Restriction map for
pF1KA0366
(Linear) (Six-base) MAPSORT of: KIAA0366.seq Check: 3080 from: 1 to: 3615
KIAA0366.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AatII G_ACGT'C
Cuts at: 0 3588 3615
Size: 3588 27
AccI GT'mk_AC
Cuts at: 0 599 3615
Size: 599 3016
Acc65I G'GTAC_C
Cuts at: 0 2180 3615
Size: 2180 1435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 1818 2785 3305 3615
Size: 38 1780 967 520 310
Fragments arranged by size:
1780 967 520 310 38
AflII C'TTAA_G
Cuts at: 0 2185 3615
Size: 2185 1430
AflIII A'CryG_T
Cuts at: 0 215 635 1696 2217 3615
Size: 215 420 1061 521 1398
Fragments arranged by size:
1398 1061 521 420 215
AleI CACnn'nnGTG
Cuts at: 0 1728 1845 3615
Size: 1728 117 1770
Fragments arranged by size:
1770 1728 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 1121 2053 3123 3615
Size: 943 178 932 1070 492
Fragments arranged by size:
1070 943 932 492 178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1694 1726 2916 2948 3615
Size: 1694 32 1190 32 667
Fragments arranged by size:
1694 1190 667 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 491 720 2654 3026 3615
Size: 491 229 1934 372 589
Fragments arranged by size:
1934 589 491 372 229
ApaLI G'TGCA_C
Cuts at: 0 1476 2833 3615
Size: 1476 1357 782
Fragments arranged by size:
1476 1357 782
ApoI r'AATT_y
Cuts at: 0 869 1675 3615
Size: 869 806 1940
Fragments arranged by size:
1940 869 806
AvaI C'yCGr_G
Cuts at: 0 2863 3615
Size: 2863 752
BanI G'GyrC_C
Cuts at: 0 345 2180 2818 3615
Size: 345 1835 638 797
Fragments arranged by size:
1835 797 638 345
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 2240 2374 2489 3615
Size: 212 2028 134 115 1126
Fragments arranged by size:
2028 1126 212 134 115
BclI T'GATC_A
Cuts at: 0 1383 3615
Size: 1383 2232
BfrBI ATG'CAT
Cuts at: 0 3340 3615
Size: 3340 275
BglII A'GATC_T
Cuts at: 0 1021 2334 3241 3615
Size: 1021 1313 907 374
Fragments arranged by size:
1313 1021 907 374
BlpI GC'TnA_GC
Cuts at: 0 3401 3615
Size: 3401 214
Bme1580I G_kGCm'C
Cuts at: 0 1480 2837 2894 3615
Size: 1480 1357 57 721
Fragments arranged by size:
1480 1357 721 57
BmgBI CAC'GTC
Cuts at: 0 216 828 3615
Size: 216 612 2787
Fragments arranged by size:
2787 612 216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2579 3416 3615
Size: 2579 837 199
Fragments arranged by size:
2579 837 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1094 1982 2943 3615
Size: 122 972 888 961 672
Fragments arranged by size:
972 961 888 672 122
Bpu10I CC'TnA_GC
Cuts at: 0 230 3615
Size: 230 3385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2417 3615
Size: 2417 1198
BsaI GGTCTCn'nnnn_
Cuts at: 0 2567 3277 3615
Size: 2567 710 338
Fragments arranged by size:
2567 710 338
BsaAI yAC'GTr
Cuts at: 0 398 3615
Size: 398 3217
BsaBI GATnn'nnATC
Cuts at: 0 272 3615
Size: 272 3343
BsaHI Gr'CG_yC
Cuts at: 0 738 1006 3585 3615
Size: 738 268 2579 30
Fragments arranged by size:
2579 738 268 30
BsaWI w'CCGG_w
Cuts at: 0 407 3615
Size: 407 3208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 2036 2066 2148 2178 2916 2946
Size: 979 30 1027 30 82 30 738 30
Cuts at: 2946 3615
Size: 669
Fragments arranged by size:
1027 979 738 669 82 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2057 2132 2237 3408 3615
Size: 2057 75 105 1171 207
Fragments arranged by size:
2057 1171 207 105 75
BseYI C'CCAG_C
Cuts at: 0 783 930 1001 2983 3615
Size: 783 147 71 1982 632
Fragments arranged by size:
1982 783 632 147 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2733 2885 3615
Size: 2733 152 730
Fragments arranged by size:
2733 730 152
BsiHKAI G_wGCw'C
Cuts at: 0 827 1480 1842 2837 3402 3615
Size: 827 653 362 995 565 213
Fragments arranged by size:
995 827 653 565 362 213
BsmI GAATG_Cn'
Cuts at: 0 1100 1294 1635 1816 3615
Size: 1100 194 341 181 1799
Fragments arranged by size:
1799 1100 341 194 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 729 3615
Size: 729 2886
Bsp1286I G_dGCh'C
Cuts at: 0 827 1480 1842 2837 2894 3402 3615
Size: 827 653 362 995 57 508 213
Fragments arranged by size:
995 827 653 508 362 213 57
BspEI T'CCGG_A
Cuts at: 0 407 3615
Size: 407 3208
BspHI T'CATG_A
Cuts at: 0 1155 1386 3204 3615
Size: 1155 231 1818 411
Fragments arranged by size:
1818 1155 411 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1095 2950 2958 3615
Size: 1095 1855 8 657
Fragments arranged by size:
1855 1095 657 8
BsrBI CCG'CTC
Cuts at: 0 34 2450 2828 3615
Size: 34 2416 378 787
Fragments arranged by size:
2416 787 378 34
BsrDI GCAATG_nn'
Cuts at: 0 1447 1614 3615
Size: 1447 167 2001
Fragments arranged by size:
2001 1447 167
BsrFI r'CCGG_y
Cuts at: 0 1117 2873 3615
Size: 1117 1756 742
Fragments arranged by size:
1756 1117 742
BsrGI T'GTAC_A
Cuts at: 0 2710 3615
Size: 2710 905
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2846 3346 3615
Size: 2846 500 269
Fragments arranged by size:
2846 500 269
BstEII G'GTnAC_C
Cuts at: 0 2854 3432 3615
Size: 2854 578 183
Fragments arranged by size:
2854 578 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 984 2998 3615
Size: 984 2014 617
Fragments arranged by size:
2014 984 617
BstYI r'GATC_y
Cuts at: 0 1021 2019 2334 3241 3615
Size: 1021 998 315 907 374
Fragments arranged by size:
1021 998 907 374 315
BtgI C'CryG_G
Cuts at: 0 813 3615
Size: 813 2802
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2629 3615
Size: 2629 986
BtsI GCAGTG_nn'
Cuts at: 0 1329 1852 2130 2745 3615
Size: 1329 523 278 615 870
Fragments arranged by size:
1329 870 615 523 278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 2562 2597 2742 2777 3615
Size: 1224 35 1303 35 145 35 838
Fragments arranged by size:
1303 1224 838 145 35 35 35
DraI TTT'AAA
Cuts at: 0 2294 3615
Size: 2294 1321
DrdI GACnn_nn'nnGTC
Cuts at: 0 802 3615
Size: 802 2813
EarI CTCTTCn'nnn_
Cuts at: 0 524 2548 3324 3615
Size: 524 2024 776 291
Fragments arranged by size:
2024 776 524 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 122 1094 1818 1982 2785 2943 3305
Size: 38 84 972 724 164 803 158 362
Cuts at: 3305 3615
Size: 310
Fragments arranged by size:
972 803 724 362 310 164 158 84
38
EcoO109I rG'GnC_Cy
Cuts at: 0 1561 2917 2970 3615
Size: 1561 1356 53 645
Fragments arranged by size:
1561 1356 645 53
FspI TGC'GCA
Cuts at: 0 919 3615
Size: 919 2696
FspAI rTGC'GCAy
Cuts at: 0 919 3615
Size: 919 2696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 1013 1045 2415 2434 2447 2466
Size: 152 32 829 32 1370 19 13 19
Cuts at: 2466 3202 3234 3615
Size: 736 32 381
Fragments arranged by size:
1370 829 736 381 152 32 32 32
19 19 13
HincII GTy'rAC
Cuts at: 0 3031 3615
Size: 3031 584
HindIII A'AGCT_T
Cuts at: 0 981 2173 3615
Size: 981 1192 1442
Fragments arranged by size:
1442 1192 981
KpnI G_GTAC'C
Cuts at: 0 2184 3615
Size: 2184 1431
MfeI C'AATT_G
Cuts at: 0 1656 3615
Size: 1656 1959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 644 3613 3615
Size: 248 396 2969 2
Fragments arranged by size:
2969 396 248 2
MslI CAynn'nnrTG
Cuts at: 0 634 902 925 1052 1120 1160 1355 1728
Size: 634 268 23 127 68 40 195 373
Cuts at: 1728 1845 2035 2390 2996 3209 3615
Size: 117 190 355 606 213 406
Fragments arranged by size:
634 606 406 373 355 268 213 195
190 127 117 68 40 23
MspA1I CmG'CkG
Cuts at: 0 58 613 720 1512 3615
