Restriction map for
pF1KSDA0278
(Linear) (Six-base) MAPSORT of: KIAA0278.seq Check: 2683 from: 1 to: 1188
KIAA0278.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 51 152 156 678 1188
Size: 51 101 4 522 510
Fragments arranged by size:
522 510 101 51 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1188
Size: 202 986
AfeI AGC'GCT
Cuts at: 0 256 319 373 1188
Size: 256 63 54 815
Fragments arranged by size:
815 256 63 54
AflIII A'CryG_T
Cuts at: 0 341 1188
Size: 341 847
AgeI A'CCGG_T
Cuts at: 0 194 1188
Size: 194 994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1078 1188
Size: 1078 110
AlwNI CAG_nnn'CTG
Cuts at: 0 538 903 1188
Size: 538 365 285
Fragments arranged by size:
538 365 285
AvaI C'yCGr_G
Cuts at: 0 43 463 550 650 839 1183 1188
Size: 43 420 87 100 189 344 5
Fragments arranged by size:
420 344 189 100 87 43 5
BanI G'GyrC_C
Cuts at: 0 140 979 1188
Size: 140 839 209
Fragments arranged by size:
839 209 140
BanII G_rGCy'C
Cuts at: 0 32 563 778 1188
Size: 32 531 215 410
Fragments arranged by size:
531 410 215 32
BglI GCCn_nnn'nGGC
Cuts at: 0 1093 1188
Size: 1093 95
BglII A'GATC_T
Cuts at: 0 635 1188
Size: 635 553
Bme1580I G_kGCm'C
Cuts at: 0 239 832 982 1188
Size: 239 593 150 206
Fragments arranged by size:
593 239 206 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 798 1188
Size: 798 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 232 334 388 409 1054 1096 1188
Size: 136 96 102 54 21 645 42 92
Fragments arranged by size:
645 136 102 96 92 54 42 21
Bpu10I CC'TnA_GC
Cuts at: 0 661 1188
Size: 661 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1188
Size: 982 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 1123 1188
Size: 292 831 65
Fragments arranged by size:
831 292 65
BsaAI yAC'GTr
Cuts at: 0 123 234 342 939 972 1188
Size: 123 111 108 597 33 216
Fragments arranged by size:
597 216 123 111 108 33
BsaWI w'CCGG_w
Cuts at: 0 14 194 1172 1188
Size: 14 180 978 16
Fragments arranged by size:
978 180 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 688 718 967 970 1188
Size: 688 30 249 3 218
Fragments arranged by size:
688 249 218 30 3
BseYI C'CCAG_C
Cuts at: 0 320 374 531 572 613 1178 1188
Size: 320 54 157 41 41 565 10
Fragments arranged by size:
565 320 157 54 41 41 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1084 1188
Size: 175 909 104
Fragments arranged by size:
909 175 104
BsiEI CG_ry'CG
Cuts at: 0 199 203 384 1088 1097 1188
Size: 199 4 181 704 9 91
Fragments arranged by size:
704 199 181 91 9 4
BsiHKAI G_wGCw'C
Cuts at: 0 122 1188
Size: 122 1066
Bsp1286I G_dGCh'C
Cuts at: 0 32 122 239 563 778 832 982 1188
Size: 32 90 117 324 215 54 150 206
Fragments arranged by size:
324 215 206 150 117 90 54 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 152 156 678 875 1188
Size: 51 101 4 522 197 313
Fragments arranged by size:
522 313 197 101 51 4
BsrFI r'CCGG_y
Cuts at: 0 137 194 744 1092 1096 1188
Size: 137 57 550 348 4 92
Fragments arranged by size:
550 348 137 92 57 4
BssSI C'ACGA_G
Cuts at: 0 240 478 1188
Size: 240 238 710
Fragments arranged by size:
710 240 238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 1032 1188
Size: 168 864 156
Fragments arranged by size:
864 168 156
BstYI r'GATC_y
Cuts at: 0 635 722 1188
Size: 635 87 466
Fragments arranged by size:
635 466 87
BtgI C'CryG_G
Cuts at: 0 49 395 446 586 1160 1188
Size: 49 346 51 140 574 28
Fragments arranged by size:
574 346 140 51 49 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 288 1188
Size: 288 900
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 1188
Size: 502 686
EaeI y'GGCC_r
Cuts at: 0 65 149 746 1085 1094 1163 1188
Size: 65 84 597 339 9 69 25
Fragments arranged by size:
597 339 84 69 65 25 9
EagI C'GGCC_G
Cuts at: 0 1085 1094 1188
Size: 1085 9 94
Fragments arranged by size:
1085 94 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 144 1141 1188
Size: 144 997 47
Fragments arranged by size:
997 144 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 202 232 334 388 409 1054 1096
Size: 136 66 30 102 54 21 645 42
Cuts at: 1096 1188
Size: 92
Fragments arranged by size:
645 136 102 92 66 54 42 30
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1078 1188
Size: 1078 110
EcoO109I rG'GnC_Cy
Cuts at: 0 460 591 645 918 1188
Size: 460 131 54 273 270
Fragments arranged by size:
460 273 270 131 54
FseI GG_CCGG'CC
Cuts at: 0 748 1100 1188
Size: 748 352 88
Fragments arranged by size:
748 352 88
HaeII r_GCGC'y
Cuts at: 0 258 321 375 1188
Size: 258 63 54 813
Fragments arranged by size:
813 258 63 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 714 746 833 865 1188
Size: 714 32 87 32 323
Fragments arranged by size:
714 323 87 32 32
MscI TGG'CCA
Cuts at: 0 67 1165 1188
Size: 67 1098 23
Fragments arranged by size:
1098 67 23
MslI CAynn'nnrTG
Cuts at: 0 1060 1188
Size: 1060 128
MspA1I CmG'CkG
Cuts at: 0 23 51 290 538 855 891 1188
Size: 23 28 239 248 317 36 297
Fragments arranged by size:
317 297 248 239 36 28 23
NaeI GCC'GGC
Cuts at: 0 746 1094 1098 1188
Size: 746 348 4 90
Fragments arranged by size:
746 348 90 4
NgoMIV G'CCGG_C
Cuts at: 0 744 1092 1096 1188
Size: 744 348 4 92
Fragments arranged by size:
744 348 92 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 538 755 972 1188
Size: 538 217 217 216
Fragments arranged by size:
538 217 217 216
PmlI CAC'GTG
Cuts at: 0 123 342 1188
Size: 123 219 846
Fragments arranged by size:
846 219 123
PpuMI rG'GwC_Cy
Cuts at: 0 460 591 645 918 1188
Size: 460 131 54 273 270
Fragments arranged by size:
460 273 270 131 54
PstI C_TGCA'G
Cuts at: 0 89 818 989 1188
Size: 89 729 171 199
Fragments arranged by size:
729 199 171 89
SacII CC_GC'GG
Cuts at: 0 52 1188
Size: 52 1136
SanDI GG'GwC_CC
Cuts at: 0 460 591 1188
Size: 460 131 597
Fragments arranged by size:
597 460 131
ScaI AGT'ACT
Cuts at: 0 679 709 1188
Size: 679 30 479
Fragments arranged by size:
679 479 30
SfcI C'TryA_G
Cuts at: 0 85 814 985 1188
Size: 85 729 171 203
Fragments arranged by size:
729 203 171 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1093 1188
Size: 1093 95
SmaI CCC'GGG
Cuts at: 0 45 552 1188
Size: 45 507 636
Fragments arranged by size:
636 507 45
SmlI C'TyrA_G
Cuts at: 0 650 997 1188
Size: 650 347 191
Fragments arranged by size:
650 347 191
SrfI GCCC'GGGC
Cuts at: 0 552 1188
Size: 552 636
StuI AGG'CCT
Cuts at: 0 277 1188
Size: 277 911
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 780 1188
Size: 189 591 408
Fragments arranged by size:
591 408 189
TatI w'GTAC_w
Cuts at: 0 677 707 818 1188
Size: 677 30 111 370
Fragments arranged by size:
677 370 111 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 1188
Size: 427 32 729
Fragments arranged by size:
729 427 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 309 621 1188
Size: 309 312 567
Fragments arranged by size:
567 312 309
XhoI C'TCGA_G
Cuts at: 0 650 1188
Size: 650 538
XmaI C'CCGG_G
Cuts at: 0 43 550 1188
Size: 43 507 638
Fragments arranged by size:
638 507 43
XmnI GAAnn'nnTTC
Cuts at: 0 808 1188
Size: 808 380
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AlfI AlwNI AvaI
BanI BanII BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstXI BstYI BtgI
BtgZI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV
PflMI PmlI PpuMI PstI SacII SanDI ScaI SfcI
SfiI SmaI SmlI SrfI StuI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BsaBI BsaHI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII EarI EcoICRI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SalI SapI SbfI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI StyI SwaI Tth111I XbaI ZraI