Restriction map for
pF1KA0318
(Linear) (Six-base) MAPSORT of: KIAA0318.seq Check: 8666 from: 1 to: 3156
KIAA0318.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1196 3156
Size: 1196 1960
AclI AA'CG_TT
Cuts at: 0 1568 3156
Size: 1568 1588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1631 3156
Size: 1631 1525
AfeI AGC'GCT
Cuts at: 0 318 2427 3156
Size: 318 2109 729
Fragments arranged by size:
2109 729 318
AhdI GACnn_n'nnGTC
Cuts at: 0 705 3156
Size: 705 2451
AleI CACnn'nnGTG
Cuts at: 0 512 1642 1987 3156
Size: 512 1130 345 1169
Fragments arranged by size:
1169 1130 512 345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1117 1901 3156
Size: 1117 784 1255
Fragments arranged by size:
1255 1117 784
AlwNI CAG_nnn'CTG
Cuts at: 0 30 54 1735 2380 2521 2608 3156
Size: 30 24 1681 645 141 87 548
Fragments arranged by size:
1681 645 548 141 87 30 24
ApaI G_GGCC'C
Cuts at: 0 1933 3156
Size: 1933 1223
ApaLI G'TGCA_C
Cuts at: 0 1143 1886 3156
Size: 1143 743 1270
Fragments arranged by size:
1270 1143 743
ApoI r'AATT_y
Cuts at: 0 468 3156
Size: 468 2688
AvaI C'yCGr_G
Cuts at: 0 164 495 1747 1776 2018 3156
Size: 164 331 1252 29 242 1138
Fragments arranged by size:
1252 1138 331 242 164 29
BanI G'GyrC_C
Cuts at: 0 669 963 1758 1981 2155 2530 3156
Size: 669 294 795 223 174 375 626
Fragments arranged by size:
795 669 626 375 294 223 174
BanII G_rGCy'C
Cuts at: 0 647 956 1754 1933 2543 3156
Size: 647 309 798 179 610 613
Fragments arranged by size:
798 647 613 610 309 179
BbeI G_GCGC'C
Cuts at: 0 2159 3156
Size: 2159 997
BbsI GAAGACnn'nnnn_
Cuts at: 0 887 2064 3156
Size: 887 1177 1092
Fragments arranged by size:
1177 1092 887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 857 3156
Size: 823 34 2299
Fragments arranged by size:
2299 823 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1548 2023 3156
Size: 1548 475 1133
Fragments arranged by size:
1548 1133 475
BglII A'GATC_T
Cuts at: 0 439 1211 3156
Size: 439 772 1945
Fragments arranged by size:
1945 772 439
BlpI GC'TnA_GC
Cuts at: 0 569 3156
Size: 569 2587
Bme1580I G_kGCm'C
Cuts at: 0 674 1147 1890 1910 1933 1984 2703 3029
Size: 674 473 743 20 23 51 719 326
Cuts at: 3029 3156
Size: 127
Fragments arranged by size:
743 719 674 473 326 127 51 23
20
BmgBI CAC'GTC
Cuts at: 0 858 1167 1260 2342 3156
Size: 858 309 93 1082 814
Fragments arranged by size:
1082 858 814 309 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 664 3156
Size: 664 2492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 796 1420 1542 1690 1835 1936 2120
Size: 193 603 624 122 148 145 101 184
Cuts at: 2120 2480 3156
Size: 360 676
Fragments arranged by size:
676 624 603 360 193 184 148 145
122 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2341 3083 3156
Size: 2341 742 73
Fragments arranged by size:
2341 742 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 1518 1521 1773 2477 2736 3156
Size: 1518 3 252 704 259 420
Fragments arranged by size:
1518 704 420 259 252 3
BsaBI GATnn'nnATC
Cuts at: 0 438 3156
Size: 438 2718
BsaHI Gr'CG_yC
Cuts at: 0 1540 2123 2156 2844 3156
Size: 1540 583 33 688 312
Fragments arranged by size:
1540 688 583 312 33
BsaWI w'CCGG_w
Cuts at: 0 421 3156
Size: 421 2735
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 787 817 1081 1111 2275 2305
Size: 376 30 381 30 264 30 1164 30
Cuts at: 2305 3156
Size: 851
Fragments arranged by size:
1164 851 381 376 264 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 637 1291 1294 2131 2163 2287 2296 2299
Size: 637 654 3 837 32 124 9 3
Cuts at: 2299 2629 2632 2770 3156
Size: 330 3 138 386
Fragments arranged by size:
837 654 637 386 330 138 124 32
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 210 253 726 947 1035 1220 1458 1489
Size: 210 43 473 221 88 185 238 31
Cuts at: 1489 1954 2332 2373 3156
Size: 465 378 41 783
Fragments arranged by size:
783 473 465 378 238 221 210 185
88 43 41 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1068 1123 1769 3156
Size: 715 353 55 646 1387
Fragments arranged by size:
1387 715 646 353 55
BsiEI CG_ry'CG
Cuts at: 0 341 1643 1945 3156
Size: 341 1302 302 1211
Fragments arranged by size:
1302 1211 341 302
BsiHKAI G_wGCw'C
Cuts at: 0 647 788 1147 1754 1841 1890 2543 3156
Size: 647 141 359 607 87 49 653 613
Fragments arranged by size:
653 647 613 607 359 141 87 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2213 3156
Size: 2213 943
Bsp1286I G_dGCh'C
Cuts at: 0 647 674 788 956 1147 1754 1841 1890
Size: 647 27 114 168 191 607 87 49
Cuts at: 1890 1910 1933 1984 2543 2703 3029 3156
Size: 20 23 51 559 160 326 127
Fragments arranged by size:
647 607 559 326 191 168 160 127
114 87 51 49 27 23 20
BspHI T'CATG_A
Cuts at: 0 275 3156
Size: 275 2881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 215 1196 1791 2042 3156
Size: 164 51 981 595 251 1114
Fragments arranged by size:
1114 981 595 251 164 51
BsrBI CCG'CTC
Cuts at: 0 2583 3156
Size: 2583 573
BsrFI r'CCGG_y
Cuts at: 0 1573 3156
Size: 1573 1583
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 1735 3156
Size: 30 1705 1421
Fragments arranged by size:
1705 1421 30
BstEII G'GTnAC_C
Cuts at: 0 1494 3156
Size: 1494 1662
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 974 2013 3156
Size: 684 290 1039 1143
Fragments arranged by size:
1143 1039 684 290
BstYI r'GATC_y
Cuts at: 0 418 439 1211 2552 3156
Size: 418 21 772 1341 604
Fragments arranged by size:
1341 772 604 418 21
BtgI C'CryG_G
Cuts at: 0 245 1384 1625 1643 1718 1925 2012 2214
Size: 245 1139 241 18 75 207 87 202
Cuts at: 2214 2418 2685 3156
Size: 204 267 471
Fragments arranged by size:
1139 471 267 245 241 207 204 202
87 75 18
BtsI GCAGTG_nn'
Cuts at: 0 1111 1147 3156
Size: 1111 36 2009
Fragments arranged by size:
2009 1111 36
DraI TTT'AAA
Cuts at: 0 2631 3156
Size: 2631 525
DrdI GACnn_nn'nnGTC
Cuts at: 0 3064 3156
Size: 3064 92
EaeI y'GGCC_r
Cuts at: 0 654 1364 1640 2039 3156
Size: 654 710 276 399 1117
Fragments arranged by size:
1117 710 654 399 276
EagI C'GGCC_G
Cuts at: 0 1640 3156
Size: 1640 1516
EarI CTCTTCn'nnn_
Cuts at: 0 116 2504 2531 3130 3156
Size: 116 2388 27 599 26
Fragments arranged by size:
2388 599 116 27 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 384 1206 1692 3156
Size: 384 822 486 1464
Fragments arranged by size:
1464 822 486 384
EcoICRI GAG'CTC
Cuts at: 0 645 1752 2541 3156
Size: 645 1107 789 615
Fragments arranged by size:
1107 789 645 615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 796 1420 1542 1631 1690 1835 1936
Size: 193 603 624 122 89 59 145 101
Cuts at: 1936 2120 2480 3156
Size: 184 360 676
Fragments arranged by size:
676 624 603 360 193 184 145 122
101 89 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1273 3156
Size: 1273 1883
EcoO109I rG'GnC_Cy
Cuts at: 0 51 168 848 1453 1846 1930 2441 3156
Size: 51 117 680 605 393 84 511 715
Fragments arranged by size:
715 680 605 511 393 117 84 51
EcoRI G'AATT_C
Cuts at: 0 468 3156
Size: 468 2688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 423 1440 3088 3156
Size: 423 1017 1648 68
Fragments arranged by size:
1648 1017 423 68
HaeII r_GCGC'y
Cuts at: 0 320 2159 2429 3156
Size: 320 1839 270 727
Fragments arranged by size:
1839 727 320 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 691 723 773 787 805 819
Size: 376 32 283 32 50 14 18 14
Cuts at: 819 869 901 2751 2783 3070 3102 3156
Size: 50 32 1850 32 287 32 54
Fragments arranged by size:
1850 376 287 283 54 50 50 32
32 32 32 32 18 14 14
KasI G'GCGC_C
Cuts at: 0 2155 3156
Size: 2155 1001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 701 3156
Size: 17 684 2455
Fragments arranged by size:
2455 684 17
MscI TGG'CCA
Cuts at: 0 656 1366 3156
Size: 656 710 1790
Fragments arranged by size:
1790 710 656
MslI CAynn'nnrTG
Cuts at: 0 512 818 972 1099 1642 1987 3156
Size: 512 306 154 127 543 345 1169
Fragments arranged by size:
1169 543 512 345 306 154 127
MspA1I CmG'CkG
Cuts at: 0 30 1398 2307 2336 3156
Size: 30 1368 909 29 820
Fragments arranged by size:
1368 909 820 30 29
NarI GG'CG_CC
Cuts at: 0 2156 3156
Size: 2156 1000
NcoI C'CATG_G
Cuts at: 0 2012 2214 3156
Size: 2012 202 942
Fragments arranged by size:
2012 942 202
NspI r_CATG'y
Cuts at: 0 1914 3156
Size: 1914 1242
PasI CC'CwG_GG
Cuts at: 0 1707 3156
Size: 1707 1449
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 3156
Size: 54 3102
PfoI T'CCnGG_A
Cuts at: 0 791 1519 3156
Size: 791 728 1637
Fragments arranged by size:
1637 791 728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 1267 1299 2362 2394 3156
Size: 613 32 622 32 1063 32 762
Fragments arranged by size:
1063 762 622 613 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 168 848 1453 1846 2441 3156
Size: 168 680 605 393 595 715
Fragments arranged by size:
715 680 605 595 393 168
PspOMI G'GGCC_C
Cuts at: 0 1929 3156
Size: 1929 1227
PstI C_TGCA'G
Cuts at: 0 35 98 1142 1452 1731 2385 2613 3156
Size: 35 63 1044 310 279 654 228 543
Fragments arranged by size:
1044 654 543 310 279 228 63 35
PvuI CG_AT'CG
Cuts at: 0 341 3156
Size: 341 2815
PvuII CAG'CTG
Cuts at: 0 30 2307 3156
Size: 30 2277 849
Fragments arranged by size:
2277 849 30
SacI G_AGCT'C
Cuts at: 0 647 1754 2543 3156
Size: 647 1107 789 613
Fragments arranged by size:
1107 789 647 613
SanDI GG'GwC_CC
Cuts at: 0 848 1846 3156
Size: 848 998 1310
Fragments arranged by size:
1310 998 848
SapI GCTCTTCn'nnn_
Cuts at: 0 2531 3156
Size: 2531 625
SbfI CC_TGCA'GG
Cuts at: 0 1452 3156
Size: 1452 1704
SfcI C'TryA_G
Cuts at: 0 31 94 525 1138 1251 1448 1727 2381
Size: 31 63 431 613 113 197 279 654
Cuts at: 2381 2609 3156
Size: 228 547
Fragments arranged by size:
654 613 547 431 279 228 197 113
63 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2023 3156
Size: 2023 1133
SfoI GGC'GCC
Cuts at: 0 2157 3156
Size: 2157 999
SmaI CCC'GGG
Cuts at: 0 166 2020 3156
Size: 166 1854 1136
Fragments arranged by size:
1854 1136 166
SmlI C'TyrA_G
Cuts at: 0 2356 3098 3156
Size: 2356 742 58
Fragments arranged by size:
2356 742 58
StuI AGG'CCT
Cuts at: 0 1423 1870 3020 3156
Size: 1423 447 1150 136
Fragments arranged by size:
1423 1150 447 136
StyI C'CwwG_G
Cuts at: 0 104 1676 2006 2012 2214 3156
Size: 104 1572 330 6 202 942
Fragments arranged by size:
1572 942 330 202 104 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 564 1968 3156
Size: 202 362 1404 1188
Fragments arranged by size:
1404 1188 362 202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 3123 3155 3156
Size: 126 32 2965 32 1
Fragments arranged by size:
2965 126 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 0 1291 3156
Size: 1291 1865
XmaI C'CCGG_G
Cuts at: 0 164 2018 3156
Size: 164 1854 1138
Fragments arranged by size:
1854 1138 164
Enzymes that do cut:
AarI AclI AcuI AfeI AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BcgI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuI PvuII SacI SanDI
SapI SbfI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BmrI BmtI Bpu10I BsaAI BsiWI BsmI BspEI
BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI RsrII SacII SalI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XcmI XhoI XmnI ZraI