Restriction map for pF1KA0309
 (Linear) (Six-base) MAPSORT of: KIAA0309.seq  Check: 6249  from: 1  to: 9147



KIAA0309.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3102    7611    8025    9147 
   Size:       3102    4509     414    1122

  Fragments arranged by size: 

                4509    3102    1122     414
 
AatII G_ACGT'C

Cuts at:      0    9027    9147 
   Size:       9027     120
 
AccI GT'mk_AC

Cuts at:      0    4363    9147 
   Size:       4363    4784
 
Acc65I G'GTAC_C

Cuts at:      0    4398    4629    9147 
   Size:       4398     231    4518

  Fragments arranged by size: 

                4518    4398     231
 
AclI AA'CG_TT

Cuts at:      0    1992    9147 
   Size:       1992    7155
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    1046    1058    1535    2682    3602    3626    4790 
   Size:        313     733      12     477    1147     920      24    1164

Cuts at:   4790    5237    6155    6785    7388    7432    7556    8999    9147 
   Size:        447     918     630     603      44     124    1443     148

  Fragments arranged by size: 

                1443    1164    1147     920     918     733     630     603
                 477     447     313     148     124      44      24      12
            
 
AfeI AGC'GCT

Cuts at:      0    2203    6397    9147 
   Size:       2203    4194    2750

  Fragments arranged by size: 

                4194    2750    2203
 
AflII C'TTAA_G

Cuts at:      0    5559    9147 
   Size:       5559    3588
 
AflIII A'CryG_T

Cuts at:      0    4053    9147 
   Size:       4053    5094
 
AgeI A'CCGG_T

Cuts at:      0    2661    4395    9147 
   Size:       2661    1734    4752

  Fragments arranged by size: 

                4752    2661    1734
 
AhdI GACnn_n'nnGTC

Cuts at:      0     669    4019    5361    9147 
   Size:        669    3350    1342    3786

  Fragments arranged by size: 

                3786    3350    1342     669
 
AleI CACnn'nnGTG

Cuts at:      0    1960    3565    7786    9147 
   Size:       1960    1605    4221    1361

  Fragments arranged by size: 

                4221    1960    1605    1361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1298    6377    7804    9147 
   Size:       1298    5079    1427    1343

  Fragments arranged by size: 

                5079    1427    1343    1298
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    5153    5185    6499    6531    9147 
   Size:       5153      32    1314      32    2616

  Fragments arranged by size: 

                5153    2616    1314      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1306    1426    1702    3019    4309    4497    4650    4692 
   Size:       1306     120     276    1317    1290     188     153      42

Cuts at:   4692    4818    5851    5873    6844    7222    7336    7435    8074 
   Size:        126    1033      22     971     378     114      99     639

Cuts at:   8074    8143    8173    9147 
   Size:         69      30     974

  Fragments arranged by size: 

                1317    1306    1290    1033     974     971     639     378
                 276     188     153     126     120     114      99      69
                  42      30      22
 
ApaI G_GGCC'C

Cuts at:      0     190     480    1376    1655    2992    3104    3865    4741 
   Size:        190     290     896     279    1337     112     761     876

Cuts at:   4741    4855    5196    7880    8204    8886    9147 
   Size:        114     341    2684     324     682     261

  Fragments arranged by size: 

                2684    1337     896     876     761     682     341     324
                 290     279     261     190     114     112
 
ApoI r'AATT_y

Cuts at:      0    6265    6409    9147 
   Size:       6265     144    2738

  Fragments arranged by size: 

                6265    2738     144
 
AvaI C'yCGr_G

Cuts at:      0    1013    1241    3407    3539    4473    5504    5930    6344 
   Size:       1013     228    2166     132     934    1031     426     414

Cuts at:   6344    6662    7818    7978    8554    9147 
   Size:        318    1156     160     576     593

  Fragments arranged by size: 

                2166    1156    1031    1013     934     593     576     426
                 414     318     228     160     132
 
AvrII C'CTAG_G

Cuts at:      0    6778    9147 
   Size:       6778    2369
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4952    4985    9147 
   Size:       4952      33    4162

  Fragments arranged by size: 

                4952    4162      33
 
BanI G'GyrC_C

Cuts at:      0     127     309     443    1997    2828    2979    3301    3897 
   Size:        127     182     134    1554     831     151     322     596

