Restriction map for
pF1KSDA0239
(Linear) (Six-base) MAPSORT of: KIAA0239.seq Check: 7405 from: 1 to: 2370
KIAA0239.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 258 967 1523 2370
Size: 258 709 556 847
Fragments arranged by size:
847 709 556 258
AatII G_ACGT'C
Cuts at: 0 1382 2370
Size: 1382 988
AccI GT'mk_AC
Cuts at: 0 1271 2370
Size: 1271 1099
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 1417 2180 2370
Size: 1318 99 763 190
Fragments arranged by size:
1318 763 190 99
AflII C'TTAA_G
Cuts at: 0 460 2370
Size: 460 1910
AflIII A'CryG_T
Cuts at: 0 608 733 2370
Size: 608 125 1637
Fragments arranged by size:
1637 608 125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 102 290 379 1813 2370
Size: 102 188 89 1434 557
Fragments arranged by size:
1434 557 188 102 89
AlwNI CAG_nnn'CTG
Cuts at: 0 917 938 1188 1326 1542 2062 2370
Size: 917 21 250 138 216 520 308
Fragments arranged by size:
917 520 308 250 216 138 21
ApaI G_GGCC'C
Cuts at: 0 1105 2370
Size: 1105 1265
AvaI C'yCGr_G
Cuts at: 0 351 1781 2321 2370
Size: 351 1430 540 49
Fragments arranged by size:
1430 540 351 49
BamHI G'GATC_C
Cuts at: 0 311 695 2370
Size: 311 384 1675
Fragments arranged by size:
1675 384 311
BanI G'GyrC_C
Cuts at: 0 270 705 955 1579 1664 2164 2370
Size: 270 435 250 624 85 500 206
Fragments arranged by size:
624 500 435 270 250 206 85
BanII G_rGCy'C
Cuts at: 0 302 379 446 884 924 1105 1118 1130
Size: 302 77 67 438 40 181 13 12
Cuts at: 1130 2370
Size: 1240
Fragments arranged by size:
1240 438 302 181 77 67 40 13
12
BbsI GAAGACnn'nnnn_
Cuts at: 0 642 1885 2370
Size: 642 1243 485
Fragments arranged by size:
1243 642 485
BbvCI CC'TCA_GC
Cuts at: 0 1232 1259 2370
Size: 1232 27 1111
Fragments arranged by size:
1232 1111 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 443 1660 1694 2370
Size: 409 34 1217 34 676
Fragments arranged by size:
1217 676 409 34 34
BclI T'GATC_A
Cuts at: 0 170 2370
Size: 170 2200
BglI GCCn_nnn'nGGC
Cuts at: 0 390 1148 2370
Size: 390 758 1222
Fragments arranged by size:
1222 758 390
BglII A'GATC_T
Cuts at: 0 893 2370
Size: 893 1477
BlpI GC'TnA_GC
Cuts at: 0 205 1241 1729 2370
Size: 205 1036 488 641
Fragments arranged by size:
1036 641 488 205
Bme1580I G_kGCm'C
Cuts at: 0 710 741 837 1105 1667 2370
Size: 710 31 96 268 562 703
Fragments arranged by size:
710 703 562 268 96 31
BmgBI CAC'GTC
Cuts at: 0 609 734 1945 2370
Size: 609 125 1211 425
Fragments arranged by size:
1211 609 425 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2070 2370
Size: 2070 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 532 541 1480 1667 1844 2370
Size: 460 72 9 939 187 177 526
Fragments arranged by size:
939 526 460 187 177 72 9
Bpu10I CC'TnA_GC
Cuts at: 0 1232 1259 2105 2370
Size: 1232 27 846 265
Fragments arranged by size:
1232 846 265 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 2370
Size: 685 1685
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 2029 2370
Size: 517 1512 341
Fragments arranged by size:
1512 517 341
BsaAI yAC'GTr
Cuts at: 0 1108 2370
Size: 1108 1262
BsaHI Gr'CG_yC
Cuts at: 0 1379 2370
Size: 1379 991
BsaWI w'CCGG_w
Cuts at: 0 158 2370
Size: 158 2212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 2370
Size: 514 30 1826
Fragments arranged by size:
1826 514 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 794 797 1216 1732 2353 2370
Size: 529 265 3 419 516 621 17
Fragments arranged by size:
621 529 516 419 265 17 3
BseYI C'CCAG_C
Cuts at: 0 100 380 573 906 917 1138 1364 2179
Size: 100 280 193 333 11 221 226 815
Cuts at: 2179 2370
Size: 191
Fragments arranged by size:
815 333 280 226 221 193 191 100
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1633 2329 2370
Size: 286 1347 696 41
Fragments arranged by size:
1347 696 286 41
BsiHKAI G_wGCw'C
Cuts at: 0 446 1091 2370
Size: 446 645 1279
Fragments arranged by size:
1279 645 446
BsmI GAATG_Cn'
Cuts at: 0 952 2370
Size: 952 1418
BsmBI CGTCTCn'nnnn_
Cuts at: 0 553 2370
Size: 553 1817
Bsp1286I G_dGCh'C
Cuts at: 0 302 379 446 710 741 837 884 924
Size: 302 77 67 264 31 96 47 40
Cuts at: 924 1091 1105 1118 1130 1667 2370
Size: 167 14 13 12 537 703
Fragments arranged by size:
703 537 302 264 167 96 77 67
47 40 31 14 13 12
BspEI T'CCGG_A
Cuts at: 0 158 2370
Size: 158 2212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 258 967 1523 2370
Size: 258 709 556 847
Fragments arranged by size:
847 709 556 258
BsrBI CCG'CTC
Cuts at: 0 1245 2370
Size: 1245 1125
BsrFI r'CCGG_y
Cuts at: 0 1227 2117 2370
Size: 1227 890 253
Fragments arranged by size:
1227 890 253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 863 938 947 1188 2370
Size: 863 75 9 241 1182
Fragments arranged by size:
1182 863 241 75 9
BstEII G'GTnAC_C
Cuts at: 0 1167 1762 2145 2370
Size: 1167 595 383 225
Fragments arranged by size:
1167 595 383 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2086 2370
Size: 2086 284
BstYI r'GATC_y
Cuts at: 0 185 311 695 893 1539 2370
Size: 185 126 384 198 646 831
Fragments arranged by size:
831 646 384 198 185 126
Bsu36I CC'TnA_GG
Cuts at: 0 623 848 2370
Size: 623 225 1522
Fragments arranged by size:
1522 623 225
BtgI C'CryG_G
Cuts at: 0 238 302 710 1020 1130 2370
Size: 238 64 408 310 110 1240
Fragments arranged by size:
1240 408 310 238 110 64
BtsI GCAGTG_nn'
Cuts at: 0 39 1723 1908 2370
Size: 39 1684 185 462
Fragments arranged by size:
1684 462 185 39
ClaI AT'CG_AT
Cuts at: 0 1574 2370
Size: 1574 796
DrdI GACnn_nn'nnGTC
Cuts at: 0 2074 2370
Size: 2074 296
EaeI y'GGCC_r
Cuts at: 0 391 545 1634 1648 2125 2370
Size: 391 154 1089 14 477 245
Fragments arranged by size:
1089 477 391 245 154 14
EarI CTCTTCn'nnn_
Cuts at: 0 4 1294 1408 2370
Size: 4 1290 114 962
Fragments arranged by size:
1290 962 114 4
EcoICRI GAG'CTC
Cuts at: 0 444 2370
Size: 444 1926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 532 541 1318 1417 1480 1667 1844
Size: 460 72 9 777 99 63 187 177
Cuts at: 1844 2180 2370
Size: 336 190
Fragments arranged by size:
777 460 336 190 187 177 99 72
63 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 940 1162 1258 1682 2370
Size: 940 222 96 424 688
Fragments arranged by size:
940 688 424 222 96
EcoO109I rG'GnC_Cy
Cuts at: 0 331 1101 1155 1422 1674 1743 2317 2370
Size: 331 770 54 267 252 69 574 53
Fragments arranged by size:
770 574 331 267 252 69 54 53
FspI TGC'GCA
Cuts at: 0 1177 2370
Size: 1177 1193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1941 1973 2370
Size: 1105 32 804 32 397
Fragments arranged by size:
1105 804 397 32 32
HincII GTy'rAC
Cuts at: 0 1272 2370
Size: 1272 1098
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1927 2370
Size: 1927 443
MscI TGG'CCA
Cuts at: 0 393 547 1636 1650 2370
Size: 393 154 1089 14 720
Fragments arranged by size:
1089 720 393 154 14
MslI CAynn'nnrTG
Cuts at: 0 84 178 1636 2370
Size: 84 94 1458 734
Fragments arranged by size:
1458 734 94 84
MspA1I CmG'CkG
Cuts at: 0 719 830 917 1185 1326 1595 2200 2313
Size: 719 111 87 268 141 269 605 113
Cuts at: 2313 2370
Size: 57
Fragments arranged by size:
719 605 269 268 141 113 111 87
57
NaeI GCC'GGC
Cuts at: 0 1229 2119 2370
Size: 1229 890 251
Fragments arranged by size:
1229 890 251
NcoI C'CATG_G
Cuts at: 0 238 2370
Size: 238 2132
NgoMIV G'CCGG_C
Cuts at: 0 1227 2117 2370
Size: 1227 890 253
Fragments arranged by size:
1227 890 253
NspI r_CATG'y
Cuts at: 0 689 826 1010 2370
Size: 689 137 184 1360
Fragments arranged by size:
1360 689 184 137
PasI CC'CwG_GG
Cuts at: 0 741 2370
Size: 741 1629
PflMI CCAn_nnn'nTGG
Cuts at: 0 543 917 1026 2370
Size: 543 374 109 1344
Fragments arranged by size:
1344 543 374 109
PfoI T'CCnGG_A
Cuts at: 0 188 1496 2370
Size: 188 1308 874
Fragments arranged by size:
1308 874 188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 2370
Size: 1051 32 1287
Fragments arranged by size:
1287 1051 32
PpuMI rG'GwC_Cy
Cuts at: 0 1155 1422 1674 1743 2317 2370
Size: 1155 267 252 69 574 53
Fragments arranged by size:
1155 574 267 252 69 53
PshAI GACnn'nnGTC
Cuts at: 0 611 2370
Size: 611 1759
PspOMI G'GGCC_C
Cuts at: 0 1101 2370
Size: 1101 1269
PstI C_TGCA'G
Cuts at: 0 934 1193 1520 1712 1980 2097 2370
Size: 934 259 327 192 268 117 273
Fragments arranged by size:
934 327 273 268 259 192 117
PvuII CAG'CTG
Cuts at: 0 719 830 1595 2200 2370
Size: 719 111 765 605 170
Fragments arranged by size:
765 719 605 170 111
SacI G_AGCT'C
Cuts at: 0 446 2370
Size: 446 1924
SalI G'TCGA_C
Cuts at: 0 1270 2370
Size: 1270 1100
SanDI GG'GwC_CC
Cuts at: 0 1743 2317 2370
Size: 1743 574 53
Fragments arranged by size:
1743 574 53
SapI GCTCTTCn'nnn_
Cuts at: 0 1408 2370
Size: 1408 962
ScaI AGT'ACT
Cuts at: 0 1288 2370
Size: 1288 1082
SexAI A'CCwGG_T
Cuts at: 0 1758 2370
Size: 1758 612
SfcI C'TryA_G
Cuts at: 0 930 1189 1516 1708 1976 2093 2370
Size: 930 259 327 192 268 117 277
Fragments arranged by size:
930 327 277 268 259 192 117
SmaI CCC'GGG
Cuts at: 0 2323 2370
Size: 2323 47
SmlI C'TyrA_G
Cuts at: 0 460 700 2370
Size: 460 240 1670
Fragments arranged by size:
1670 460 240
SphI G_CATG'C
Cuts at: 0 689 2370
Size: 689 1681
StuI AGG'CCT
Cuts at: 0 1553 2172 2370
Size: 1553 619 198
Fragments arranged by size:
1553 619 198
StyI C'CwwG_G
Cuts at: 0 238 2216 2370
Size: 238 1978 154
Fragments arranged by size:
1978 238 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 733 807 1357 1423 2370
Size: 733 74 550 66 947
Fragments arranged by size:
947 733 550 74 66
TatI w'GTAC_w
Cuts at: 0 1286 2370
Size: 1286 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 1951 1983 2370
Size: 514 32 1405 32 387
Fragments arranged by size:
1405 514 387 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 914 2370
Size: 914 1456
XmaI C'CCGG_G
Cuts at: 0 2321 2370
Size: 2321 49
XmnI GAAnn'nnTTC
Cuts at: 0 1402 2370
Size: 1402 968
ZraI GAC'GTC
Cuts at: 0 1380 2370
Size: 1380 990
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpiI
PpuMI PshAI PspOMI PstI PvuII SacI SalI SanDI
SapI ScaI SexAI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BfrBI BmrI BmtI BsaBI BsiEI BsiWI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PsiI PspXI PsrI PvuI RsrII SacII SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI