Restriction map for
pF1KA0254
(Linear) (Six-base) MAPSORT of: KIAA0254.seq Check: 6280 from: 1 to: 2976
KIAA0254.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 503 2976
Size: 503 2473
AccI GT'mk_AC
Cuts at: 0 225 2976
Size: 225 2751
Acc65I G'GTAC_C
Cuts at: 0 350 2976
Size: 350 2626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 641 1277 1530 2237 2623 2976
Size: 203 438 636 253 707 386 353
Fragments arranged by size:
707 636 438 386 353 253 203
AfeI AGC'GCT
Cuts at: 0 2614 2976
Size: 2614 362
AflIII A'CryG_T
Cuts at: 0 140 634 2379 2976
Size: 140 494 1745 597
Fragments arranged by size:
1745 597 494 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1726 1937 2976
Size: 1726 211 1039
Fragments arranged by size:
1726 1039 211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 2976
Size: 230 32 2714
Fragments arranged by size:
2714 230 32
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1230 1270 1719 2709 2739 2976
Size: 769 461 40 449 990 30 237
Fragments arranged by size:
990 769 461 449 237 40 30
ApaLI G'TGCA_C
Cuts at: 0 604 2976
Size: 604 2372
ApoI r'AATT_y
Cuts at: 0 648 1323 2192 2771 2881 2976
Size: 648 675 869 579 110 95
Fragments arranged by size:
869 675 648 579 110 95
AvaI C'yCGr_G
Cuts at: 0 1622 2976
Size: 1622 1354
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1584 1617 2976
Size: 1584 33 1359
Fragments arranged by size:
1584 1359 33
BamHI G'GATC_C
Cuts at: 0 776 2976
Size: 776 2200
BanI G'GyrC_C
Cuts at: 0 350 669 1609 2976
Size: 350 319 940 1367
Fragments arranged by size:
1367 940 350 319
BanII G_rGCy'C
Cuts at: 0 199 1477 1559 2098 2976
Size: 199 1278 82 539 878
Fragments arranged by size:
1278 878 539 199 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 1112 2071 2976
Size: 10 1102 959 905
Fragments arranged by size:
1102 959 905 10
BbvCI CC'TCA_GC
Cuts at: 0 884 1271 2173 2976
Size: 884 387 902 803
Fragments arranged by size:
902 884 803 387
BclI T'GATC_A
Cuts at: 0 749 2976
Size: 749 2227
BglII A'GATC_T
Cuts at: 0 1791 1842 2757 2976
Size: 1791 51 915 219
Fragments arranged by size:
1791 915 219 51
BlpI GC'TnA_GC
Cuts at: 0 1536 2797 2976
Size: 1536 1261 179
Fragments arranged by size:
1536 1261 179
Bme1580I G_kGCm'C
Cuts at: 0 608 674 843 863 1212 2976
Size: 608 66 169 20 349 1764
Fragments arranged by size:
1764 608 349 169 66 20
BmgBI CAC'GTC
Cuts at: 0 2380 2976
Size: 2380 596
BmrI ACTGGGnnnn_n'
Cuts at: 0 608 925 1147 2976
Size: 608 317 222 1829
Fragments arranged by size:
1829 608 317 222
BmtI G_CTAG'C
Cuts at: 0 2162 2976
Size: 2162 814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 932 2976
Size: 932 2044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 183 230 902 962 1138 1151 1426
Size: 20 163 47 672 60 176 13 275
Cuts at: 1426 1798 2183 2230 2836 2976
Size: 372 385 47 606 140
Fragments arranged by size:
672 606 385 372 275 176 163 140
60 47 47 20 13
Bpu10I CC'TnA_GC
Cuts at: 0 884 1271 1561 2173 2976
Size: 884 387 290 612 803
Fragments arranged by size:
884 803 612 387 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 1317 2022 2116 2976
Size: 1317 705 94 860
Fragments arranged by size:
1317 860 705 94
BsaHI Gr'CG_yC
Cuts at: 0 702 2976
Size: 702 2274
BsaWI w'CCGG_w
Cuts at: 0 697 2976
Size: 697 2279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 249 279 2976
Size: 249 30 2697
Fragments arranged by size:
2697 249 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1288 1503 1795 1963 2119 2473 2923 2961
Size: 1288 215 292 168 156 354 450 38
Cuts at: 2961 2976
Size: 15
Fragments arranged by size:
1288 450 354 292 215 168 156 38
15
BseYI C'CCAG_C
Cuts at: 0 180 192 831 2425 2615 2801 2976
Size: 180 12 639 1594 190 186 175
Fragments arranged by size:
1594 639 190 186 180 175 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1975 2605 2641 2976
Size: 1975 630 36 335
Fragments arranged by size:
1975 630 335 36
BsiEI CG_ry'CG
Cuts at: 0 1438 2976
Size: 1438 1538
BsiHKAI G_wGCw'C
Cuts at: 0 608 1477 1631 2976
Size: 608 869 154 1345
Fragments arranged by size:
1345 869 608 154
Bsp1286I G_dGCh'C
Cuts at: 0 199 608 674 843 863 1212 1477 1559
Size: 199 409 66 169 20 349 265 82
Cuts at: 1559 1631 2098 2976
Size: 72 467 878
Fragments arranged by size:
878 467 409 349 265 199 169 82
72 66 20
BspHI T'CATG_A
Cuts at: 0 1160 2976
Size: 1160 1816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 1432 2577 2976
Size: 503 929 1145 399
Fragments arranged by size:
1145 929 503 399
BsrDI GCAATG_nn'
Cuts at: 0 166 738 2976
Size: 166 572 2238
Fragments arranged by size:
2238 572 166
BsrFI r'CCGG_y
Cuts at: 0 1308 2792 2976
Size: 1308 1484 184
Fragments arranged by size:
1484 1308 184
BssSI C'ACGA_G
Cuts at: 0 790 2976
Size: 790 2186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2615 2976
Size: 2615 361
BstYI r'GATC_y
Cuts at: 0 765 776 816 1337 1455 1791 1842 2355
Size: 765 11 40 521 118 336 51 513
Cuts at: 2355 2475 2757 2889 2976
Size: 120 282 132 87
Fragments arranged by size:
765 521 513 336 282 132 120 118
87 51 40 11
Bsu36I CC'TnA_GG
Cuts at: 0 275 2976
Size: 275 2701
BtgI C'CryG_G
Cuts at: 0 2680 2976
Size: 2680 296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2302 2976
Size: 2302 674
BtsI GCAGTG_nn'
Cuts at: 0 524 2262 2976
Size: 524 1738 714
Fragments arranged by size:
1738 714 524
DraIII CAC_nnn'GTG
Cuts at: 0 1734 2209 2976
Size: 1734 475 767
Fragments arranged by size:
1734 767 475
EaeI y'GGCC_r
Cuts at: 0 254 1435 2976
Size: 254 1181 1541
Fragments arranged by size:
1541 1181 254
EagI C'GGCC_G
Cuts at: 0 1435 2976
Size: 1435 1541
EcoICRI GAG'CTC
Cuts at: 0 1475 2976
Size: 1475 1501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 183 203 230 641 902 962 1138
Size: 20 163 20 27 411 261 60 176
Cuts at: 1138 1151 1277 1426 1530 1798 2183 2230 2237
Size: 13 126 149 104 268 385 47 7
Cuts at: 2237 2623 2836 2976
Size: 386 213 140
Fragments arranged by size:
411 386 385 268 261 213 176 163
149 140 126 104 60 47 27 20
20 13 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 2372 2976
Size: 565 1807 604
Fragments arranged by size:
1807 604 565
EcoO109I rG'GnC_Cy
Cuts at: 0 970 1087 1822 2558 2693 2976
Size: 970 117 735 736 135 283
Fragments arranged by size:
970 736 735 283 135 117
EcoRI G'AATT_C
Cuts at: 0 1323 2881 2976
Size: 1323 1558 95
Fragments arranged by size:
1558 1323 95
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 699 2976
Size: 699 2277
HaeII r_GCGC'y
Cuts at: 0 2616 2976
Size: 2616 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 1105 1137 1459 1491 2260 2292
