Restriction map for
pF1KA0305
(Linear) (Six-base) MAPSORT of: KIAA0305.seq Check: 9341 from: 1 to: 4617
KIAA0305.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 2526 3709 3882 4127 4617
Size: 2526 1183 173 245 490
Fragments arranged by size:
2526 1183 490 245 173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 314 485 1067 1315 1609 2156 2708
Size: 116 198 171 582 248 294 547 552
Cuts at: 2708 2945 3327 3974 4187 4502 4617
Size: 237 382 647 213 315 115
Fragments arranged by size:
647 582 552 547 382 315 294 248
237 213 198 171 116 115
AflII C'TTAA_G
Cuts at: 0 1051 2461 4617
Size: 1051 1410 2156
Fragments arranged by size:
2156 1410 1051
AflIII A'CryG_T
Cuts at: 0 728 1092 4105 4617
Size: 728 364 3013 512
Fragments arranged by size:
3013 728 512 364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2503 2535 4617
Size: 2503 32 2082
Fragments arranged by size:
2503 2082 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1909 2923 3106 4480 4617
Size: 1909 1014 183 1374 137
Fragments arranged by size:
1909 1374 1014 183 137
ApoI r'AATT_y
Cuts at: 0 675 951 966 2275 3214 3524 3810 3896
Size: 675 276 15 1309 939 310 286 86
Cuts at: 3896 4465 4617
Size: 569 152
Fragments arranged by size:
1309 939 675 569 310 286 276 152
86 15
AseI AT'TA_AT
Cuts at: 0 1162 1339 1499 1775 4617
Size: 1162 177 160 276 2842
Fragments arranged by size:
2842 1162 276 177 160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2036 2069 4617
Size: 2036 33 2548
Fragments arranged by size:
2548 2036 33
BanII G_rGCy'C
Cuts at: 0 3978 4617
Size: 3978 639
BbsI GAAGACnn'nnnn_
Cuts at: 0 1037 1123 1361 1466 2696 3031 4617
Size: 1037 86 238 105 1230 335 1586
Fragments arranged by size:
1586 1230 1037 335 238 105 86
BbvCI CC'TCA_GC
Cuts at: 0 1196 4499 4617
Size: 1196 3303 118
Fragments arranged by size:
3303 1196 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2285 2319 4617
Size: 2285 34 2298
Fragments arranged by size:
2298 2285 34
BfrBI ATG'CAT
Cuts at: 0 103 4617
Size: 103 4514
BglII A'GATC_T
Cuts at: 0 692 4617
Size: 692 3925
Bme1580I G_kGCm'C
Cuts at: 0 4390 4617
Size: 4390 227
BmtI G_CTAG'C
Cuts at: 0 4075 4617
Size: 4075 542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3107 4031 4617
Size: 3107 924 586
Fragments arranged by size:
3107 924 586
Bpu10I CC'TnA_GC
Cuts at: 0 1196 1946 2420 4499 4617
Size: 1196 750 474 2079 118
Fragments arranged by size:
2079 1196 750 474 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1424 3562 4617
Size: 238 1186 2138 1055
Fragments arranged by size:
2138 1186 1055 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 4045 4617
Size: 4045 572
BsaAI yAC'GTr
Cuts at: 0 4143 4617
Size: 4143 474
BsaBI GATnn'nnATC
Cuts at: 0 3869 4617
Size: 3869 748
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2492 3608 4617
Size: 2492 1116 1009
Fragments arranged by size:
2492 1116 1009
BseYI C'CCAG_C
Cuts at: 0 2032 4154 4617
Size: 2032 2122 463
Fragments arranged by size:
2122 2032 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 3614 4617
Size: 1007 2607 1003
Fragments arranged by size:
2607 1007 1003
BsiHKAI G_wGCw'C
Cuts at: 0 3978 4530 4617
Size: 3978 552 87
Fragments arranged by size:
3978 552 87
Bsp1286I G_dGCh'C
Cuts at: 0 3978 4390 4530 4617
Size: 3978 412 140 87
Fragments arranged by size:
3978 412 140 87
BspHI T'CATG_A
Cuts at: 0 3129 4617
Size: 3129 1488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 916 4617
Size: 916 3701
BsrDI GCAATG_nn'
Cuts at: 0 160 1521 2301 4617
Size: 160 1361 780 2316
Fragments arranged by size:
2316 1361 780 160
BsrGI T'GTAC_A
Cuts at: 0 93 4617
Size: 93 4524
BssSI C'ACGA_G
Cuts at: 0 3202 4617
Size: 3202 1415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3613 4617
Size: 3613 1004
BstBI TT'CG_AA
Cuts at: 0 3696 4617
Size: 3696 921
BstEII G'GTnAC_C
Cuts at: 0 1865 2873 4617
Size: 1865 1008 1744
Fragments arranged by size:
1865 1744 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 805 4617
Size: 805 3812
BstYI r'GATC_y
Cuts at: 0 692 1368 2143 4477 4617
Size: 692 676 775 2334 140
Fragments arranged by size:
2334 775 692 676 140
BstZ17I GTA'TAC
Cuts at: 0 2527 3710 3883 4617
Size: 2527 1183 173 734
Fragments arranged by size:
2527 1183 734 173
Bsu36I CC'TnA_GG
Cuts at: 0 2504 3332 4617
Size: 2504 828 1285
Fragments arranged by size:
2504 1285 828
BtgI C'CryG_G
Cuts at: 0 3775 4364 4545 4617
Size: 3775 589 181 72
Fragments arranged by size:
3775 589 181 72
BtsI GCAGTG_nn'
Cuts at: 0 117 1235 4617
Size: 117 1118 3382
Fragments arranged by size:
3382 1118 117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3941 3976 4617
Size: 3941 35 641
Fragments arranged by size:
3941 641 35
DraI TTT'AAA
Cuts at: 0 15 680 878 4098 4617
Size: 15 665 198 3220 519
Fragments arranged by size:
3220 665 519 198 15
DraIII CAC_nnn'GTG
Cuts at: 0 1183 4617
Size: 1183 3434
EaeI y'GGCC_r
Cuts at: 0 3927 4617
Size: 3927 690
EarI CTCTTCn'nnn_
Cuts at: 0 878 1086 2596 4617
Size: 878 208 1510 2021
Fragments arranged by size:
2021 1510 878 208
EcoICRI GAG'CTC
Cuts at: 0 3976 4617
Size: 3976 641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 314 485 1067 1315 1609 2156 2708
Size: 116 198 171 582 248 294 547 552
Cuts at: 2708 2945 3107 3327 3974 4031 4187 4502 4617
Size: 237 162 220 647 57 156 315 115
Fragments arranged by size:
647 582 552 547 315 294 248 237
220 198 171 162 156 116 115 57
EcoO109I rG'GnC_Cy
Cuts at: 0 1989 4553 4617
Size: 1989 2564 64
Fragments arranged by size:
2564 1989 64
EcoRI G'AATT_C
Cuts at: 0 3524 4617
Size: 3524 1093
EcoRV GAT'ATC
Cuts at: 0 4149 4617
Size: 4149 468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1465 3357 3527 4507 4617
Size: 1465 1892 170 980 110
Fragments arranged by size:
1892 1465 980 170 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 684 716 1099 1131 1657 1689
Size: 448 32 204 32 383 32 526 32
Cuts at: 1689 1878 1910 2427 2459 3338 3370 3478 3510
Size: 189 32 517 32 879 32 108 32
Cuts at: 3510 4195 4227 4617
Size: 685 32 390
Fragments arranged by size:
879 685 526 517 448 390 383 204
189 108 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2958 3690 4617
Size: 2958 732 927
Fragments arranged by size:
2958 927 732
HindIII A'AGCT_T
Cuts at: 0 3538 4617
Size: 3538 1079
HpaI GTT'AAC
Cuts at: 0 2958 4617
Size: 2958 1659
MfeI C'AATT_G
Cuts at: 0 713 1951 4617
Size: 713 1238 2666
Fragments arranged by size:
2666 1238 713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1911 2470 4583 4617
Size: 1911 559 2113 34
Fragments arranged by size:
2113 1911 559 34
MscI TGG'CCA
Cuts at: 0 3929 4617
Size: 3929 688
MslI CAynn'nnrTG
Cuts at: 0 542 2477 3673 4456 4617
Size: 542 1935 1196 783 161
Fragments arranged by size:
1935 1196 783 542 161
MspA1I CmG'CkG
Cuts at: 0 22 2083 4617
Size: 22 2061 2534
Fragments arranged by size:
2534 2061 22
NcoI C'CATG_G
Cuts at: 0 4364 4545 4617
Size: 4364 181 72
Fragments arranged by size:
4364 181 72
NdeI CA'TA_TG
Cuts at: 0 105 3743 4617
Size: 105 3638 874
Fragments arranged by size:
3638 874 105
NheI G'CTAG_C
Cuts at: 0 4071 4617
Size: 4071 546
NsiI A_TGCA'T
Cuts at: 0 105 4617
Size: 105 4512
NspI r_CATG'y
Cuts at: 0 732 1096 2315 3829 4109 4617
Size: 732 364 1219 1514 280 508
Fragments arranged by size:
1514 1219 732 508 364 280
PasI CC'CwG_GG
Cuts at: 0 3551 4617
Size: 3551 1066
PciI A'CATG_T
Cuts at: 0 728 1092 4105 4617
Size: 728 364 3013 512
Fragments arranged by size:
3013 728 512 364
PpuMI rG'GwC_Cy
Cuts at: 0 1989 4553 4617
Size: 1989 2564 64
Fragments arranged by size:
2564 1989 64
PshAI GACnn'nnGTC
Cuts at: 0 2696 4617
Size: 2696 1921
PsiI TTA'TAA
Cuts at: 0 1012 4617
Size: 1012 3605
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2667 2699 4617
Size: 2667 32 1918
Fragments arranged by size:
2667 1918 32
PstI C_TGCA'G
Cuts at: 0 913 4617
Size: 913 3704
PvuII CAG'CTG
Cuts at: 0 22 2083 4617
Size: 22 2061 2534
Fragments arranged by size:
2534 2061 22
SacI G_AGCT'C
Cuts at: 0 3978 4617
Size: 3978 639
ScaI AGT'ACT
Cuts at: 0 3806 4617
Size: 3806 811
SexAI A'CCwGG_T
Cuts at: 0 2568 4617
Size: 2568 2049
SfcI C'TryA_G
Cuts at: 0 909 1424 2069 2078 2756 3107 4617
Size: 909 515 645 9 678 351 1510
Fragments arranged by size:
1510 909 678 645 515 351 9
SmlI C'TyrA_G
Cuts at: 0 253 1051 1439 2461 3541 4617
Size: 253 798 388 1022 1080 1076
Fragments arranged by size:
1080 1076 1022 798 388 253
SspI AAT'ATT
Cuts at: 0 1551 3241 3408 4617
Size: 1551 1690 167 1209
Fragments arranged by size:
1690 1551 1209 167
StyI C'CwwG_G
Cuts at: 0 4364 4545 4617
Size: 4364 181 72
Fragments arranged by size:
4364 181 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3580 4617
Size: 3580 1037
TatI w'GTAC_w
Cuts at: 0 93 2485 3785 3804 3853 4617
Size: 93 2392 1300 19 49 764
Fragments arranged by size:
2392 1300 764 93 49 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3915 3947 4617
Size: 3915 32 670
Fragments arranged by size:
3915 670 32
XbaI T'CTAG_A
Cuts at: 0 2367 4617
Size: 2367 2250
XmnI GAAnn'nnTTC
Cuts at: 0 3164 4617
Size: 3164 1453
Enzymes that do cut:
AccI AcuI AflII AflIII AloI AlwNI ApoI AseI
BaeI BanII BbsI BbvCI BcgI BfrBI BglII Bme1580I
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI CspCI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PasI PciI PpuMI PshAI PsiI
PsrI PstI PvuII SacI ScaI SexAI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI
BanI BbeI BciVI BclI BglI BlpI BmgBI BmrI
BplI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BssHII BtgZI ClaI DrdI EagI
EciI EcoNI FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI PacI PflMI
PfoI PmeI PmlI PpiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XcmI XhoI XmaI ZraI