Restriction map for
pF1KSDA0275
(Linear) (Six-base) MAPSORT of: KIAA0275.seq Check: 9201 from: 1 to: 1272
KIAA0275.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AgeI A'CCGG_T
Cuts at: 0 598 1272
Size: 598 674
AlwNI CAG_nnn'CTG
Cuts at: 0 609 1272
Size: 609 663
ApaI G_GGCC'C
Cuts at: 0 535 1272
Size: 535 737
AvaI C'yCGr_G
Cuts at: 0 9 1002 1146 1272
Size: 9 993 144 126
Fragments arranged by size:
993 144 126 9
BanII G_rGCy'C
Cuts at: 0 86 535 1120 1272
Size: 86 449 585 152
Fragments arranged by size:
585 449 152 86
BciVI GTATCCnnnnn_n'
Cuts at: 0 243 1272
Size: 243 1029
BglI GCCn_nnn'nGGC
Cuts at: 0 700 1272
Size: 700 572
BlpI GC'TnA_GC
Cuts at: 0 678 1272
Size: 678 594
Bme1580I G_kGCm'C
Cuts at: 0 535 1272
Size: 535 737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 502 939 970 1108 1272
Size: 370 132 437 31 138 164
Fragments arranged by size:
437 370 164 138 132 31
Bpu10I CC'TnA_GC
Cuts at: 0 499 1272
Size: 499 773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1272
Size: 70 1202
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 894 1272
Size: 91 803 378
Fragments arranged by size:
803 378 91
BsaHI Gr'CG_yC
Cuts at: 0 74 1272
Size: 74 1198
BsaWI w'CCGG_w
Cuts at: 0 598 1028 1272
Size: 598 430 244
Fragments arranged by size:
598 430 244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 1272
Size: 382 30 860
Fragments arranged by size:
860 382 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1198 1201 1228 1237 1240 1243 1272
Size: 1198 3 27 9 3 3 29
Fragments arranged by size:
1198 29 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 215 425 1080 1172 1272
Size: 215 210 655 92 100
Fragments arranged by size:
655 215 210 100 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 1272
Size: 303 969
BsiEI CG_ry'CG
Cuts at: 0 374 1272
Size: 374 898
BsiHKAI G_wGCw'C
Cuts at: 0 356 1272
Size: 356 916
BsmBI CGTCTCn'nnnn_
Cuts at: 0 179 1189 1272
Size: 179 1010 83
Fragments arranged by size:
1010 179 83
Bsp1286I G_dGCh'C
Cuts at: 0 86 356 535 1120 1272
Size: 86 270 179 585 152
Fragments arranged by size:
585 270 179 152 86
BspHI T'CATG_A
Cuts at: 0 654 1272
Size: 654 618
BsrDI GCAATG_nn'
Cuts at: 0 133 302 1272
Size: 133 169 970
Fragments arranged by size:
970 169 133
BsrFI r'CCGG_y
Cuts at: 0 598 694 1272
Size: 598 96 578
Fragments arranged by size:
598 578 96
BssHII G'CGCG_C
Cuts at: 0 3 1272
Size: 3 1269
BstAPI GCAn_nnn'nTGC
Cuts at: 0 413 1272
Size: 413 859
BstXI CCAn_nnnn'nTGG
Cuts at: 0 711 822 1272
Size: 711 111 450
Fragments arranged by size:
711 450 111
BstYI r'GATC_y
Cuts at: 0 962 1272
Size: 962 310
BtgI C'CryG_G
Cuts at: 0 50 384 548 1272
Size: 50 334 164 724
Fragments arranged by size:
724 334 164 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 537 1142 1272
Size: 537 605 130
Fragments arranged by size:
605 537 130
BtsI GCAGTG_nn'
Cuts at: 0 693 1045 1272
Size: 693 352 227
Fragments arranged by size:
693 352 227
DraIII CAC_nnn'GTG
Cuts at: 0 297 1272
Size: 297 975
EaeI y'GGCC_r
Cuts at: 0 47 62 453 701 809 882 1272
Size: 47 15 391 248 108 73 390
Fragments arranged by size:
391 390 248 108 73 47 15
EarI CTCTTCn'nnn_
Cuts at: 0 793 1272
Size: 793 479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 502 939 970 1108 1272
Size: 370 132 437 31 138 164
Fragments arranged by size:
437 370 164 138 132 31
EcoO109I rG'GnC_Cy
Cuts at: 0 261 531 532 606 1272
Size: 261 270 1 74 666
Fragments arranged by size:
666 270 261 74 1
MscI TGG'CCA
Cuts at: 0 455 1272
Size: 455 817
MspA1I CmG'CkG
Cuts at: 0 36 52 155 321 513 707 734 1055
Size: 36 16 103 166 192 194 27 321
Cuts at: 1055 1080 1272
Size: 25 192
Fragments arranged by size:
321 194 192 192 166 103 36 27
25 16
NaeI GCC'GGC
Cuts at: 0 696 1272
Size: 696 576
NcoI C'CATG_G
Cuts at: 0 384 1272
Size: 384 888
NgoMIV G'CCGG_C
Cuts at: 0 694 1272
Size: 694 578
PasI CC'CwG_GG
Cuts at: 0 309 1272
Size: 309 963
PflMI CCAn_nnn'nTGG
Cuts at: 0 752 1272
Size: 752 520
PfoI T'CCnGG_A
Cuts at: 0 791 965 1272
Size: 791 174 307
Fragments arranged by size:
791 307 174
PpuMI rG'GwC_Cy
Cuts at: 0 261 606 1272
Size: 261 345 666
Fragments arranged by size:
666 345 261
PspOMI G'GGCC_C
Cuts at: 0 531 1272
Size: 531 741
PvuII CAG'CTG
Cuts at: 0 513 734 1080 1272
Size: 513 221 346 192
Fragments arranged by size:
513 346 221 192
SacII CC_GC'GG
Cuts at: 0 53 1272
Size: 53 1219
SmaI CCC'GGG
Cuts at: 0 11 1272
Size: 11 1261
SmlI C'TyrA_G
Cuts at: 0 85 1272
Size: 85 1187
StyI C'CwwG_G
Cuts at: 0 77 257 384 1272
Size: 77 180 127 888
Fragments arranged by size:
888 180 127 77
TatI w'GTAC_w
Cuts at: 0 149 1272
Size: 149 1123
XmaI C'CCGG_G
Cuts at: 0 9 1272
Size: 9 1263
XmnI GAAnn'nnTTC
Cuts at: 0 178 1272
Size: 178 1094
Enzymes that do cut:
AgeI AlwNI ApaI AvaI BanII BciVI BglI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BsrDI BsrFI BssHII BstAPI BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI EarI Eco57MI EcoO109I MscI MspA1I
NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PvuII SacII SmaI SmlI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglII BmgBI BmrI BmtI
BplI BsaAI BsaBI BsiWI BsmI BspEI BspMI BsrBI
BsrGI BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI ZraI