Restriction map for
pF1KA0266
(Linear) (Six-base) MAPSORT of: KIAA0266.seq Check: 104 from: 1 to: 2298
KIAA0266.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 463 661 2298
Size: 251 212 198 1637
Fragments arranged by size:
1637 251 212 198
AflII C'TTAA_G
Cuts at: 0 886 2298
Size: 886 1412
AflIII A'CryG_T
Cuts at: 0 2272 2298
Size: 2272 26
AleI CACnn'nnGTG
Cuts at: 0 1048 2298
Size: 1048 1250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 2298
Size: 1049 32 1217
Fragments arranged by size:
1217 1049 32
AlwNI CAG_nnn'CTG
Cuts at: 0 527 1130 2298
Size: 527 603 1168
Fragments arranged by size:
1168 603 527
ApoI r'AATT_y
Cuts at: 0 557 1247 1261 1983 2298
Size: 557 690 14 722 315
Fragments arranged by size:
722 690 557 315 14
AvaI C'yCGr_G
Cuts at: 0 686 2298
Size: 686 1612
BamHI G'GATC_C
Cuts at: 0 2107 2298
Size: 2107 191
BanII G_rGCy'C
Cuts at: 0 275 687 693 1301 2298
Size: 275 412 6 608 997
Fragments arranged by size:
997 608 412 275 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 2210 2298
Size: 2210 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1068 1102 2298
Size: 1068 34 1196
Fragments arranged by size:
1196 1068 34
BclI T'GATC_A
Cuts at: 0 1667 2298
Size: 1667 631
BglII A'GATC_T
Cuts at: 0 261 1291 2298
Size: 261 1030 1007
Fragments arranged by size:
1030 1007 261
BlpI GC'TnA_GC
Cuts at: 0 1313 2298
Size: 1313 985
Bme1580I G_kGCm'C
Cuts at: 0 1380 2298
Size: 1380 918
BmgBI CAC'GTC
Cuts at: 0 1866 2275 2298
Size: 1866 409 23
Fragments arranged by size:
1866 409 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 1913 2298
Size: 1913 385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 967 991 2298
Size: 568 399 24 1307
Fragments arranged by size:
1307 568 399 24
Bpu10I CC'TnA_GC
Cuts at: 0 1172 2298
Size: 1172 1126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 289 2208 2298
Size: 99 190 1919 90
Fragments arranged by size:
1919 190 99 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 1757 2298
Size: 1757 541
BsaHI Gr'CG_yC
Cuts at: 0 1935 2298
Size: 1935 363
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 989 1019 1348 1378 1683 1713 2298
Size: 989 30 329 30 305 30 585
Fragments arranged by size:
989 585 329 305 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 1048 1753 1771 2298
Size: 559 489 705 18 527
Fragments arranged by size:
705 559 527 489 18
BseYI C'CCAG_C
Cuts at: 0 249 1542 1795 1886 2298
Size: 249 1293 253 91 412
Fragments arranged by size:
1293 412 253 249 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1116 2298
Size: 1108 8 1182
Fragments arranged by size:
1182 1108 8
BsiHKAI G_wGCw'C
Cuts at: 0 1301 2298
Size: 1301 997
BsmI GAATG_Cn'
Cuts at: 0 394 2298
Size: 394 1904
Bsp1286I G_dGCh'C
Cuts at: 0 275 687 693 1301 1380 2298
Size: 275 412 6 608 79 918
Fragments arranged by size:
918 608 412 275 79 6
BspHI T'CATG_A
Cuts at: 0 1197 2298
Size: 1197 1101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1476 2243 2298
Size: 1476 767 55
Fragments arranged by size:
1476 767 55
BsrDI GCAATG_nn'
Cuts at: 0 497 2298
Size: 497 1801
BstXI CCAn_nnnn'nTGG
Cuts at: 0 645 870 901 2298
Size: 645 225 31 1397
Fragments arranged by size:
1397 645 225 31
BstYI r'GATC_y
Cuts at: 0 261 377 597 1291 2107 2298
Size: 261 116 220 694 816 191
Fragments arranged by size:
816 694 261 220 191 116
Bsu36I CC'TnA_GG
Cuts at: 0 1640 1958 2298
Size: 1640 318 340
Fragments arranged by size:
1640 340 318
BtgI C'CryG_G
Cuts at: 0 614 2298
Size: 614 1684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 320 355 1847 1882 2298
Size: 320 35 1492 35 416
Fragments arranged by size:
1492 416 320 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1695 2239 2298
Size: 1695 544 59
Fragments arranged by size:
1695 544 59
EaeI y'GGCC_r
Cuts at: 0 296 932 977 2298
Size: 296 636 45 1321