Size: 58 555 107 792 2103
Fragments arranged by size:
2103 792 555 107 58
NaeI GCC'GGC
Cuts at: 0 2875 3615
Size: 2875 740
NcoI C'CATG_G
Cuts at: 0 813 3615
Size: 813 2802
NdeI CA'TA_TG
Cuts at: 0 1905 2032 3342 3615
Size: 1905 127 1310 273
Fragments arranged by size:
1905 1310 273 127
NgoMIV G'CCGG_C
Cuts at: 0 2873 3615
Size: 2873 742
NsiI A_TGCA'T
Cuts at: 0 3342 3615
Size: 3342 273
NspI r_CATG'y
Cuts at: 0 639 1125 1623 1700 2221 3615
Size: 639 486 498 77 521 1394
Fragments arranged by size:
1394 639 521 498 486 77
PasI CC'CwG_GG
Cuts at: 0 2206 3615
Size: 2206 1409
PciI A'CATG_T
Cuts at: 0 635 1696 2217 3615
Size: 635 1061 521 1398
Fragments arranged by size:
1398 1061 635 521
PflMI CCAn_nnn'nTGG
Cuts at: 0 1209 1573 3615
Size: 1209 364 2042
Fragments arranged by size:
2042 1209 364
PfoI T'CCnGG_A
Cuts at: 0 465 1413 2519 3615
Size: 465 948 1106 1096
Fragments arranged by size:
1106 1096 948 465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 2916 2948 3615
Size: 223 32 2661 32 667
Fragments arranged by size:
2661 667 223 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1561 2917 2970 3615
Size: 1561 1356 53 645
Fragments arranged by size:
1561 1356 645 53
PshAI GACnn'nnGTC
Cuts at: 0 139 3615
Size: 139 3476
PsiI TTA'TAA
Cuts at: 0 2357 3125 3615
Size: 2357 768 490
Fragments arranged by size:
2357 768 490
PstI C_TGCA'G
Cuts at: 0 2980 3151 3615
Size: 2980 171 464
Fragments arranged by size:
2980 464 171
PvuII CAG'CTG
Cuts at: 0 58 613 720 1512 3615
Size: 58 555 107 792 2103
Fragments arranged by size:
2103 792 555 107 58
SapI GCTCTTCn'nnn_
Cuts at: 0 2548 3615
Size: 2548 1067
SexAI A'CCwGG_T
Cuts at: 0 3117 3615
Size: 3117 498
SfcI C'TryA_G
Cuts at: 0 614 654 2276 2976 3147 3615
Size: 614 40 1622 700 171 468
Fragments arranged by size:
1622 700 614 468 171 40
SmlI C'TyrA_G
Cuts at: 0 2185 2432 3615
Size: 2185 247 1183
Fragments arranged by size:
2185 1183 247
SnaBI TAC'GTA
Cuts at: 0 398 3615
Size: 398 3217
SpeI A'CTAG_T
Cuts at: 0 300 3615
Size: 300 3315
SspI AAT'ATT
Cuts at: 0 2700 3615
Size: 2700 915
StuI AGG'CCT
Cuts at: 0 677 3615
Size: 677 2938
StyI C'CwwG_G
Cuts at: 0 548 813 1280 1952 3615
Size: 548 265 467 672 1663
Fragments arranged by size:
1663 672 548 467 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2842 3006 3468 3615
Size: 2842 164 462 147
Fragments arranged by size:
2842 462 164 147
TatI w'GTAC_w
Cuts at: 0 2468 2597 2710 3615
Size: 2468 129 113 905
Fragments arranged by size:
2468 905 129 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1569 1601 2036 2068 2945 2977
Size: 979 32 558 32 435 32 877 32
Cuts at: 2977 3615
Size: 638
Fragments arranged by size:
979 877 638 558 435 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 153 3186 3615
Size: 153 3033 429
Fragments arranged by size:
3033 429 153
XmnI GAAnn'nnTTC
Cuts at: 0 2381 3615
Size: 2381 1234
ZraI GAC'GTC
Cuts at: 0 3586 3615
Size: 3586 29
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AvaI BanI BbsI BclI
BfrBI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EarI
Eco57MI EcoO109I FspI FspAI Hin4I HincII HindIII KpnI
MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PsiI PstI PvuII SapI SexAI SfcI SmlI
SnaBI SpeI SspI StuI StyI TaqII TatI TstI
XbaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AhdI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BglI BmrI BmtI BsiEI BsiWI BssHII BssSI
BstBI BstZ17I Bsu36I ClaI DraIII EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI HaeII HpaI
KasI MluI MscI NarI NheI NotI NruI PacI
PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SphI SrfI SwaI Tth111I XcmI XhoI
XmaI