Restriction map for
pF1KA0336
(Linear) (Six-base) MAPSORT of: KIAA0336.seq Check: 371 from: 1 to: 4749
KIAA0336.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 1406 2993 4749
Size: 1406 1587 1756
Fragments arranged by size:
1756 1587 1406
Acc65I G'GTAC_C
Cuts at: 0 1356 4749
Size: 1356 3393
AclI AA'CG_TT
Cuts at: 0 3195 4749
Size: 3195 1554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1748 1772 1873 2513 3004 3061 3094
Size: 245 1503 24 101 640 491 57 33
Cuts at: 3094 3308 4667 4749
Size: 214 1359 82
Fragments arranged by size:
1503 1359 640 491 245 214 101 82
57 33 24
AflII C'TTAA_G
Cuts at: 0 778 982 1081 2182 2223 4120 4749
Size: 778 204 99 1101 41 1897 629
Fragments arranged by size:
1897 1101 778 629 204 99 41
AflIII A'CryG_T
Cuts at: 0 2359 4749
Size: 2359 2390
AhdI GACnn_n'nnGTC
Cuts at: 0 3983 4749
Size: 3983 766
AlwNI CAG_nnn'CTG
Cuts at: 0 3415 4749
Size: 3415 1334
ApoI r'AATT_y
Cuts at: 0 93 208 287 597 1160 1464 1509 2862
Size: 93 115 79 310 563 304 45 1353
Cuts at: 2862 2889 3269 3652 4360 4749
Size: 27 380 383 708 389
Fragments arranged by size:
1353 708 563 389 383 380 310 304
115 93 79 45 27
AseI AT'TA_AT
Cuts at: 0 398 857 1060 3818 4749
Size: 398 459 203 2758 931
Fragments arranged by size:
2758 931 459 398 203
AvaI C'yCGr_G
Cuts at: 0 4229 4749
Size: 4229 520
BanI G'GyrC_C
Cuts at: 0 1356 4749
Size: 1356 3393
BbsI GAAGACnn'nnnn_
Cuts at: 0 143 845 1736 2018 2519 2846 3314 4268
Size: 143 702 891 282 501 327 468 954
Cuts at: 4268 4749
Size: 481
Fragments arranged by size:
954 891 702 501 481 468 327 282
143
BclI T'GATC_A
Cuts at: 0 3417 4749
Size: 3417 1332
BglI GCCn_nnn'nGGC
Cuts at: 0 2623 4749
Size: 2623 2126
BglII A'GATC_T
Cuts at: 0 2727 2904 4749
Size: 2727 177 1845
Fragments arranged by size:
2727 1845 177
BlpI GC'TnA_GC
Cuts at: 0 4639 4749
Size: 4639 110
BmrI ACTGGGnnnn_n'
Cuts at: 0 1186 4141 4749
Size: 1186 2955 608
Fragments arranged by size:
2955 1186 608
BmtI G_CTAG'C
Cuts at: 0 922 4749
Size: 922 3827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 3475 4749
Size: 1300 2175 1274
Fragments arranged by size:
2175 1300 1274
Bpu10I CC'TnA_GC
Cuts at: 0 485 2490 4749
Size: 485 2005 2259
Fragments arranged by size:
2259 2005 485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4357 4661 4749
Size: 4357 304 88
Fragments arranged by size:
4357 304 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 2650 4749
Size: 2650 2099
BsaBI GATnn'nnATC
Cuts at: 0 4716 4749
Size: 4716 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4258 4288 4749
Size: 4258 30 461
Fragments arranged by size:
4258 461 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 1669 2602 4749
Size: 295 1374 933 2147
Fragments arranged by size:
2147 1374 933 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4430 4749
Size: 4430 319
BsiHKAI G_wGCw'C
Cuts at: 0 3536 4749
Size: 3536 1213
BsiWI C'GTAC_G
Cuts at: 0 3565 4749
Size: 3565 1184
BsmI GAATG_Cn'
Cuts at: 0 2298 2578 4749
Size: 2298 280 2171
Fragments arranged by size:
2298 2171 280
Bsp1286I G_dGCh'C
Cuts at: 0 3536 4749
Size: 3536 1213
BspHI T'CATG_A
Cuts at: 0 828 1245 4749
