Restriction map for
pF1KA0296
(Linear) (Six-base) MAPSORT of: KIAA0296.seq Check: 9328 from: 1 to: 5487
KIAA0296.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2977 3997 4154 4375 4817 5293 5487
Size: 2977 1020 157 221 442 476 194
Fragments arranged by size:
2977 1020 476 442 221 194 157
AatII G_ACGT'C
Cuts at: 0 4999 5487
Size: 4999 488
AccI GT'mk_AC
Cuts at: 0 1199 5487
Size: 1199 4288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1914 2117 2177 2222 3591 3605 4042
Size: 862 1052 203 60 45 1369 14 437
Cuts at: 4042 5487
Size: 1445
Fragments arranged by size:
1445 1369 1052 862 437 203 60 45
14
AfeI AGC'GCT
Cuts at: 0 2849 5487
Size: 2849 2638
AflII C'TTAA_G
Cuts at: 0 5155 5487
Size: 5155 332
AleI CACnn'nnGTG
Cuts at: 0 2368 2876 4087 4499 5487
Size: 2368 508 1211 412 988
Fragments arranged by size:
2368 1211 988 508 412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1546 3382 4105 4835 5487
Size: 1546 1836 723 730 652
Fragments arranged by size:
1836 1546 730 723 652
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4418 4450 5487
Size: 4418 32 1037
Fragments arranged by size:
4418 1037 32
AlwNI CAG_nnn'CTG
Cuts at: 0 538 639 1050 2395 2522 2779 3107 4038
Size: 538 101 411 1345 127 257 328 931
Cuts at: 4038 4797 5207 5487
Size: 759 410 280
Fragments arranged by size:
1345 931 759 538 411 410 328 280
257 127 101
ApaI G_GGCC'C
Cuts at: 0 3338 3380 5487
Size: 3338 42 2107
Fragments arranged by size:
3338 2107 42
ApaLI G'TGCA_C
Cuts at: 0 1040 5487
Size: 1040 4447
ApoI r'AATT_y
Cuts at: 0 3036 5487
Size: 3036 2451
AvaI C'yCGr_G
Cuts at: 0 178 917 2087 3878 4823 5267 5477 5487
Size: 178 739 1170 1791 945 444 210 10
Fragments arranged by size:
1791 1170 945 739 444 210 178 10
AvrII C'CTAG_G
Cuts at: 0 1840 5487
Size: 1840 3647
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 705 738 5487
Size: 705 33 4749
Fragments arranged by size:
4749 705 33
BanI G'GyrC_C
Cuts at: 0 74 1582 2038 2699 3085 3862 4142 4839
Size: 74 1508 456 661 386 777 280 697
Cuts at: 4839 5487
Size: 648
Fragments arranged by size:
1508 777 697 661 648 456 386 280
74
BanII G_rGCy'C
Cuts at: 0 68 588 680 1070 1266 2737 3326 3338
Size: 68 520 92 390 196 1471 589 12
Cuts at: 3338 3380 3479 3551 4327 5477 5487
Size: 42 99 72 776 1150 10
Fragments arranged by size:
1471 1150 776 589 520 390 196 99
92 72 68 42 12 10
BbeI G_GCGC'C
Cuts at: 0 2703 5487
Size: 2703 2784
BbsI GAAGACnn'nnnn_
Cuts at: 0 897 1760 2105 2384 5487
Size: 897 863 345 279 3103
Fragments arranged by size:
3103 897 863 345 279
BbvCI CC'TCA_GC
Cuts at: 0 698 1577 2628 5009 5487
Size: 698 879 1051 2381 478
Fragments arranged by size:
2381 1051 879 698 478
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4655 4689 5487
Size: 4655 34 798
Fragments arranged by size:
4655 798 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1886 2657 3191 3689 3932 4193 4202 4219
Size: 1886 771 534 498 243 261 9 17
Cuts at: 4219 4607 4628 4927 5066 5487
Size: 388 21 299 139 421
Fragments arranged by size:
1886 771 534 498 421 388 299 261
243 139 21 17 9
BglII A'GATC_T
Cuts at: 0 492 5487
Size: 492 4995
BlpI GC'TnA_GC
Cuts at: 0 1069 2509 3140 4268 5487
Size: 1069 1440 631 1128 1219
Fragments arranged by size:
1440 1219 1128 1069 631
Bme1580I G_kGCm'C
Cuts at: 0 1007 1044 1587 2873 3338 3380 3865 5413
Size: 1007 37 543 1286 465 42 485 1548
Cuts at: 5413 5487
Size: 74
Fragments arranged by size:
1548 1286 1007 543 485 465 74 42
37
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 861 1031 1615 2279 2375 4420 4892
Size: 678 183 170 584 664 96 2045 472
Cuts at: 4892 5034 5487
Size: 142 453
Fragments arranged by size:
2045 678 664 584 472 453 183 170
142 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 2589 2643 3121 3253 5487
Size: 103 2486 54 478 132 2234
Fragments arranged by size:
2486 2234 478 132 103 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1108 1123 1487 2134 2938 3068 3248
Size: 334 774 15 364 647 804 130 180
Cuts at: 3248 3439 3502 3577 5260 5487
Size: 191 63 75 1683 227
Fragments arranged by size:
1683 804 774 647 364 334 227 191
180 130 75 63 15
Bpu10I CC'TnA_GC
Cuts at: 0 698 1577 2628 5009 5487
Size: 698 879 1051 2381 478
Fragments arranged by size:
2381 1051 879 698 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1582 2242 3056 3718 5487
Size: 253 1329 660 814 662 1769
Fragments arranged by size:
1769 1329 814 662 660 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 1333 1816 2227 3514 4243 5487
Size: 1333 483 411 1287 729 1244
Fragments arranged by size:
1333 1287 1244 729 483 411
BsaAI yAC'GTr
Cuts at: 0 485 1530 5487
Size: 485 1045 3957
Fragments arranged by size:
3957 1045 485
BsaHI Gr'CG_yC
Cuts at: 0 2700 4742 4996 5487
Size: 2700 2042 254 491
Fragments arranged by size:
2700 2042 491 254
BsaWI w'CCGG_w
Cuts at: 0 5374 5487
Size: 5374 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 276 306 697 727 4418 4448 4949 4979
Size: 276 30 391 30 3691 30 501 30
Cuts at: 4979 5101 5131 5487
Size: 122 30 356
Fragments arranged by size:
3691 501 391 356 276 122 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 581 715 718 991 2400 2706 2755
Size: 130 451 134 3 273 1409 306 49
Cuts at: 2755 2758 3436 3532 3553 4174 4474 4581 4870
Size: 3 678 96 21 621 300 107 289
Cuts at: 4870 5368 5416 5481 5487
Size: 498 48 65 6
Fragments arranged by size:
1409 678 621 498 451 306 300 289
273 134 130 107 96 65 49 48
21 6 3 3
BseYI C'CCAG_C
Cuts at: 0 52 407 557 603 786 971 1156 1588
Size: 52 355 150 46 183 185 185 432
Cuts at: 1588 1698 2449 2968 3407 4430 4544 5487
Size: 110 751 519 439 1023 114 943
Fragments arranged by size:
1023 943 751 519 439 432 355 185
185 183 150 114 110 52 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1419 1583 2765 2879 4024 4394 5019
Size: 569 850 164 1182 114 1145 370 625
Cuts at: 5019 5350 5487
Size: 331 137
Fragments arranged by size:
1182 1145 850 625 569 370 331 164
137 114
BsiHKAI G_wGCw'C
Cuts at: 0 68 1044 1070 1483 2551 5119 5487
Size: 68 976 26 413 1068 2568 368
Fragments arranged by size:
2568 1068 976 413 368 68 26
BsmI GAATG_Cn'
Cuts at: 0 2481 5487
Size: 2481 3006
BsmBI CGTCTCn'nnnn_
Cuts at: 0 352 3958 5487
Size: 352 3606 1529
Fragments arranged by size:
3606 1529 352
Bsp1286I G_dGCh'C
Cuts at: 0 68 588 680 1007 1044 1070 1266 1483
Size: 68 520 92 327 37 26 196 217
Cuts at: 1483 1587 2551 2737 2873 3326 3338 3380 3479
Size: 104 964 186 136 453 12 42 99
Cuts at: 3479 3551 3865 4327 5119 5413 5477 5487
Size: 72 314 462 792 294 64 10
Fragments arranged by size:
964 792 520 462 453 327 314 294
217 196 186 136 104 99 92 72
68 64 42 37 26 12 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2977 3343 3360 3997 4154 4375 4817 5153
