Restriction map for
pF1KA0324
(Linear) (Six-base) MAPSORT of: KIAA0324.seq Check: 9544 from: 1 to: 8256
KIAA0324.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1098 1773 1866 5481 6750 7536 8256
Size: 1098 675 93 3615 1269 786 720
Fragments arranged by size:
3615 1269 1098 786 720 675 93
AatII G_ACGT'C
Cuts at: 0 2827 5064 5361 7782 8256
Size: 2827 2237 297 2421 474
Fragments arranged by size:
2827 2421 2237 474 297
AclI AA'CG_TT
Cuts at: 0 6120 8256
Size: 6120 2136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 2165 3755 3836 4826 4967 5696 6333
Size: 881 1284 1590 81 990 141 729 637
Cuts at: 6333 7747 8256
Size: 1414 509
Fragments arranged by size:
1590 1414 1284 990 881 729 637 509
141 81
AflII C'TTAA_G
Cuts at: 0 1155 8256
Size: 1155 7101
AhdI GACnn_n'nnGTC
Cuts at: 0 8064 8256
Size: 8064 192
AleI CACnn'nnGTG
Cuts at: 0 1560 8256
Size: 1560 6696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3695 7571 8256
Size: 3695 3876 685
Fragments arranged by size:
3876 3695 685
AlwNI CAG_nnn'CTG
Cuts at: 0 493 664 3316 6706 6940 7003 7045 8256
Size: 493 171 2652 3390 234 63 42 1211
Fragments arranged by size:
3390 2652 1211 493 234 171 63 42
ApaI G_GGCC'C
Cuts at: 0 780 4893 7545 8256
Size: 780 4113 2652 711
Fragments arranged by size:
4113 2652 780 711
ApoI r'AATT_y
Cuts at: 0 257 3450 3556 3994 8256
Size: 257 3193 106 438 4262
Fragments arranged by size:
4262 3193 438 257 106
AvaI C'yCGr_G
Cuts at: 0 29 211 470 1199 1225 4242 4362 4886
Size: 29 182 259 729 26 3017 120 524
Cuts at: 4886 5597 6185 8256
Size: 711 588 2071
Fragments arranged by size:
3017 2071 729 711 588 524 259 182
120 29 26
AvrII C'CTAG_G
Cuts at: 0 4477 8256
Size: 4477 3779
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2918 2951 8256
Size: 2918 33 5305
Fragments arranged by size:
5305 2918 33
BamHI G'GATC_C
Cuts at: 0 2398 3091 4618 8256
Size: 2398 693 1527 3638
Fragments arranged by size:
3638 2398 1527 693
BanI G'GyrC_C
Cuts at: 0 40 128 5168 6080 6571 6642 6831 7152
Size: 40 88 5040 912 491 71 189 321
Cuts at: 7152 7500 8256
Size: 348 756
Fragments arranged by size:
5040 912 756 491 348 321 189 88
71 40
BanII G_rGCy'C
Cuts at: 0 780 2725 4514 4893 5033 7272 7545 7696
Size: 780 1945 1789 379 140 2239 273 151
Cuts at: 7696 8256
Size: 560
Fragments arranged by size:
2239 1945 1789 780 560 379 273 151
140
BbeI G_GCGC'C
Cuts at: 0 132 6084 8256
Size: 132 5952 2172
Fragments arranged by size:
5952 2172 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 1013 1235 2336 3293 3584 3761 4181 4319
Size: 1013 222 1101 957 291 177 420 138
Cuts at: 4319 5183 5213 5406 7928 8256
Size: 864 30 193 2522 328
Fragments arranged by size:
2522 1101 1013 957 864 420 328 291
222 193 177 138 30
BbvCI CC'TCA_GC
Cuts at: 0 458 1610 8071 8256
Size: 458 1152 6461 185
Fragments arranged by size:
6461 1152 458 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4446 4480 4591 4625 4711 4745 8256
Size: 4446 34 111 34 86 34 3511
Fragments arranged by size:
4446 3511 111 86 34 34 34
BclI T'GATC_A
Cuts at: 0 3852 4569 7464 8256
Size: 3852 717 2895 792
Fragments arranged by size:
3852 2895 792 717
BglI GCCn_nnn'nGGC
Cuts at: 0 2792 4888 5516 6709 8256
Size: 2792 2096 628 1193 1547
Fragments arranged by size:
2792 2096 1547 1193 628
BglII A'GATC_T
Cuts at: 0 1570 1714 1870 2134 2167 3202 3331 5593
Size: 1570 144 156 264 33 1035 129 2262
Cuts at: 5593 5665 5977 6091 6208 8256
Size: 72 312 114 117 2048
Fragments arranged by size:
2262 2048 1570 1035 312 264 156 144
129 117 114 72 33
BlpI GC'TnA_GC
Cuts at: 0 1112 5238 7955 8256
Size: 1112 4126 2717 301
Fragments arranged by size:
4126 2717 1112 301
Bme1580I G_kGCm'C
Cuts at: 0 471 780 4842 4893 7157 7505 7545 8256
Size: 471 309 4062 51 2264 348 40 711
Fragments arranged by size:
4062 2264 711 471 348 309 51 40
BmgBI CAC'GTC
Cuts at: 0 1591 1963 1996 2128 7451 8256
Size: 1591 372 33 132 5323 805
Fragments arranged by size:
5323 1591 805 372 132 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1233 1527 4640 5743 7547 8256
Size: 1233 294 3113 1103 1804 709
Fragments arranged by size:
3113 1804 1233 1103 709 294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 626 2297 2852 3284 4511 4695 5924
Size: 624 2 1671 555 432 1227 184 1229
Cuts at: 5924 8256
Size: 2332
Fragments arranged by size:
2332 1671 1229 1227 624 555 432 184
2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 238 316 1316 1322 1962 2031 2433
Size: 154 84 78 1000 6 640 69 402
Cuts at: 2433 3293 3641 3917 4316 4670 4730 4862 5293
Size: 860 348 276 399 354 60 132 431
Cuts at: 5293 5591 5981 6053 6144 6200 6263 6299 6957
Size: 298 390 72 91 56 63 36 658
Cuts at: 6957 6963 6980 7047 7100 7307 7656 8229 8256
Size: 6 17 67 53 207 349 573 27
Fragments arranged by size:
1000 860 658 640 573 431 402 399
390 354 349 348 298 276 207 154
132 91 84 78 72 69 67 63
60 56 53 36 27 17 6 6
Bpu10I CC'TnA_GC
Cuts at: 0 458 494 1610 2357 2673 3398 8071 8256
Size: 458 36 1116 747 316 725 4673 185
Fragments arranged by size:
4673 1116 747 725 458 316 185 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4610 5253 5313 5448 8256
Size: 4610 643 60 135 2808
Fragments arranged by size:
4610 2808 643 135 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 322 695 746 839 1493 1610 1814 1847
Size: 322 373 51 93 654 117 204 33
Cuts at: 1847 1907 2078 2285 7349 7418 8255 8256
Size: 60 171 207 5064 69 837 1
Fragments arranged by size:
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117 93 69 60 51 33 1
BsaAI yAC'GTr
Cuts at: 0 435 8256
Size: 435 7821
BsaBI GATnn'nnATC
Cuts at: 0 1524 1782 2856 5769 5946 5982 8256
Size: 1524 258 1074 2913 177 36 2274
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 25 129 2824 5061 5358 5508 6081 7779
Size: 25 104 2695 2237 297 150 573 1698
Cuts at: 7779 8256
Size: 477
Fragments arranged by size:
2695 2237 1698 573 477 297 150 104
25
BsaWI w'CCGG_w
Cuts at: 0 1059 5082 5171 5961 7785 8256
Size: 1059 4023 89 790 1824 471
Fragments arranged by size:
4023 1824 1059 790 471 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1437 1467 6195 6225 6955 6985 7184 7214
Size: 1437 30 4728 30 730 30 199 30
Cuts at: 7214 8167 8197 8227 8256
Size: 953 30 30 29
Fragments arranged by size:
4728 1437 953 730 199 30 30 30
30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 235 340 602 801 1598 2507 2675
Size: 130 105 105 262 199 797 909 168
Cuts at: 2675 2787 2907 4500 4740 5490 6203 6266 6575
Size: 112 120 1593 240 750 713 63 309
Cuts at: 6575 7590 7614 7617 7647 7650 7653 7830 7833
Size: 1015 24 3 30 3 3 177 3
Cuts at: 7833 7848 7851 7854 7857 7860 7863 7875 7878
Size: 15 3 3 3 3 3 12 3
Cuts at: 7878 7902 7914 7917 8232 8256
Size: 24 12 3 315 24
Fragments arranged by size:
1593 1015 909 797 750 713 315 309
262 240 199 177 168 130 120 112
105 105 63 30 24 24 24 15
12 12 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 874 1583 1698 1953 1986 2019 3646 6876
Size: 874 709 115 255 33 33 1627 3230
Cuts at: 6876 6906 7544 7738 7749 7797 8108 8188 8256
Size: 30 638 194 11 48 311 80 68
Fragments arranged by size:
3230 1627 874 709 638 311 255 194
115 80 68 48 33 33 30 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 3128 7013 8256
Size: 1538 1590 3885 1243
Fragments arranged by size:
3885 1590 1538 1243
BsiHKAI G_wGCw'C
Cuts at: 0 4514 8256
Size: 4514 3742
BsmI GAATG_Cn'
Cuts at: 0 6407 8256
Size: 6407 1849
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2598 5685 8256
Size: 2598 3087 2571
Fragments arranged by size:
3087 2598 2571
Bsp1286I G_dGCh'C
Cuts at: 0 471 780 2725 4514 4842 4893 5033 7157
Size: 471 309 1945 1789 328 51 140 2124
Cuts at: 7157 7272 7505 7545 7696 8256
Size: 115 233 40 151 560
Fragments arranged by size:
2124 1945 1789 560 471 328 309 233
151 140 115 51 40
BspEI T'CCGG_A
Cuts at: 0 1059 5082 7785 8256
Size: 1059 4023 2703 471
Fragments arranged by size:
4023 2703 1059 471
BspHI T'CATG_A
Cuts at: 0 6650 8256
Size: 6650 1606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 1098 1335 1646 1736 1773 1829 1866
Size: 584 514 237 311 90 37 56 37
Cuts at: 1866 1889 1925 2213 2267 3398 4307 5481 6750
Size: 23 36 288 54 1131 909 1174 1269
Cuts at: 6750 7536 7992 8256
Size: 786 456 264
Fragments arranged by size:
1269 1174 1131 909 786 584 514 456
311 288 264 237 90 56 54 37
37 36 23
BsrBI CCG'CTC
Cuts at: 0 96 1104 1598 1754 1970 2003 2187 5123
Size: 96 1008 494 156 216 33 184 2936
Cuts at: 5123 5282 5327 5522 5834 6020 6050 6086 6158
Size: 159 45 195 312 186 30 36 72
Cuts at: 6158 6577 7200 7587 8016 8148 8183 8240 8256
Size: 419 623 387 429 132 35 57 16
Fragments arranged by size:
2936 1008 623 494 429 419 387 312
216 195 186 184 159 156 132 96
72 57 45 36 35 33 30 16
BsrDI GCAATG_nn'
Cuts at: 0 3682 8256
Size: 3682 4574
BsrFI r'CCGG_y
Cuts at: 0 5411 5618 6454 7057 8256
Size: 5411 207 836 603 1199
Fragments arranged by size:
5411 1199 836 603 207
BsrGI T'GTAC_A
Cuts at: 0 2 8256
Size: 2 8254
BssSI C'ACGA_G
Cuts at: 0 178 5300 8256
Size: 178 5122 2956
Fragments arranged by size:
5122 2956 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 6706 8256
Size: 6706 1550
BstEII G'GTnAC_C
Cuts at: 0 5468 5964 8256
Size: 5468 496 2292
Fragments arranged by size:
5468 2292 496
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1591 5579 6807 8256
Size: 1591 3988 1228 1449
Fragments arranged by size:
3988 1591 1449 1228
BstYI r'GATC_y
Cuts at: 0 1570 1693 1714 1870 2101 2134 2167 2398
Size: 1570 123 21 156 231 33 33 231
Cuts at: 2398 3091 3202 3331 4095 4129 4618 5374 5593
Size: 693 111 129 764 34 489 756 219
Cuts at: 5593 5665 5728 5770 5875 5947 5977 6091 6163
Size: 72 63 42 105 72 30 114 72
Cuts at: 6163 6208 8256
Size: 45 2048
Fragments arranged by size:
2048 1570 764 756 693 489 231 231
219 156 129 123 114 111 105 72
72 72 63 45 42 34 33 33
30 21
Bsu36I CC'TnA_GG
Cuts at: 0 3281 3515 4658 7715 7967 8053 8256
Size: 3281 234 1143 3057 252 86 203
Fragments arranged by size:
3281 3057 1143 252 234 203 86
BtgI C'CryG_G
Cuts at: 0 2293 2839 5578 8119 8256
Size: 2293 546 2739 2541 137
Fragments arranged by size:
2739 2541 2293 546 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 219 8256
Size: 219 8037
BtsI GCAGTG_nn'
Cuts at: 0 2037 4175 6764 7442 8148 8256
Size: 2037 2138 2589 678 706 108
Fragments arranged by size:
2589 2138 2037 706 678 108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6995 7030 8256
Size: 6995 35 1226
Fragments arranged by size:
6995 1226 35
DraIII CAC_nnn'GTG
Cuts at: 0 4846 8256
Size: 4846 3410
DrdI GACnn_nn'nnGTC
Cuts at: 0 2033 8256
Size: 2033 6223
EaeI y'GGCC_r
Cuts at: 0 1392 1593 1965 1998 6797 6809 8256
Size: 1392 201 372 33 4799 12 1447
Fragments arranged by size:
4799 1447 1392 372 201 33 12
EarI CTCTTCn'nnn_
Cuts at: 0 227 1311 1644 2154 2292 2805 3429 3701
Size: 227 1084 333 510 138 513 624 272
Cuts at: 3701 4212 4686 5508 7319 7635 7723 7804 7851
Size: 511 474 822 1811 316 88 81 47
Cuts at: 7851 7881 7896 7902 8256
Size: 30 15 6 354
Fragments arranged by size:
1811 1084 822 624 513 511 510 474
354 333 316 272 227 138 88 81
47 30 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1788 2286 2450 4973 5454 6139 7605 8256
Size: 1788 498 164 2523 481 685 1466 651
Fragments arranged by size:
2523 1788 1466 685 651 498 481 164
EcoICRI GAG'CTC
Cuts at: 0 4512 8256
Size: 4512 3744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 238 316 881 1316 1322 1962 2031
Size: 154 84 78 565 435 6 640 69
Cuts at: 2031 2165 2433 3293 3641 3755 3836 3917 4316
Size: 134 268 860 348 114 81 81 399
Cuts at: 4316 4670 4730 4826 4862 4967 5293 5591 5696
Size: 354 60 96 36 105 326 298 105
Cuts at: 5696 5981 6053 6144 6200 6263 6299 6333 6957
Size: 285 72 91 56 63 36 34 624
Cuts at: 6957 6963 6980 7047 7100 7307 7656 7747 8229
Size: 6 17 67 53 207 349 91 482
Cuts at: 8229 8256
Size: 27
Fragments arranged by size:
860 640 624 565 482 435 399 354
349 348 326 298 285 268 207 154
134 114 105 105 96 91 91 84
81 81 78 72 69 67 63 60
56 53 36 36 34 27 17 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 896 1434 3284 7406 8256
Size: 896 538 1850 4122 850
Fragments arranged by size:
4122 1850 896 850 538
EcoO109I rG'GnC_Cy
Cuts at: 0 1343 1448 1682 1748 1877 2651 2678 2912
Size: 1343 105 234 66 129 774 27 234
Cuts at: 2912 4382 4890 4936 5315 6014 6056 6481 7018
Size: 1470 508 46 379 699 42 425 537
Cuts at: 7018 7541 7734 8021 8256
Size: 523 193 287 235
Fragments arranged by size:
1470 1343 774 699 537 523 508 425
379 287 235 234 234 193 129 105
66 46 42 27
EcoRI G'AATT_C
Cuts at: 0 3450 8256
Size: 3450 4806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3631 5936 8256
Size: 3631 2305 2320
Fragments arranged by size:
3631 2320 2305
HaeII r_GCGC'y
Cuts at: 0 132 5238 6084 6734 7818 8256
Size: 132 5106 846 650 1084 438
Fragments arranged by size:
5106 1084 846 650 438 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 2629 2661 3187 3219 3251 3310
Size: 430 32 2167 32 526 32 32 59
Cuts at: 3310 3342 3837 3869 4747 4779 5028 5060 5487
Size: 32 495 32 878 32 249 32 427
Cuts at: 5487 5519 6459 6491 8005 8037 8050 8082 8167
Size: 32 940 32 1514 32 13 32 85
Cuts at: 8167 8199 8256
Size: 32 57
Fragments arranged by size:
2167 1514 940 878 526 495 430 427
249 85 59 57 32 32 32 32
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 0 4755 5387 8256
Size: 4755 632 2869
Fragments arranged by size:
4755 2869 632
HindIII A'AGCT_T
Cuts at: 0 2115 4460 8256
Size: 2115 2345 3796
Fragments arranged by size:
3796 2345 2115
KasI G'GCGC_C
Cuts at: 0 128 6080 8256
Size: 128 5952 2176
Fragments arranged by size:
5952 2176 128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 5524 6775 8256
Size: 1219 4305 1251 1481
Fragments arranged by size:
4305 1481 1251 1219
MscI TGG'CCA
Cuts at: 0 6811 8256
Size: 6811 1445
MslI CAynn'nnrTG
Cuts at: 0 1560 7511 8256
Size: 1560 5951 745
Fragments arranged by size:
5951 1560 745
MspA1I CmG'CkG
Cuts at: 0 38 349 4086 5406 6787 6796 6826 6880
Size: 38 311 3737 1320 1381 9 30 54
Cuts at: 6880 6910 8256
Size: 30 1346
Fragments arranged by size:
3737 1381 1346 1320 311 54 38 30
30 9
NaeI GCC'GGC
Cuts at: 0 6456 7059 8256
Size: 6456 603 1197
Fragments arranged by size:
6456 1197 603
NarI GG'CG_CC
Cuts at: 0 129 6081 8256
Size: 129 5952 2175
Fragments arranged by size:
5952 2175 129
NgoMIV G'CCGG_C
Cuts at: 0 6454 7057 8256
Size: 6454 603 1199
Fragments arranged by size:
6454 1199 603
NspI r_CATG'y
Cuts at: 0 1300 6366 7036 7479 8256
Size: 1300 5066 670 443 777
Fragments arranged by size:
5066 1300 777 670 443
PasI CC'CwG_GG
Cuts at: 0 313 334 4596 4835 7148 7495 8256
Size: 313 21 4262 239 2313 347 761
Fragments arranged by size:
4262 2313 761 347 313 239 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 8008 8256
Size: 8008 248
PfoI T'CCnGG_A
Cuts at: 0 170 4332 4389 5491 5704 5878 6058 6535
Size: 170 4162 57 1102 213 174 180 477
Cuts at: 6535 8155 8256
Size: 1620 101
Fragments arranged by size:
4162 1620 1102 477 213 180 174 170
101 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4476 4508 4596 4628 5304 5336 8256
Size: 4476 32 88 32 676 32 2920
Fragments arranged by size:
4476 2920 676 88 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1343 1448 1682 1748 1877 2651 2678 2912
Size: 1343 105 234 66 129 774 27 234
Cuts at: 2912 4382 5315 6014 6056 6481 7018 7734 8021
Size: 1470 933 699 42 425 537 716 287
Cuts at: 8021 8256
Size: 235
Fragments arranged by size:
1470 1343 933 774 716 699 537 425
287 235 234 234 129 105 66 42
27
PshAI GACnn'nnGTC
Cuts at: 0 594 1899 5658 8256
Size: 594 1305 3759 2598
Fragments arranged by size:
3759 2598 1305 594
PspOMI G'GGCC_C
Cuts at: 0 776 4889 7541 8256
Size: 776 4113 2652 715
Fragments arranged by size:
4113 2652 776 715
PspXI vC'TCGA_Gb
Cuts at: 0 211 4362 8256
Size: 211 4151 3894
Fragments arranged by size:
4151 3894 211
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1704 1736 4779 4811 8256
Size: 1704 32 3043 32 3445
Fragments arranged by size:
3445 3043 1704 32 32
PstI C_TGCA'G
Cuts at: 0 849 3260 6391 6449 6702 6792 7574 8256
Size: 849 2411 3131 58 253 90 782 682
Fragments arranged by size:
3131 2411 849 782 682 253 90 58
PvuII CAG'CTG
Cuts at: 0 4086 6787 6880 6910 8256
Size: 4086 2701 93 30 1346
Fragments arranged by size:
4086 2701 1346 93 30
SacI G_AGCT'C
Cuts at: 0 4514 8256
Size: 4514 3742
SanDI GG'GwC_CC
Cuts at: 0 1343 7734 8256
Size: 1343 6391 522
Fragments arranged by size:
6391 1343 522
SapI GCTCTTCn'nnn_
Cuts at: 0 227 1311 8256
Size: 227 1084 6945
Fragments arranged by size:
6945 1084 227
SexAI A'CCwGG_T
Cuts at: 0 936 7010 8256
Size: 936 6074 1246
Fragments arranged by size:
6074 1246 936
SfcI C'TryA_G
Cuts at: 0 845 3256 3437 6387 6445 6698 6788 7403