Cuts at:   3897    3936    4104    4293    4398    4629    4659    4942    5199 
   Size:         39     168     189     105     231      30     283     257

Cuts at:   5199    5409    6586    7326    8094    9147 
   Size:        210    1177     740     768    1053

  Fragments arranged by size: 

                1554    1177    1053     831     768     740     596     322
                 283     257     231     210     189     182     168     151
                 134     127     105      39      30
 
BanII G_rGCy'C

Cuts at:      0     190     320     480    1049    1376    1655    2034    2992 
   Size:        190     130     160     569     327     279     379     958

Cuts at:   2992    3026    3104    3463    3546    3865    4480    4741    4831 
   Size:         34      78     359      83     319     615     261      90

Cuts at:   4831    4855    5196    5498    5511    6452    6663    7418    7880 
   Size:         24     341     302      13     941     211     755     462

Cuts at:   7880    8204    8886    9147 
   Size:        324     682     261

  Fragments arranged by size: 

                 958     941     755     682     615     569     462     379
                 359     341     327     324     319     302     279     261
                 261     211     190     160     130      90      83      78
                  34      24      13
 
BbeI G_GCGC'C

Cuts at:      0    2983    9147 
   Size:       2983    6164
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1882    2310    3995    6643    9147 
   Size:       1882     428    1685    2648    2504

  Fragments arranged by size: 

                2648    2504    1882    1685     428
 
BbvCI CC'TCA_GC

Cuts at:      0     738     953    1187    4386    6326    7340    7425    7544 
   Size:        738     215     234    3199    1940    1014      85     119

Cuts at:   7544    9147 
   Size:       1603

  Fragments arranged by size: 

                3199    1940    1603    1014     738     234     215     119
                  85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    8802    8836    9147 
   Size:       8802      34     311

  Fragments arranged by size: 

                8802     311      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1167    4980    9147 
   Size:       1167    3813    4167

  Fragments arranged by size: 

                4167    3813    1167
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1909    3220    5264    5326    6232    7713    9147 
   Size:       1909    1311    2044      62     906    1481    1434

  Fragments arranged by size: 

                2044    1909    1481    1434    1311     906      62
 
BglII A'GATC_T

Cuts at:      0    7206    9147 
   Size:       7206    1941
 
BlpI GC'TnA_GC

Cuts at:      0     263    1228    6254    6350    9147 
   Size:        263     965    5026      96    2797

  Fragments arranged by size: 

                5026    2797     965     263      96
 
Bme1580I G_kGCm'C

Cuts at:      0     140     190     480    1376    1655    2002    2992    3104 
   Size:        140      50     290     896     279     347     990     112

Cuts at:   3104    3306    3865    4741    4855    5196    6125    7061    7122 
   Size:        202     559     876     114     341     929     936      61

Cuts at:   7122    7199    7880    8204    8886    9147 
   Size:         77     681     324     682     261

  Fragments arranged by size: 

                 990     936     929     896     876     682     681     559
                 347     341     324     290     279     261     202     140
                 114     112      77      61      50
 
BmgBI CAC'GTC

Cuts at:      0    5461    9147 
   Size:       5461    3686
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     348    1938    1983    2992    3043    4684    4831    5541 
   Size:        348    1590      45    1009      51    1641     147     710

Cuts at:   5541    6832    6844    8131    8881    9147 
   Size:       1291      12    1287     750     266

  Fragments arranged by size: 

                1641    1590    1291    1287    1009     750     710     348
                 266     147      51      45      12
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2367    6466    8017    8115    9147 
   Size:       2367    4099    1551      98    1032

  Fragments arranged by size: 

                4099    2367    1551    1032      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     163     567     684     732    1631    1696    2728 
   Size:         95      68     404     117      48     899      65    1032

Cuts at:   2728    2996    4139    4163    4244    4352    4598    4622    4688 
   Size:        268    1143      24      81     108     246      24      66

Cuts at:   4688    4706    4715    4754    5143    6118    6199    6436    6448 
   Size:         18       9      39     389     975      81     237      12

Cuts at:   6448    6779    6983    7121    7168    7432    7480    7494    8048 
   Size:        331     204     138      47     264      48      14     554

Cuts at:   8048    9147 
   Size:       1099

  Fragments arranged by size: 

                1143    1099    1032     975     899     554     404     389
                 331     268     264     246     237     204     138     117
                 108      95      81      81      68      66      65      48
                  48      47      39      24      24      18      14      12
                   9
 