Fragments arranged by size:
1321 636 296 45
EarI CTCTTCn'nnn_
Cuts at: 0 644 1025 1523 1532 1748 1825 2298
Size: 644 381 498 9 216 77 473
Fragments arranged by size:
644 498 473 381 216 77 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 186 2298
Size: 186 2112
EcoICRI GAG'CTC
Cuts at: 0 1299 2298
Size: 1299 999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 463 568 661 967 991 2298
Size: 251 212 105 93 306 24 1307
Fragments arranged by size:
1307 306 251 212 105 93 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 550 649 2298
Size: 481 69 99 1649
Fragments arranged by size:
1649 481 99 69
EcoO109I rG'GnC_Cy
Cuts at: 0 414 429 1167 1613 1963 2298
Size: 414 15 738 446 350 335
Fragments arranged by size:
738 446 414 350 335 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 1078 2298
Size: 804 274 1220
Fragments arranged by size:
1220 804 274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 1283 1315 1348 1380 2298
Size: 108 32 1143 32 33 32 918
Fragments arranged by size:
1143 918 108 33 32 32 32
HindIII A'AGCT_T
Cuts at: 0 130 1787 2298
Size: 130 1657 511
Fragments arranged by size:
1657 511 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 2298
Size: 847 1451
MscI TGG'CCA
Cuts at: 0 298 934 979 2298
Size: 298 636 45 1319
Fragments arranged by size:
1319 636 298 45
MslI CAynn'nnrTG
Cuts at: 0 1048 2298
Size: 1048 1250
MspA1I CmG'CkG
Cuts at: 0 465 2298
Size: 465 1833
NcoI C'CATG_G
Cuts at: 0 614 2298
Size: 614 1684
NspI r_CATG'y
Cuts at: 0 517 2298
Size: 517 1781
PasI CC'CwG_GG
Cuts at: 0 477 2298
Size: 477 1821
PfoI T'CCnGG_A
Cuts at: 0 1411 2298
Size: 1411 887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 503 535 2298
Size: 503 32 1763
Fragments arranged by size:
1763 503 32
PpuMI rG'GwC_Cy
Cuts at: 0 414 429 1167 1613 1963 2298
Size: 414 15 738 446 350 335
Fragments arranged by size:
738 446 414 350 335 15
PshAI GACnn'nnGTC
Cuts at: 0 1602 2298
Size: 1602 696
PstI C_TGCA'G
Cuts at: 0 698 2298
Size: 698 1600
PvuII CAG'CTG
Cuts at: 0 465 2298
Size: 465 1833
RsrII CG'GwC_CG
Cuts at: 0 1105 2298
Size: 1105 1193
SacI G_AGCT'C
Cuts at: 0 1301 2298
Size: 1301 997
SanDI GG'GwC_CC
Cuts at: 0 429 2298
Size: 429 1869
SapI GCTCTTCn'nnn_
Cuts at: 0 1523 1532 2298
Size: 1523 9 766
Fragments arranged by size:
1523 766 9
SexAI A'CCwGG_T
Cuts at: 0 11 2298
Size: 11 2287
SfcI C'TryA_G
Cuts at: 0 694 808 1498 1675 2102 2298
Size: 694 114 690 177 427 196
Fragments arranged by size:
694 690 427 196 177 114
SmlI C'TyrA_G
Cuts at: 0 78 268 886 2223 2298
Size: 78 190 618 1337 75
Fragments arranged by size:
1337 618 190 78 75
SpeI A'CTAG_T
Cuts at: 0 51 2298
Size: 51 2247
StuI AGG'CCT
Cuts at: 0 625 2298
Size: 625 1673
StyI C'CwwG_G
Cuts at: 0 614 713 2153 2298
Size: 614 99 1440 145
Fragments arranged by size:
1440 614 145 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1122 2137 2298
Size: 1122 1015 161
Fragments arranged by size:
1122 1015 161
TatI w'GTAC_w
Cuts at: 0 1515 2298
Size: 1515 783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 989 1021 1683 1715 2298
Size: 989 32 662 32 583
Fragments arranged by size:
989 662 583 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 942 2298
Size: 942 1356
Enzymes that do cut:
AcuI AflII AflIII AleI AloI AlwNI ApoI AvaI
BamHI BanII BbsI BcgI BclI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BstXI BstYI Bsu36I BtgI CspCI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NspI PasI
PfoI PpiI PpuMI PshAI PstI PvuII RsrII SacI
SanDI SapI SexAI SfcI SmlI SpeI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BciVI BfrBI BglI BmtI BplI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI DraI DraIII EagI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PsiI
PspOMI PspXI PsrI PvuI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI