Restriction map for
pF1KA0310
(Linear) (Six-base) MAPSORT of: KIAA0310.seq Check: 1467 from: 1 to: 7071
KIAA0310.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2913 4755 5634 5645 7071
Size: 2913 1842 879 11 1426
Fragments arranged by size:
2913 1842 1426 879 11
AatII G_ACGT'C
Cuts at: 0 5153 6587 7071
Size: 5153 1434 484
Fragments arranged by size:
5153 1434 484
AccI GT'mk_AC
Cuts at: 0 174 3317 3572 5033 7071
Size: 174 3143 255 1461 2038
Fragments arranged by size:
3143 2038 1461 255 174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 470 719 1088 1658 2108 2392 3737
Size: 374 96 249 369 570 450 284 1345
Cuts at: 3737 4388 7071
Size: 651 2683
Fragments arranged by size:
2683 1345 651 570 450 374 369 284
249 96
AflII C'TTAA_G
Cuts at: 0 2991 7071
Size: 2991 4080
AflIII A'CryG_T
Cuts at: 0 1248 3020 4460 6539 7071
Size: 1248 1772 1440 2079 532
Fragments arranged by size:
2079 1772 1440 1248 532
AgeI A'CCGG_T
Cuts at: 0 1836 7071
Size: 1836 5235
AhdI GACnn_n'nnGTC
Cuts at: 0 1560 2605 4593 5154 7071
Size: 1560 1045 1988 561 1917
Fragments arranged by size:
1988 1917 1560 1045 561
AleI CACnn'nnGTG
Cuts at: 0 1339 2887 2920 3830 5474 5622 7071
Size: 1339 1548 33 910 1644 148 1449
Fragments arranged by size:
1644 1548 1449 1339 910 148 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 2938 3083 7023 7071
Size: 1357 1581 145 3940 48
Fragments arranged by size:
3940 1581 1357 145 48
AlwNI CAG_nnn'CTG
Cuts at: 0 448 462 1268 1623 3180 3333 3433 3465
Size: 448 14 806 355 1557 153 100 32
Cuts at: 3465 5373 5971 6775 7071
Size: 1908 598 804 296
Fragments arranged by size:
1908 1557 806 804 598 448 355 296
153 100 32 14
ApaI G_GGCC'C
Cuts at: 0 778 1472 2144 5936 6958 7071
Size: 778 694 672 3792 1022 113
Fragments arranged by size:
3792 1022 778 694 672 113
ApaLI G'TGCA_C
Cuts at: 0 1546 2009 2056 7071
Size: 1546 463 47 5015
Fragments arranged by size:
5015 1546 463 47
ApoI r'AATT_y
Cuts at: 0 1387 4330 4614 6132 6145 6423 7071
Size: 1387 2943 284 1518 13 278 648
Fragments arranged by size:
2943 1518 1387 648 284 278 13
AseI AT'TA_AT
Cuts at: 0 4535 7071
Size: 4535 2536
AvaI C'yCGr_G
Cuts at: 0 672 1013 1446 1660 1779 2110 3646 3652
Size: 672 341 433 214 119 331 1536 6
Cuts at: 3652 3668 3931 4491 4840 4976 5826 5832 5928
Size: 16 263 560 349 136 850 6 96
Cuts at: 5928 6165 6216 7071
Size: 237 51 855
Fragments arranged by size:
1536 855 850 672 560 433 349 341
331 263 237 214 136 119 96 51
16 6 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3714 3747 7071
Size: 3714 33 3324
Fragments arranged by size:
3714 3324 33
BamHI G'GATC_C
Cuts at: 0 156 495 5290 7071
Size: 156 339 4795 1781
Fragments arranged by size:
4795 1781 339 156
BanI G'GyrC_C
Cuts at: 0 720 1341 2079 2914 2922 3099 3183 4216
Size: 720 621 738 835 8 177 84 1033
Cuts at: 4216 5377 5802 6093 6174 7071
Size: 1161 425 291 81 897
Fragments arranged by size:
1161 1033 897 835 738 720 621 425
291 177 84 81 8
BanII G_rGCy'C
Cuts at: 0 326 545 778 950 1113 1424 1472 1524
Size: 326 219 233 172 163 311 48 52
Cuts at: 1524 1786 2144 2161 2622 2979 3171 3653 5697
Size: 262 358 17 461 357 192 482 2044
Cuts at: 5697 5775 5818 5833 5936 5993 6067 6531 6573
Size: 78 43 15 103 57 74 464 42
Cuts at: 6573 6788 6958 7027 7071
Size: 215 170 69 44
Fragments arranged by size:
2044 482 464 461 358 357 326 311
262 233 219 215 192 172 170 163
103 78 74 69 57 52 48 44
43 42 17 15
BbeI G_GCGC'C
Cuts at: 0 3103 6097 7071
Size: 3103 2994 974
Fragments arranged by size:
3103 2994 974
BbsI GAAGACnn'nnnn_
Cuts at: 0 2969 4597 7071
Size: 2969 1628 2474
Fragments arranged by size:
2969 2474 1628
BbvCI CC'TCA_GC
Cuts at: 0 614 2258 4108 5853 7071
Size: 614 1644 1850 1745 1218
Fragments arranged by size:
1850 1745 1644 1218 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3748 3782 6156 6190 6925 6959 7071
Size: 3748 34 2374 34 735 34 112
Fragments arranged by size:
3748 2374 735 112 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2085 2111 3715 7071
Size: 2085 26 1604 3356
Fragments arranged by size:
3356 2085 1604 26
BclI T'GATC_A
Cuts at: 0 2499 5531 7071
Size: 2499 3032 1540
Fragments arranged by size:
3032 2499 1540
BglI GCCn_nnn'nGGC
Cuts at: 0 675 2348 3155 3342 3657 5931 5997 7071
Size: 675 1673 807 187 315 2274 66 1074
Fragments arranged by size:
2274 1673 1074 807 675 315 187 66
BlpI GC'TnA_GC
Cuts at: 0 1605 2492 2519 6121 6992 7071
Size: 1605 887 27 3602 871 79
Fragments arranged by size:
3602 1605 887 871 79 27
Bme1580I G_kGCm'C
Cuts at: 0 725 778 1080 1472 1493 1550 2013 2060
Size: 725 53 302 392 21 57 463 47
Cuts at: 2060 2082 2144 5936 6958 7071
Size: 22 62 3792 1022 113
Fragments arranged by size:
3792 1022 725 463 392 302 113 62
57 53 47 22 21
BmgBI CAC'GTC
Cuts at: 0 4463 7071
Size: 4463 2608
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 431 821 942 1060 3822 5361 5553
Size: 208 223 390 121 118 2762 1539 192
Cuts at: 5553 5908 7071
Size: 355 1163
Fragments arranged by size:
2762 1539 1163 390 355 223 208 192
121 118
BmtI G_CTAG'C
Cuts at: 0 83 7071
Size: 83 6988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1176 7071
Size: 1176 5895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1197 1996 2264 2462 2595 3765 3972
Size: 32 1165 799 268 198 133 1170 207
Cuts at: 3972 4169 5693 5959 6007 6034 6545 6822 7071
Size: 197 1524 266 48 27 511 277 249
Fragments arranged by size:
1524 1170 1165 799 511 277 268 266
249 207 198 197 133 48 32 27
Bpu10I CC'TnA_GC
Cuts at: 0 556 614 949 2258 2680 4108 5819 5853
Size: 556 58 335 1309 422 1428 1711 34
Cuts at: 5853 7071
Size: 1218
Fragments arranged by size:
1711 1428 1309 1218 556 422 335 58
34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2392 7011 7071
Size: 2392 4619 60
Fragments arranged by size:
4619 2392 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 1723 1903 1993 4001 4679 4843 5377 6261
Size: 1723 180 90 2008 678 164 534 884
Cuts at: 6261 7071
Size: 810
Fragments arranged by size:
2008 1723 884 810 678 534 180 164
90
BsaAI yAC'GTr
Cuts at: 0 1251 5072 5624 6901 7071
Size: 1251 3821 552 1277 170
Fragments arranged by size:
3821 1277 1251 552 170
BsaHI Gr'CG_yC
Cuts at: 0 3100 5150 6094 6584 7071
Size: 3100 2050 944 490 487
Fragments arranged by size:
3100 2050 944 490 487
BsaWI w'CCGG_w
Cuts at: 0 1516 1836 3534 3863 5047 7071
Size: 1516 320 1698 329 1184 2024
Fragments arranged by size:
2024 1698 1516 1184 329 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 167 197 1426 1456 2195 2225 4663 4693
Size: 167 30 1229 30 739 30 2438 30
Cuts at: 4693 6032 6062 6518 6548 7071
Size: 1339 30 456 30 523
Fragments arranged by size:
2438 1339 1229 739 523 456 167 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1165 2410 2413 3338 3672 4489 4789 4792
Size: 1165 1245 3 925 334 817 300 3
Cuts at: 4792 5617 6457 6825 6875 7071
Size: 825 840 368 50 196
Fragments arranged by size:
1245 1165 925 840 825 817 368 334
300 196 50 3 3
BseYI C'CCAG_C
Cuts at: 0 37 240 591 712 813 1057 1291 1766
Size: 37 203 351 121 101 244 234 475
Cuts at: 1766 2160 2789 3170 3191 3661 4793 5579 6605
Size: 394 629 381 21 470 1132 786 1026
Cuts at: 6605 6961 7071
Size: 356 110
Fragments arranged by size:
1132 1026 786 629 475 470 394 381
356 351 244 234 203 121 110 101
37 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 673 1953 2356 2891 3419 4054 5566
Size: 443 230 1280 403 535 528 635 1512
Cuts at: 5566 5792 5957 7071
Size: 226 165 1114
Fragments arranged by size:
1512 1280 1114 635 535 528 443 403
230 226 165
BsiEI CG_ry'CG
Cuts at: 0 549 7071
Size: 549 6522
BsiHKAI G_wGCw'C
Cuts at: 0 357 950 980 1113 1336 1550 2013 2060
Size: 357 593 30 133 223 214 463 47
Cuts at: 2060 2850 3911 4055 5825 6573 7027 7071
Size: 790 1061 144 1770 748 454 44
Fragments arranged by size:
1770 1061 790 748 593 463 454 357
223 214 144 133 47 44 30
BsiWI C'GTAC_G
Cuts at: 0 1036 7071
Size: 1036 6035
BsmBI CGTCTCn'nnnn_
Cuts at: 0 90 512 833 5075 7071
Size: 90 422 321 4242 1996
Fragments arranged by size:
4242 1996 422 321 90
Bsp1286I G_dGCh'C
Cuts at: 0 326 357 545 725 778 950 980 1080
Size: 326 31 188 180 53 172 30 100
Cuts at: 1080 1113 1336 1424 1472 1493 1524 1550 1786
Size: 33 223 88 48 21 31 26 236
Cuts at: 1786 2013 2060 2082 2144 2161 2622 2850 2979
Size: 227 47 22 62 17 461 228 129
Cuts at: 2979 3171 3653 3911 4055 5697 5775 5818 5825
Size: 192 482 258 144 1642 78 43 7
Cuts at: 5825 5833 5936 5993 6067 6531 6573 6788 6958
Size: 8 103 57 74 464 42 215 170
Cuts at: 6958 7027 7071
Size: 69 44
Fragments arranged by size:
1642 482 464 461 326 258 236 228
227 223 215 192 188 180 172 170
144 129 103 100 88 78 74 69
62 57 53 48 47 44 43 42
33 31 31 30 26 22 21 17
8 7
BspEI T'CCGG_A
Cuts at: 0 5047 7071
Size: 5047 2024
BspHI T'CATG_A
Cuts at: 0 783 2556 4982 7071
Size: 783 1773 2426 2089
Fragments arranged by size:
2426 2089 1773 783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2610 2913 3117 4755 5395 5634 5645 7071
Size: 2610 303 204 1638 640 239 11 1426
Fragments arranged by size:
2610 1638 1426 640 303 239 204 11
BsrBI CCG'CTC
Cuts at: 0 3657 5652 6253 7071
Size: 3657 1995 601 818
Fragments arranged by size:
3657 1995 818 601
BsrDI GCAATG_nn'
Cuts at: 0 1302 4664 4916 7071
Size: 1302 3362 252 2155
Fragments arranged by size:
3362 2155 1302 252
BsrFI r'CCGG_y
Cuts at: 0 147 980 1836 3485 4031 4159 4416 6618
Size: 147 833 856 1649 546 128 257 2202
Cuts at: 6618 7071
Size: 453
Fragments arranged by size:
2202 1649 856 833 546 453 257 147
128
BsrGI T'GTAC_A
Cuts at: 0 3023 7071
Size: 3023 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1268 1545 2845 7062 7071
Size: 1268 277 1300 4217 9
Fragments arranged by size:
4217 1300 1268 277 9
BstBI TT'CG_AA
Cuts at: 0 1832 7071
Size: 1832 5239
BstEII G'GTnAC_C
Cuts at: 0 585 7071
Size: 585 6486
BstXI CCAn_nnnn'nTGG
Cuts at: 0 526 641 3812 5166 5628 7071
Size: 526 115 3171 1354 462 1443
Fragments arranged by size:
3171 1443 1354 526 462 115
BstYI r'GATC_y
Cuts at: 0 156 495 3801 5290 7071
Size: 156 339 3306 1489 1781
Fragments arranged by size:
3306 1781 1489 339 156
Bsu36I CC'TnA_GG
Cuts at: 0 3458 6845 7071
Size: 3458 3387 226
Fragments arranged by size:
3458 3387 226
BtgI C'CryG_G
Cuts at: 0 1587 1752 2045 3415 4292 4649 5117 5174
Size: 1587 165 293 1370 877 357 468 57
Cuts at: 5174 7071
Size: 1897
Fragments arranged by size:
1897 1587 1370 877 468 357 293 165
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1132 2305 5469 7071
Size: 1132 1173 3164 1602
Fragments arranged by size:
3164 1602 1173 1132
BtsI GCAGTG_nn'
Cuts at: 0 125 1534 1936 2706 4245 4763 5713 5920
Size: 125 1409 402 770 1539 518 950 207
Cuts at: 5920 7071
Size: 1151
Fragments arranged by size:
1539 1409 1151 950 770 518 402 207
125
ClaI AT'CG_AT
Cuts at: 0 1697 6388 7071
Size: 1697 4691 683
Fragments arranged by size:
4691 1697 683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 212 247 7071
Size: 212 35 6824
Fragments arranged by size:
6824 212 35
DraI TTT'AAA
Cuts at: 0 6428 7071
Size: 6428 643
DraIII CAC_nnn'GTG
Cuts at: 0 4152 6016 7062 7071
Size: 4152 1864 1046 9
Fragments arranged by size:
4152 1864 1046 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 24 583 7071
Size: 24 559 6488
Fragments arranged by size:
6488 559 24
EaeI y'GGCC_r
Cuts at: 0 875 3387 3418 3467 3691 4088 4502 4881
Size: 875 2512 31 49 224 397 414 379
Cuts at: 4881 5453 5612 5885 6761 7044 7071
Size: 572 159 273 876 283 27
Fragments arranged by size:
2512 876 875 572 414 397 379 283
273 224 159 49 31 27
EarI CTCTTCn'nnn_
Cuts at: 0 831 2110 2279 2855 4016 4766 5485 5575
Size: 831 1279 169 576 1161 750 719 90
Cuts at: 5575 5594 6434 6546 7071
Size: 19 840 112 525
Fragments arranged by size:
1279 1161 840 831 750 719 576 525
169 112 90 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1223 1909 5455 7071
Size: 1223 686 3546 1616
Fragments arranged by size:
3546 1616 1223 686
EcoICRI GAG'CTC
Cuts at: 0 948 1111 6571 7025 7071
Size: 948 163 5460 454 46
Fragments arranged by size:
5460 948 454 163 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 374 470 719 1088 1197 1658 1996
Size: 32 342 96 249 369 109 461 338
Cuts at: 1996 2108 2264 2392 2462 2595 3737 3765 3972
Size: 112 156 128 70 133 1142 28 207
Cuts at: 3972 4169 4388 5693 5959 6007 6034 6545 6822
Size: 197 219 1305 266 48 27 511 277
Cuts at: 6822 7071
Size: 249
Fragments arranged by size:
1305 1142 511 461 369 342 338 277
266 249 249 219 207 197 156 133
128 112 109 96 70 48 32 28
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 769 1223 1270 2994 4406 5641 6837 6947
Size: 769 454 47 1724 1412 1235 1196 110
Cuts at: 6947 7071
Size: 124
Fragments arranged by size:
1724 1412 1235 1196 769 454 124 110
47
EcoO109I rG'GnC_Cy
Cuts at: 0 313 703 774 775 1275 1288 1469 1613
Size: 313 390 71 1 500 13 181 144
Cuts at: 1613 2132 2140 2403 3462 3599 3846 4173 4495
Size: 519 8 263 1059 137 247 327 322
Cuts at: 4495 5199 5932 5933 5947 5968 6189 6220 6517
Size: 704 733 1 14 21 221 31 297
Cuts at: 6517 6602 6739 6756 6954 6955 7071
Size: 85 137 17 198 1 116
Fragments arranged by size:
1059 733 704 519 500 390 327 322
313 297 263 247 221 198 181 144
137 137 116 85 71 31 21 17
14 13 8 1 1 1
EcoRV GAT'ATC
Cuts at: 0 3712 7071
Size: 3712 3359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3017 4334 5298 7071
Size: 3017 1317 964 1773
Fragments arranged by size:
3017 1773 1317 964
HaeII r_GCGC'y
Cuts at: 0 184 2239 3103 6097 7071
Size: 184 2055 864 2994 974
Fragments arranged by size:
2994 2055 974 864 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 167 199 1195 1227 1426 1458 1546 1578
Size: 167 32 996 32 199 32 88 32
Cuts at: 1578 2195 2227 2278 2310 2591 2623 3945 3977
Size: 617 32 51 32 281 32 1322 32
Cuts at: 3977 4380 4412 4579 4611 4765 4797 5140 5172
Size: 403 32 167 32 154 32 343 32
Cuts at: 5172 6032 6064 7071
Size: 860 32 1007
Fragments arranged by size:
1322 1007 996 860 617 403 343 281
199 167 167 154 88 51 32 32
32 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 3318 6582 7071
Size: 3318 3264 489
Fragments arranged by size:
3318 3264 489
HindIII A'AGCT_T
Cuts at: 0 1670 5275 6359 7071
Size: 1670 3605 1084 712
Fragments arranged by size:
3605 1670 1084 712
KasI G'GCGC_C
Cuts at: 0 3099 6093 7071
Size: 3099 2994 978
Fragments arranged by size:
3099 2994 978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1632 2689 5153 5723 6081 6493 7071
Size: 151 1481 1057 2464 570 358 412 578
Fragments arranged by size:
2464 1481 1057 578 570 412 358 151
MscI TGG'CCA
Cuts at: 0 877 3420 4090 4504 5455 5887 6763 7046
Size: 877 2543 670 414 951 432 876 283
Cuts at: 7046 7071
Size: 25
Fragments arranged by size:
2543 951 877 876 670 432 414 283
25
MslI CAynn'nnrTG
Cuts at: 0 290 524 578 1339 2887 2920 3830 4349
Size: 290 234 54 761 1548 33 910 519
Cuts at: 4349 5474 5622 5687 7071
Size: 1125 148 65 1384
Fragments arranged by size:
1548 1384 1125 910 761 519 290 234
148 65 54 33
MspA1I CmG'CkG
Cuts at: 0 716 1355 1486 1942 3333 3397 5583 6609
Size: 716 639 131 456 1391 64 2186 1026
Cuts at: 6609 6965 6996 7071
Size: 356 31 75
Fragments arranged by size:
2186 1391 1026 716 639 456 356 131
75 64 31
NaeI GCC'GGC
Cuts at: 0 4418 6620 7071
Size: 4418 2202 451
Fragments arranged by size:
4418 2202 451
NarI GG'CG_CC
Cuts at: 0 3100 6094 7071
Size: 3100 2994 977
Fragments arranged by size:
3100 2994 977
NcoI C'CATG_G
Cuts at: 0 4292 5117 5174 7071
Size: 4292 825 57 1897
Fragments arranged by size:
4292 1897 825 57
NgoMIV G'CCGG_C
Cuts at: 0 4416 6618 7071
Size: 4416 2202 453
Fragments arranged by size:
4416 2202 453
NheI G'CTAG_C
Cuts at: 0 79 7071
Size: 79 6992
NruI TCG'CGA
Cuts at: 0 5488 7071
Size: 5488 1583
NspI r_CATG'y
Cuts at: 0 295 361 537 2064 6543 7071
Size: 295 66 176 1527 4479 528
Fragments arranged by size:
4479 1527 528 295 176 66
PasI CC'CwG_GG
Cuts at: 0 1219 2136 3144 5373 5775 5965 6073 6196
Size: 1219 917 1008 2229 402 190 108 123
Cuts at: 6196 6514 6855 7071
Size: 318 341 216
Fragments arranged by size:
2229 1219 1008 917 402 341 318 216
190 123 108
PciI A'CATG_T
Cuts at: 0 6539 7071
Size: 6539 532
PflMI CCAn_nnn'nTGG
Cuts at: 0 1050 1623 2200 4152 4261 4440 4524 5373
Size: 1050 573 577 1952 109 179 84 849
Cuts at: 5373 7071
Size: 1698
Fragments arranged by size:
1952 1698 1050 849 577 573 179 109
84
PfoI T'CCnGG_A
Cuts at: 0 486 960 2572 2853 5158 5984 6050 6522
Size: 486 474 1612 281 2305 826 66 472
Cuts at: 6522 7018 7071
Size: 496 53
Fragments arranged by size:
2305 1612 826 496 486 474 472 281
66 53
PmlI CAC'GTG
Cuts at: 0 1251 5072 5624 6901 7071
Size: 1251 3821 552 1277 170
Fragments arranged by size:
3821 1277 1251 552 170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2305 2337 6529 6555 6561 6587 7071
Size: 2305 32 4192 26 6 26 484
Fragments arranged by size:
4192 2305 484 32 26 26 6
PpuMI rG'GwC_Cy
Cuts at: 0 313 703 2132 3462 3846 4495 5947 6220
Size: 313 390 1429 1330 384 649 1452 273
Cuts at: 6220 6602 7071
Size: 382 469
Fragments arranged by size:
1452 1429 1330 649 469 390 384 382
313 273
PshAI GACnn'nnGTC
Cuts at: 0 1915 3323 6218 7071
Size: 1915 1408 2895 853
Fragments arranged by size:
2895 1915 1408 853
PspOMI G'GGCC_C
Cuts at: 0 774 1468 2140 5932 6954 7071
Size: 774 694 672 3792 1022 117
Fragments arranged by size:
3792 1022 774 694 672 117
PspXI vC'TCGA_Gb
Cuts at: 0 5826 6165 7071
Size: 5826 339 906
Fragments arranged by size:
5826 906 339
PstI C_TGCA'G
Cuts at: 0 629 2229 2471 2607 4457 5027 5642 6032
Size: 629 1600 242 136 1850 570 615 390
Cuts at: 6032 6747 6807 6951 7071
Size: 715 60 144 120
Fragments arranged by size:
1850 1600 715 629 615 570 390 242
144 136 120 60
PvuII CAG'CTG
Cuts at: 0 1486 1942 3333 3397 5583 6996 7071
Size: 1486 456 1391 64 2186 1413 75
Fragments arranged by size:
2186 1486 1413 1391 456 75 64
SacI G_AGCT'C
Cuts at: 0 950 1113 6573 7027 7071
Size: 950 163 5460 454 44
Fragments arranged by size:
5460 950 454 163 44
SalI G'TCGA_C
Cuts at: 0 3316 7071
Size: 3316 3755
SanDI GG'GwC_CC
Cuts at: 0 313 3846 4495 6220 6602 7071
Size: 313 3533 649 1725 382 469
Fragments arranged by size:
3533 1725 649 469 382 313
SapI GCTCTTCn'nnn_
Cuts at: 0 2855 5485 6546 7071
Size: 2855 2630 1061 525
Fragments arranged by size:
2855 2630 1061 525
ScaI AGT'ACT
Cuts at: 0 3517 7071
Size: 3517 3554
SexAI A'CCwGG_T
Cuts at: 0 4987 6012 7058 7071
Size: 4987 1025 1046 13
Fragments arranged by size:
4987 1046 1025 13
SfcI C'TryA_G
Cuts at: 0 625 1572 1796 2225 2467 2603 3573 3726
Size: 625 947 224 429 242 136 970 153
Cuts at: 3726 3828 4453 5023 5638 6028 6743 6803 6876
Size: 102 625 570 615 390 715 60 73
Cuts at: 6876 6947 7071
Size: 71 124
Fragments arranged by size:
970 947 715 625 625 615 570 429
390 242 224 153 136 124 102 73
71 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3155 7071
Size: 3155 3916
SfoI GGC'GCC
Cuts at: 0 3101 6095 7071
Size: 3101 2994 976
Fragments arranged by size:
3101 2994 976
SmaI CCC'GGG
Cuts at: 0 1015 4493 5930 7071
Size: 1015 3478 1437 1141
Fragments arranged by size:
3478 1437 1141 1015
SmlI C'TyrA_G
Cuts at: 0 2371 2991 4840 5826 6165 7026 7071
Size: 2371 620 1849 986 339 861 45
Fragments arranged by size:
2371 1849 986 861 620 339 45
SspI AAT'ATT
Cuts at: 0 1805 7071
Size: 1805 5266
StuI AGG'CCT
Cuts at: 0 2121 4447 6843 7006 7071
Size: 2121 2326 2396 163 65
Fragments arranged by size:
2396 2326 2121 163 65
StyI C'CwwG_G
Cuts at: 0 1128 2087 4292 4421 5117 5174 5609 6788
Size: 1128 959 2205 129 696 57 435 1179
Cuts at: 6788 7071
Size: 283
Fragments arranged by size:
2205 1179 1128 959 696 435 283 129
57
SwaI ATTT'AAAT
Cuts at: 0 6428 7071
Size: 6428 643
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 563 1061 4531 4675 4867 5365 5634
Size: 421 142 498 3470 144 192 498 269
Cuts at: 5634 6408 7071
Size: 774 663
Fragments arranged by size:
3470 774 663 498 498 421 269 192
144 142
TatI w'GTAC_w
Cuts at: 0 190 898 3023 3515 6542 7071
Size: 190 708 2125 492 3027 529
Fragments arranged by size:
3027 2125 708 529 492 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 723 755 4800 4832 5503 5535 5966 5998
Size: 723 32 4045 32 671 32 431 32
Cuts at: 5998 6518 6550 7071
Size: 520 32 521
Fragments arranged by size:
4045 723 671 521 520 431 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4279 5759 6640 7071
Size: 4279 1480 881 431
Fragments arranged by size:
4279 1480 881 431
XbaI T'CTAG_A
Cuts at: 0 6547 7071
Size: 6547 524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 859 1462 1624 1750 2422 3151 3793 5245
Size: 859 603 162 126 672 729 642 1452
Cuts at: 5245 5793 7071
Size: 548 1278
Fragments arranged by size:
1452 1278 859 729 672 642 603 548
162 126
XhoI C'TCGA_G
Cuts at: 0 4840 5826 6165 7071
Size: 4840 986 339 906
Fragments arranged by size:
4840 986 906 339
XmaI C'CCGG_G
Cuts at: 0 1013 4491 5928 7071
Size: 1013 3478 1437 1143
Fragments arranged by size:
3478 1437 1143 1013
XmnI GAAnn'nnTTC
Cuts at: 0 1391 7071
Size: 1391 5680
ZraI GAC'GTC
Cuts at: 0 5151 6585 7071
Size: 5151 1434 486
Fragments arranged by size:
5151 1434 486
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NruI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SalI SanDI SapI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AloI AscI AsiSI AvrII BfrBI
BglII BsaBI BsmI BssHII BssSI BstZ17I EagI EcoRI
FseI FspI FspAI HpaI KpnI MfeI MluI NdeI
NotI NsiI PacI PmeI PsiI PsrI PvuI RsrII
SacII SbfI SgfI SgrAI SnaBI SpeI SphI SrfI