Size: 845 2411 181 2950 58 253 90 615
Cuts at: 7403 7570 8256
Size: 167 686
Fragments arranged by size:
2950 2411 845 686 615 253 181 167
90 58
SfoI GGC'GCC
Cuts at: 0 130 6082 8256
Size: 130 5952 2174
Fragments arranged by size:
5952 2174 130
SgrAI Cr'CCGG_yG
Cuts at: 0 5618 8256
Size: 5618 2638
SmaI CCC'GGG
Cuts at: 0 31 8256
Size: 31 8225
SmlI C'TyrA_G
Cuts at: 0 211 1155 4242 4362 4625 5268 5328 5463
Size: 211 944 3087 120 263 643 60 135
Cuts at: 5463 5597 8256
Size: 134 2659
Fragments arranged by size:
3087 2659 944 643 263 211 135 134
120 60
SnaBI TAC'GTA
Cuts at: 0 435 8256
Size: 435 7821
SpeI A'CTAG_T
Cuts at: 0 3072 8256
Size: 3072 5184
SphI G_CATG'C
Cuts at: 0 1300 7479 8256
Size: 1300 6179 777
Fragments arranged by size:
6179 1300 777
SspI AAT'ATT
Cuts at: 0 7053 8256
Size: 7053 1203
StuI AGG'CCT
Cuts at: 0 1189 3585 3831 7972 8256
Size: 1189 2396 246 4141 284
Fragments arranged by size:
4141 2396 1189 284 246
StyI C'CwwG_G
Cuts at: 0 869 4477 5164 6414 6914 7079 8256
Size: 869 3608 687 1250 500 165 1177
Fragments arranged by size:
3608 1250 1177 869 687 500 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 821 1475 1802 1835 2066 2394 2448 3281
Size: 821 654 327 33 231 328 54 833
Cuts at: 3281 4721 4848 6447 7013 7223 8054 8256
Size: 1440 127 1599 566 210 831 202
Fragments arranged by size:
1599 1440 833 831 821 654 566 328
327 231 210 202 127 54 33
TatI w'GTAC_w
Cuts at: 0 2 748 3573 8256
Size: 2 746 2825 4683
Fragments arranged by size:
4683 2825 746 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1678 1710 5608 5640 6131 6163 7184 7216
Size: 1678 32 3898 32 491 32 1021 32
Cuts at: 7216 8227 8256
Size: 1011 29
Fragments arranged by size:
3898 1678 1021 1011 491 32 32 32
32 29
Tth111I GACn'n_nGTC
Cuts at: 0 2441 2498 2960 3233 5040 7449 8256
Size: 2441 57 462 273 1807 2409 807
Fragments arranged by size:
2441 2409 1807 807 462 273 57
XbaI T'CTAG_A
Cuts at: 0 1573 1690 1723 1756 1816 1849 1945 2011
Size: 1573 117 33 33 60 33 96 66
Cuts at: 2011 2143 2422 2659 4104 4420 5152 5590 5809
Size: 132 279 237 1445 316 732 438 219
Cuts at: 5809 5842 6088 6424 7285 8256
Size: 33 246 336 861 971
Fragments arranged by size:
1573 1445 971 861 732 438 336 316
279 246 237 219 132 117 96 66
60 33 33 33 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 6825 7107 8256
Size: 6825 282 1149
Fragments arranged by size:
6825 1149 282
XhoI C'TCGA_G
Cuts at: 0 211 4242 4362 5597 8256
Size: 211 4031 120 1235 2659
Fragments arranged by size:
4031 2659 1235 211 120
XmaI C'CCGG_G
Cuts at: 0 29 8256
Size: 29 8227
XmnI GAAnn'nnTTC
Cuts at: 0 885 2787 4257 4611 6249 6294 8256
Size: 885 1902 1470 354 1638 45 1962
Fragments arranged by size:
1962 1902 1638 1470 885 354 45
ZraI GAC'GTC
Cuts at: 0 2825 5062 5359 7780 8256
Size: 2825 2237 297 2421 476
Fragments arranged by size:
2825 2421 2237 476 297
Enzymes that do cut:
AarI AatII AclI AcuI AflII AhdI AleI AlfI
AlwNI ApaI ApoI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuII SacI SanDI SapI SexAI SfcI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AfeI AflIII AgeI AloI ApaLI AscI
AseI AsiSI BciVI BfrBI BmtI BsiEI BsiWI BssHII
BstBI BstZ17I ClaI DraI EagI EcoRV FseI FspI
FspAI HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PsiI
PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI
SrfI SwaI