Restriction map for
pF1KSDA0250
(Linear) (Six-base) MAPSORT of: KIAA0250.seq Check: 1512 from: 1 to: 3273
KIAA0250.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 2125 3273
Size: 2125 1148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 101 1763 1933 2311 3273
Size: 64 37 1662 170 378 962
Fragments arranged by size:
1662 962 378 170 64 37
AhdI GACnn_n'nnGTC
Cuts at: 0 1126 3273
Size: 1126 2147
AleI CACnn'nnGTG
Cuts at: 0 923 3273
Size: 923 2350
AlwNI CAG_nnn'CTG
Cuts at: 0 105 114 993 1741 3273
Size: 105 9 879 748 1532
Fragments arranged by size:
1532 879 748 105 9
ApoI r'AATT_y
Cuts at: 0 763 1593 2464 3273
Size: 763 830 871 809
Fragments arranged by size:
871 830 809 763
AseI AT'TA_AT
Cuts at: 0 1474 3273
Size: 1474 1799
BamHI G'GATC_C
Cuts at: 0 2577 3273
Size: 2577 696
BanI G'GyrC_C
Cuts at: 0 3097 3253 3273
Size: 3097 156 20
Fragments arranged by size:
3097 156 20
BanII G_rGCy'C
Cuts at: 0 811 1561 1903 2633 3273
Size: 811 750 342 730 640
Fragments arranged by size:
811 750 730 640 342
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1490 3273
Size: 359 1131 1783
Fragments arranged by size:
1783 1131 359
BfrBI ATG'CAT
Cuts at: 0 3186 3273
Size: 3186 87
BglII A'GATC_T
Cuts at: 0 2503 3273
Size: 2503 770
BlpI GC'TnA_GC
Cuts at: 0 536 2108 3273
Size: 536 1572 1165
Fragments arranged by size:
1572 1165 536
BmrI ACTGGGnnnn_n'
Cuts at: 0 1484 1788 2481 3273
Size: 1484 304 693 792
Fragments arranged by size:
1484 792 693 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1236 2640 3150 3273
Size: 1236 1404 510 123
Fragments arranged by size:
1404 1236 510 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1718 1838 1952 2948 3226 3273
Size: 1718 120 114 996 278 47
Fragments arranged by size:
1718 996 278 120 114 47
Bpu10I CC'TnA_GC
Cuts at: 0 1902 3273
Size: 1902 1371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1206 1763 3273
Size: 1206 557 1510
Fragments arranged by size:
1510 1206 557
BsaI GGTCTCn'nnnn_
Cuts at: 0 593 1101 1275 1667 2825 3273
Size: 593 508 174 392 1158 448
Fragments arranged by size:
1158 593 508 448 392 174
BsaWI w'CCGG_w
Cuts at: 0 2665 3273
Size: 2665 608
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3199 3229 3273
Size: 3199 30 44
Fragments arranged by size:
3199 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1072 1264 1820 2861 2897 3273
Size: 1072 192 556 1041 36 376
Fragments arranged by size:
1072 1041 556 376 192 36
BseYI C'CCAG_C
Cuts at: 0 69 657 1837 1917 1990 2081 2899 3273
Size: 69 588 1180 80 73 91 818 374
Fragments arranged by size:
1180 818 588 374 91 80 73 69
BsiHKAI G_wGCw'C
Cuts at: 0 2633 3273
Size: 2633 640
BsmI GAATG_Cn'
Cuts at: 0 2597 3273
Size: 2597 676
Bsp1286I G_dGCh'C
Cuts at: 0 811 1561 1903 2633 3273
Size: 811 750 342 730 640
Fragments arranged by size:
811 750 730 640 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2130 3273
Size: 2130 1143
BsrDI GCAATG_nn'
Cuts at: 0 479 635 942 2362 3273
Size: 479 156 307 1420 911
Fragments arranged by size:
1420 911 479 307 156
BsrFI r'CCGG_y
Cuts at: 0 2187 3273
Size: 2187 1086
BsrGI T'GTAC_A
Cuts at: 0 328 3273
Size: 328 2945
BssHII G'CGCG_C
Cuts at: 0 14 3273
Size: 14 3259
BssSI C'ACGA_G
Cuts at: 0 1658 3273
Size: 1658 1615
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 1025 3273
Size: 105 920 2248
Fragments arranged by size:
2248 920 105
BstEII G'GTnAC_C
Cuts at: 0 1315 1632 2549 3273
Size: 1315 317 917 724
Fragments arranged by size:
1315 917 724 317
BstYI r'GATC_y
Cuts at: 0 174 2503 2577 3273
Size: 174 2329 74 696
Fragments arranged by size:
2329 696 174 74
Bsu36I CC'TnA_GG
Cuts at: 0 2273 3273
Size: 2273 1000
BtgI C'CryG_G
Cuts at: 0 768 1100 2420 2815 3017 3111 3122 3273
Size: 768 332 1320 395 202 94 11 151
Fragments arranged by size:
1320 768 395 332 202 151 94 11
BtsI GCAGTG_nn'
Cuts at: 0 456 1148 3273
Size: 456 692 2125
Fragments arranged by size:
2125 692 456
DrdI GACnn_nn'nnGTC
Cuts at: 0 1737 2817 3273
Size: 1737 1080 456
Fragments arranged by size:
1737 1080 456
EarI CTCTTCn'nnn_
Cuts at: 0 818 1085 1190 1205 1838 3273
Size: 818 267 105 15 633 1435
Fragments arranged by size:
1435 818 633 267 105 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1844 3273
Size: 1844 1429
EcoICRI GAG'CTC
Cuts at: 0 2631 3273
Size: 2631 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 101 1718 1763 1838 1933 1952 2311
Size: 64 37 1617 45 75 95 19 359
Cuts at: 2311 2948 3226 3273
Size: 637 278 47
Fragments arranged by size:
1617 637 359 278 95 75 64 47
45 37 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 522 3273
Size: 28 494 2751
Fragments arranged by size:
2751 494 28
EcoO109I rG'GnC_Cy
Cuts at: 0 111 1215 2056 2457 3273
Size: 111 1104 841 401 816
Fragments arranged by size:
1104 841 816 401 111
EcoRI G'AATT_C
Cuts at: 0 2464 3273
Size: 2464 809
HaeII r_GCGC'y
Cuts at: 0 413 3273
Size: 413 2860
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1112 1144 1693 1725 2488 2520 2620 2652
Size: 1112 32 549 32 763 32 100 32
Cuts at: 2652 2660 2692 2976 3008 3028 3060 3273
Size: 8 32 284 32 20 32 213
Fragments arranged by size:
1112 763 549 284 213 100 32 32
32 32 32 32 32 20 8
HindIII A'AGCT_T
Cuts at: 0 860 3273
Size: 860 2413
MfeI C'AATT_G
Cuts at: 0 373 3273
Size: 373 2900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 2935 3273
Size: 961 1974 338
Fragments arranged by size:
1974 961 338
MslI CAynn'nnrTG
Cuts at: 0 640 773 923 1205 3273
Size: 640 133 150 282 2068
Fragments arranged by size:
2068 640 282 150 133
MspA1I CmG'CkG
Cuts at: 0 79 661 1921 2136 2259 3213 3273
Size: 79 582 1260 215 123 954 60
Fragments arranged by size:
1260 954 582 215 123 79 60
NaeI GCC'GGC
Cuts at: 0 2189 3273
Size: 2189 1084
NcoI C'CATG_G
Cuts at: 0 1100 2420 3017 3111 3122 3273
Size: 1100 1320 597 94 11 151
Fragments arranged by size:
1320 1100 597 151 94 11
NdeI CA'TA_TG
Cuts at: 0 1866 3273
Size: 1866 1407
NgoMIV G'CCGG_C
Cuts at: 0 2187 3273
Size: 2187 1086
NsiI A_TGCA'T
Cuts at: 0 3188 3273
Size: 3188 85
NspI r_CATG'y
Cuts at: 0 532 3273
Size: 532 2741
PasI CC'CwG_GG
Cuts at: 0 1124 1561 3273
Size: 1124 437 1712
Fragments arranged by size:
1712 1124 437
PflMI CCAn_nnn'nTGG
Cuts at: 0 1106 3273
Size: 1106 2167
PfoI T'CCnGG_A
Cuts at: 0 3189 3273
Size: 3189 84
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 3273
Size: 161 32 3080
Fragments arranged by size:
3080 161 32
PpuMI rG'GwC_Cy
Cuts at: 0 111 1215 2056 2457 3273
Size: 111 1104 841 401 816
Fragments arranged by size:
1104 841 816 401 111
PsiI TTA'TAA
Cuts at: 0 566 3273
Size: 566 2707
PstI C_TGCA'G
Cuts at: 0 11 110 631 1551 2141 2991 3273
Size: 11 99 521 920 590 850 282
Fragments arranged by size:
920 850 590 521 282 99 11
PvuII CAG'CTG
Cuts at: 0 79 661 1921 2136 2259 3213 3273
Size: 79 582 1260 215 123 954 60
Fragments arranged by size:
1260 954 582 215 123 79 60
SacI G_AGCT'C
Cuts at: 0 2633 3273
Size: 2633 640
SexAI A'CCwGG_T
Cuts at: 0 1979 3273
Size: 1979 1294
SfcI C'TryA_G
Cuts at: 0 7 106 522 627 1039 1382 1547 1955
Size: 7 99 416 105 412 343 165 408
Cuts at: 1955 2137 2484 2987 3273
Size: 182 347 503 286
Fragments arranged by size:
503 416 412 408 347 343 286 182
165 105 99 7
SmlI C'TyrA_G
Cuts at: 0 1221 1778 3273
Size: 1221 557 1495
Fragments arranged by size:
1495 1221 557
SphI G_CATG'C
Cuts at: 0 532 3273
Size: 532 2741
StuI AGG'CCT
Cuts at: 0 754 3273
Size: 754 2519
StyI C'CwwG_G
Cuts at: 0 471 1100 1411 1677 2420 3017 3111 3122
Size: 471 629 311 266 743 597 94 11
Cuts at: 3122 3273
Size: 151
Fragments arranged by size:
743 629 597 471 311 266 151 94
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3010 3273
Size: 3010 263
TatI w'GTAC_w
Cuts at: 0 328 1160 3273
Size: 328 832 2113
Fragments arranged by size:
2113 832 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1967 1999 3273
Size: 1967 32 1274
Fragments arranged by size:
1967 1274 32
XbaI T'CTAG_A
Cuts at: 0 2394 3273
Size: 2394 879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 83 1987 2468 3273
Size: 83 1904 481 805
Fragments arranged by size:
1904 805 481 83
XmnI GAAnn'nnTTC
Cuts at: 0 1195 3273
Size: 1195 2078
Enzymes that do cut:
AccI AcuI AhdI AleI AlwNI ApoI AseI BamHI
BanI BanII BbsI BfrBI BglII BlpI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI DrdI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII MfeI MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PfoI PpiI
PpuMI PsiI PstI PvuII SacI SexAI SfcI SmlI
SphI StuI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BglI Bme1580I
BmgBI BmtI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BstBI BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII EaeI EagI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MscI NarI NheI NotI NruI PacI PciI PmeI
PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XhoI XmaI ZraI