Bpu10I CC'TnA_GC

Cuts at:      0     386     738     953    1187    2719    2936    3020    4160 
   Size:        386     352     215     234    1532     217      84    1140

Cuts at:   4160    4386    5894    6326    7340    7425    7544    9147 
   Size:        226    1508     432    1014      85     119    1603

  Fragments arranged by size: 

                1603    1532    1508    1140    1014     432     386     352
                 234     226     217     215     119      85      84
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     613    2035    3565    4845    5605    6659    7116 
   Size:         71     542    1422    1530    1280     760    1054     457

Cuts at:   7116    9147 
   Size:       2031

  Fragments arranged by size: 

                2031    1530    1422    1280    1054     760     542     457
                  71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    7803    8066    8146    8793    8837    9147 
   Size:       7803     263      80     647      44     310

  Fragments arranged by size: 

                7803     647     310     263      80      44
 
BsaAI yAC'GTr

Cuts at:      0    3663    9147 
   Size:       3663    5484
 
BsaBI GATnn'nnATC

Cuts at:      0    2735    9147 
   Size:       2735    6412
 
BsaHI Gr'CG_yC

Cuts at:      0    1753    2238    2980    5076    7620    9024    9147 
   Size:       1753     485     742    2096    2544    1404     123

  Fragments arranged by size: 

                2544    2096    1753    1404     742     485     123
 
BsaWI w'CCGG_w

Cuts at:      0    1436    2661    4284    4338    4395    9147 
   Size:       1436    1225    1623      54      57    4752

  Fragments arranged by size: 

                4752    1623    1436    1225      57      54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1969    1999    2228    2241    2258    2271    2272    2302 
   Size:       1969      30     229      13      17      13       1      30

Cuts at:   2302    4752    4782    5805    5835    6499    6529    6945    6975 
   Size:       2450      30    1023      30     664      30     416      30

Cuts at:   6975    6981    7011    9147 
   Size:          6      30    2136

  Fragments arranged by size: 

                2450    2136    1969    1023     664     416     229      30
                  30      30      30      30      30      30      17      13
                  13       6       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466     502    1279    1385    1483    1573    2206    2999 
   Size:        466      36     777     106      98      90     633     793

Cuts at:   2999    3644    3705    4256    4259    4268    5989    6154    6349 
   Size:        645      61     551       3       9    1721     165     195

Cuts at:   6349    6445    6460    6523    6547    6550    6553    6899    6950 
   Size:         96      15      63      24       3       3     346      51

Cuts at:   6950    7022    7170    7390    7604    8980    9007    9010    9147 
   Size:         72     148     220     214    1376      27       3     137

  Fragments arranged by size: 

                1721    1376     793     777     645     633     551     466
                 346     220     214     195     165     148     137     106
                  98      96      90      72      63      61      51      36
                  27      24      15       9       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0      42    2002    3032    3441    3804    3864    3909    4083 
   Size:         42    1960    1030     409     363      60      45     174

Cuts at:   4083    4509    4740    4746    4800    4884    4902    5029    5284 
   Size:        426     231       6      54      84      18     127     255

Cuts at:   5284    5342    6553    7276    7919    7953    8074    8204    8219 
   Size:         58    1211     723     643      34     121     130      15

Cuts at:   8219    8512    9147 
   Size:        293     635

  Fragments arranged by size: 

                1960    1211    1030     723     643     635     426     409
                 363     293     255     231     174     130     127     121
                  84      60      58      54      45      42      34      18
                  15       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4831    6242    6936    7256    9147 
   Size:       4831    1411     694     320    1891

  Fragments arranged by size: 

                4831    1891    1411     694     320
 
BsiEI CG_ry'CG

Cuts at:      0    2662    4859    9147 
   Size:       2662    2197    4288

  Fragments arranged by size: 

                4288    2662    2197
 
BsiHKAI G_wGCw'C

Cuts at:      0    3026    3463    5498    5645    6452    7418    7998    9147 
   Size:       3026     437    2035     147     807     966     580    1149

  Fragments arranged by size: 

                3026    2035    1149     966     807     580     437     147
            
 
BsmI GAATG_Cn'

Cuts at:      0    1847    5672    8687    9147 
   Size:       1847    3825    3015     460

  Fragments arranged by size: 

                3825    3015    1847     460
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2229    7982    9147 
   Size:       2229    5753    1165

  Fragments arranged by size: 

                5753    2229    1165
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     190     320     480    1049    1376    1655    2002 
   Size:        140      50     130     160     569     327     279     347

Cuts at:   2002    2034    2992    3026    3104    3306    3463    3546    3865 
   Size:         32     958      34      78     202     157      83     319

Cuts at:   3865    4480    4741    4831    4855    5196    5498    5511    5645 
   Size:        615     261      90      24     341     302      13     134

Cuts at:   5645    6125    6452    6663    7061    7122    7199    7418    7880 
   Size:        480     327     211     398      61      77     219     462

Cuts at:   7880    7998    8204    8886    9147 
   Size:        118     206     682     261

  Fragments arranged by size: 

                 958     682     615     569     480     462     398     347
                 341     327     327     319     302     279     261     261
                 219     211     206     202     160     157     140     134
                 130     118      90      83      78      77      61      50
                  34      32      24      13
 
BspHI T'CATG_A

Cuts at:      0     999    2591    9147 
   Size:        999    1592    6556

  Fragments arranged by size: 

                6556    1592     999
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     629    3102    5723    7611    8025    9147 
   Size:        629    2473    2621    1888     414    1122

  Fragments arranged by size: 

                2621    2473    1888    1122     629     414
 
BsrBI CCG'CTC

Cuts at:      0    4948    9147 
   Size:       4948    4199
 
BsrDI GCAATG_nn'

Cuts at:      0     558     870    1688    5011    7410    7931    9147 
   Size:        558     312     818    3323    2399     521    1216

  Fragments arranged by size: 

                3323    2399    1216     818     558     521     312
 
BsrFI r'CCGG_y

Cuts at:      0    2661    3509    4395    6314    6593    9038    9147 
   Size:       2661     848     886    1919     279    2445     109

  Fragments arranged by size: 

                2661    2445    1919     886     848     279     109
 
BssSI C'ACGA_G

Cuts at:      0     448    6635    8291    9147 
   Size:        448    6187    1656     856

  Fragments arranged by size: 

                6187    1656     856     448
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4189    4942    5792    6820    9147 
   Size:       4189     753     850    1028    2327

  Fragments arranged by size: 

                4189    2327    1028     850     753
 
BstBI TT'CG_AA

Cuts at:      0    7698    9147 
   Size:       7698    1449
 
BstEII G'GTnAC_C

Cuts at:      0    1818    2664    4021    9147 
   Size:       1818     846    1357    5126

  Fragments arranged by size: 

                5126    1818    1357     846
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1801    4215    5447    8212    8715    9147 
   Size:       1801    2414    1232    2765     503     432

  Fragments arranged by size: 

                2765    2414    1801    1232     503     432
 
BstYI r'GATC_y

Cuts at:      0    5728    7206    7229    8169    9147 
   Size:       5728    1478      23     940     978

  Fragments arranged by size: 

                5728    1478     978     940      23
 
Bsu36I CC'TnA_GG

Cuts at:      0     305    1052    1397    7103    7589    8951    8985    9147 
   Size:        305     747     345    5706     486    1362      34     162

  Fragments arranged by size: 

                5706    1362     747     486     345     305     162      34
            
 
BtgI C'CryG_G

Cuts at:      0     521    1220    1259    1376    2726    2974    3359    4076 
   Size:        521     699      39     117    1350     248     385     717

Cuts at:   4076    4964    5700    5885    8708    9147 
   Size:        888     736     185    2823     439

  Fragments arranged by size: 

                2823    1350     888     736     717     699     521     439
                 385     248     185     117      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2314    4283    9147 
   Size:       2314    1969    4864

  Fragments arranged by size: 

                4864    2314    1969
 
BtsI GCAGTG_nn'

Cuts at:      0     370    1320    1638    3151    5042    5609    7903    7996 
   Size:        370     950     318    1513    1891     567    2294      93

Cuts at:   7996    8395    8411    9147 
   Size:        399      16     736

  Fragments arranged by size: 

                2294    1891    1513     950     736     567     399     370
                 318      93      16
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1894    1929    9147 
   Size:       1894      35    7218

  Fragments arranged by size: 

                7218    1894      35
 
DraI TTT'AAA

Cuts at:      0    2010    9147 
   Size:       2010    7137
 
DraIII CAC_nnn'GTG

Cuts at:      0    5640    9147 
   Size:       5640    3507
 
EaeI y'GGCC_r

Cuts at:      0     524    1733    3507    3983    4121    4856    5703    5922 
   Size:        524    1209    1774     476     138     735     847     219

Cuts at:   5922    6023    6155    6312    7163    7666    9147 
   Size:        101     132     157     851     503    1481

  Fragments arranged by size: 

                1774    1481    1209     851     847     735     524     503
                 476     219     157     138     132     101
 
EagI C'GGCC_G

Cuts at:      0    4856    9147 
   Size:       4856    4291
 
EarI CTCTTCn'nnn_

Cuts at:      0     196     289     350     720     738     812     923    1037 
   Size:        196      93      61     370      18      74     111     114

Cuts at:   1037    1127    1145    1163    1382    1511    1550    1601    3581 
   Size:         90      18      18     219     129      39      51    1980

Cuts at:   3581    3654    3696    4053    4286    5998    6131    6140    6203 
   Size:         73      42     357     233    1712     133       9      63

Cuts at:   6203    6458    7475    8467    8689    9147 
   Size:        255    1017     992     222     458

  Fragments arranged by size: 

                1980    1712    1017     992     458     370     357     255
                 233     222     219     196     133     129     114     111
                  93      90      74      73      63      61      51      42
                  39      18      18      18       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    6508    6693    9147 
   Size:       6508     185    2454

  Fragments arranged by size: 

                6508    2454     185
 
EcoICRI GAG'CTC

Cuts at:      0    3024    3461    5496    6450    7416    9147 
   Size:       3024     437    2035     954     966    1731

  Fragments arranged by size: 

                3024    2035    1731     966     954     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     163     313     567     684     732    1046    1058 
   Size:         95      68     150     254     117      48     314      12

Cuts at:   1058    1535    1631    1696    2682    2728    2996    3602    3626 
   Size:        477      96      65     986      46     268     606      24

Cuts at:   3626    4139    4163    4244    4352    4598    4622    4688    4706 
   Size:        513      24      81     108     246      24      66      18

Cuts at:   4706    4715    4754    4790    5143    5237    6118    6155    6199 
   Size:          9      39      36     353      94     881      37      44

Cuts at:   6199    6436    6448    6779    6785    6983    7121    7168    7388 
   Size:        237      12     331       6     198     138      47     220

Cuts at:   7388    7432    7480    7494    7556    8048    8999    9147 
   Size:         44      48      14      62     492     951     148

  Fragments arranged by size: 

                 986     951     881     606     513     492     477     353
                 331     314     268     254     246     237     220     198
                 150     148     138     117     108      96      95      94
                  81      68      66      65      62      48      48      47
                  46      44      44      39      37      36      24      24
                  24      18      14      12      12       9       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1028    1646    2419    3011    5989    9147 
   Size:       1028     618     773     592    2978    3158

  Fragments arranged by size: 

                3158    2978    1028     773     618     592
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     186     967    1372    1651    1933    2989    3016 
   Size:        115      71     781     405     279     282    1056      27

Cuts at:   3016    3101    4582    5192    5193    7472    7594    7684    7877 
   Size:         85    1481     610       1    2279     122      90     193

Cuts at:   7877    8042    8201    8882    8883    8947    9011    9147 
   Size:        165     159     681       1      64      64     136

  Fragments arranged by size: 

                2279    1481    1056     781     681     610     405     282
                 279     193     165     159     136     122     115      90
                  85      71      64      64      27       1       1
 
EcoRV GAT'ATC

Cuts at:      0    6216    9147 
   Size:       6216    2931
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1270    9147 
   Size:       1270    7877
 
FseI GG_CCGG'CC

Cuts at:      0    6318    9147 
   Size:       6318    2829
 
FspI TGC'GCA

Cuts at:      0     430    5542    9082    9147 
   Size:        430    5112    3540      65

  Fragments arranged by size: 

                5112    3540     430      65
 
FspAI rTGC'GCAy

Cuts at:      0     430    5542    9147 
   Size:        430    5112    3605

  Fragments arranged by size: 

                5112    3605     430
 
HaeII r_GCGC'y

Cuts at:      0     396    2154    2205    2983    4879    6399    9092    9147 
   Size:        396    1758      51     778    1896    1520    2693      55

  Fragments arranged by size: 

                2693    1896    1758    1520     778     396      55      51
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     237     269     655     687    1519    1551    1878    1910 
   Size:        237      32     386      32     832      32     327      32

Cuts at:   1910    2228    2241    2260    2272    2273    2304    4005    4037 
   Size:        318      13      19      12       1      31    1701      32

Cuts at:   4037    4680    4712    4752    4784    5347    5379    5630    5662 
   Size:        643      32      40      32     563      32     251      32

Cuts at:   5662    6325    6357    7459    7491    8323    8355    9147 
   Size:        663      32    1102      32     832      32     792

  Fragments arranged by size: 

                1701    1102     832     832     792     663     643     563
                 386     327     318     251     237      40      32      32
                  32      32      32      32      32      32      32      32
                  32      32      31      19      13      12       1
 
HincII GTy'rAC

Cuts at:      0    2524    2913    3500    3875    9147 
   Size:       2524     389     587     375    5272

  Fragments arranged by size: 

                5272    2524     587     389     375
 
HindIII A'AGCT_T

Cuts at:      0    1837    9147 
   Size:       1837    7310
 
HpaI GTT'AAC

Cuts at:      0    3500    9147 
   Size:       3500    5647
 
KasI G'GCGC_C

Cuts at:      0    2979    9147 
   Size:       2979    6168
 
KpnI G_GTAC'C

Cuts at:      0    4402    4633    9147 
   Size:       4402     231    4514

  Fragments arranged by size: 

                4514    4402     231
 
MfeI C'AATT_G

Cuts at:      0    5482    9147 
   Size:       5482    3665
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     941    1040    1178    4432    4615    4705    5196 
   Size:        379     562      99     138    3254     183      90     491

Cuts at:   5196    8011    8329    8747    9147 
   Size:       2815     318     418     400

  Fragments arranged by size: 

                3254    2815     562     491     418     400     379     318
                 183     138      99      90
 
MscI TGG'CCA

Cuts at:      0     526    1735    3985    4123    5705    5924    6025    6157 
   Size:        526    1209    2250     138    1582     219     101     132

Cuts at:   6157    7165    9147 
   Size:       1008    1982

  Fragments arranged by size: 

                2250    1982    1582    1209    1008     526     219     138
                 132     101
 
MslI CAynn'nnrTG

Cuts at:      0    1004    1118    1960    2611    3565    5656    7786    8713 
   Size:       1004     114     842     651     954    2091    2130     927

Cuts at:   8713    9147 
   Size:        434

  Fragments arranged by size: 

                2130    2091    1004     954     927     842     651     434
                 114
 
MspA1I CmG'CkG

Cuts at:      0     459     742     957    1186    1702    2504    2619    2748 
   Size:        459     283     215     229     516     802     115     129

Cuts at:   2748    3342    3475    3913    5145    5361    5909    6381    6740 
   Size:        594     133     438    1232     216     548     472     359

Cuts at:   6740    7543    7767    9147 
   Size:        803     224    1380

  Fragments arranged by size: 

                1380    1232     803     802     594     548     516     472
                 459     438     359     283     229     224     216     215
                 133     129     115
 
NaeI GCC'GGC

Cuts at:      0    3511    6316    9040    9147 
   Size:       3511    2805    2724     107

  Fragments arranged by size: 

                3511    2805    2724     107
 
NarI GG'CG_CC

Cuts at:      0    2980    9147 
   Size:       2980    6167
 
NcoI C'CATG_G

Cuts at:      0     521    1220    1259    3359    4964    5700    5885    8708 
   Size:        521     699      39    2100    1605     736     185    2823

Cuts at:   8708    9147 
   Size:        439

  Fragments arranged by size: 

                2823    2100    1605     736     699     521     439     185
                  39
 
NgoMIV G'CCGG_C

Cuts at:      0    3509    6314    9038    9147 
   Size:       3509    2805    2724     109

  Fragments arranged by size: 

                3509    2805    2724     109
 
NruI TCG'CGA

Cuts at:      0    2333    9147 
   Size:       2333    6814
 
NspI r_CATG'y

Cuts at:      0    2497    4057    4091    5431    5541    7845    9147 
   Size:       2497    1560      34    1340     110    2304    1302

  Fragments arranged by size: 

                2497    2304    1560    1340    1302     110      34
 
PasI CC'CwG_GG

Cuts at:      0    2985    3679    4578    4848    8143    8308    8589    8943 
   Size:       2985     694     899     270    3295     165     281     354

Cuts at:   8943    9147 
   Size:        204

  Fragments arranged by size: 

                3295    2985     899     694     354     281     270     204
                 165
 
PciI A'CATG_T

Cuts at:      0    4053    9147 
   Size:       4053    5094
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1306    5300    7128    8143    8893    9147 
   Size:       1306    3994    1828    1015     750     254

  Fragments arranged by size: 

                3994    1828    1306    1015     750     254
 
PfoI T'CCnGG_A

Cuts at:      0    1784    3918    4368    5222    5474    6413    8177    9147 
   Size:       1784    2134     450     854     252     939    1764     970

  Fragments arranged by size: 

                2134    1784    1764     970     939     854     450     252
            
 
PmlI CAC'GTG

Cuts at:      0    3663    9147 
   Size:       3663    5484
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    6499    6531    9147 
   Size:       6499      32    2616

  Fragments arranged by size: 

                6499    2616      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1933    3016    4582    7472    7594    9011    9147 
   Size:       1933    1083    1566    2890     122    1417     136

  Fragments arranged by size: 

                2890    1933    1566    1417    1083     136     122
 
PshAI GACnn'nnGTC

Cuts at:      0    2759    5218    5909    6637    9147 
   Size:       2759    2459     691     728    2510

  Fragments arranged by size: 

                2759    2510    2459     728     691
 
PspOMI G'GGCC_C

Cuts at:      0     186     476    1372    1651    2988    3100    3861    4737 
   Size:        186     290     896     279    1337     112     761     876

Cuts at:   4737    4851    5192    7876    8200    8882    9147 
   Size:        114     341    2684     324     682     265

  Fragments arranged by size: 

                2684    1337     896     876     761     682     341     324
                 290     279     265     186     114     112
 
PspXI vC'TCGA_Gb

Cuts at:      0    3539    9147 
   Size:       3539    5608
 
PstI C_TGCA'G

Cuts at:      0    1698    2120    2509    2927    3158    6745    8328    9147 
   Size:       1698     422     389     418     231    3587    1583     819

  Fragments arranged by size: 

                3587    1698    1583     819     422     418     389     231
            
 
PvuII CAG'CTG

Cuts at:      0     742     957    1186    1702    2619    3475    5361    6381 
   Size:        742     215     229     516     917     856    1886    1020

Cuts at:   6381    7543    9147 
   Size:       1162    1604

  Fragments arranged by size: 

                1886    1604    1162    1020     917     856     742     516
                 229     215
 
SacI G_AGCT'C

Cuts at:      0    3026    3463    5498    6452    7418    9147 
   Size:       3026     437    2035     954     966    1729

  Fragments arranged by size: 

                3026    2035    1729     966     954     437
 
SanDI GG'GwC_CC

Cuts at:      0    1933    4582    9011    9147 
   Size:       1933    2649    4429     136

  Fragments arranged by size: 

                4429    2649    1933     136
 
SapI GCTCTTCn'nnn_

Cuts at:      0     196     720     738     923    1037    1382    1601    6458 
   Size:        196     524      18     185     114     345     219    4857

Cuts at:   6458    7475    9147 
   Size:       1017    1672

  Fragments arranged by size: 

                4857    1672    1017     524     345     219     196     185
                 114      18
 
ScaI AGT'ACT

Cuts at:      0     658    1588    9147 
   Size:        658     930    7559

  Fragments arranged by size: 

                7559     930     658
 
SexAI A'CCwGG_T

Cuts at:      0    4017    9147 
   Size:       4017    5130
 
SfcI C'TryA_G

Cuts at:      0    1178    1694    2116    2505    2923    3154    3743    6741 
   Size:       1178     516     422     389     418     231     589    2998

Cuts at:   6741    8324    8926    9147 
   Size:       1583     602     221

  Fragments arranged by size: 

                2998    1583    1178     602     589     516     422     418
                 389     231     221
 
SfoI GGC'GCC

Cuts at:      0    2981    9147 
   Size:       2981    6166
 
SgrAI Cr'CCGG_yG

Cuts at:      0    6593    9147 
   Size:       6593    2554
 
SmaI CCC'GGG

Cuts at:      0    5506    5932    6346    6664    7820    9147 
   Size:       5506     426     414     318    1156    1327

  Fragments arranged by size: 

                5506    1327    1156     426     414     318
 
SmlI C'TyrA_G

Cuts at:      0      86     592    1241    2050    3539    3580    4824    5559 
   Size:         86     506     649     809    1489      41    1244     735

Cuts at:   5559    5620    6674    7095    8554    9147 
   Size:         61    1054     421    1459     593

  Fragments arranged by size: 

                1489    1459    1244    1054     809     735     649     593
                 506     421      86      61      41
 
SpeI A'CTAG_T

Cuts at:      0    7807    9147 
   Size:       7807    1340
 
SphI G_CATG'C

Cuts at:      0    4091    5431    9147 
   Size:       4091    1340    3716

  Fragments arranged by size: 

                4091    3716    1340
 
SrfI GCCC'GGGC

Cuts at:      0    6664    9147 
   Size:       6664    2483
 
StuI AGG'CCT

Cuts at:      0    2131    2433    3573    5755    6421    6783    7101    9147 
   Size:       2131     302    1140    2182     666     362     318    2046

  Fragments arranged by size: 

                2182    2131    2046    1140     666     362     318     302
            
 
StyI C'CwwG_G

Cuts at:      0     521     527    1108    1220    1259    1718    3359    3932 
   Size:        521       6     581     112      39     459    1641     573

Cuts at:   3932    4871    4964    4988    5446    5700    5885    6236    6503 
   Size:        939      93      24     458     254     185     351     267

Cuts at:   6503    6778    7088    7286    8595    8708    9060    9147 
   Size:        275     310     198    1309     113     352      87

  Fragments arranged by size: 

                1641    1309     939     581     573     521     459     458
                 352     351     310     275     267     254     198     185
                 113     112      93      87      39      24       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3573    4536    5453    5560    5668    6244    6604    7290 
   Size:       3573     963     917     107     108     576     360     686

Cuts at:   7290    8384    8478    9147 
   Size:       1094      94     669

  Fragments arranged by size: 

                3573    1094     963     917     686     669     576     360
                 108     107      94
 
TatI w'GTAC_w

Cuts at:      0     656    1586    5331    9147 
   Size:        656     930    3745    3816

  Fragments arranged by size: 

                3816    3745     930     656
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     334     366     393     425    3652    3684    3780    3809 
   Size:        334      32      27      32    3227      32      96      29

Cuts at:   3809    3812    3841    3890    3922    5805    5837    6945    6977 
   Size:          3      29      49      32    1883      32    1108      32

Cuts at:   6977    9147 
   Size:       2170

  Fragments arranged by size: 

                3227    2170    1883    1108     334      96      49      32
                  32      32      32      32      32      29      29      27
                   3
 
Tth111I GACn'n_nGTC

Cuts at:      0    4602    4650    4692    8137    9147 
   Size:       4602      48      42    3445    1010

  Fragments arranged by size: 

                4602    3445    1010      48      42
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138    3040    3525    3561    6411    7284    7635    9147 
   Size:        138    2902     485      36    2850     873     351    1512

  Fragments arranged by size: 

                2902    2850    1512     873     485     351     138      36
            
 
XhoI C'TCGA_G

Cuts at:      0    1241    3539    8554    9147 
   Size:       1241    2298    5015     593

  Fragments arranged by size: 

                5015    2298    1241     593
 
XmaI C'CCGG_G

Cuts at:      0    5504    5930    6344    6662    7818    9147 
   Size:       5504     426     414     318    1156    1329

  Fragments arranged by size: 

                5504    1329    1156     426     414     318
 
XmnI GAAnn'nnTTC

Cuts at:      0     513    9147 
   Size:        513    8634
 
ZraI GAC'GTC

Cuts at:      0    9025    9147 
   Size:       9025     122
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NruI     NspI     PasI     PciI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII
     SacI    SanDI     SapI     ScaI    SexAI     SfcI     SfoI    SgrAI
     SmaI     SmlI     SpeI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    ApaLI     AscI     AseI    AsiSI    BamHI     BclI    BfrBI     BmtI
    BsiWI    BspEI    BsrGI   BssHII  BstZ17I     ClaI     DrdI    EcoRI
     MluI     NdeI     NheI     NotI     NsiI     PacI     PmeI     PsiI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     SfiI     SgfI
    SnaBI     SspI     SwaI     XbaI
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