Restriction map for
pF1KA0246
(Linear) (Six-base) MAPSORT of: KIAA0246.seq Check: 6072 from: 1 to: 7710
KIAA0246.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 66 641 910 1082 2504 2806 2896 4324
Size: 66 575 269 172 1422 302 90 1428
Cuts at: 4324 4456 4675 4747 5662 7710
Size: 132 219 72 915 2048
Fragments arranged by size:
2048 1428 1422 915 575 302 269 219
172 132 90 72 66
AatII G_ACGT'C
Cuts at: 0 5752 7710
Size: 5752 1958
AccI GT'mk_AC
Cuts at: 0 1263 1874 2075 3887 7710
Size: 1263 611 201 1812 3823
Fragments arranged by size:
3823 1812 1263 611 201
Acc65I G'GTAC_C
Cuts at: 0 1724 7710
Size: 1724 5986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 1539 2412 2937 3405 4776 5004 5202
Size: 42 1497 873 525 468 1371 228 198
Cuts at: 5202 5251 7433 7710
Size: 49 2182 277
Fragments arranged by size:
2182 1497 1371 873 525 468 277 228
198 49 42
AflII C'TTAA_G
Cuts at: 0 3022 7710
Size: 3022 4688
AflIII A'CryG_T
Cuts at: 0 160 2026 2413 3928 4696 7710
Size: 160 1866 387 1515 768 3014
Fragments arranged by size:
3014 1866 1515 768 387 160
AgeI A'CCGG_T
Cuts at: 0 461 980 7710
Size: 461 519 6730
Fragments arranged by size:
6730 519 461
AleI CACnn'nnGTG
Cuts at: 0 1323 4141 7710
Size: 1323 2818 3569
Fragments arranged by size:
3569 2818 1323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 673 1939 6297 6613 7710
Size: 514 159 1266 4358 316 1097
Fragments arranged by size:
4358 1266 1097 514 316 159
AlwNI CAG_nnn'CTG
Cuts at: 0 81 603 1806 2129 3402 5589 5663 5786
Size: 81 522 1203 323 1273 2187 74 123
Cuts at: 5786 5834 5876 5985 6519 6744 7264 7277 7710
Size: 48 42 109 534 225 520 13 433
Fragments arranged by size:
2187 1273 1203 534 522 520 433 323
225 123 109 81 74 48 42 13
ApaI G_GGCC'C
Cuts at: 0 11 1186 1619 2471 2779 3162 3640 4130
Size: 11 1175 433 852 308 383 478 490
Cuts at: 4130 5443 6047 6396 7284 7710
Size: 1313 604 349 888 426
Fragments arranged by size:
1313 1175 888 852 604 490 478 433
426 383 349 308 11
ApaLI G'TGCA_C
Cuts at: 0 499 6191 6588 7710
Size: 499 5692 397 1122
Fragments arranged by size:
5692 1122 499 397
AvaI C'yCGr_G
Cuts at: 0 11 1849 2779 3106 3362 3911 4748 5131
Size: 11 1838 930 327 256 549 837 383
Cuts at: 5131 5169 6196 7502 7710
Size: 38 1027 1306 208
Fragments arranged by size:
1838 1306 1027 930 837 549 383 327
256 208 38 11
AvrII C'CTAG_G
Cuts at: 0 4261 7710
Size: 4261 3449
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 107 140 7710
Size: 107 33 7570
Fragments arranged by size:
7570 107 33
BamHI G'GATC_C
Cuts at: 0 1509 1781 2246 7694 7710
Size: 1509 272 465 5448 16
Fragments arranged by size:
5448 1509 465 272 16
BanI G'GyrC_C
Cuts at: 0 323 653 1200 1724 2653 3072 3175 3411
Size: 323 330 547 524 929 419 103 236
Cuts at: 3411 3459 3563 3948 4425 5006 6034 6856 7380
Size: 48 104 385 477 581 1028 822 524
Cuts at: 7380 7407 7710
Size: 27 303
Fragments arranged by size:
1028 929 822 581 547 524 524 477
419 385 330 323 303 236 104 103
48 27
BanII G_rGCy'C
Cuts at: 0 11 188 238 696 751 1186 1619 1993
Size: 11 177 50 458 55 435 433 374
Cuts at: 1993 2099 2471 2779 3106 3162 3640 3769 4130
Size: 106 372 308 327 56 478 129 361
