Restriction map for
pF1KA0228
(Linear) (Six-base) MAPSORT of: KIAA0228.seq Check: 9131 from: 1 to: 1755
KIAA0228.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 424 1127 1364 1721 1755
Size: 266 158 703 237 357 34
Fragments arranged by size:
703 357 266 237 158 34
AflII C'TTAA_G
Cuts at: 0 744 1755
Size: 744 1011
AgeI A'CCGG_T
Cuts at: 0 1631 1755
Size: 1631 124
AlwNI CAG_nnn'CTG
Cuts at: 0 337 1203 1755
Size: 337 866 552
Fragments arranged by size:
866 552 337
ApaLI G'TGCA_C
Cuts at: 0 638 1755
Size: 638 1117
ApoI r'AATT_y
Cuts at: 0 175 781 1045 1267 1330 1755
Size: 175 606 264 222 63 425
Fragments arranged by size:
606 425 264 222 175 63
AvaI C'yCGr_G
Cuts at: 0 106 1755
Size: 106 1649
BamHI G'GATC_C
Cuts at: 0 23 841 1755
Size: 23 818 914
Fragments arranged by size:
914 818 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 1226 1314 1755
Size: 1226 88 441
Fragments arranged by size:
1226 441 88
Bme1580I G_kGCm'C
Cuts at: 0 642 1755
Size: 642 1113
BmrI ACTGGGnnnn_n'
Cuts at: 0 175 1686 1755
Size: 175 1511 69
Fragments arranged by size:
1511 175 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 1561 1755
Size: 1561 194
BsaAI yAC'GTr
Cuts at: 0 984 1755
Size: 984 771
BsaWI w'CCGG_w
Cuts at: 0 1631 1755
Size: 1631 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 82 94 112 1755
Size: 64 18 12 18 1643
Fragments arranged by size:
1643 64 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1174 1755
Size: 1174 581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 602 1732 1755
Size: 503 99 1130 23
Fragments arranged by size:
1130 503 99 23
BsiEI CG_ry'CG
Cuts at: 0 8 1755
Size: 8 1747
BsiHKAI G_wGCw'C
Cuts at: 0 316 642 1755
Size: 316 326 1113
Fragments arranged by size:
1113 326 316
Bsp1286I G_dGCh'C
Cuts at: 0 316 642 1755
Size: 316 326 1113
Fragments arranged by size:
1113 326 316
BspHI T'CATG_A
Cuts at: 0 996 1218 1755
Size: 996 222 537
Fragments arranged by size:
996 537 222
BsrBI CCG'CTC
Cuts at: 0 80 98 1755
Size: 80 18 1657
Fragments arranged by size:
1657 80 18
BsrDI GCAATG_nn'
Cuts at: 0 701 1755
Size: 701 1054
BsrFI r'CCGG_y
Cuts at: 0 1631 1755
Size: 1631 124
BsrGI T'GTAC_A
Cuts at: 0 1275 1755
Size: 1275 480
BstYI r'GATC_y
Cuts at: 0 23 841 1755
Size: 23 818 914
Fragments arranged by size:
914 818 23
BtgI C'CryG_G
Cuts at: 0 8 1755
Size: 8 1747
BtsI GCAGTG_nn'
Cuts at: 0 177 821 868 1755
Size: 177 644 47 887
Fragments arranged by size:
887 644 177 47
DraI TTT'AAA
Cuts at: 0 558 1755
Size: 558 1197
DrdI GACnn_nn'nnGTC
Cuts at: 0 158 1755
Size: 158 1597
EaeI y'GGCC_r
Cuts at: 0 5 597 1755
Size: 5 592 1158
Fragments arranged by size:
1158 592 5
EagI C'GGCC_G
Cuts at: 0 5 1755
Size: 5 1750
EarI CTCTTCn'nnn_
Cuts at: 0 1020 1755
Size: 1020 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 424 1127 1364 1721 1755
Size: 266 158 703 237 357 34
Fragments arranged by size:
703 357 266 237 158 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 770 1132 1399 1429 1755
Size: 770 362 267 30 326
Fragments arranged by size:
770 362 326 267 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 571 603 1650 1682 1755
Size: 82 32 457 32 1047 32 73
Fragments arranged by size:
1047 457 82 73 32 32 32
HincII GTy'rAC
Cuts at: 0 163 1755
Size: 163 1592
HindIII A'AGCT_T
Cuts at: 0 136 752 1256 1755
Size: 136 616 504 499
Fragments arranged by size:
616 504 499 136
MfeI C'AATT_G
Cuts at: 0 1169 1499 1755
Size: 1169 330 256
Fragments arranged by size:
1169 330 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1266 1755
Size: 1266 489
MscI TGG'CCA
Cuts at: 0 599 1755
Size: 599 1156
MslI CAynn'nnrTG
Cuts at: 0 542 665 678 1755
Size: 542 123 13 1077
Fragments arranged by size:
1077 542 123 13
MspA1I CmG'CkG
Cuts at: 0 58 1755
Size: 58 1697
NspI r_CATG'y
Cuts at: 0 814 1755
Size: 814 941
PacI TTA_AT'TAA
Cuts at: 0 806 1755
Size: 806 949
PstI C_TGCA'G
Cuts at: 0 342 1755
Size: 342 1413
ScaI AGT'ACT
Cuts at: 0 206 1755
Size: 206 1549
SfcI C'TryA_G
Cuts at: 0 338 1493 1527 1755
Size: 338 1155 34 228
Fragments arranged by size:
1155 338 228 34
SmlI C'TyrA_G
Cuts at: 0 744 1755
Size: 744 1011
SspI AAT'ATT
Cuts at: 0 859 1651 1755
Size: 859 792 104
Fragments arranged by size:
859 792 104
StyI C'CwwG_G
Cuts at: 0 1302 1755
Size: 1302 453
SwaI ATTT'AAAT
Cuts at: 0 558 1755
Size: 558 1197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 866 1755
Size: 532 334 889
Fragments arranged by size:
889 532 334
TatI w'GTAC_w
Cuts at: 0 204 448 1275 1755
Size: 204 244 827 480
Fragments arranged by size:
827 480 244 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 1755
Size: 4 32 1719
Fragments arranged by size:
1719 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1036 1704 1755
Size: 1036 668 51
Fragments arranged by size:
1036 668 51
XmnI GAAnn'nnTTC
Cuts at: 0 1382 1755
Size: 1382 373
Enzymes that do cut:
AcuI AflII AgeI AlwNI ApaLI ApoI AvaI BamHI
BbsI Bme1580I BmrI BsaI BsaAI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI
BsrGI BstYI BtgI BtsI DraI DrdI EaeI EagI
EarI Eco57MI FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NspI PacI PstI ScaI SfcI
SmlI SspI StyI SwaI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaBI BsaHI BseYI BsiWI BsmI BsmBI
BspEI BspMI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI Tth111I XbaI
XhoI XmaI ZraI