Size: 2977 366 17 637 157 221 442 336
Cuts at: 5153 5293 5487
Size: 140 194
Fragments arranged by size:
2977 637 442 366 336 221 194 157
140 17
BsrBI CCG'CTC
Cuts at: 0 708 2261 2458 3600 4140 5022 5487
Size: 708 1553 197 1142 540 882 465
Fragments arranged by size:
1553 1142 882 708 540 465 197
BsrDI GCAATG_nn'
Cuts at: 0 1539 5487
Size: 1539 3948
BsrFI r'CCGG_y
Cuts at: 0 1112 1991 2696 3643 3662 3679 3743 3931
Size: 1112 879 705 947 19 17 64 188
Cuts at: 3931 3950 4395 5368 5487
Size: 19 445 973 119
Fragments arranged by size:
1112 973 947 879 705 445 188 119
64 19 19 17
BssSI C'ACGA_G
Cuts at: 0 208 3961 5487
Size: 208 3753 1526
Fragments arranged by size:
3753 1526 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2779 2992 5042 5487
Size: 2779 213 2050 445
Fragments arranged by size:
2779 2050 445 213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 214 261 391 1377 1465 1512 2067 2500
Size: 214 47 130 986 88 47 555 433
Cuts at: 2500 2614 2976 3003 3226 4014 4261 4367 4516
Size: 114 362 27 223 788 247 106 149
Cuts at: 4516 5487
Size: 971
Fragments arranged by size:
986 971 788 555 433 362 247 223
214 149 130 114 106 88 47 47
27
BstYI r'GATC_y
Cuts at: 0 492 3215 3759 4129 4866 5487
Size: 492 2723 544 370 737 621
Fragments arranged by size:
2723 737 621 544 492 370
Bsu36I CC'TnA_GG
Cuts at: 0 296 539 5487
Size: 296 243 4948
Fragments arranged by size:
4948 296 243
BtgI C'CryG_G
Cuts at: 0 260 1685 1964 2996 3186 5000 5247 5487
Size: 260 1425 279 1032 190 1814 247 240
Fragments arranged by size:
1814 1425 1032 279 260 247 240 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1432 5487
Size: 1432 4055
BtsI GCAGTG_nn'
Cuts at: 0 160 891 1407 2794 3366 3913 5193 5487
Size: 160 731 516 1387 572 547 1280 294
Fragments arranged by size:
1387 1280 731 572 547 516 294 160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3150 3185 4576 4611 5427 5462 5487
Size: 3150 35 1391 35 816 35 25
Fragments arranged by size:
3150 1391 816 35 35 35 25
DraIII CAC_nnn'GTG
Cuts at: 0 5042 5417 5487
Size: 5042 375 70
Fragments arranged by size:
5042 375 70
DrdI GACnn_nn'nnGTC
Cuts at: 0 1830 5487
Size: 1830 3657
EaeI y'GGCC_r
Cuts at: 0 1137 2397 3491 3624 3681 3818 4107 4122
Size: 1137 1260 1094 133 57 137 289 15
Cuts at: 4122 4185 4211 4393 4709 4799 5366 5487
Size: 63 26 182 316 90 567 121
Fragments arranged by size:
1260 1137 1094 567 316 289 182 137
133 121 90 63 57 26 15
EarI CTCTTCn'nnn_
Cuts at: 0 428 1352 1673 2418 2747 3500 5487
Size: 428 924 321 745 329 753 1987
Fragments arranged by size:
1987 924 753 745 428 329 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2022 3790 3930 5299 5487
Size: 2022 1768 140 1369 188
Fragments arranged by size:
2022 1768 1369 188 140
EcoICRI GAG'CTC
Cuts at: 0 66 1068 5487
Size: 66 1002 4419
Fragments arranged by size:
4419 1002 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 862 1108 1123 1487 1914 2117 2134
Size: 334 528 246 15 364 427 203 17
Cuts at: 2134 2177 2222 2938 3068 3248 3439 3502 3577
Size: 43 45 716 130 180 191 63 75
Cuts at: 3577 3591 3605 4042 5260 5487
Size: 14 14 437 1218 227
Fragments arranged by size:
1218 716 528 437 427 364 334 246
227 203 191 180 130 75 63 45
43 17 15 14 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4910 5487
Size: 4910 577
EcoO109I rG'GnC_Cy
Cuts at: 0 317 535 562 1362 1844 1940 2519 4929
Size: 317 218 27 800 482 96 579 2410
Cuts at: 4929 5171 5275 5393 5487
Size: 242 104 118 94
Fragments arranged by size:
2410 800 579 482 317 242 218 118
104 96 94 27
EcoRI G'AATT_C
Cuts at: 0 3036 5487
Size: 3036 2451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2001 4713 5487
Size: 2001 2712 774
Fragments arranged by size:
2712 2001 774
FspI TGC'GCA
Cuts at: 0 1522 5487
Size: 1522 3965
HaeII r_GCGC'y
Cuts at: 0 45 2703 2851 5052 5193 5487
Size: 45 2658 148 2201 141 294
Fragments arranged by size:
2658 2201 294 148 141 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 349 381 436 468 3052 3084
Size: 276 32 41 32 55 32 2584 32
Cuts at: 3084 4348 4380 4549 4581 4918 4950 5487
Size: 1264 32 169 32 337 32 537
Fragments arranged by size:
2584 1264 537 337 276 169 55 41
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 192 5487
Size: 192 5295
HpaI GTT'AAC
Cuts at: 0 192 5487
Size: 192 5295
KasI G'GCGC_C
Cuts at: 0 2699 5487
Size: 2699 2788
MfeI C'AATT_G
Cuts at: 0 4255 5487
Size: 4255 1232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 181 3113 4031 5487
Size: 56 125 2932 918 1456
Fragments arranged by size:
2932 1456 918 125 56
MscI TGG'CCA
Cuts at: 0 2399 3493 3820 4187 4711 4801 5487
Size: 2399 1094 327 367 524 90 686
Fragments arranged by size:
2399 1094 686 524 367 327 90
MslI CAynn'nnrTG
Cuts at: 0 950 1010 2368 2876 3001 4087 4499 5487
Size: 950 60 1358 508 125 1086 412 988
Fragments arranged by size:
1358 1086 988 950 508 412 125 60
MspA1I CmG'CkG
Cuts at: 0 26 1124 1227 2045 2074 2829 2835 3158
Size: 26 1098 103 818 29 755 6 323
Cuts at: 3158 3257 3611 3776 3816 3831 3899 3980 4087
Size: 99 354 165 40 15 68 81 107
Cuts at: 4087 4210 4572 5033 5230 5487
Size: 123 362 461 197 257
Fragments arranged by size:
1098 818 755 461 362 354 323 257
197 165 123 107 103 99 81 68
40 29 26 15 6
NaeI GCC'GGC
Cuts at: 0 1993 3645 3933 5370 5487
Size: 1993 1652 288 1437 117
Fragments arranged by size:
1993 1652 1437 288 117
NarI GG'CG_CC
Cuts at: 0 2700 5487
Size: 2700 2787
NcoI C'CATG_G
Cuts at: 0 260 1964 2996 3186 5000 5487
Size: 260 1704 1032 190 1814 487
Fragments arranged by size:
1814 1704 1032 487 260 190
NdeI CA'TA_TG
Cuts at: 0 278 5487
Size: 278 5209
NgoMIV G'CCGG_C
Cuts at: 0 1991 3643 3931 5368 5487
Size: 1991 1652 288 1437 119
Fragments arranged by size:
1991 1652 1437 288 119
NspI r_CATG'y
Cuts at: 0 292 865 2468 5487
Size: 292 573 1603 3019
Fragments arranged by size:
3019 1603 573 292
PasI CC'CwG_GG
Cuts at: 0 467 2671 5487
Size: 467 2204 2816
Fragments arranged by size:
2816 2204 467
PflMI CCAn_nnn'nTGG
Cuts at: 0 786 1965 2395 3414 4183 4797 5487
Size: 786 1179 430 1019 769 614 690
Fragments arranged by size:
1179 1019 786 769 690 614 430
PfoI T'CCnGG_A
Cuts at: 0 3455 5487
Size: 3455 2032
PmlI CAC'GTG
Cuts at: 0 1530 5487
Size: 1530 3957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4418 4450 5487
Size: 4418 32 1037
Fragments arranged by size:
4418 1037 32
PpuMI rG'GwC_Cy
Cuts at: 0 535 562 1362 5171 5393 5487
Size: 535 27 800 3809 222 94
Fragments arranged by size:
3809 800 535 222 94 27
PshAI GACnn'nnGTC
Cuts at: 0 1195 5487
Size: 1195 4292
PspOMI G'GGCC_C
Cuts at: 0 3334 3376 5487
Size: 3334 42 2111
Fragments arranged by size:
3334 2111 42
PstI C_TGCA'G
Cuts at: 0 2801 2834 2958 3371 3781 4577 4814 5203
Size: 2801 33 124 413 410 796 237 389
Cuts at: 5203 5487
Size: 284
Fragments arranged by size:
2801 796 413 410 389 284 237 124
33
PvuII CAG'CTG
Cuts at: 0 26 1227 2829 3611 3816 3980 4087 4572
Size: 26 1201 1602 782 205 164 107 485
Cuts at: 4572 5230 5487
Size: 658 257
Fragments arranged by size:
1602 1201 782 658 485 257 205 164
107 26
SacI G_AGCT'C
Cuts at: 0 68 1070 5487
Size: 68 1002 4417
Fragments arranged by size:
4417 1002 68
SanDI GG'GwC_CC
Cuts at: 0 562 5393 5487
Size: 562 4831 94
Fragments arranged by size:
4831 562 94
SbfI CC_TGCA'GG
Cuts at: 0 4814 5487
Size: 4814 673
SexAI A'CCwGG_T
Cuts at: 0 4892 5487
Size: 4892 595
SfcI C'TryA_G
Cuts at: 0 1728 2685 2797 2830 2954 3367 3777 4573
Size: 1728 957 112 33 124 413 410 796
Cuts at: 4573 4810 5199 5487
Size: 237 389 288
Fragments arranged by size:
1728 957 796 413 410 389 288 237
124 112 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4193 5487
Size: 4193 1294
SfoI GGC'GCC
Cuts at: 0 2701 5487
Size: 2701 2786
SgrAI Cr'CCGG_yG
Cuts at: 0 2696 3743 3950 5487
Size: 2696 1047 207 1537
Fragments arranged by size:
2696 1537 1047 207
SmaI CCC'GGG
Cuts at: 0 180 919 2089 4825 5269 5479 5487
Size: 180 739 1170 2736 444 210 8
Fragments arranged by size:
2736 1170 739 444 210 180 8
SmlI C'TyrA_G
Cuts at: 0 268 1597 2221 3071 3733 5155 5487
Size: 268 1329 624 850 662 1422 332
Fragments arranged by size:
1422 1329 850 662 624 332 268
SphI G_CATG'C
Cuts at: 0 865 5487
Size: 865 4622
StuI AGG'CCT
Cuts at: 0 2111 2613 3700 3796 4196 5218 5487
Size: 2111 502 1087 96 400 1022 269
Fragments arranged by size:
2111 1087 1022 502 400 269 96
StyI C'CwwG_G
Cuts at: 0 260 329 1840 1964 2975 2996 3092 3186
Size: 260 69 1511 124 1011 21 96 94
Cuts at: 3186 3707 4300 4688 5000 5487
Size: 521 593 388 312 487
Fragments arranged by size:
1511 1011 593 521 487 388 312 260
124 96 94 69 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 545 697 1360 2543 4249 4379 4679 5053
Size: 545 152 663 1183 1706 130 300 374
Cuts at: 5053 5487
Size: 434
Fragments arranged by size:
1706 1183 663 545 434 374 300 152
130
TatI w'GTAC_w
Cuts at: 0 3974 5487
Size: 3974 1513
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 925 957 1648 1680 2624 2656 4371 4403
Size: 925 32 691 32 944 32 1715 32
Cuts at: 4403 4407 4439 4949 4976 4981 5008 5487
Size: 4 32 510 27 5 27 479
Fragments arranged by size:
1715 944 925 691 510 479 32 32
32 32 32 27 27 5 4
Tth111I GACn'n_nGTC
Cuts at: 0 363 3016 4474 5487
Size: 363 2653 1458 1013
Fragments arranged by size:
2653 1458 1013 363
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1374 2716 4288 5487
Size: 1374 1342 1572 1199
Fragments arranged by size:
1572 1374 1342 1199
XmaI C'CCGG_G
Cuts at: 0 178 917 2087 4823 5267 5477 5487
Size: 178 739 1170 2736 444 210 10
Fragments arranged by size:
2736 1170 739 444 210 178 10
XmnI GAAnn'nnTTC
Cuts at: 0 3796 5487
Size: 3796 1691
ZraI GAC'GTC
Cuts at: 0 4997 5487
Size: 4997 490
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflIII AgeI AhdI AscI AseI AsiSI
BamHI BciVI BclI BfrBI BmgBI BmtI BsaBI BsiEI
BsiWI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I
ClaI DraI EagI EcoRV FseI FspAI HindIII KpnI
MluI NheI NotI NruI NsiI PacI PciI PmeI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
ScaI SgfI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI