Restriction map for
pF1KSDA0286
(Linear) (Six-base) MAPSORT of: KIAA0286.seq Check: 1174 from: 1 to: 1332
KIAA0286.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 982 1332
Size: 982 350
AccI GT'mk_AC
Cuts at: 0 116 1332
Size: 116 1216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 331 1332
Size: 139 192 1001
Fragments arranged by size:
1001 192 139
AleI CACnn'nnGTG
Cuts at: 0 208 289 1332
Size: 208 81 1043
Fragments arranged by size:
1043 208 81
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 331 363 1332
Size: 331 32 969
Fragments arranged by size:
969 331 32
AlwNI CAG_nnn'CTG
Cuts at: 0 963 1332
Size: 963 369
ApaI G_GGCC'C
Cuts at: 0 50 809 1332
Size: 50 759 523
Fragments arranged by size:
759 523 50
ApoI r'AATT_y
Cuts at: 0 551 711 1090 1332
Size: 551 160 379 242
Fragments arranged by size:
551 379 242 160
AvaI C'yCGr_G
Cuts at: 0 43 57 1332
Size: 43 14 1275
Fragments arranged by size:
1275 43 14
BanII G_rGCy'C
Cuts at: 0 50 58 809 1085 1332
Size: 50 8 751 276 247
Fragments arranged by size:
751 276 247 50 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1129 1332
Size: 1129 203
BciVI GTATCCnnnnn_n'
Cuts at: 0 239 1037 1332
Size: 239 798 295
Fragments arranged by size:
798 295 239
BglII A'GATC_T
Cuts at: 0 722 1332
Size: 722 610
BlpI GC'TnA_GC
Cuts at: 0 538 1332
Size: 538 794
Bme1580I G_kGCm'C
Cuts at: 0 50 809 1332
Size: 50 759 523
Fragments arranged by size:
759 523 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 578 1332
Size: 578 754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1302 1332
Size: 1302 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 1332
Size: 747 585
BsaI GGTCTCn'nnnn_
Cuts at: 0 30 1332
Size: 30 1302
BsaBI GATnn'nnATC
Cuts at: 0 250 1332
Size: 250 1082
BsaWI w'CCGG_w
Cuts at: 0 467 1332
Size: 467 865
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 1093 1291 1294 1332
Size: 316 777 198 3 38
Fragments arranged by size:
777 316 198 38 3
BsiHKAI G_wGCw'C
Cuts at: 0 1085 1332
Size: 1085 247
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1024 1332
Size: 1024 308
Bsp1286I G_dGCh'C
Cuts at: 0 50 58 809 1085 1332
Size: 50 8 751 276 247
Fragments arranged by size:
751 276 247 50 8
BspEI T'CCGG_A
Cuts at: 0 467 1332
Size: 467 865
BspHI T'CATG_A
Cuts at: 0 776 1332
Size: 776 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 421 842 982 1332
Size: 421 421 140 350
Fragments arranged by size:
421 421 350 140
BsrDI GCAATG_nn'
Cuts at: 0 920 1332
Size: 920 412
BsrFI r'CCGG_y
Cuts at: 0 30 1332
Size: 30 1302
BsrGI T'GTAC_A
Cuts at: 0 965 1332
Size: 965 367
BstYI r'GATC_y
Cuts at: 0 251 722 1332
Size: 251 471 610
Fragments arranged by size:
610 471 251
DraI TTT'AAA
Cuts at: 0 359 708 1332
Size: 359 349 624
Fragments arranged by size:
624 359 349
EarI CTCTTCn'nnn_
Cuts at: 0 1182 1332
Size: 1182 150
EcoICRI GAG'CTC
Cuts at: 0 1083 1332
Size: 1083 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 331 747 1332
Size: 139 192 416 585
Fragments arranged by size:
585 416 192 139
EcoO109I rG'GnC_Cy
Cuts at: 0 47 806 1332
Size: 47 759 526
Fragments arranged by size:
759 526 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 494 531 563 1116 1148 1252 1284
Size: 462 32 37 32 553 32 104 32
Cuts at: 1284 1332
Size: 48
Fragments arranged by size:
553 462 104 48 37 32 32 32
32
HincII GTy'rAC
Cuts at: 0 387 1332
Size: 387 945
HpaI GTT'AAC
Cuts at: 0 387 1332
Size: 387 945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1332
Size: 750 582
MslI CAynn'nnrTG
Cuts at: 0 208 289 661 1332
Size: 208 81 372 671
Fragments arranged by size:
671 372 208 81
MspA1I CmG'CkG
Cuts at: 0 127 855 1332
Size: 127 728 477
Fragments arranged by size:
728 477 127
NaeI GCC'GGC
Cuts at: 0 32 1332
Size: 32 1300
NgoMIV G'CCGG_C
Cuts at: 0 30 1332
Size: 30 1302
PasI CC'CwG_GG
Cuts at: 0 50 1332
Size: 50 1282
PspOMI G'GGCC_C
Cuts at: 0 46 805 1332
Size: 46 759 527
Fragments arranged by size:
759 527 46
PstI C_TGCA'G
Cuts at: 0 979 1332
Size: 979 353
PvuII CAG'CTG
Cuts at: 0 127 855 1332
Size: 127 728 477
Fragments arranged by size:
728 477 127
SacI G_AGCT'C
Cuts at: 0 1085 1332
Size: 1085 247
SapI GCTCTTCn'nnn_
Cuts at: 0 1182 1332
Size: 1182 150
SfcI C'TryA_G
Cuts at: 0 975 1332
Size: 975 357
SmaI CCC'GGG
Cuts at: 0 45 1332
Size: 45 1287
SpeI A'CTAG_T
Cuts at: 0 699 1332
Size: 699 633
StyI C'CwwG_G
Cuts at: 0 809 1332
Size: 809 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 1332
Size: 301 1031
TatI w'GTAC_w
Cuts at: 0 449 931 965 1332
Size: 449 482 34 367
Fragments arranged by size:
482 449 367 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1332
Size: 2 32 1298
Fragments arranged by size:
1298 32 2
XmaI C'CCGG_G
Cuts at: 0 43 1332
Size: 43 1289
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI ApoI
AvaI BanII BbsI BciVI BglII BlpI Bme1580I BmrI
BplI BpmI BsaI BsaBI BsaWI BseRI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstYI
DraI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII HpaI
MmeI MslI MspA1I NaeI NgoMIV PasI PspOMI PstI
PvuII SacI SapI SfcI SmaI SpeI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BclI BfrBI BglI BmgBI
BmtI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI