Restriction map for
pF1KA0330
(Linear) (Six-base) MAPSORT of: KIAA0330.seq Check: 2711 from: 1 to: 6660
KIAA0330.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2898 5670 5941 6099 6202 6660
Size: 2898 2772 271 158 103 458
Fragments arranged by size:
2898 2772 458 271 158 103
AccI GT'mk_AC
Cuts at: 0 1223 1541 6660
Size: 1223 318 5119
Fragments arranged by size:
5119 1223 318
Acc65I G'GTAC_C
Cuts at: 0 2882 3117 6660
Size: 2882 235 3543
Fragments arranged by size:
3543 2882 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 2071 2269 2440 2513 2807 3046 3355
Size: 1879 192 198 171 73 294 239 309
Cuts at: 3355 3835 3914 3959 4261 4870 4888 6026 6535
Size: 480 79 45 302 609 18 1138 509
Cuts at: 6535 6660
Size: 125
Fragments arranged by size:
1879 1138 609 509 480 309 302 294
239 198 192 171 125 79 73 45
18
AfeI AGC'GCT
Cuts at: 0 746 6660
Size: 746 5914
AflIII A'CryG_T
Cuts at: 0 393 526 659 4652 6660
Size: 393 133 133 3993 2008
Fragments arranged by size:
3993 2008 393 133 133
AgeI A'CCGG_T
Cuts at: 0 149 6660
Size: 149 6511
AhdI GACnn_n'nnGTC
Cuts at: 0 5559 6660
Size: 5559 1101
AleI CACnn'nnGTG
Cuts at: 0 1030 3043 6660
Size: 1030 2013 3617
Fragments arranged by size:
3617 2013 1030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1588 1945 2512 3603 6660
Size: 1588 357 567 1091 3057
Fragments arranged by size:
3057 1588 1091 567 357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2920 2952 6660
Size: 2920 32 3708
Fragments arranged by size:
3708 2920 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1668 1902 1969 2076 2094 4387 4488 4941
Size: 1668 234 67 107 18 2293 101 453
Cuts at: 4941 5842 6166 6184 6273 6513 6660
Size: 901 324 18 89 240 147
Fragments arranged by size:
2293 1668 901 453 324 240 234 147
107 101 89 67 18 18
ApaI G_GGCC'C
Cuts at: 0 3350 3396 3997 4647 5027 5156 5270 5368
Size: 3350 46 601 650 380 129 114 98
Cuts at: 5368 6037 6660
Size: 669 623
Fragments arranged by size:
3350 669 650 623 601 380 129 114
98 46
ApaLI G'TGCA_C
Cuts at: 0 2148 6660
Size: 2148 4512
ApoI r'AATT_y
Cuts at: 0 2974 6660
Size: 2974 3686
AvaI C'yCGr_G
Cuts at: 0 3293 5155 5368 5760 6660
Size: 3293 1862 213 392 900
Fragments arranged by size:
3293 1862 900 392 213
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3101 3134 6660
Size: 3101 33 3526
Fragments arranged by size:
3526 3101 33
BamHI G'GATC_C
Cuts at: 0 416 4757 6660
Size: 416 4341 1903
Fragments arranged by size:
4341 1903 416
BanI G'GyrC_C
Cuts at: 0 2066 2882 3117 3439 5829 6070 6660
Size: 2066 816 235 322 2390 241 590
Fragments arranged by size:
2390 2066 816 590 322 241 235
BanII G_rGCy'C
Cuts at: 0 185 866 2519 2733 3350 3396 3515 3997
Size: 185 681 1653 214 617 46 119 482
Cuts at: 3997 4187 4647 4712 5000 5027 5156 5257 5270
Size: 190 460 65 288 27 129 101 13
Cuts at: 5270 5279 5368 6037 6191 6280 6362 6660
Size: 9 89 669 154 89 82 298
Fragments arranged by size:
1653 681 669 617 482 460 298 288
214 190 185 154 129 119 101 89
89 82 65 46 27 13 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 2582 2795 4988 6660
Size: 2582 213 2193 1672
Fragments arranged by size:
2582 2193 1672 213
BbvCI CC'TCA_GC
Cuts at: 0 1588 4410 4502 6660
Size: 1588 2822 92 2158
Fragments arranged by size:
2822 2158 1588 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 183 217 3703 3737 6660
Size: 183 34 3486 34 2923
Fragments arranged by size:
3486 2923 183 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4681 6660
Size: 4681 1979
BclI T'GATC_A
Cuts at: 0 45 6660
Size: 45 6615
BfrBI ATG'CAT
Cuts at: 0 3237 6660
Size: 3237 3423
BglI GCCn_nnn'nGGC
Cuts at: 0 295 1362 3775 5102 5991 6000 6660
Size: 295 1067 2413 1327 889 9 660
Fragments arranged by size:
2413 1327 1067 889 660 295 9
BglII A'GATC_T
Cuts at: 0 4272 6660
Size: 4272 2388
BlpI GC'TnA_GC
Cuts at: 0 1534 4990 5404 5527 6185 6660
Size: 1534 3456 414 123 658 475
Fragments arranged by size:
3456 1534 658 475 414 123
Bme1580I G_kGCm'C
Cuts at: 0 2152 3350 3396 3997 4647 5027 5156 5270
Size: 2152 1198 46 601 650 380 129 114
Cuts at: 5270 5368 5418 5834 6037 6073 6178 6327 6660
Size: 98 50 416 203 36 105 149 333
Fragments arranged by size:
2152 1198 650 601 416 380 333 203
149 129 114 105 98 50 46 36
BmgBI CAC'GTC
Cuts at: 0 871 1410 2523 3475 4653 6660
Size: 871 539 1113 952 1178 2007
Fragments arranged by size:
2007 1178 1113 952 871 539
BmrI ACTGGGnnnn_n'
Cuts at: 0 5870 6531 6660
Size: 5870 661 129
Fragments arranged by size:
5870 661 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2913 4024 4471 5535 6281 6660
Size: 2913 1111 447 1064 746 379
Fragments arranged by size:
2913 1111 1064 746 447 379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1447 1474 1496 1572 1636 1852 1918
Size: 337 1110 27 22 76 64 216 66
Cuts at: 1918 1924 2050 2080 2098 2200 2743 2836 2908
Size: 6 126 30 18 102 543 93 72
Cuts at: 2908 3137 3418 3802 4191 4621 5416 5569 5722
Size: 229 281 384 389 430 795 153 153
Cuts at: 5722 6604 6613 6640 6660
Size: 882 9 27 20
Fragments arranged by size:
1110 882 795 543 430 389 384 337
281 229 216 153 153 126 102 93
76 72 66 64 30 27 27 22
20 18 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 92 1588 3070 3509 3761 4410 4502 4558
Size: 92 1496 1482 439 252 649 92 56
Cuts at: 4558 5142 6221 6245 6274 6356 6660
Size: 584 1079 24 29 82 304
Fragments arranged by size:
1496 1482 1079 649 584 439 304 252
92 92 82 56 29 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 949 4813 6660
Size: 574 375 3864 1847
Fragments arranged by size:
3864 1847 574 375
BsaI GGTCTCn'nnnn_
Cuts at: 0 1023 5653 6493 6660
Size: 1023 4630 840 167
Fragments arranged by size:
4630 1023 840 167
BsaBI GATnn'nnATC
Cuts at: 0 2377 6660
Size: 2377 4283
BsaHI Gr'CG_yC
Cuts at: 0 949 2644 6660
Size: 949 1695 4016