Cuts at: 4130 4619 4766 4823 5319 5443 5507 6047 6106
Size: 489 147 57 496 124 64 540 59
Cuts at: 6106 6396 7088 7284 7488 7710
Size: 290 692 196 204 222
Fragments arranged by size:
692 540 496 489 478 458 435 433
374 372 361 327 308 290 222 204
196 177 147 129 124 106 64 59
57 56 55 50 11
BbeI G_GCGC'C
Cuts at: 0 7411 7710
Size: 7411 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 1449 2351 3648 7710
Size: 1449 902 1297 4062
Fragments arranged by size:
4062 1449 1297 902
BbvCI CC'TCA_GC
Cuts at: 0 1126 3340 3794 6677 6722 7237 7710
Size: 1126 2214 454 2883 45 515 473
Fragments arranged by size:
2883 2214 1126 515 473 454 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 967 1001 1920 1954 2780 2814 2823 2857
Size: 967 34 919 34 826 34 9 34
Cuts at: 2857 4261 4295 7710
Size: 1404 34 3415
Fragments arranged by size:
3415 1404 967 919 826 34 34 34
34 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 550 4327 7710
Size: 550 3777 3383
Fragments arranged by size:
3777 3383 550
BclI T'GATC_A
Cuts at: 0 576 7710
Size: 576 7134
BfrBI ATG'CAT
Cuts at: 0 1240 5457 7710
Size: 1240 4217 2253
Fragments arranged by size:
4217 2253 1240
BglI GCCn_nnn'nGGC
Cuts at: 0 53 341 662 911 2408 2475 2678 2858
Size: 53 288 321 249 1497 67 203 180
Cuts at: 2858 2933 3381 4079 4391 4457 5918 6101 6215
Size: 75 448 698 312 66 1461 183 114
Cuts at: 6215 6491 6767 6932 7288 7540 7710
Size: 276 276 165 356 252 170
Fragments arranged by size:
1497 1461 698 448 356 321 312 288
276 276 252 249 203 183 180 170
165 114 75 67 66 53
BglII A'GATC_T
Cuts at: 0 4863 5213 7710
Size: 4863 350 2497
Fragments arranged by size:
4863 2497 350
BlpI GC'TnA_GC
Cuts at: 0 432 3100 7710
Size: 432 2668 4610
Fragments arranged by size:
4610 2668 432
Bme1580I G_kGCm'C
Cuts at: 0 11 503 1186 1619 2413 2421 2471 2656
Size: 11 492 683 433 794 8 50 185
Cuts at: 2656 2779 2929 3077 3162 3180 3377 3464 3503
Size: 123 150 148 85 18 197 87 39
Cuts at: 3503 3640 4130 4158 4261 4749 5009 5443 6047
Size: 137 490 28 103 488 260 434 604
Cuts at: 6047 6195 6396 6592 6861 7284 7503 7710
Size: 148 201 196 269 423 219 207
Fragments arranged by size:
794 683 604 492 490 488 434 433
423 269 260 219 207 201 197 196
185 150 148 148 137 123 103 87
85 50 39 28 18 11 8
BmgBI CAC'GTC
Cuts at: 0 161 1878 2733 7710
Size: 161 1717 855 4977
Fragments arranged by size:
4977 1717 855 161
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 618 729 5154 6146 7710
Size: 321 297 111 4425 992 1564
Fragments arranged by size:
4425 1564 992 321 297 111
BmtI G_CTAG'C
Cuts at: 0 5374 7710
Size: 5374 2336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3606 7710
Size: 3606 4104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1315 1597 2703 3004 3154 3538 3736
Size: 1209 106 282 1106 301 150 384 198
Cuts at: 3736 4780 4996 5611 5692 6181 6402 6541 6619
Size: 1044 216 615 81 489 221 139 78
Cuts at: 6619 7478 7687 7710
Size: 859 209 23
Fragments arranged by size:
1209 1106 1044 859 615 489 384 301
282 221 216 209 198 150 139 106
81 78 23
Bpu10I CC'TnA_GC
Cuts at: 0 1126 3340 3794 4020 6677 6722 7237 7710
Size: 1126 2214 454 226 2657 45 515 473
Fragments arranged by size:
2657 2214 1126 515 473 454 226 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3753 4807 6471 7710
Size: 3753 1054 1664 1239
Fragments arranged by size:
3753 1664 1239 1054
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 1702 2371 4569 5623 7060 7608 7710
Size: 343 1359 669 2198 1054 1437 548 102
Fragments arranged by size:
2198 1437 1359 1054 669 548 343 102
BsaAI yAC'GTr
Cuts at: 0 526 1464 2027 2414 2924 4938 6498 6959
Size: 526 938 563 387 510 2014 1560 461
Cuts at: 6959 7091 7710
Size: 132 619
Fragments arranged by size:
2014 1560 938 619 563 526 510 461
387 132
BsaHI Gr'CG_yC
Cuts at: 0 1454 5303 5749 7408 7710
Size: 1454 3849 446 1659 302
Fragments arranged by size:
3849 1659 1454 446 302
BsaWI w'CCGG_w
Cuts at: 0 461 980 4138 4604 5325 7710
Size: 461 519 3158 466 721 2385
Fragments arranged by size:
3158 2385 721 519 466 461
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1061 1091 2578 2608 3610 3640
Size: 17 30 1014 30 1487 30 1002 30
Cuts at: 3640 4969 4999 5739 5769 7710
Size: 1329 30 740 30 1941
Fragments arranged by size:
1941 1487 1329 1014 1002 740 30 30
30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 1203 1212 1840 1981 3202 3205 5187
Size: 10 1193 9 628 141 1221 3 1982
Cuts at: 5187 6538 7684 7710
Size: 1351 1146 26
Fragments arranged by size:
1982 1351 1221 1193 1146 628 141 26
10 9 3
BseYI C'CCAG_C
Cuts at: 0 228 643 850 1250 1406 1842 2483 2805
Size: 228 415 207 400 156 436 641 322
Cuts at: 2805 3204 3245 4113 4556 5797 5869 5923 6004
Size: 399 41 868 443 1241 72 54 81
Cuts at: 6004 6737 6744 6881 7046 7710
Size: 733 7 137 165 664
Fragments arranged by size:
1241 868 733 664 641 443 436 415
400 399 322 228 207 165 156 137
81 72 54 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 307 510 882 1050 1108 2169 2559
Size: 114 193 203 372 168 58 1061 390
Cuts at: 2559 2649 2853 2904 3301 3807 3825 4119 4312
Size: 90 204 51 397 506 18 294 193
Cuts at: 4312 5086 5125 5557 5746 6051 6233 6252 6390
Size: 774 39 432 189 305 182 19 138
Cuts at: 6390 6583 7710
Size: 193 1127
Fragments arranged by size:
1127 1061 774 506 432 397 390 372
305 294 204 203 193 193 193 189
182 168 138 114 90 58 51 39
19 18
BsiEI CG_ry'CG
Cuts at: 0 981 1671 3054 4304 7710
Size: 981 690 1383 1250 3406
Fragments arranged by size:
3406 1383 1250 981 690
BsiHKAI G_wGCw'C
Cuts at: 0 188 503 631 742 4021 4619 4766 4823
Size: 188 315 128 111 3279 598 147 57
Cuts at: 4823 5319 5507 5613 6030 6195 6314 6440 6457
Size: 496 188 106 417 165 119 126 17
Cuts at: 6457 6592 7088 7464 7488 7710
Size: 135 496 376 24 222
Fragments arranged by size:
3279 598 496 496 417 376 315 222
188 188 165 147 135 128 126 119
111 106 57 24 17
BsmI GAATG_Cn'
Cuts at: 0 337 691 1242 2631 3291 7710
Size: 337 354 551 1389 660 4419
Fragments arranged by size:
4419 1389 660 551 354 337
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1215 7710
Size: 1215 6495
Bsp1286I G_dGCh'C
Cuts at: 0 11 188 238 503 631 696 742 751
Size: 11 177 50 265 128 65 46 9
Cuts at: 751 1186 1619 1993 2099 2413 2421 2471 2656
Size: 435 433 374 106 314 8 50 185
Cuts at: 2656 2779 2929 3077 3106 3162 3180 3377 3464
Size: 123 150 148 29 56 18 197 87
Cuts at: 3464 3503 3640 3769 4021 4130 4158 4261 4619
Size: 39 137 129 252 109 28 103 358
Cuts at: 4619 4749 4766 4823 5009 5319 5443 5507 5613
Size: 130 17 57 186 310 124 64 106
Cuts at: 5613 6030 6047 6106 6195 6314 6396 6440 6457
Size: 417 17 59 89 119 82 44 17
Cuts at: 6457 6592 6861 7088 7284 7464 7488 7503 7710
Size: 135 269 227 196 180 24 15 207
Fragments arranged by size:
435 433 417 374 358 314 310 269
265 252 227 207 197 196 186 185
180 177 150 148 137 135 130 129
128 124 123 119 109 106 106 103
89 87 82 65 64 59 57 56
50 50 46 44 39 29 28 24
18 17 17 17 15 11 9 8
BspEI T'CCGG_A
Cuts at: 0 4604 5325 7710
Size: 4604 721 2385
Fragments arranged by size:
4604 2385 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 382 641 910 1082 2504 2806 2896
Size: 66 316 259 269 172 1422 302 90
Cuts at: 2896 4324 4456 4551 4618 4675 4711 4747 4979
Size: 1428 132 95 67 57 36 36 232
Cuts at: 4979 5435 5662 6361 6605 7710
Size: 456 227 699 244 1105
Fragments arranged by size:
1428 1422 1105 699 456 316 302 269
259 244 232 227 172 132 95 90
67 66 57 36 36
BsrBI CCG'CTC
Cuts at: 0 254 3512 3731 7710
Size: 254 3258 219 3979
Fragments arranged by size:
3979 3258 254 219
BsrDI GCAATG_nn'
Cuts at: 0 366 1647 2262 2341 2868 4350 5409 6834
Size: 366 1281 615 79 527 1482 1059 1425
Cuts at: 6834 6969 7710
Size: 135 741
Fragments arranged by size:
1482 1425 1281 1059 741 615 527 366
135 79
BsrFI r'CCGG_y
Cuts at: 0 461 905 980 2435 3031 5239 6457 7710
Size: 461 444 75 1455 596 2208 1218 1253
Fragments arranged by size:
2208 1455 1253 1218 596 461 444 75
BsrGI T'GTAC_A
Cuts at: 0 6265 7710
Size: 6265 1445
BssHII G'CGCG_C
Cuts at: 0 5059 7710
Size: 5059 2651
BssSI C'ACGA_G
Cuts at: 0 135 1081 4075 7710
Size: 135 946 2994 3635
Fragments arranged by size:
3635 2994 946 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 137 285 686 1246 2297 2591 3390 5013
Size: 137 148 401 560 1051 294 799 1623
Cuts at: 5013 5834 5900 6404 7710
Size: 821 66 504 1306
Fragments arranged by size:
1623 1306 1051 821 799 560 504 401
294 148 137 66
BstBI TT'CG_AA
Cuts at: 0 5448 7710
Size: 5448 2262
BstEII G'GTnAC_C
Cuts at: 0 999 7710
Size: 999 6711
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 2257 2478 4387 4942 7710
Size: 796 1461 221 1909 555 2768
Fragments arranged by size:
2768 1909 1461 796 555 221
BstYI r'GATC_y
Cuts at: 0 1509 1532 1781 2246 3941 4863 5213 7694
Size: 1509 23 249 465 1695 922 350 2481
Cuts at: 7694 7710
Size: 16
Fragments arranged by size:
2481 1695 1509 922 465 350 249 23
16
Bsu36I CC'TnA_GG
Cuts at: 0 168 3680 4768 5687 5714 7710
Size: 168 3512 1088 919 27 1996
Fragments arranged by size:
3512 1996 1088 919 168 27
BtgI C'CryG_G
Cuts at: 0 366 426 516 789 1136 1740 1790 2361
Size: 366 60 90 273 347 604 50 571
Cuts at: 2361 2453 2607 2874 3450 3593 4026 4086 4158
Size: 92 154 267 576 143 433 60 72
Cuts at: 4158 4380 4386 4512 4721 4991 5943 6420 7514
Size: 222 6 126 209 270 952 477 1094
Cuts at: 7514 7573 7710
Size: 59 137
Fragments arranged by size:
1094 952 604 576 571 477 433 366
347 273 270 267 222 209 154 143
137 126 92 90 72 60 60 59
50 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 809 1260 2277 4844 4897 6518 7710
Size: 809 451 1017 2567 53 1621 1192
Fragments arranged by size:
2567 1621 1192 1017 809 451 53
BtsI GCAGTG_nn'
Cuts at: 0 687 724 771 1956 2197 2244 2394 2487
Size: 687 37 47 1185 241 47 150 93
Cuts at: 2487 2661 2903 3479 4039 4068 5011 5901 5979
Size: 174 242 576 560 29 943 890 78
Cuts at: 5979 5986 6312 6520 6788 6992 7406 7462 7660
Size: 7 326 208 268 204 414 56 198
Cuts at: 7660 7710
Size: 50
Fragments arranged by size:
1185 943 890 687 576 560 414 326
268 242 241 208 204 198 174 150
93 78 56 50 47 47 37 29
7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 822 857 3246 3281 3651 3686 5827 5862
Size: 822 35 2389 35 370 35 2141 35
Cuts at: 5862 7710
Size: 1848
Fragments arranged by size:
2389 2141 1848 822 370 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 137 507 626 1086 2192 3570 3983 4722
Size: 137 370 119 460 1106 1378 413 739
Cuts at: 4722 6148 6190 6801 7710
Size: 1426 42 611 909
Fragments arranged by size:
1426 1378 1106 909 739 611 460 413
370 137 119 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 3058 6515 7710
Size: 3058 3457 1195
Fragments arranged by size:
3457 3058 1195
EaeI y'GGCC_r
Cuts at: 0 143 363 709 907 1139 1337 1668 1743
Size: 143 220 346 198 232 198 331 75
Cuts at: 1743 1883 2433 2694 2882 3221 3672 3726 4301
Size: 140 550 261 188 339 451 54 575
Cuts at: 4301 4383 4686 5237 6078 6255 6414 6776 7364
Size: 82 303 551 841 177 159 362 588
Cuts at: 7364 7478 7710
Size: 114 232
Fragments arranged by size:
841 588 575 551 550 451 362 346
339 331 303 261 232 232 220 198
198 188 177 159 143 140 114 82
75 54
EagI C'GGCC_G
Cuts at: 0 1668 4301 7710
Size: 1668 2633 3409
Fragments arranged by size:
3409 2633 1668
EarI CTCTTCn'nnn_
Cuts at: 0 1690 3187 3433 3871 7108 7710
Size: 1690 1497 246 438 3237 602
Fragments arranged by size:
3237 1690 1497 602 438 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4789 6832 7558 7710
Size: 4789 2043 726 152
Fragments arranged by size:
4789 2043 726 152
EcoICRI GAG'CTC
Cuts at: 0 186 4617 4764 4821 5317 5505 7086 7486
Size: 186 4431 147 57 496 188 1581 400
Cuts at: 7486 7710
Size: 224
Fragments arranged by size:
4431 1581 496 400 224 188 186 147
57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 1209 1315 1539 1597 2412 2703 2937
Size: 42 1167 106 224 58 815 291 234
Cuts at: 2937 3004 3154 3405 3538 3736 4776 4780 4996
Size: 67 150 251 133 198 1040 4 216
Cuts at: 4996 5004 5202 5251 5611 5692 6181 6402 6541
Size: 8 198 49 360 81 489 221 139
Cuts at: 6541 6619 7433 7478 7687 7710
Size: 78 814 45 209 23
Fragments arranged by size:
1167 1040 815 814 489 360 291 251
234 224 221 216 209 198 198 150
139 133 106 81 78 67 58 49
45 42 23 8 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3184 3343 3580 3607 4882 5230 6283 7710
Size: 3184 159 237 27 1275 348 1053 1427
Fragments arranged by size:
3184 1427 1275 1053 348 237 159 27
EcoO109I rG'GnC_Cy
Cuts at: 0 7 8 15 371 401 819 1183 1307
Size: 7 1 7 356 30 418 364 124
Cuts at: 1307 1488 1499 1616 1803 1846 1857 2261 2645
Size: 181 11 117 187 43 11 404 384
Cuts at: 2645 2776 3158 3585 3637 3706 3720 3753 3798
Size: 131 382 427 52 69 14 33 45
Cuts at: 3798 4221 4968 5276 5462 5813 6044 6093 7261
Size: 423 747 308 186 351 231 49 1168
Cuts at: 7261 7281 7710
Size: 20 429
Fragments arranged by size:
1168 747 429 427 423 418 404 384
382 364 356 351 308 231 187 186
181 131 124 117 69 52 49 45
43 33 30 20 14 11 11 7
7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2826 7119 7710
Size: 2826 4293 591
Fragments arranged by size:
4293 2826 591
FseI GG_CCGG'CC
Cuts at: 0 5243 7710
Size: 5243 2467
FspI TGC'GCA
Cuts at: 0 4910 7710
Size: 4910 2800
FspAI rTGC'GCAy
Cuts at: 0 4910 7710
Size: 4910 2800
HaeII r_GCGC'y
Cuts at: 0 347 5140 5730 6477 7411 7710
Size: 347 4793 590 747 934 299
Fragments arranged by size:
4793 934 747 590 347 299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 792 824 1061 1093 1256 1288
Size: 466 32 294 32 237 32 163 32
Cuts at: 1288 1520 1552 2554 2586 4615 4647 5739 5771
Size: 232 32 1002 32 2029 32 1092 32
Cuts at: 5771 7552 7584 7710
Size: 1781 32 126
Fragments arranged by size:
2029 1781 1092 1002 466 294 237 232
163 126 32 32 32 32 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 5906 7710
Size: 5906 1804
KasI G'GCGC_C
Cuts at: 0 7407 7710
Size: 7407 303
KpnI G_GTAC'C
Cuts at: 0 1728 7710
Size: 1728 5982
MfeI C'AATT_G
Cuts at: 0 2232 5844 7710
Size: 2232 3612 1866
Fragments arranged by size:
3612 2232 1866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1759 2234 6509 7199 7710
Size: 1759 475 4275 690 511
Fragments arranged by size:
4275 1759 690 511 475
MscI TGG'CCA
Cuts at: 0 365 711 1141 2884 3223 3674 4688 6416
Size: 365 346 430 1743 339 451 1014 1728
Cuts at: 6416 6778 7366 7710
Size: 362 588 344
Fragments arranged by size:
1743 1728 1014 588 451 430 365 362
346 344 339
MslI CAynn'nnrTG
Cuts at: 0 794 1323 2498 2753 3496 4141 4385 4849
Size: 794 529 1175 255 743 645 244 464
Cuts at: 4849 6578 7710
Size: 1729 1132
Fragments arranged by size:
1729 1175 1132 794 743 645 529 464
255 244
MspA1I CmG'CkG
Cuts at: 0 228 428 494 647 1006 1022 1125 1180
Size: 228 200 66 153 359 16 103 55
Cuts at: 1180 1693 1749 1863 2507 2913 2957 3099 3402
Size: 513 56 114 644 406 44 142 303
Cuts at: 3402 3839 3858 4058 4229 4437 4499 4574 4592
Size: 437 19 200 171 208 62 75 18
Cuts at: 4592 4920 5222 5541 5586 5760 5873 6065 6083
Size: 328 302 319 45 174 113 192 18
Cuts at: 6083 6224 6422 6548 6647 6744 7050 7456 7710
Size: 141 198 126 99 97 306 406 254
Fragments arranged by size:
644 513 437 406 406 359 328 319
306 303 302 254 228 208 200 200
198 192 174 171 153 142 141 126
114 113 103 99 97 75 66 62
56 55 45 44 19 18 18 16
NaeI GCC'GGC
Cuts at: 0 907 3033 5241 7710
Size: 907 2126 2208 2469
Fragments arranged by size:
2469 2208 2126 907
NarI GG'CG_CC
Cuts at: 0 7408 7710
Size: 7408 302
NcoI C'CATG_G
Cuts at: 0 516 789 1790 2361 2453 3450 3593 4026
Size: 516 273 1001 571 92 997 143 433
Cuts at: 4026 4086 4158 4386 7514 7710
Size: 60 72 228 3128 196
Fragments arranged by size:
3128 1001 997 571 516 433 273 228
196 143 92 72 60
NdeI CA'TA_TG
Cuts at: 0 4659 7710
Size: 4659 3051
NgoMIV G'CCGG_C
Cuts at: 0 905 3031 5239 7710
Size: 905 2126 2208 2471
Fragments arranged by size:
2471 2208 2126 905
NheI G'CTAG_C
Cuts at: 0 5370 7710
Size: 5370 2340
NsiI A_TGCA'T
Cuts at: 0 1242 5459 7710
Size: 1242 4217 2251
Fragments arranged by size:
4217 2251 1242
NspI r_CATG'y
Cuts at: 0 1908 2503 2672 3932 4445 4700 4783 6689
Size: 1908 595 169 1260 513 255 83 1906
Cuts at: 6689 7171 7710
Size: 482 539
Fragments arranged by size:
1908 1906 1260 595 539 513 482 255
169 83
PasI CC'CwG_GG
Cuts at: 0 337 1504 2641 2649 3525 3997 5196 7294
Size: 337 1167 1137 8 876 472 1199 2098
Cuts at: 7294 7710
Size: 416
Fragments arranged by size:
2098 1199 1167 1137 876 472 416 337
8
PciI A'CATG_T
Cuts at: 0 3928 4696 7710
Size: 3928 768 3014
Fragments arranged by size:
3928 3014 768
PflMI CCAn_nnn'nTGG
Cuts at: 0 361 1806 1908 2389 2880 3245 3456 4722
Size: 361 1445 102 481 491 365 211 1266
Cuts at: 4722 5589 5804 5823 5861 6744 6801 7362 7710
Size: 867 215 19 38 883 57 561 348
Fragments arranged by size:
1445 1266 883 867 561 491 481 365
361 348 215 211 102 57 38 19
PfoI T'CCnGG_A
Cuts at: 0 188 1225 1292 1574 1601 2975 3431 4619
Size: 188 1037 67 282 27 1374 456 1188
Cuts at: 4619 4805 6605 7064 7073 7710
Size: 186 1800 459 9 637
Fragments arranged by size:
1800 1374 1188 1037 637 459 456 282
188 186 67 27 9
PmlI CAC'GTG
Cuts at: 0 526 1464 2027 2414 2924 4938 6959 7710
Size: 526 938 563 387 510 2014 2021 751
Fragments arranged by size:
2021 2014 938 751 563 526 510 387
PpuMI rG'GwC_Cy
Cuts at: 0 371 401 819 1307 1499 1803 1846 1857
Size: 371 30 418 488 192 304 43 11
Cuts at: 1857 2645 3753 4968 5462 7710
Size: 788 1108 1215 494 2248
Fragments arranged by size:
2248 1215 1108 788 494 488 418 371
304 192 43 30 11
PspOMI G'GGCC_C
Cuts at: 0 7 1182 1615 2467 2775 3158 3636 4126
Size: 7 1175 433 852 308 383 478 490
Cuts at: 4126 5439 6043 6392 7280 7710
Size: 1313 604 349 888 430
Fragments arranged by size:
1313 1175 888 852 604 490 478 433
430 383 349 308 7
PstI C_TGCA'G
Cuts at: 0 262 652 1124 1712 1868 1963 2767 3090
Size: 262 390 472 588 156 95 804 323
Cuts at: 3090 3131 3398 3446 3863 4573 5485 5659 6170
Size: 41 267 48 417 710 912 174 511
Cuts at: 6170 6307 6433 6515 7710
Size: 137 126 82 1195
Fragments arranged by size:
1195 912 804 710 588 511 472 417
390 323 267 262 174 156 137 126
95 82 48 41
PvuII CAG'CTG
Cuts at: 0 228 494 647 1022 1125 1749 2957 3099
Size: 228 266 153 375 103 624 1208 142
Cuts at: 3099 3402 3858 4499 4574 4920 5586 5873 6224
Size: 303 456 641 75 346 666 287 351
Cuts at: 6224 6744 7710
Size: 520 966
Fragments arranged by size:
1208 966 666 641 624 520 456 375
351 346 303 287 266 228 153 142
103 75
RsrII CG'GwC_CG
Cuts at: 0 2604 7710
Size: 2604 5106
SacI G_AGCT'C
Cuts at: 0 188 4619 4766 4823 5319 5507 7088 7488
Size: 188 4431 147 57 496 188 1581 400
Cuts at: 7488 7710
Size: 222
Fragments arranged by size:
4431 1581 496 400 222 188 188 147
57
SacII CC_GC'GG
Cuts at: 0 429 6423 7710
Size: 429 5994 1287
Fragments arranged by size:
5994 1287 429
SanDI GG'GwC_CC
Cuts at: 0 1499 1846 1857 2645 5462 7710
Size: 1499 347 11 788 2817 2248
Fragments arranged by size:
2817 2248 1499 788 347 11
SapI GCTCTTCn'nnn_
Cuts at: 0 3871 7710
Size: 3871 3839
SexAI A'CCwGG_T
Cuts at: 0 1799 3149 7710
Size: 1799 1350 4561
Fragments arranged by size:
4561 1799 1350
SfcI C'TryA_G
Cuts at: 0 258 648 1120 1260 1708 1864 1959 2763
Size: 258 390 472 140 448 156 95 804
Cuts at: 2763 3086 3127 3394 3442 3859 4569 5481 5655
Size: 323 41 267 48 417 710 912 174
Cuts at: 5655 6166 6303 6321 6429 6511 7710
Size: 511 137 18 108 82 1199
Fragments arranged by size:
1199 912 804 710 511 472 448 417
390 323 267 258 174 156 140 137
108 95 82 48 41 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 7288 7710
Size: 7288 422
SfoI GGC'GCC
Cuts at: 0 7409 7710
Size: 7409 301
SmaI CCC'GGG
Cuts at: 0 13 2781 3913 7710
Size: 13 2768 1132 3797
Fragments arranged by size:
3797 2768 1132 13
SmlI C'TyrA_G
Cuts at: 0 3022 3768 4822 6450 7710
Size: 3022 746 1054 1628 1260
Fragments arranged by size:
3022 1628 1260 1054 746
SnaBI TAC'GTA
Cuts at: 0 6498 7710
Size: 6498 1212
SphI G_CATG'C
Cuts at: 0 2503 4783 6689 7171 7710
Size: 2503 2280 1906 482 539
Fragments arranged by size:
2503 2280 1906 539 482
StuI AGG'CCT
Cuts at: 0 295 892 914 1552 5338 5413 5719 5822
Size: 295 597 22 638 3786 75 306 103
Cuts at: 5822 7235 7710
Size: 1413 475
Fragments arranged by size:
3786 1413 638 597 475 306 295 103
75 22
StyI C'CwwG_G
Cuts at: 0 516 752 789 815 837 1772 1790 2063
Size: 516 236 37 26 22 935 18 273
Cuts at: 2063 2361 2453 3133 3450 3593 4026 4086 4158
Size: 298 92 680 317 143 433 60 72
Cuts at: 4158 4261 4386 4418 5414 5604 5817 6096 7514
Size: 103 125 32 996 190 213 279 1418
Cuts at: 7514 7710
Size: 196
Fragments arranged by size:
1418 996 935 680 516 433 317 298
279 273 236 213 196 190 143 125
103 92 72 60 37 32 26 22
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 637 2671 3128 3196 3267 3885 5024 5503
Size: 637 2034 457 68 71 618 1139 479
Cuts at: 5503 6844 6931 7060 7702 7710
Size: 1341 87 129 642 8
Fragments arranged by size:
2034 1341 1139 642 637 618 479 457
129 87 71 68 8
TatI w'GTAC_w
Cuts at: 0 222 1391 6265 7710
Size: 222 1169 4874 1445
Fragments arranged by size:
4874 1445 1169 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 2178 2210 3610 3642 3867 3899
Size: 17 32 2129 32 1400 32 225 32
Cuts at: 3899 6000 6032 7091 7123 7710
Size: 2101 32 1059 32 587
Fragments arranged by size:
2129 2101 1400 1059 587 225 32 32
32 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 0 5116 7710
Size: 5116 2594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 334 523 1468 2605 3297 3513 5911 6292
Size: 334 189 945 1137 692 216 2398 381
Cuts at: 6292 7374 7596 7710
Size: 1082 222 114
Fragments arranged by size:
2398 1137 1082 945 692 381 334 222
216 189 114
XmaI C'CCGG_G
Cuts at: 0 11 2779 3911 7710
Size: 11 2768 1132 3799
Fragments arranged by size:
3799 2768 1132 11
ZraI GAC'GTC
Cuts at: 0 5750 7710
Size: 5750 1960
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
FspI FspAI HaeII Hin4I HindIII KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII RsrII SacI SacII
SanDI SapI SexAI SfcI SfiI SfoI SmaI SmlI
SnaBI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AhdI AloI ApoI AscI AseI AsiSI
BsaBI BsiWI BspHI BstZ17I ClaI DraI EcoRI EcoRV
HincII HpaI MluI NotI NruI PacI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI SalI SbfI ScaI
SgfI SgrAI SpeI SrfI SspI SwaI XbaI XhoI
XmnI