Restriction map for
pF1KSDA0272
(Linear) (Six-base) MAPSORT of: KIAA0272.seq Check: 11 from: 1 to: 2187
KIAA0272.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1126 2187
Size: 1126 1061
AccI GT'mk_AC
Cuts at: 0 563 2187
Size: 563 1624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 577 763 1927 2187
Size: 88 489 186 1164 260
Fragments arranged by size:
1164 489 260 186 88
AflIII A'CryG_T
Cuts at: 0 236 2187
Size: 236 1951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1696 2187
Size: 1696 491
AlwNI CAG_nnn'CTG
Cuts at: 0 783 913 1068 2187
Size: 783 130 155 1119
Fragments arranged by size:
1119 783 155 130
ApaI G_GGCC'C
Cuts at: 0 124 584 903 2187
Size: 124 460 319 1284
Fragments arranged by size:
1284 460 319 124
AvaI C'yCGr_G
Cuts at: 0 439 2187
Size: 439 1748
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 194 227 2187
Size: 194 33 1960
Fragments arranged by size:
1960 194 33
BanI G'GyrC_C
Cuts at: 0 699 2099 2187
Size: 699 1400 88
Fragments arranged by size:
1400 699 88
BanII G_rGCy'C
Cuts at: 0 124 584 665 903 1862 2187
Size: 124 460 81 238 959 325
Fragments arranged by size:
959 460 325 238 124 81
BbeI G_GCGC'C
Cuts at: 0 2103 2187
Size: 2103 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 1130 1840 2187
Size: 1130 710 347
Fragments arranged by size:
1130 710 347
BbvCI CC'TCA_GC
Cuts at: 0 1694 2187
Size: 1694 493
BglI GCCn_nnn'nGGC
Cuts at: 0 2148 2187
Size: 2148 39
BglII A'GATC_T
Cuts at: 0 92 2187
Size: 92 2095
BlpI GC'TnA_GC
Cuts at: 0 289 1536 2187
Size: 289 1247 651
Fragments arranged by size:
1247 651 289
Bme1580I G_kGCm'C
Cuts at: 0 124 584 704 903 1436 2187
Size: 124 460 120 199 533 751
Fragments arranged by size:
751 533 460 199 124 120
BmgBI CAC'GTC
Cuts at: 0 1224 2187
Size: 1224 963
BmrI ACTGGGnnnn_n'
Cuts at: 0 1786 2187
Size: 1786 401
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1896 2187
Size: 1896 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 43 998 1542 2187
Size: 37 6 955 544 645
Fragments arranged by size:
955 645 544 37 6
Bpu10I CC'TnA_GC
Cuts at: 0 1694 2048 2187
Size: 1694 354 139
Fragments arranged by size:
1694 354 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1884 1933 2187
Size: 812 1072 49 254
Fragments arranged by size:
1072 812 254 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 189 2187
Size: 189 1998
BsaHI Gr'CG_yC
Cuts at: 0 2100 2187
Size: 2100 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 841 871 2187
Size: 437 30 374 30 1316
Fragments arranged by size:
1316 437 374 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 613 862 955 1228 1969 2187
Size: 103 510 249 93 273 741 218
Fragments arranged by size:
741 510 273 249 218 103 93
BseYI C'CCAG_C
Cuts at: 0 1172 1411 1462 2187
Size: 1172 239 51 725
Fragments arranged by size:
1172 725 239 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 956 1246 1669 2010 2187
Size: 500 456 290 423 341 177
Fragments arranged by size:
500 456 423 341 290 177
BsiEI CG_ry'CG
Cuts at: 0 708 2121 2187
Size: 708 1413 66
Fragments arranged by size:
1413 708 66
BsiHKAI G_wGCw'C
Cuts at: 0 299 2187
Size: 299 1888
Bsp1286I G_dGCh'C
Cuts at: 0 124 299 584 665 704 903 1436 1862
Size: 124 175 285 81 39 199 533 426
Cuts at: 1862 2187
Size: 325
Fragments arranged by size:
533 426 325 285 199 175 124 81
39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1126 2187
Size: 1126 1061
BsrBI CCG'CTC
Cuts at: 0 1289 1535 2187
Size: 1289 246 652
Fragments arranged by size:
1289 652 246
BsrDI GCAATG_nn'
Cuts at: 0 402 1473 2187
Size: 402 1071 714
Fragments arranged by size:
1071 714 402
BsrFI r'CCGG_y
Cuts at: 0 167 533 1071 2120 2147 2187
Size: 167 366 538 1049 27 40
Fragments arranged by size:
1049 538 366 167 40 27
BssSI C'ACGA_G
Cuts at: 0 52 2187
Size: 52 2135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 280 2187
Size: 280 1907
BstXI CCAn_nnnn'nTGG
Cuts at: 0 931 1002 2073 2187
Size: 931 71 1071 114
Fragments arranged by size:
1071 931 114 71
BstYI r'GATC_y
Cuts at: 0 92 2187
Size: 92 2095
Bsu36I CC'TnA_GG
Cuts at: 0 845 1246 2187
Size: 845 401 941
Fragments arranged by size:
941 845 401
BtgI C'CryG_G
Cuts at: 0 491 576 1817 2187
Size: 491 85 1241 370
Fragments arranged by size:
1241 491 370 85
BtsI GCAGTG_nn'
Cuts at: 0 488 650 701 1436 1506 1516 1900 2187
Size: 488 162 51 735 70 10 384 287
Fragments arranged by size:
735 488 384 287 162 70 51 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1038 1073 2187
Size: 1038 35 1114
Fragments arranged by size:
1114 1038 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 822 2187
Size: 822 1365
EaeI y'GGCC_r
Cuts at: 0 410 2063 2118 2187
Size: 410 1653 55 69
Fragments arranged by size:
1653 410 69 55
EagI C'GGCC_G
Cuts at: 0 2118 2187
Size: 2118 69
EarI CTCTTCn'nnn_
Cuts at: 0 46 155 1960 2187
Size: 46 109 1805 227
Fragments arranged by size:
1805 227 109 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1153 2166 2187
Size: 1153 1013 21
Fragments arranged by size:
1153 1013 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 43 88 577 763 998 1542 1927
Size: 37 6 45 489 186 235 544 385
Cuts at: 1927 2187
Size: 260
Fragments arranged by size:
544 489 385 260 235 186 45 37
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 527 1693 1951 2187
Size: 527 1166 258 236
Fragments arranged by size:
1166 527 258 236
EcoO109I rG'GnC_Cy
Cuts at: 0 120 121 325 581 899 900 1371 1660
Size: 120 1 204 256 318 1 471 289
Cuts at: 1660 2000 2187
Size: 340 187
Fragments arranged by size:
471 340 318 289 256 204 187 120
1 1
FspI TGC'GCA
Cuts at: 0 960 2187
Size: 960 1227
FspAI rTGC'GCAy
Cuts at: 0 960 2187
Size: 960 1227
HaeII r_GCGC'y
Cuts at: 0 168 1723 2103 2184 2187
Size: 168 1555 380 81 3
Fragments arranged by size:
1555 380 168 81 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 154 186 190 222 841 873
Size: 99 32 23 32 4 32 619 32
Cuts at: 873 1145 1177 1471 1503 2187
Size: 272 32 294 32 684
Fragments arranged by size:
684 619 294 272 99 32 32 32
32 32 32 23 4
HincII GTy'rAC
Cuts at: 0 1623 2187
Size: 1623 564
KasI G'GCGC_C
Cuts at: 0 2099 2187
Size: 2099 88
MscI TGG'CCA
Cuts at: 0 412 2065 2187
Size: 412 1653 122
Fragments arranged by size:
1653 412 122
MslI CAynn'nnrTG
Cuts at: 0 929 1094 2089 2187
Size: 929 165 995 98
Fragments arranged by size:
995 929 165 98
MspA1I CmG'CkG
Cuts at: 0 255 783 840 879 1065 1406 2136 2187
Size: 255 528 57 39 186 341 730 51
Fragments arranged by size:
730 528 341 255 186 57 51 39
NaeI GCC'GGC
Cuts at: 0 535 1073 2122 2187
Size: 535 538 1049 65
Fragments arranged by size:
1049 538 535 65
NarI GG'CG_CC
Cuts at: 0 2100 2187
Size: 2100 87
NcoI C'CATG_G
Cuts at: 0 491 576 2187
Size: 491 85 1611
Fragments arranged by size:
1611 491 85
NgoMIV G'CCGG_C
Cuts at: 0 533 1071 2120 2187
Size: 533 538 1049 67
Fragments arranged by size:
1049 538 533 67
NspI r_CATG'y
Cuts at: 0 240 739 2061 2187
Size: 240 499 1322 126
Fragments arranged by size:
1322 499 240 126
PasI CC'CwG_GG
Cuts at: 0 584 2187
Size: 584 1603
PciI A'CATG_T
Cuts at: 0 236 2187
Size: 236 1951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 2187
Size: 1222 32 933
Fragments arranged by size:
1222 933 32
PpuMI rG'GwC_Cy
Cuts at: 0 325 1371 1660 2000 2187
Size: 325 1046 289 340 187
Fragments arranged by size:
1046 340 325 289 187
PspOMI G'GGCC_C
Cuts at: 0 120 580 899 2187
Size: 120 460 319 1288
Fragments arranged by size:
1288 460 319 120
PstI C_TGCA'G
Cuts at: 0 310 657 779 909 1322 2187
Size: 310 347 122 130 413 865
Fragments arranged by size:
865 413 347 310 130 122
PvuII CAG'CTG
Cuts at: 0 255 783 840 2187
Size: 255 528 57 1347
Fragments arranged by size:
1347 528 255 57
SanDI GG'GwC_CC
Cuts at: 0 325 2187
Size: 325 1862
SapI GCTCTTCn'nnn_
Cuts at: 0 155 2187
Size: 155 2032
ScaI AGT'ACT
Cuts at: 0 764 1180 2187
Size: 764 416 1007
Fragments arranged by size:
1007 764 416
SfcI C'TryA_G
Cuts at: 0 306 653 775 905 1318 2187
Size: 306 347 122 130 413 869
Fragments arranged by size:
869 413 347 306 130 122
SfoI GGC'GCC
Cuts at: 0 2101 2187
Size: 2101 86
SmlI C'TyrA_G
Cuts at: 0 827 1863 1948 2187
Size: 827 1036 85 239
Fragments arranged by size:
1036 827 239 85
SphI G_CATG'C
Cuts at: 0 2061 2187
Size: 2061 126
StuI AGG'CCT
Cuts at: 0 38 1679 1851 2187
Size: 38 1641 172 336
Fragments arranged by size:
1641 336 172 38
StyI C'CwwG_G
Cuts at: 0 191 356 413 491 576 1583 1767 1886
Size: 191 165 57 78 85 1007 184 119
Cuts at: 1886 2103 2187
Size: 217 84
Fragments arranged by size:
1007 217 191 184 165 119 85 84
78 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1470 1899 2187
Size: 1470 429 288
Fragments arranged by size:
1470 429 288
TatI w'GTAC_w
Cuts at: 0 762 1178 1474 2187
Size: 762 416 296 713
Fragments arranged by size:
762 713 416 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 586 618 2187
Size: 437 32 117 32 1569
Fragments arranged by size:
1569 437 117 32 32
XbaI T'CTAG_A
Cuts at: 0 1080 2187
Size: 1080 1107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1338 1791 1893 2187
Size: 1338 453 102 294
Fragments arranged by size:
1338 453 294 102
XmnI GAAnn'nnTTC
Cuts at: 0 349 1975 2187
Size: 349 1626 212
Fragments arranged by size:
1626 349 212
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PpiI PpuMI PspOMI
PstI PvuII SanDI SapI ScaI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BcgI BciVI BclI BfrBI BmtI BsaAI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII EcoICRI EcoRI
EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XhoI XmaI ZraI