Fragments arranged by size:
4016 1695 949
BsaWI w'CCGG_w
Cuts at: 0 149 220 413 623 2555 4628 6660
Size: 149 71 193 210 1932 2073 2032
Fragments arranged by size:
2073 2032 1932 210 193 149 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1068 1098 3110 3140 3949 3979 4041 4071
Size: 1068 30 2012 30 809 30 62 30
Cuts at: 4071 5156 5186 5922 5952 6660
Size: 1085 30 736 30 708
Fragments arranged by size:
2012 1085 1068 809 736 708 62 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 622 1297 1569 1691 2047 2095 2356
Size: 172 450 675 272 122 356 48 261
Cuts at: 2356 2518 2824 3622 3625 3730 3922 4005 4008
Size: 162 306 798 3 105 192 83 3
Cuts at: 4008 4324 4519 5170 5413 5524 5704 6511 6660
Size: 316 195 651 243 111 180 807 149
Fragments arranged by size:
807 798 675 651 450 356 316 306
272 261 243 195 192 180 172 162
149 122 111 105 83 48 3 3
BseYI C'CCAG_C
Cuts at: 0 290 302 874 916 990 1802 2213 2619
Size: 290 12 572 42 74 812 411 406
Cuts at: 2619 2860 2903 3633 3979 4380 4571 4841 5111
Size: 241 43 730 346 401 191 270 270
Cuts at: 5111 5442 5447 6016 6310 6326 6361 6660
Size: 331 5 569 294 16 35 299
Fragments arranged by size:
812 730 572 569 411 406 401 346
331 299 294 290 270 270 241 191
74 43 42 35 16 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 805 1039 1905 2518 3538 3667 3694
Size: 707 98 234 866 613 1020 129 27
Cuts at: 3694 3869 5240 5888 6660
Size: 175 1371 648 772
Fragments arranged by size:
1371 1020 866 772 707 648 613 234
175 129 98 27
BsiEI CG_ry'CG
Cuts at: 0 4119 4783 6660
Size: 4119 664 1877
Fragments arranged by size:
4119 1877 664
BsiHKAI G_wGCw'C
Cuts at: 0 185 1535 2152 3515 4979 5000 6280 6660
Size: 185 1350 617 1363 1464 21 1280 380
Fragments arranged by size:
1464 1363 1350 1280 617 380 185 21
BsmI GAATG_Cn'
Cuts at: 0 3305 6660
Size: 3305 3355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2529 2635 6660
Size: 2529 106 4025
Fragments arranged by size:
4025 2529 106
Bsp1286I G_dGCh'C
Cuts at: 0 185 866 1535 2152 2519 2733 3350 3396
Size: 185 681 669 617 367 214 617 46
Cuts at: 3396 3515 3997 4187 4647 4712 4979 5000 5027
Size: 119 482 190 460 65 267 21 27
Cuts at: 5027 5156 5257 5270 5279 5368 5418 5834 6037
Size: 129 101 13 9 89 50 416 203
Cuts at: 6037 6073 6178 6191 6280 6327 6362 6660
Size: 36 105 13 89 47 35 298
Fragments arranged by size:
681 669 617 617 482 460 416 367
298 267 214 203 190 185 129 119
105 101 89 89 65 50 47 46
36 35 27 21 13 13 9
BspEI T'CCGG_A
Cuts at: 0 413 623 6660
Size: 413 210 6037
Fragments arranged by size:
6037 413 210
BspHI T'CATG_A
Cuts at: 0 48 6660
Size: 48 6612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2898 3716 5670 5941 6099 6202 6660
Size: 2898 818 1954 271 158 103 458
Fragments arranged by size:
2898 1954 818 458 271 158 103
BsrBI CCG'CTC
Cuts at: 0 2064 4811 5238 5322 5436 5517 6660
Size: 2064 2747 427 84 114 81 1143
