Restriction map for
pF1KA0261
(Linear) (Six-base) MAPSORT of: KIAA0261.seq Check: 7306 from: 1 to: 3825
KIAA0261.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 134 3825
Size: 134 3691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 734 3136 3565 3825
Size: 569 165 2402 429 260
Fragments arranged by size:
2402 569 429 260 165
AfeI AGC'GCT
Cuts at: 0 3137 3825
Size: 3137 688
AflIII A'CryG_T
Cuts at: 0 2272 3825
Size: 2272 1553
AhdI GACnn_n'nnGTC
Cuts at: 0 1758 3825
Size: 1758 2067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2142 3825
Size: 2142 1683
AlwNI CAG_nnn'CTG
Cuts at: 0 2400 3825
Size: 2400 1425
ApaI G_GGCC'C
Cuts at: 0 13 3825
Size: 13 3812
ApoI r'AATT_y
Cuts at: 0 310 425 516 765 1182 1534 2194 3736
Size: 310 115 91 249 417 352 660 1542
Cuts at: 3736 3825
Size: 89
Fragments arranged by size:
1542 660 417 352 310 249 115 91
89
AseI AT'TA_AT
Cuts at: 0 742 3825
Size: 742 3083
BanI G'GyrC_C
Cuts at: 0 53 1204 3825
Size: 53 1151 2621
Fragments arranged by size:
2621 1151 53
BanII G_rGCy'C
Cuts at: 0 13 3825
Size: 13 3812
BbeI G_GCGC'C
Cuts at: 0 57 3825
Size: 57 3768
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 1007 1997 2144 3825
Size: 635 372 990 147 1681
Fragments arranged by size:
1681 990 635 372 147
BbvCI CC'TCA_GC
Cuts at: 0 2957 3825
Size: 2957 868
BciVI GTATCCnnnnn_n'
Cuts at: 0 1371 3825
Size: 1371 2454
BclI T'GATC_A
Cuts at: 0 3425 3825
Size: 3425 400
BglII A'GATC_T
Cuts at: 0 2478 3825
Size: 2478 1347
BlpI GC'TnA_GC
Cuts at: 0 2973 3825
Size: 2973 852
Bme1580I G_kGCm'C
Cuts at: 0 13 3825
Size: 13 3812
BmrI ACTGGGnnnn_n'
Cuts at: 0 888 2312 2636 3825
Size: 888 1424 324 1189
Fragments arranged by size:
1424 1189 888 324
BmtI G_CTAG'C
Cuts at: 0 2474 3403 3825
Size: 2474 929 422
Fragments arranged by size:
2474 929 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 482 3825
Size: 8 474 3343
Fragments arranged by size:
3343 474 8
Bpu10I CC'TnA_GC
Cuts at: 0 346 1218 2957 3207 3825
Size: 346 872 1739 250 618
Fragments arranged by size:
1739 872 618 346 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3397 3825
Size: 3397 428
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 3127 3825
Size: 358 2769 698
Fragments arranged by size:
2769 698 358
BsaAI yAC'GTr
Cuts at: 0 2169 3825
Size: 2169 1656
BsaBI GATnn'nnATC
Cuts at: 0 1025 3825
Size: 1025 2800
BsaHI Gr'CG_yC
Cuts at: 0 54 3825
Size: 54 3771
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 245 3559 3562 3825
Size: 245 3314 3 263
Fragments arranged by size:
3314 263 245 3
BseYI C'CCAG_C
Cuts at: 0 2384 3201 3395 3825
Size: 2384 817 194 430
Fragments arranged by size:
2384 817 430 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2036 2102 3385 3825
Size: 2036 66 1283 440
Fragments arranged by size:
2036 1283 440 66
BsiHKAI G_wGCw'C
Cuts at: 0 694 2257 2343 3243 3283 3825
Size: 694 1563 86 900 40 542
Fragments arranged by size:
1563 900 694 542 86 40
BsmI GAATG_Cn'
Cuts at: 0 2333 3825
Size: 2333 1492
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1450 3825
Size: 1450 2375
Bsp1286I G_dGCh'C
Cuts at: 0 13 694 2257 2343 3243 3283 3825
Size: 13 681 1563 86 900 40 542
Fragments arranged by size:
1563 900 681 542 86 40 13
BspHI T'CATG_A
Cuts at: 0 771 1554 3825
Size: 771 783 2271
Fragments arranged by size:
2271 783 771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 3825
Size: 134 3691
BsrBI CCG'CTC
Cuts at: 0 59 183 3387 3825
Size: 59 124 3204 438
Fragments arranged by size:
3204 438 124 59
BsrDI GCAATG_nn'
Cuts at: 0 1743 2363 3819 3825
Size: 1743 620 1456 6
Fragments arranged by size:
1743 1456 620 6
BsrFI r'CCGG_y
Cuts at: 0 41 129 3589 3825
Size: 41 88 3460 236
Fragments arranged by size:
3460 236 88 41
BsrGI T'GTAC_A
Cuts at: 0 1104 2410 2589 3825
Size: 1104 1306 179 1236
Fragments arranged by size:
1306 1236 1104 179
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1465 3825
Size: 1465 2360
BstBI TT'CG_AA
Cuts at: 0 2325 3825
Size: 2325 1500
BstEII G'GTnAC_C
Cuts at: 0 1898 3825
Size: 1898 1927
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 3825
Size: 375 3450
BstYI r'GATC_y
Cuts at: 0 465 943 2457 2478 3825
Size: 465 478 1514 21 1347
Fragments arranged by size:
1514 1347 478 465 21
Bsu36I CC'TnA_GG
Cuts at: 0 3173 3825
Size: 3173 652
BtgI C'CryG_G
Cuts at: 0 167 203 3825
Size: 167 36 3622
Fragments arranged by size:
3622 167 36
BtsI GCAGTG_nn'
Cuts at: 0 1406 3035 3825
Size: 1406 1629 790
Fragments arranged by size:
1629 1406 790
ClaI AT'CG_AT
Cuts at: 0 1065 3825
Size: 1065 2760
DraI TTT'AAA
Cuts at: 0 1059 2367 2688 3825
Size: 1059 1308 321 1137
Fragments arranged by size:
1308 1137 1059 321
EaeI y'GGCC_r
Cuts at: 0 206 3780 3825
Size: 206 3574 45
Fragments arranged by size:
3574 206 45
EarI CTCTTCn'nnn_
Cuts at: 0 388 569 2771 3521 3825
Size: 388 181 2202 750 304
Fragments arranged by size:
2202 750 388 304 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 207 3825
Size: 60 147 3618
Fragments arranged by size:
3618 147 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 482 569 734 3136 3565 3825
Size: 8 474 87 165 2402 429 260
Fragments arranged by size:
2402 474 429 260 165 87 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1258 3172 3825
Size: 1258 1914 653
Fragments arranged by size:
1914 1258 653
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 3825
Size: 9 1 3815
Fragments arranged by size:
3815 9 1
EcoRV GAT'ATC
Cuts at: 0 417 990 3825
Size: 417 573 2835
Fragments arranged by size:
2835 573 417
HaeII r_GCGC'y
Cuts at: 0 57 3139 3825
Size: 57 3082 686
Fragments arranged by size:
3082 686 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 979 1011 1744 1776 2427 2459
Size: 478 32 469 32 733 32 651 32
Cuts at: 2459 2503 2535 3825
Size: 44 32 1290
Fragments arranged by size:
1290 733 651 478 469 44 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 3264 3503 3825
Size: 3264 239 322
Fragments arranged by size:
3264 322 239
KasI G'GCGC_C
Cuts at: 0 53 3825
Size: 53 3772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 551 1290 3750 3825
Size: 353 198 739 2460 75
Fragments arranged by size:
2460 739 353 198 75
MscI TGG'CCA
Cuts at: 0 208 3782 3825
Size: 208 3574 43
Fragments arranged by size:
3574 208 43
MslI CAynn'nnrTG
Cuts at: 0 373 809 2124 3002 3058 3825
Size: 373 436 1315 878 56 767
Fragments arranged by size:
1315 878 767 436 373 56
MspA1I CmG'CkG
Cuts at: 0 169 2416 3825
Size: 169 2247 1409
Fragments arranged by size:
2247 1409 169
NaeI GCC'GGC
Cuts at: 0 131 3591 3825
Size: 131 3460 234
Fragments arranged by size:
3460 234 131
NarI GG'CG_CC
Cuts at: 0 54 3825
Size: 54 3771
NdeI CA'TA_TG
Cuts at: 0 2968 3825
Size: 2968 857
NgoMIV G'CCGG_C
Cuts at: 0 129 3589 3825
Size: 129 3460 236
Fragments arranged by size:
3460 236 129
NheI G'CTAG_C
Cuts at: 0 2470 3399 3825
Size: 2470 929 426
Fragments arranged by size:
2470 929 426
NspI r_CATG'y
Cuts at: 0 3589 3825
Size: 3589 236
PflMI CCAn_nnn'nTGG
Cuts at: 0 1931 3825
Size: 1931 1894
PmlI CAC'GTG
Cuts at: 0 2169 3825
Size: 2169 1656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3551 3583 3825
Size: 3551 32 242
Fragments arranged by size:
3551 242 32
PspOMI G'GGCC_C
Cuts at: 0 9 3825
Size: 9 3816
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1097 1129 3825
Size: 1097 32 2696
Fragments arranged by size:
2696 1097 32
PstI C_TGCA'G
Cuts at: 0 1425 3825
Size: 1425 2400
PvuII CAG'CTG
Cuts at: 0 2416 3825
Size: 2416 1409
SacII CC_GC'GG
Cuts at: 0 170 3825
Size: 170 3655
ScaI AGT'ACT
Cuts at: 0 459 590 825 2233 3825
Size: 459 131 235 1408 1592
Fragments arranged by size:
1592 1408 459 235 131
SfcI C'TryA_G
Cuts at: 0 848 1131 1143 1421 3300 3825
Size: 848 283 12 278 1879 525
Fragments arranged by size:
1879 848 525 283 278 12
SfoI GGC'GCC
Cuts at: 0 55 3825
Size: 55 3770
SgrAI Cr'CCGG_yG
Cuts at: 0 41 3825
Size: 41 3784
SmlI C'TyrA_G
Cuts at: 0 3376 3825
Size: 3376 449
SphI G_CATG'C
Cuts at: 0 3589 3825
Size: 3589 236
SspI AAT'ATT
Cuts at: 0 3811 3825
Size: 3811 14
StuI AGG'CCT
Cuts at: 0 1216 3825
Size: 1216 2609
StyI C'CwwG_G
Cuts at: 0 1170 2372 3825
Size: 1170 1202 1453
Fragments arranged by size:
1453 1202 1170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1917 2698 3486 3632 3825
Size: 1917 781 788 146 193
Fragments arranged by size:
1917 788 781 193 146
TatI w'GTAC_w
Cuts at: 0 457 588 823 1104 2231 2410 2589 2945
Size: 457 131 235 281 1127 179 179 356
Cuts at: 2945 3825
Size: 880
Fragments arranged by size:
1127 880 457 356 281 235 179 179
131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3262 3294 3825
Size: 3262 32 531
Fragments arranged by size:
3262 531 32
Tth111I GACn'n_nGTC
Cuts at: 0 634 3825
Size: 634 3191
XbaI T'CTAG_A
Cuts at: 0 2610 3796 3825
Size: 2610 1186 29
Fragments arranged by size:
2610 1186 29
XmnI GAAnn'nnTTC
Cuts at: 0 2176 2936 3825
Size: 2176 760 889
Fragments arranged by size:
2176 889 760
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI
ApoI AseI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI DraI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NspI PflMI PmlI PpiI PspOMI PsrI PstI PvuII
SacII ScaI SfcI SfoI SgrAI SmlI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AloI
ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BcgI
BfrBI BglI BmgBI BplI BsaWI BsaXI BsiEI BsiWI
BspEI BssHII BssSI BstZ17I BtgZI CspCI DraIII DrdI
EagI EcoICRI EcoRI FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NcoI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PpuMI PshAI PsiI
PspXI PvuI RsrII SacI SalI SanDI SapI SbfI
SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SwaI
XcmI XhoI XmaI ZraI