Size: 435 32 638 32 322 32 769 32
Cuts at: 2292 2790 2822 2976
Size: 498 32 154
Fragments arranged by size:
769 638 498 435 322 154 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 138 226 2976
Size: 138 88 2750
Fragments arranged by size:
2750 138 88
KpnI G_GTAC'C
Cuts at: 0 354 2976
Size: 354 2622
MluI A'CGCG_T
Cuts at: 0 634 2976
Size: 634 2342
MscI TGG'CCA
Cuts at: 0 256 2976
Size: 256 2720
MslI CAynn'nnrTG
Cuts at: 0 319 1159 2976
Size: 319 840 1817
Fragments arranged by size:
1817 840 319
MspA1I CmG'CkG
Cuts at: 0 40 291 1128 1706 1719 2976
Size: 40 251 837 578 13 1257
Fragments arranged by size:
1257 837 578 251 40 13
NaeI GCC'GGC
Cuts at: 0 2794 2976
Size: 2794 182
NgoMIV G'CCGG_C
Cuts at: 0 2792 2976
Size: 2792 184
NheI G'CTAG_C
Cuts at: 0 2158 2976
Size: 2158 818
NspI r_CATG'y
Cuts at: 0 731 2532 2976
Size: 731 1801 444
Fragments arranged by size:
1801 731 444
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 1230 2976
Size: 711 519 1746
Fragments arranged by size:
1746 711 519
PfoI T'CCnGG_A
Cuts at: 0 26 416 2246 2439 2813 2876 2976
Size: 26 390 1830 193 374 63 100
Fragments arranged by size:
1830 390 374 193 100 63 26
PpuMI rG'GwC_Cy
Cuts at: 0 1087 1822 2558 2693 2976
Size: 1087 735 736 135 283
Fragments arranged by size:
1087 736 735 283 135
PstI C_TGCA'G
Cuts at: 0 500 531 969 1715 1769 2976
Size: 500 31 438 746 54 1207
Fragments arranged by size:
1207 746 500 438 54 31
PvuII CAG'CTG
Cuts at: 0 40 291 1719 2976
Size: 40 251 1428 1257
Fragments arranged by size:
1428 1257 251 40
SacI G_AGCT'C
Cuts at: 0 1477 2976
Size: 1477 1499
SalI G'TCGA_C
Cuts at: 0 224 2976
Size: 224 2752
SanDI GG'GwC_CC
Cuts at: 0 2558 2976
Size: 2558 418
SexAI A'CCwGG_T
Cuts at: 0 1521 2976
Size: 1521 1455
SfcI C'TryA_G
Cuts at: 0 496 527 965 1004 1069 1711 1765 2976
Size: 496 31 438 39 65 642 54 1211
Fragments arranged by size:
1211 642 496 438 65 54 39 31
StuI AGG'CCT
Cuts at: 0 1304 2976
Size: 1304 1672
StyI C'CwwG_G
Cuts at: 0 806 2862 2976
Size: 806 2056 114
Fragments arranged by size:
2056 806 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1009 1664 2140 2607 2953 2976
Size: 1009 655 476 467 346 23
Fragments arranged by size:
1009 655 476 467 346 23
TatI w'GTAC_w
Cuts at: 0 786 2976
Size: 786 2190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 249 281 1189 1221 2976
Size: 249 32 908 32 1755
Fragments arranged by size:
1755 908 249 32 32
XbaI T'CTAG_A
Cuts at: 0 2020 2976
Size: 2020 956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 681 2286 2379 2976
Size: 408 273 1605 93 597
Fragments arranged by size:
1605 597 408 273 93
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlfI AloI
AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KpnI MluI MscI
MslI MspA1I NaeI NgoMIV NheI NspI PflMI PfoI
PpuMI PstI PvuII SacI SalI SanDI SexAI SfcI
StuI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI ApaI AscI
AseI AsiSI AvrII BbeI BcgI BciVI BfrBI BglI
BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BsrBI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI
DraI DrdI EarI EciI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MmeI NarI NcoI NdeI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XhoI XmaI XmnI ZraI