Restriction map for
pF1KA0240
(Linear) (Six-base) MAPSORT of: KIAA0240.seq Check: 8120 from: 1 to: 3237
KIAA0240.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2034 3237
Size: 2034 1203
AccI GT'mk_AC
Cuts at: 0 1780 2444 3237
Size: 1780 664 793
Fragments arranged by size:
1780 793 664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1776 2255 2774 2803 3237
Size: 301 1475 479 519 29 434
Fragments arranged by size:
1475 519 479 434 301 29
AflIII A'CryG_T
Cuts at: 0 570 1073 2210 2899 3237
Size: 570 503 1137 689 338
Fragments arranged by size:
1137 689 570 503 338
AgeI A'CCGG_T
Cuts at: 0 2599 3237
Size: 2599 638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1448 1954 2572 3237
Size: 1448 506 618 665
Fragments arranged by size:
1448 665 618 506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 792 793 824 825 3237
Size: 792 1 31 1 2412
Fragments arranged by size:
2412 792 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 486 2196 3237
Size: 486 1710 1041
Fragments arranged by size:
1710 1041 486
ApaI G_GGCC'C
Cuts at: 0 215 3237
Size: 215 3022
ApoI r'AATT_y
Cuts at: 0 135 855 912 995 2263 2713 2934 3237
Size: 135 720 57 83 1268 450 221 303
Fragments arranged by size:
1268 720 450 303 221 135 83 57
AseI AT'TA_AT
Cuts at: 0 683 932 3237
Size: 683 249 2305
Fragments arranged by size:
2305 683 249
AvaI C'yCGr_G
Cuts at: 0 265 2420 3237
Size: 265 2155 817
Fragments arranged by size:
2155 817 265
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1718 1751 3237
Size: 1718 33 1486
Fragments arranged by size:
1718 1486 33
BanI G'GyrC_C
Cuts at: 0 2816 3237
Size: 2816 421
BanII G_rGCy'C
Cuts at: 0 215 758 1057 1925 2224 3237
Size: 215 543 299 868 299 1013
Fragments arranged by size:
1013 868 543 299 299 215
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 1569 1750 2246 3237
Size: 263 1306 181 496 991
Fragments arranged by size:
1306 991 496 263 181
BbvCI CC'TCA_GC
Cuts at: 0 1475 3237
Size: 1475 1762
BciVI GTATCCnnnnn_n'
Cuts at: 0 3235 3237
Size: 3235 2
BglII A'GATC_T
Cuts at: 0 2997 3237
Size: 2997 240
BlpI GC'TnA_GC
Cuts at: 0 416 1632 3237
Size: 416 1216 1605
Fragments arranged by size:
1605 1216 416
Bme1580I G_kGCm'C
Cuts at: 0 215 2819 3237
Size: 215 2604 418
Fragments arranged by size:
2604 418 215
BmgBI CAC'GTC
Cuts at: 0 1248 2750 2900 3237
Size: 1248 1502 150 337
Fragments arranged by size:
1502 1248 337 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 209 1151 3237
Size: 209 942 2086
Fragments arranged by size:
2086 942 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2795 2954 3237
Size: 2795 159 283
Fragments arranged by size:
2795 283 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1715 2098 2986 3237
Size: 229 1486 383 888 251
Fragments arranged by size:
1486 888 383 251 229
Bpu10I CC'TnA_GC
Cuts at: 0 1354 1475 3237
Size: 1354 121 1762
Fragments arranged by size:
1762 1354 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1947 2265 3237
Size: 1947 318 972
Fragments arranged by size:
1947 972 318
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 2579 2664 3237
Size: 35 2544 85 573
Fragments arranged by size:
2544 573 85 35
BsaAI yAC'GTr
Cuts at: 0 1011 2211 3237
Size: 1011 1200 1026
Fragments arranged by size:
1200 1026 1011
BsaWI w'CCGG_w
Cuts at: 0 2599 2819 3237
Size: 2599 220 418
Fragments arranged by size:
2599 418 220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2884 2914 3237
Size: 2884 30 323
Fragments arranged by size:
2884 323 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 1463 3237
Size: 266 1197 1774
Fragments arranged by size:
1774 1197 266
BseYI C'CCAG_C
Cuts at: 0 589 1715 1817 2990 3237
Size: 589 1126 102 1173 247
Fragments arranged by size:
1173 1126 589 247 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2062 2755 3237
Size: 2062 693 482
Fragments arranged by size:
2062 693 482
BsiHKAI G_wGCw'C
Cuts at: 0 1933 2709 2749 2800 3237
Size: 1933 776 40 51 437
Fragments arranged by size:
1933 776 437 51 40
BsmI GAATG_Cn'
Cuts at: 0 2698 3237
Size: 2698 539
Bsp1286I G_dGCh'C
Cuts at: 0 215 758 1057 1925 1933 2224 2709 2749
Size: 215 543 299 868 8 291 485 40
Cuts at: 2749 2800 2819 3237
Size: 51 19 418
Fragments arranged by size:
868 543 485 418 299 291 215 51
40 19 8
BspHI T'CATG_A
Cuts at: 0 2502 2622 2969 3237
Size: 2502 120 347 268
Fragments arranged by size:
2502 347 268 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2034 3237
Size: 2034 1203
BsrDI GCAATG_nn'
Cuts at: 0 99 3237
Size: 99 3138
BsrFI r'CCGG_y
Cuts at: 0 2599 3237
Size: 2599 638
BsrGI T'GTAC_A
Cuts at: 0 549 3237
Size: 549 2688
BssSI C'ACGA_G
Cuts at: 0 18 3237
Size: 18 3219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 529 3237
Size: 529 2708
BstYI r'GATC_y
Cuts at: 0 697 1022 1913 2997 3237
Size: 697 325 891 1084 240
Fragments arranged by size:
1084 891 697 325 240
Bsu36I CC'TnA_GG
Cuts at: 0 1900 1980 2450 2810 3237
Size: 1900 80 470 360 427
Fragments arranged by size:
1900 470 427 360 80
BtgI C'CryG_G
Cuts at: 0 1169 3083 3237
Size: 1169 1914 154
Fragments arranged by size:
1914 1169 154
BtsI GCAGTG_nn'
Cuts at: 0 1136 1640 1936 3237
Size: 1136 504 296 1301
Fragments arranged by size:
1301 1136 504 296
ClaI AT'CG_AT
Cuts at: 0 2381 3237
Size: 2381 856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 35 70 3237
Size: 35 35 3167
Fragments arranged by size:
3167 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1416 2145 2179 2655 3237
Size: 1416 729 34 476 582
Fragments arranged by size:
1416 729 582 476 34
DrdI GACnn_nn'nnGTC
Cuts at: 0 1599 3237
Size: 1599 1638
EaeI y'GGCC_r
Cuts at: 0 1302 2028 2517 3237
Size: 1302 726 489 720
Fragments arranged by size:
1302 726 720 489
EarI CTCTTCn'nnn_
Cuts at: 0 284 359 1347 2750 3237
Size: 284 75 988 1403 487
Fragments arranged by size:
1403 988 487 284 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 301 1715 1776 2098 2255 2774 2803
Size: 229 72 1414 61 322 157 519 29
Cuts at: 2803 2986 3237
Size: 183 251
Fragments arranged by size:
1414 519 322 251 229 183 157 72
61 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1808 3237
Size: 1808 1429
EcoO109I rG'GnC_Cy
Cuts at: 0 211 1606 2297 3237
Size: 211 1395 691 940
Fragments arranged by size:
1395 940 691 211
EcoRI G'AATT_C
Cuts at: 0 995 2934 3237
Size: 995 1939 303
Fragments arranged by size:
1939 995 303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 436 2326 3057 3237
Size: 436 1890 731 180
Fragments arranged by size:
1890 731 436 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 157 189 689 721 1446 1478
Size: 1 32 124 32 500 32 725 32
Cuts at: 1478 2315 2347 3237
Size: 837 32 890
Fragments arranged by size:
890 837 725 500 124 32 32 32
32 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 1256 1370 1995 3237
Size: 498 758 114 625 1242
Fragments arranged by size:
1242 758 625 498 114
MscI TGG'CCA
Cuts at: 0 1304 2030 3237
Size: 1304 726 1207
Fragments arranged by size:
1304 1207 726
MslI CAynn'nnrTG
Cuts at: 0 1078 3237
Size: 1078 2159
MspA1I CmG'CkG
Cuts at: 0 722 1710 2038 3006 3214 3237
Size: 722 988 328 968 208 23
Fragments arranged by size:
988 968 722 328 208 23
NcoI C'CATG_G
Cuts at: 0 1169 3237
Size: 1169 2068
NspI r_CATG'y
Cuts at: 0 511 574 1077 1324 1353 1602 3237
Size: 511 63 503 247 29 249 1635
Fragments arranged by size:
1635 511 503 249 247 63 29
PasI CC'CwG_GG
Cuts at: 0 1050 1918 3237
Size: 1050 868 1319
Fragments arranged by size:
1319 1050 868
PciI A'CATG_T
Cuts at: 0 570 1073 3237
Size: 570 503 2164
Fragments arranged by size:
2164 570 503
PflMI CCAn_nnn'nTGG
Cuts at: 0 1375 3237
Size: 1375 1862
PfoI T'CCnGG_A
Cuts at: 0 2915 3164 3237
Size: 2915 249 73
Fragments arranged by size:
2915 249 73
PmlI CAC'GTG
Cuts at: 0 2211 3237
Size: 2211 1026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1324 1356 3237
Size: 1324 32 1881
Fragments arranged by size:
1881 1324 32
PpuMI rG'GwC_Cy
Cuts at: 0 1606 2297 3237
Size: 1606 691 940
Fragments arranged by size:
1606 940 691
PshAI GACnn'nnGTC
Cuts at: 0 20 2159 2924 3237
Size: 20 2139 765 313
Fragments arranged by size:
2139 765 313 20
PspOMI G'GGCC_C
Cuts at: 0 211 3237
Size: 211 3026
PspXI vC'TCGA_Gb
Cuts at: 0 265 3237
Size: 265 2972
PstI C_TGCA'G
Cuts at: 0 126 491 1668 2667 3237
Size: 126 365 1177 999 570
Fragments arranged by size:
1177 999 570 365 126
PvuII CAG'CTG
Cuts at: 0 1710 2038 3006 3214 3237
Size: 1710 328 968 208 23
Fragments arranged by size:
1710 968 328 208 23
SfcI C'TryA_G
Cuts at: 0 122 487 494 1664 2663 3237
Size: 122 365 7 1170 999 574
Fragments arranged by size:
1170 999 574 365 122 7
SmlI C'TyrA_G
Cuts at: 0 265 1926 2244 3237
Size: 265 1661 318 993
Fragments arranged by size:
1661 993 318 265
StuI AGG'CCT
Cuts at: 0 2076 3237
Size: 2076 1161
StyI C'CwwG_G
Cuts at: 0 1169 1557 2570 2577 2697 3237
Size: 1169 388 1013 7 120 540
Fragments arranged by size:
1169 1013 540 388 120 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 1395 1474 2603 3237
Size: 535 860 79 1129 634
Fragments arranged by size:
1129 860 634 535 79
TatI w'GTAC_w
Cuts at: 0 549 3237
Size: 549 2688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1436 1468 1632 1664 2884 2916 3237
Size: 1436 32 164 32 1220 32 321
Fragments arranged by size:
1436 1220 321 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1610 3237
Size: 1610 1627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1258 1372 1417 1564 1654 3237
Size: 1258 114 45 147 90 1583
Fragments arranged by size:
1583 1258 147 114 90 45
XhoI C'TCGA_G
Cuts at: 0 265 3237
Size: 265 2972
XmnI GAAnn'nnTTC
Cuts at: 0 1341 2676 3237
Size: 1341 1335 561
Fragments arranged by size:
1341 1335 561
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AlfI AloI AlwNI
ApaI ApoI AseI AvaI BaeI BanI BanII BbsI
BbvCI BciVI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI
ClaI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AleI ApaLI
AscI AsiSI AvrII BamHI BbeI BcgI BclI BfrBI
BglI BmtI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI
BsrBI BssHII BstBI BstEII BstXI BstZ17I BtgZI DraI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PsiI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XmaI ZraI