts at: 0 1932 4692
Size: 1932 2760
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1340 1373 4210 4243 4555 4588 4692
Size: 1340 33 2837 33 312 33 104
Fragments arranged by size:
2837 1340 312 104 33 33 33
BanI G'GyrC_C
Cuts at: 0 3482 4571 4692
Size: 3482 1089 121
Fragments arranged by size:
3482 1089 121
BanII G_rGCy'C
Cuts at: 0 1338 1539 2273 4692
Size: 1338 201 734 2419
Fragments arranged by size:
2419 1338 734 201
BbsI GAAGACnn'nnnn_
Cuts at: 0 25 1048 1235 3047 3161 4692
Size: 25 1023 187 1812 114 1531
Fragments arranged by size:
1812 1531 1023 187 114 25
BbvCI CC'TCA_GC
Cuts at: 0 2714 4692
Size: 2714 1978
BciVI GTATCCnnnnn_n'
Cuts at: 0 722 1458 4550 4692
Size: 722 736 3092 142
Fragments arranged by size:
3092 736 722 142
BclI T'GATC_A
Cuts at: 0 168 1128 1970 4071 4380 4692
Size: 168 960 842 2101 309 312
Fragments arranged by size:
2101 960 842 312 309 168
BfrBI ATG'CAT
Cuts at: 0 957 2775 3004 4692
Size: 957 1818 229 1688
Fragments arranged by size:
1818 1688 957 229
BglI GCCn_nnn'nGGC
Cuts at: 0 827 1276 4692
Size: 827 449 3416
Fragments arranged by size:
3416 827 449
BlpI GC'TnA_GC
Cuts at: 0 2537 4692
Size: 2537 2155
BmgBI CAC'GTC
Cuts at: 0 4620 4692
Size: 4620 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 577 4692
Size: 577 4115
BmtI G_CTAG'C
Cuts at: 0 2158 4692
Size: 2158 2534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1610 2440 2854 3782 4144 4692
Size: 1610 830 414 928 362 548
Fragments arranged by size:
1610 928 830 548 414 362
Bpu10I CC'TnA_GC
Cuts at: 0 275 831 1702 2714 4692
Size: 275 556 871 1012 1978
Fragments arranged by size:
1978 1012 871 556 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 1360 1727 4305 4692
Size: 1294 66 367 2578 387
Fragments arranged by size:
2578 1294 387 367 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 3593 3986 4692
Size: 3593 393 706
Fragments arranged by size:
3593 706 393
BsaBI GATnn'nnATC
Cuts at: 0 691 4692
Size: 691 4001
BsaHI Gr'CG_yC
Cuts at: 0 4506 4692
Size: 4506 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3629 3659 4692
Size: 3629 30 1033
Fragments arranged by size:
3629 1033 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1847 2263 4692
Size: 1847 416 2429
Fragments arranged by size:
2429 1847 416
BseYI C'CCAG_C
Cuts at: 0 2158 2940 3820 4692
Size: 2158 782 880 872
Fragments arranged by size:
2158 880 872 782
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 897 2796 3746 4029 4547 4692
Size: 470 427 1899 950 283 518 145
Fragments arranged by size:
1899 950 518 470 427 283 145
BsiHKAI G_wGCw'C
Cuts at: 0 1338 1539 2118 2273 3390 4692
Size: 1338 201 579 155 1117 1302
Fragments arranged by size:
1338 1302 1117 579 201 155
BsiWI C'GTAC_G
Cuts at: 0 1120 4692
Size: 1120 3572
BsmI GAATG_Cn'
Cuts at: 0 567 1737 2026 2490 2883 4692
Size: 567 1170 289 464 393 1809
Fragments arranged by size:
1809 1170 567 464 393 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1505 4692
Size: 1505 3187
Bsp1286I G_dGCh'C
Cuts at: 0 1338 1539 2118 2273 3390 4692
Size: 1338 201 579 155 1117 1302
Fragments arranged by size:
1338 1302 1117 579 201 155
BspHI T'CATG_A
Cuts at: 0 648 2640 3185 4692
Size: 648 1992 545 1507
Fragments arranged by size:
1992 1507 648 545
BsrDI GCAATG_nn'
Cuts at: 0 2818 3325 3407 4692
Size: 2818 507 82 1285
Fragments arranged by size:
2818 1285 507 82
BsrGI T'GTAC_A
Cuts at: 0 705 3701 4692
Size: 705 2996 991
Fragments arranged by size:
2996 991 705
BssSI C'ACGA_G
Cuts at: 0 1538 4692
Size: 1538 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3001 3616 4345 4561 4692
Size: 3001 615 729 216 131
Fragments arranged by size:
3001 729 615 216 131
BstEII G'GTnAC_C
Cuts at: 0 3571 4692
Size: 3571 1121
BstYI r'GATC_y
Cuts at: 0 312 369 2742 4692
Size: 312 57 2373 1950
Fragments arranged by size:
2373 1950 312 57
BtgI C'CryG_G
Cuts at: 0 2738 4692
Size: 2738 1954
BtsI GCAGTG_nn'
Cuts at: 0 1754 2456 3528 3704 4216 4692
Size: 1754 702 1072 176 512 476
Fragments arranged by size:
1754 1072 702 512 476 176
DraI TTT'AAA
Cuts at: 0 2006 3336 4058 4692
Size: 2006 1330 722 634
Fragments arranged by size:
2006 1330 722 634
EaeI y'GGCC_r
Cuts at: 0 4387 4692
Size: 4387 305
EarI CTCTTCn'nnn_
Cuts at: 0 455 1107 1245 1400 2752 4692
Size: 455 652 138 155 1352 1940
Fragments arranged by size:
1940 1352 652 455 155 138
EcoICRI GAG'CTC
Cuts at: 0 1336 1537 2271 4692
Size: 1336 201 734 2421
Fragments arranged by size:
2421 1336 734 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1088 1280 1468 1610 2440 2733 2801
Size: 1018 70 192 188 142 830 293 68
Cuts at: 2801 2854 3466 3782 4049 4144 4692
Size: 53 612 316 267 95 548
Fragments arranged by size:
1018 830 612 548 316 293 267 192
188 142 95 70 68 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1701 4692
Size: 1701 2991
EcoRI G'AATT_C
Cuts at: 0 2924 4692
Size: 2924 1768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 994 3448 4449 4692
Size: 300 694 2454 1001 243
Fragments arranged by size:
2454 1001 694 300 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 1084 1116 1222 1254 1570 1602
Size: 32 32 1020 32 106 32 316 32
Cuts at: 1602 4491 4523 4692
Size: 2889 32 169
Fragments arranged by size:
2889 1020 316 169 106 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 330 1605 3134 4692
Size: 330 1275 1529 1558
Fragments arranged by size:
1558 1529 1275 330
HindIII A'AGCT_T
Cuts at: 0 71 1176 4426 4460 4692
Size: 71 1105 3250 34 232
Fragments arranged by size:
3250 1105 232 71 34
KpnI G_GTAC'C
Cuts at: 0 4575 4692
Size: 4575 117
MfeI C'AATT_G
Cuts at: 0 538 1794 4282 4692
Size: 538 1256 2488 410
Fragments arranged by size:
2488 1256 538 410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2703 4208 4285 4692
Size: 2703 1505 77 407
Fragments arranged by size:
2703 1505 407 77
MscI TGG'CCA
Cuts at: 0 4389 4692
Size: 4389 303
MslI CAynn'nnrTG
Cuts at: 0 269 3845 4589 4692
Size: 269 3576 744 103
Fragments arranged by size:
3576 744 269 103
MspA1I CmG'CkG
Cuts at: 0 151 4692
Size: 151 4541