Size: 828 417 3504
Fragments arranged by size:
3504 828 417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 913 4749
Size: 913 3836
BsrDI GCAATG_nn'
Cuts at: 0 179 2993 3838 4749
Size: 179 2814 845 911
Fragments arranged by size:
2814 911 845 179
BssSI C'ACGA_G
Cuts at: 0 3529 4749
Size: 3529 1220
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4453 4749
Size: 4453 296
BstBI TT'CG_AA
Cuts at: 0 1211 4014 4749
Size: 1211 2803 735
Fragments arranged by size:
2803 1211 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2988 4749
Size: 2988 1761
BstYI r'GATC_y
Cuts at: 0 2727 2904 4749
Size: 2727 177 1845
Fragments arranged by size:
2727 1845 177
BstZ17I GTA'TAC
Cuts at: 0 1407 4749
Size: 1407 3342
BtsI GCAGTG_nn'
Cuts at: 0 2881 3859 4749
Size: 2881 978 890
Fragments arranged by size:
2881 978 890
DraI TTT'AAA
Cuts at: 0 852 929 1130 1664 1919 3443 4749
Size: 852 77 201 534 255 1524 1306
Fragments arranged by size:
1524 1306 852 534 255 201 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 1029 2931 4749
Size: 1029 1902 1818
Fragments arranged by size:
1902 1818 1029
EarI CTCTTCn'nnn_
Cuts at: 0 245 407 440 1898 2015 2150 2693 3602
Size: 245 162 33 1458 117 135 543 909
Cuts at: 3602 4117 4229 4749
Size: 515 112 520
Fragments arranged by size:
1458 909 543 520 515 245 162 135
117 112 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3925 4269 4749
Size: 3925 344 480
Fragments arranged by size:
3925 480 344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1300 1748 1772 1873 2513 3004 3061
Size: 245 1055 448 24 101 640 491 57
Cuts at: 3061 3094 3308 3475 4667 4749
Size: 33 214 167 1192 82
Fragments arranged by size:
1192 1055 640 491 448 245 214 167
101 82 57 33 24
EcoO109I rG'GnC_Cy
Cuts at: 0 2262 4008 4749
Size: 2262 1746 741
Fragments arranged by size:
2262 1746 741
EcoRI G'AATT_C
Cuts at: 0 3652 4749
Size: 3652 1097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1309 2505 4578 4749
Size: 1309 1196 2073 171
Fragments arranged by size:
2073 1309 1196 171
HaeII r_GCGC'y
Cuts at: 0 3737 4749
Size: 3737 1012
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1198 1230 1452 1484 2509 2541 2695 2727
Size: 1198 32 222 32 1025 32 154 32
Cuts at: 2727 3969 4001 4252 4258 4284 4290 4749
Size: 1242 32 251 6 26 6 459
Fragments arranged by size:
1242 1198 1025 459 251 222 154 32
32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 0 3224 4749
Size: 3224 1525
HindIII A'AGCT_T
Cuts at: 0 4477 4749
Size: 4477 272
HpaI GTT'AAC
Cuts at: 0 3224 4749
Size: 3224 1525
KpnI G_GTAC'C
Cuts at: 0 1360 4749
Size: 1360 3389
MfeI C'AATT_G
Cuts at: 0 3364 4749
Size: 3364 1385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 2522 4749
Size: 35 2487 2227
Fragments arranged by size:
2487 2227 35
MslI CAynn'nnrTG
Cuts at: 0 3166 3955 4749
Size: 3166 789 794
Fragments arranged by size:
3166 794 789
MspA1I CmG'CkG
Cuts at: 0 162 1750 3495 4311 4749
Size: 162 1588 1745 816 438
Fragments arranged by size:
1745 1588 816 438 162
NheI G'CTAG_C
Cuts at: 0 918 4749
Size: 918 3831
NspI r_CATG'y
Cuts at: 0 2363 3049 4218 4357 4749
Size: 2363 686 1169 139 392
Fragments arranged by size:
2363 1169 686 392 139
PciI A'CATG_T
Cuts at: 0 2359 4749
Size: 2359 2390
PfoI T'CCnGG_A
Cuts at: 0 3917 4749
Size: 3917 832
PpuMI rG'GwC_Cy
Cuts at: 0 4008 4749
Size: 4008 741
PsiI TTA'TAA
Cuts at: 0 1495 2404 4621 4749
Size: 1495 909 2217 128
Fragments arranged by size:
2217 1495 909 128
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1260 1292 3065 3097 4749
Size: 1260 32 1773 32 1652
Fragments arranged by size:
1773 1652 1260 32 32
PstI C_TGCA'G
Cuts at: 0 1256 2270 3890 4536 4571 4749
Size: 1256 1014 1620 646 35 178
Fragments arranged by size:
1620 1256 1014 646 178 35
PvuII CAG'CTG
Cuts at: 0 162 1750 3495 4311 4749
Size: 162 1588 1745 816 438
Fragments arranged by size:
1745 1588 816 438 162
SapI GCTCTTCn'nnn_
Cuts at: 0 407 1898 4117 4749
Size: 407 1491 2219 632
Fragments arranged by size:
2219 1491 632 407
SexAI A'CCwGG_T
Cuts at: 0 4587 4749
Size: 4587 162
SfcI C'TryA_G
Cuts at: 0 950 1102 1252 2044 2266 3601 3886 4532
Size: 950 152 150 792 222 1335 285 646
Cuts at: 4532 4567 4749
Size: 35 182
Fragments arranged by size:
1335 950 792 646 285 222 182 152
150 35
SmaI CCC'GGG
Cuts at: 0 4231 4749
Size: 4231 518
SmlI C'TyrA_G
Cuts at: 0 778 982 1081 2182 2223 4120 4336 4676
Size: 778 204 99 1101 41 1897 216 340
Cuts at: 4676 4749
Size: 73
Fragments arranged by size:
1897 1101 778 340 216 204 99 73
41
SphI G_CATG'C
Cuts at: 0 4357 4749
Size: 4357 392
SspI AAT'ATT
Cuts at: 0 396 4749
Size: 396 4353
StyI C'CwwG_G
Cuts at: 0 2063 3623 4749
Size: 2063 1560 1126
Fragments arranged by size:
2063 1560 1126
SwaI ATTT'AAAT
Cuts at: 0 1919 4749
Size: 1919 2830
TatI w'GTAC_w
Cuts at: 0 972 1604 2955 3327 4749
Size: 972 632 1351 372 1422
Fragments arranged by size:
1422 1351 972 632 372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3913 3945 4097 4129 4749
Size: 3913 32 152 32 620
Fragments arranged by size:
3913 620 152 32 32
XbaI T'CTAG_A
Cuts at: 0 2280 4209 4749
Size: 2280 1929 540
Fragments arranged by size:
2280 1929 540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3387 4374 4749
Size: 3387 987 375
Fragments arranged by size:
3387 987 375
XmaI C'CCGG_G
Cuts at: 0 4229 4749
Size: 4229 520
XmnI GAAnn'nnTTC
Cuts at: 0 2687 3017 3074 4379 4749
Size: 2687 330 57 1305 370
Fragments arranged by size:
2687 1305 370 330 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlwNI
ApoI AseI AvaI BanI BbsI BclI BglI BglII
BlpI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI
BsaXI BseRI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspHI
BspMI BsrDI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtsI DraI DrdI EarI EciI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MmeI MslI MspA1I NheI NspI PciI PfoI PpuMI
PsiI PsrI PstI PvuII SapI SexAI SfcI SmaI
SmlI SphI SspI StyI SwaI TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BciVI BfrBI Bme1580I BmgBI BplI BsaAI
BsaHI BsaWI BseYI BsiEI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BstEII Bsu36I BtgI BtgZI ClaI CspCI
DraIII EaeI EagI EcoICRI EcoNI EcoRV FseI FspI
FspAI KasI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI StuI TaqII Tth111I XhoI
ZraI