Fragments arranged by size:
2747 2064 1143 427 114 84 81
BsrFI r'CCGG_y
Cuts at: 0 149 566 3533 4491 4698 4778 5189 5375
Size: 149 417 2967 958 207 80 411 186
Cuts at: 5375 6156 6660
Size: 781 504
Fragments arranged by size:
2967 958 781 504 417 411 207 186
149 80
BsrGI T'GTAC_A
Cuts at: 0 503 839 2849 6660
Size: 503 336 2010 3811
Fragments arranged by size:
3811 2010 503 336
BssSI C'ACGA_G
Cuts at: 0 2686 3514 3712 5286 6660
Size: 2686 828 198 1574 1374
Fragments arranged by size:
2686 1574 1374 828 198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1410 4686 4695 5911 6166 6660
Size: 1410 3276 9 1216 255 494
Fragments arranged by size:
3276 1410 1216 494 255 9
BstBI TT'CG_AA
Cuts at: 0 6 6660
Size: 6 6654
BstEII G'GTnAC_C
Cuts at: 0 1771 6340 6660
Size: 1771 4569 320
Fragments arranged by size:
4569 1771 320
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1389 1956 2178 2886 4197 6152 6660
Size: 1389 567 222 708 1311 1955 508
Fragments arranged by size:
1955 1389 1311 708 567 508 222
BstYI r'GATC_y
Cuts at: 0 312 416 2913 3740 4272 4757 6660
Size: 312 104 2497 827 532 485 1903
Fragments arranged by size:
2497 1903 827 532 485 312 104
Bsu36I CC'TnA_GG
Cuts at: 0 403 1280 5087 6086 6660
Size: 403 877 3807 999 574
Fragments arranged by size:
3807 999 877 574 403
BtgI C'CryG_G
Cuts at: 0 326 938 1481 3770 4675 4763 5201 5257
Size: 326 612 543 2289 905 88 438 56
Cuts at: 5257 5279 6094 6660
Size: 22 815 566
Fragments arranged by size:
2289 905 815 612 566 543 438 326
88 56 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4521 6660
Size: 4521 2139
BtsI GCAGTG_nn'
Cuts at: 0 353 1051 1700 1761 2269 2412 2839 3493
Size: 353 698 649 61 508 143 427 654
Cuts at: 3493 3985 4537 6147 6321 6660
Size: 492 552 1610 174 339
Fragments arranged by size:
1610 698 654 649 552 508 492 427
353 339 174 143 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2427 2462 4044 4079 5016 5051 6660
Size: 2427 35 1582 35 937 35 1609
Fragments arranged by size:
2427 1609 1582 937 35 35 35
DraI TTT'AAA
Cuts at: 0 63 6660
Size: 63 6597
DraIII CAC_nnn'GTG
Cuts at: 0 1202 2147 4430 6660
Size: 1202 945 2283 2230
Fragments arranged by size:
2283 2230 1202 945
DrdI GACnn_nn'nnGTC
Cuts at: 0 4936 6660
Size: 4936 1724
EaeI y'GGCC_r
Cuts at: 0 833 2351 3014 3699 3767 4780 5094 5198
Size: 833 1518 663 685 68 1013 314 104
Cuts at: 5198 5992 6154 6660
Size: 794 162 506
Fragments arranged by size:
1518 1013 833 794 685 663 506 314
162 104 68
EagI C'GGCC_G
Cuts at: 0 4780 6660
Size: 4780 1880
EarI CTCTTCn'nnn_
Cuts at: 0 1145 1208 1971 2532 2863 3022 3331 3978
Size: 1145 63 763 561 331 159 309 647
Cuts at: 3978 4222 4438 4717 6041 6660
Size: 244 216 279 1324 619
Fragments arranged by size:
1324 1145 763 647 619 561 331 309
279 244 216 159 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1537 2125 4537 4770 6405 6660
Size: 1537 588 2412 233 1635 255
Fragments arranged by size:
2412 1635 1537 588 255 233
EcoICRI GAG'CTC
Cuts at: 0 183 3513 4998 6278 6660
Size: 183 3330 1485 1280 382
Fragments arranged by size:
3330 1485 1280 382 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1447 1474 1496 1572 1636 1852 1879
Size: 337 1110 27 22 76 64 216 27
Cuts at: 1879 1918 1924 2050 2071 2080 2098 2200 2269
Size: 39 6 126 21 9 18 102 69
Cuts at: 2269 2440 2513 2743 2807 2836 2908 3046 3137
Size: 171 73 230 64 29 72 138 91
Cuts at: 3137 3355 3418 3802 3835 3914 3959 4191 4261
Size: 218 63 384 33 79 45 232 70
Cuts at: 4261 4621 4870 4888 5416 5569 5722 6026 6535
Size: 360 249 18 528 153 153 304 509
Cuts at: 6535 6604 6613 6640 6660
Size: 69 9 27 20
Fragments arranged by size:
1110 528 509 384 360 337 304 249
232 230 218 216 171 153 153 138
126 102 91 79 76 73 72 70
69 69 64 64 63 45 39 33
29 27 27 27 22 21 20 18
18 9 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1816 2470 3130 3712 3897 4867 6660
Size: 1816 654 660 582 185 970 1793
Fragments arranged by size:
1816 1793 970 660 654 582 185
EcoO109I rG'GnC_Cy
Cuts at: 0 126 912 1757 1899 2388 2705 3393 3777
Size: 126 786 845 142 489 317 688 384
Cuts at: 3777 3855 3889 3994 4452 4938 5023 5103 5152
Size: 78 34 105 458 486 85 80 49
Cuts at: 5152 5266 5364 5365 5976 6306 6588 6660
Size: 114 98 1 611 330 282 72
Fragments arranged by size:
845 786 688 611 489 486 458 384
330 317 282 142 126 114 105 98
85 80 78 72 49 34 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1884 6660
Size: 1884 4776
HaeII r_GCGC'y
Cuts at: 0 748 1085 1879 6185 6660
Size: 748 337 794 4306 475
Fragments arranged by size:
4306 794 748 475 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1970 2002 3070 3102 3949 3955
Size: 1068 32 870 32 1068 32 847 6
Cuts at: 3955 3981 3987 4144 4176 5545 5577 6660
Size: 26 6 157 32 1369 32 1083
Fragments arranged by size:
1369 1083 1068 1068 870 847 157 32
32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 0 372 957 3864 6660
Size: 372 585 2907 2796
Fragments arranged by size:
2907 2796 585 372
HindIII A'AGCT_T
Cuts at: 0 57 548 1338 3926 6660
Size: 57 491 790 2588 2734
Fragments arranged by size:
2734 2588 790 491 57
HpaI GTT'AAC
Cuts at: 0 957 6660
Size: 957 5703
KpnI G_GTAC'C
Cuts at: 0 2886 3121 6660
Size: 2886 235 3539
Fragments arranged by size:
3539 2886 235
MfeI C'AATT_G
Cuts at: 0 3478 6660
Size: 3478 3182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1052 3437 5561 5636 6660
Size: 881 171 2385 2124 75 1024
Fragments arranged by size:
2385 2124 1024 881 171 75
MscI TGG'CCA
Cuts at: 0 835 2353 3016 3701 3769 5096 5200 6660
Size: 835 1518 663 685 68 1327 104 1460
Fragments arranged by size:
1518 1460 1327 835 685 663 104 68
MslI CAynn'nnrTG
Cuts at: 0 943 1030 1387 1927 1954 2494 3043 6660
Size: 943 87 357 540 27 540 549 3617
Fragments arranged by size:
3617 943 549 540 540 357 87 27
MspA1I CmG'CkG
Cuts at: 0 294 306 691 994 1550 1584 1656 1785
Size: 294 12 385 303 556 34 72 129
Cuts at: 1785 2091 2217 2623 3587 3979 4512 4845 4890
Size: 306 126 406 964 392 533 333 45
Cuts at: 4890 4950 5857 5871 6292 6660
Size: 60 907 14 421 368
Fragments arranged by size:
964 907 556 533 421 406 392 385
368 333 306 303 294 129 126 72
60 45 34 14 12
NaeI GCC'GGC
Cuts at: 0 3535 4493 4700 5377 6158 6660
Size: 3535 958 207 677 781 502
Fragments arranged by size:
3535 958 781 677 502 207
NcoI C'CATG_G
Cuts at: 0 326 938 1481 3770 5201 5279 6660
Size: 326 612 543 2289 1431 78 1381
Fragments arranged by size:
2289 1431 1381 612 543 326 78
NgoMIV G'CCGG_C
Cuts at: 0 3533 4491 4698 5375 6156 6660
Size: 3533 958 207 677 781 504
Fragments arranged by size:
3533 958 781 677 504 207
NsiI A_TGCA'T
Cuts at: 0 3239 6660
Size: 3239 3421
NspI r_CATG'y
Cuts at: 0 397 530 663 3237 3364 3549 3633 4698
Size: 397 133 133 2574 127 185 84 1065
Cuts at: 4698 5670 6396 6660
Size: 972 726 264
Fragments arranged by size:
2574 1065 972 726 397 264 185 133
133 127 84
PasI CC'CwG_GG
Cuts at: 0 250 1753 4459 4482 5020 5980 6171 6660
Size: 250 1503 2706 23 538 960 191 489
Fragments arranged by size:
2706 1503 960 538 489 250 191 23
PciI A'CATG_T
Cuts at: 0 393 526 659 6660
Size: 393 133 133 6001
Fragments arranged by size:
6001 393 133 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 1351 1767 2076 2310 2910 4430 4941 6660
Size: 1351 416 309 234 600 1520 511 1719
Fragments arranged by size:
1719 1520 1351 600 511 416 309 234
PfoI T'CCnGG_A
Cuts at: 0 1229 1451 4207 5546 6590 6660
Size: 1229 222 2756 1339 1044 70
Fragments arranged by size:
2756 1339 1229 1044 222 70
PpuMI rG'GwC_Cy
Cuts at: 0 912 1757 2705 3855 3889 4452 4938 5976
Size: 912 845 948 1150 34 563 486 1038
Cuts at: 5976 6588 6660
Size: 612 72
Fragments arranged by size:
1150 1038 948 912 845 612 563 486
72 34
PsiI TTA'TAA
Cuts at: 0 278 6660
Size: 278 6382
PspOMI G'GGCC_C
Cuts at: 0 3346 3392 3993 4643 5023 5152 5266 5364
Size: 3346 46 601 650 380 129 114 98
Cuts at: 5364 6033 6660
Size: 669 627
Fragments arranged by size:
3346 669 650 627 601 380 129 114
98 46
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3154 3186 6660
Size: 3154 32 3474
Fragments arranged by size:
3474 3154 32
PstI C_TGCA'G
Cuts at: 0 140 1600 1695 1883 2231 2488 3885 4347
Size: 140 1460 95 188 348 257 1397 462
Cuts at: 4347 4374 4691 6199 6660
Size: 27 317 1508 461
Fragments arranged by size:
1508 1460 1397 462 461 348 317 257
188 140 95 27
PvuII CAG'CTG
Cuts at: 0 294 691 994 1550 1656 1785 2217 3979
Size: 294 397 303 556 106 129 432 1762
Cuts at: 3979 4845 5857 6292 6660
Size: 866 1012 435 368
Fragments arranged by size:
1762 1012 866 556 435 432 397 368
303 294 129 106
SacI G_AGCT'C
Cuts at: 0 185 3515 5000 6280 6660
Size: 185 3330 1485 1280 380
Fragments arranged by size:
3330 1485 1280 380 185
SanDI GG'GwC_CC
Cuts at: 0 1757 6660
Size: 1757 4903
SapI GCTCTTCn'nnn_
Cuts at: 0 4717 6041 6660
Size: 4717 1324 619
Fragments arranged by size:
4717 1324 619
ScaI AGT'ACT
Cuts at: 0 2756 6660
Size: 2756 3904
SfcI C'TryA_G
Cuts at: 0 136 1596 1691 1780 1879 2227 2484 3687
Size: 136 1460 95 89 99 348 257 1203
Cuts at: 3687 3881 4343 4370 4614 4687 6195 6660
Size: 194 462 27 244 73 1508 465
Fragments arranged by size:
1508 1460 1203 465 462 348 257 244
194 136 99 95 89 73 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3775 5102 5991 6000 6660
Size: 3775 1327 889 9 660
Fragments arranged by size:
3775 1327 889 660 9
SmaI CCC'GGG
Cuts at: 0 3295 6660
Size: 3295 3365
SmlI C'TyrA_G
Cuts at: 0 589 928 4828 6660
Size: 589 339 3900 1832
Fragments arranged by size:
3900 1832 589 339
SphI G_CATG'C
Cuts at: 0 3364 3549 6660
Size: 3364 185 3111
Fragments arranged by size:
3364 3111 185
SrfI GCCC'GGGC
Cuts at: 0 3295 6660
Size: 3295 3365
SspI AAT'ATT
Cuts at: 0 268 6660
Size: 268 6392
StuI AGG'CCT
Cuts at: 0 718 3946 5758 6003 6219 6660
Size: 718 3228 1812 245 216 441
Fragments arranged by size:
3228 1812 718 441 245 216
StyI C'CwwG_G
Cuts at: 0 326 719 879 938 1481 2336 2510 2825
Size: 326 393 160 59 543 855 174 315
Cuts at: 2825 3215 3770 5027 5201 5279 5972 6302 6660
Size: 390 555 1257 174 78 693 330 358
Fragments arranged by size:
1257 855 693 555 543 393 390 358
330 326 315 174 174 160 78 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1081 1759 2337 3681 3936 4133 4437 6359
Size: 1081 678 578 1344 255 197 304 1922
Cuts at: 6359 6471 6660
Size: 112 189
Fragments arranged by size:
1922 1344 1081 678 578 304 255 197
189 112
TatI w'GTAC_w
Cuts at: 0 503 533 839 2754 2849 3788 4324 6660
Size: 503 30 306 1915 95 939 536 2336
Fragments arranged by size:
2336 1915 939 536 503 306 95 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4041 4073 5156 5188 5922 5954 6660
Size: 4041 32 1083 32 734 32 706
Fragments arranged by size:
4041 1083 734 706 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1984 3082 5863 6660
Size: 1984 1098 2781 797
Fragments arranged by size:
2781 1984 1098 797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 993 3777 3976 4194 5277 5731 6660
Size: 993 2784 199 218 1083 454 929
Fragments arranged by size:
2784 1083 993 929 454 218 199
XmaI C'CCGG_G
Cuts at: 0 3293 6660
Size: 3293 3367
XmnI GAAnn'nnTTC
Cuts at: 0 3382 6660
Size: 3382 3278
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PpuMI PsiI PspOMI PsrI PstI PvuII
SacI SanDI SapI ScaI SfcI SfiI SmaI SmlI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AscI AseI AsiSI AvrII BbeI
BmtI BsaAI BsiWI BsrDI BssHII BstZ17I ClaI EcoRI
EcoRV FseI FspI FspAI KasI MluI NarI NdeI
NheI NotI NruI PacI PmeI PmlI PpiI PshAI
PspXI PvuI RsrII SacII SalI SbfI SexAI SfoI
SgfI SgrAI SnaBI SpeI SwaI XbaI XhoI ZraI