NcoI C'CATG_G
Cuts at: 0 2738 4692
Size: 2738 1954
NheI G'CTAG_C
Cuts at: 0 2154 4692
Size: 2154 2538
NsiI A_TGCA'T
Cuts at: 0 959 2777 3006 4692
Size: 959 1818 229 1686
Fragments arranged by size:
1818 1686 959 229
NspI r_CATG'y
Cuts at: 0 2507 4225 4692
Size: 2507 1718 467
Fragments arranged by size:
2507 1718 467
PciI A'CATG_T
Cuts at: 0 4221 4692
Size: 4221 471
PfoI T'CCnGG_A
Cuts at: 0 308 4121 4692
Size: 308 3813 571
Fragments arranged by size:
3813 571 308
PsiI TTA'TAA
Cuts at: 0 3200 4692
Size: 3200 1492
PstI C_TGCA'G
Cuts at: 0 150 1599 3039 4257 4692
Size: 150 1449 1440 1218 435
Fragments arranged by size:
1449 1440 1218 435 150
PvuII CAG'CTG
Cuts at: 0 151 4692
Size: 151 4541
SacI G_AGCT'C
Cuts at: 0 1338 1539 2273 4692
Size: 1338 201 734 2419
Fragments arranged by size:
2419 1338 734 201
SapI GCTCTTCn'nnn_
Cuts at: 0 455 4692
Size: 455 4237
ScaI AGT'ACT
Cuts at: 0 608 4692
Size: 608 4084
SfcI C'TryA_G
Cuts at: 0 146 1595 1952 2119 2759 3035 4253 4692
Size: 146 1449 357 167 640 276 1218 439
Fragments arranged by size:
1449 1218 640 439 357 276 167 146
SmlI C'TyrA_G
Cuts at: 0 1273 1339 1742 1824 2407 3599 4320 4692
Size: 1273 66 403 82 583 1192 721 372
Fragments arranged by size:
1273 1192 721 583 403 372 82 66
SspI AAT'ATT
Cuts at: 0 2104 4692
Size: 2104 2588
StuI AGG'CCT
Cuts at: 0 673 821 4420 4692
Size: 673 148 3599 272
Fragments arranged by size:
3599 673 272 148
StyI C'CwwG_G
Cuts at: 0 1932 2280 2343 2738 3513 4236 4692
Size: 1932 348 63 395 775 723 456
Fragments arranged by size:
1932 775 723 456 395 348 63
SwaI ATTT'AAAT
Cuts at: 0 3336 4692
Size: 3336 1356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1390 2005 4692
Size: 1390 615 2687
Fragments arranged by size:
2687 1390 615
TatI w'GTAC_w
Cuts at: 0 508 606 705 2858 3701 4152 4692
Size: 508 98 99 2153 843 451 540
Fragments arranged by size:
2153 843 540 508 451 99 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4208 4692
Size: 4208 484
XhoI C'TCGA_G
Cuts at: 0 3599 4692
Size: 3599 1093
XmnI GAAnn'nnTTC
Cuts at: 0 464 974 1115 1232 1858 2924 3371 3735
Size: 464 510 141 117 626 1066 447 364
Cuts at: 3735 4692
Size: 957
Fragments arranged by size:
1066 957 626 510 464 447 364 141
117
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AlfI AloI
AlwNI ApoI AseI AvaI AvrII BaeI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspHI BsrDI BsrGI BssSI BstAPI BstEII BstYI
BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoRI FalI Hin4I HincII HindIII KpnI MfeI MmeI
MscI MslI MspA1I NcoI NheI NsiI NspI PciI
PfoI PsiI PstI PvuII SacI SapI ScaI SfcI
SmlI SspI StuI StyI SwaI TaqII TatI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AleI ApaI ApaLI
AscI AsiSI BamHI BbeI BcgI BglII Bme1580I BplI
BsaAI BsaWI BsiEI BspEI BspMI BsrBI BsrFI BssHII
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoO109I EcoRV FseI FspI FspAI
HaeII HpaI KasI MluI NaeI NarI NdeI NgoMIV
NotI NruI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI TstI Tth111I XbaI
XmaI ZraI