Restriction map for
pF1KSDA0282
(Linear) (Six-base) MAPSORT of: KIAA0282.seq Check: 587 from: 1 to: 1992
KIAA0282.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 388 691 1992
Size: 388 303 1301
Fragments arranged by size:
1301 388 303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1992
Size: 1057 935
AfeI AGC'GCT
Cuts at: 0 1371 1992
Size: 1371 621
AhdI GACnn_n'nnGTC
Cuts at: 0 1091 1992
Size: 1091 901
AlwNI CAG_nnn'CTG
Cuts at: 0 130 840 1002 1992
Size: 130 710 162 990
Fragments arranged by size:
990 710 162 130
ApaI G_GGCC'C
Cuts at: 0 608 1992
Size: 608 1384
ApaLI G'TGCA_C
Cuts at: 0 1177 1491 1992
Size: 1177 314 501
Fragments arranged by size:
1177 501 314
AvaI C'yCGr_G
Cuts at: 0 599 1532 1992
Size: 599 933 460
Fragments arranged by size:
933 599 460
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 357 390 1992
Size: 357 33 1602
Fragments arranged by size:
1602 357 33
BanI G'GyrC_C
Cuts at: 0 357 519 627 1407 1992
Size: 357 162 108 780 585
Fragments arranged by size:
780 585 357 162 108
BanII G_rGCy'C
Cuts at: 0 59 163 425 608 667 1748 1992
Size: 59 104 262 183 59 1081 244
Fragments arranged by size:
1081 262 244 183 104 59 59
BbeI G_GCGC'C
Cuts at: 0 523 1992
Size: 523 1469
BbsI GAAGACnn'nnnn_
Cuts at: 0 552 1498 1717 1992
Size: 552 946 219 275
Fragments arranged by size:
946 552 275 219
BciVI GTATCCnnnnn_n'
Cuts at: 0 1414 1992
Size: 1414 578
BglI GCCn_nnn'nGGC
Cuts at: 0 603 1992
Size: 603 1389
BglII A'GATC_T
Cuts at: 0 1055 1992
Size: 1055 937
BlpI GC'TnA_GC
Cuts at: 0 661 1992
Size: 661 1331
Bme1580I G_kGCm'C
Cuts at: 0 362 608 632 1181 1412 1495 1992
Size: 362 246 24 549 231 83 497
Fragments arranged by size:
549 497 362 246 231 83 24
BmgBI CAC'GTC
Cuts at: 0 1806 1992
Size: 1806 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1749 1992
Size: 381 1368 243
Fragments arranged by size:
1368 381 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 759 1298 1363 1450 1992
Size: 721 38 539 65 87 542
Fragments arranged by size:
721 542 539 87 65 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1263 1992
Size: 481 782 729
Fragments arranged by size:
782 729 481
BsaI GGTCTCn'nnnn_
Cuts at: 0 174 681 1099 1992
Size: 174 507 418 893
Fragments arranged by size:
893 507 418 174
BsaHI Gr'CG_yC
Cuts at: 0 293 520 1160 1992
Size: 293 227 640 832
Fragments arranged by size:
832 640 293 227
BsaWI w'CCGG_w
Cuts at: 0 1868 1992
Size: 1868 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1200 1230 1474 1504 1992
Size: 1200 30 244 30 488
Fragments arranged by size:
1200 488 244 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 31 775 1939 1992
Size: 19 12 744 1164 53
Fragments arranged by size:
1164 744 53 19 12
BseYI C'CCAG_C
Cuts at: 0 995 1320 1992
Size: 995 325 672
Fragments arranged by size:
995 672 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 669 1050 1992
Size: 136 533 381 942
Fragments arranged by size:
942 533 381 136
BsiHKAI G_wGCw'C
Cuts at: 0 1031 1181 1495 1748 1992
Size: 1031 150 314 253 244
Fragments arranged by size:
1031 314 253 244 150
Bsp1286I G_dGCh'C
Cuts at: 0 59 163 362 425 608 632 667 1031
Size: 59 104 199 63 183 24 35 364
Cuts at: 1031 1181 1412 1495 1748 1992
Size: 150 231 83 253 244
Fragments arranged by size:
364 253 244 231 199 183 150 104
83 63 59 35 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 388 691 1992
Size: 388 303 1301
Fragments arranged by size:
1301 388 303
BsrDI GCAATG_nn'
Cuts at: 0 733 1856 1992
Size: 733 1123 136
Fragments arranged by size:
1123 733 136
BsrFI r'CCGG_y
Cuts at: 0 324 342 1992
Size: 324 18 1650
Fragments arranged by size:
1650 324 18
BssSI C'ACGA_G
Cuts at: 0 694 1992
Size: 694 1298
BstAPI GCAn_nnn'nTGC
Cuts at: 0 719 1992
Size: 719 1273
BstYI r'GATC_y
Cuts at: 0 911 1055 1992
Size: 911 144 937
Fragments arranged by size:
937 911 144
BtgI C'CryG_G
Cuts at: 0 155 426 488 1229 1403 1992
Size: 155 271 62 741 174 589
Fragments arranged by size:
741 589 271 174 155 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 569 1992
Size: 569 1423
BtsI GCAGTG_nn'
Cuts at: 0 1062 1992
Size: 1062 930
ClaI AT'CG_AT
Cuts at: 0 971 1992
Size: 971 1021
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1218 1253 1838 1873 1992
Size: 1218 35 585 35 119
Fragments arranged by size:
1218 585 119 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 126 1992
Size: 126 1866
EaeI y'GGCC_r
Cuts at: 0 326 1992
Size: 326 1666
EarI CTCTTCn'nnn_
Cuts at: 0 8 1167 1992
Size: 8 1159 825
Fragments arranged by size:
1159 825 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 235 1992
Size: 235 1757
EcoICRI GAG'CTC
Cuts at: 0 1746 1992
Size: 1746 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 759 1057 1298 1363 1450 1992
Size: 721 38 298 241 65 87 542
Fragments arranged by size:
721 542 298 241 87 65 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 1992
Size: 1186 806
EcoO109I rG'GnC_Cy
Cuts at: 0 604 605 1944 1992
Size: 604 1 1339 48
Fragments arranged by size:
1339 604 48 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1734 1992
Size: 1734 258
FspI TGC'GCA
Cuts at: 0 895 1992
Size: 895 1097
HaeII r_GCGC'y
Cuts at: 0 260 523 841 1373 1992
Size: 260 263 318 532 619
Fragments arranged by size:
619 532 318 263 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 1040 1042 1072 1074 1077 1109
Size: 118 32 890 2 30 2 3 32
Cuts at: 1109 1176 1208 1612 1644 1992
Size: 67 32 404 32 348
Fragments arranged by size:
890 404 348 118 67 32 32 32
32 30 3 2 2
HincII GTy'rAC
Cuts at: 0 1014 1992
Size: 1014 978
HindIII A'AGCT_T
Cuts at: 0 1304 1841 1992
Size: 1304 537 151
Fragments arranged by size:
1304 537 151
KasI G'GCGC_C
Cuts at: 0 519 1992
Size: 519 1473
MfeI C'AATT_G
Cuts at: 0 1937 1992
Size: 1937 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1992
Size: 197 1795
MslI CAynn'nnrTG
Cuts at: 0 112 538 1804 1992
Size: 112 426 1266 188
Fragments arranged by size:
1266 426 188 112
MspA1I CmG'CkG
Cuts at: 0 157 227 428 490 891 999 1284 1341
Size: 157 70 201 62 401 108 285 57
Cuts at: 1341 1992
Size: 651
Fragments arranged by size:
651 401 285 201 157 108 70 62
57
NarI GG'CG_CC
Cuts at: 0 520 1992
Size: 520 1472
NspI r_CATG'y
Cuts at: 0 717 1992
Size: 717 1275
PasI CC'CwG_GG
Cuts at: 0 639 1992
Size: 639 1353
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 756 1002 1284 1992
Size: 699 57 246 282 708
Fragments arranged by size:
708 699 282 246 57
PfoI T'CCnGG_A
Cuts at: 0 407 914 1381 1460 1992
Size: 407 507 467 79 532
Fragments arranged by size:
532 507 467 407 79
PpuMI rG'GwC_Cy
Cuts at: 0 1944 1992
Size: 1944 48
PshAI GACnn'nnGTC
Cuts at: 0 1244 1582 1992
Size: 1244 338 410
Fragments arranged by size:
1244 410 338
PspOMI G'GGCC_C
Cuts at: 0 604 1992
Size: 604 1388
PvuII CAG'CTG
Cuts at: 0 891 999 1284 1341 1992
Size: 891 108 285 57 651
Fragments arranged by size:
891 651 285 108 57
SacI G_AGCT'C
Cuts at: 0 1748 1992
Size: 1748 244
SacII CC_GC'GG
Cuts at: 0 158 429 491 1992
Size: 158 271 62 1501
Fragments arranged by size:
1501 271 158 62
ScaI AGT'ACT
Cuts at: 0 1105 1992
Size: 1105 887
SfcI C'TryA_G
Cuts at: 0 1336 1677 1992
Size: 1336 341 315
Fragments arranged by size:
1336 341 315
SfoI GGC'GCC
Cuts at: 0 521 1992
Size: 521 1471
SmaI CCC'GGG
Cuts at: 0 601 1992
Size: 601 1391
SmlI C'TyrA_G
Cuts at: 0 496 1242 1992
Size: 496 746 750
Fragments arranged by size:
750 746 496
StuI AGG'CCT
Cuts at: 0 1543 1992
Size: 1543 449
StyI C'CwwG_G
Cuts at: 0 524 872 1219 1724 1992
Size: 524 348 347 505 268
Fragments arranged by size:
524 505 348 347 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 1992
Size: 346 1646
TatI w'GTAC_w
Cuts at: 0 716 1103 1992
Size: 716 387 889
Fragments arranged by size:
889 716 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1200 1232 1474 1506 1992
Size: 1200 32 242 32 486
Fragments arranged by size:
1200 486 242 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 879 1095 1281 1992
Size: 621 258 216 186 711
Fragments arranged by size:
711 621 258 216 186
XmaI C'CCGG_G
Cuts at: 0 599 1992
Size: 599 1393
Enzymes that do cut:
AarI AcuI AfeI AhdI AlwNI ApaI ApaLI AvaI
BaeI BanI BanII BbeI BbsI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BssSI BstAPI BstYI BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MslI MspA1I NarI NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PvuII SacI SacII
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BamHI
BbvCI BcgI BclI BfrBI BmtI BplI Bpu10I BsaAI
BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I DraI
DraIII EagI EcoRI EcoRV FseI FspAI HpaI KpnI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PstI PvuI RsrII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI