Restriction map for
pF1KA0226
(Linear) (Six-base) MAPSORT of: KIAA0226.seq Check: 842 from: 1 to: 2781
KIAA0226.orf
With 182 enzymes: SgfI *
October 20, 2015 16:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1232 2781
Size: 1232 1549
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1335 1489 1786 2151 2781
Size: 1335 154 297 365 630
Fragments arranged by size:
1335 630 365 297 154
AfeI AGC'GCT
Cuts at: 0 2747 2781
Size: 2747 34
AflIII A'CryG_T
Cuts at: 0 2327 2781
Size: 2327 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1146 2781
Size: 1146 1635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 2781
Size: 1240 32 1509
Fragments arranged by size:
1509 1240 32
AlwNI CAG_nnn'CTG
Cuts at: 0 286 333 438 661 910 1467 2703 2781
Size: 286 47 105 223 249 557 1236 78
Fragments arranged by size:
1236 557 286 249 223 105 78 47
ApaI G_GGCC'C
Cuts at: 0 1947 2459 2781
Size: 1947 512 322
Fragments arranged by size:
1947 512 322
ApaLI G'TGCA_C
Cuts at: 0 133 2781
Size: 133 2648
ApoI r'AATT_y
Cuts at: 0 271 1636 2781
Size: 271 1365 1145
Fragments arranged by size:
1365 1145 271
AvaI C'yCGr_G
Cuts at: 0 247 815 2781
Size: 247 568 1966
Fragments arranged by size:
1966 568 247
BanI G'GyrC_C
Cuts at: 0 163 1232 1490 2781
Size: 163 1069 258 1291
Fragments arranged by size:
1291 1069 258 163
BanII G_rGCy'C
Cuts at: 0 412 706 1077 1763 1947 2459 2474 2582
Size: 412 294 371 686 184 512 15 108
Cuts at: 2582 2738 2781
Size: 156 43
Fragments arranged by size:
686 512 412 371 294 184 156 108
43 15
BbeI G_GCGC'C
Cuts at: 0 1494 2781
Size: 1494 1287
BbsI GAAGACnn'nnnn_
Cuts at: 0 1269 1448 2781
Size: 1269 179 1333
Fragments arranged by size:
1333 1269 179
BbvCI CC'TCA_GC
Cuts at: 0 1742 2781
Size: 1742 1039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1674 1708 2781
Size: 1674 34 1073
Fragments arranged by size:
1674 1073 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1044 2781
Size: 1044 1737
BfrBI ATG'CAT
Cuts at: 0 2507 2781
Size: 2507 274
BglI GCCn_nnn'nGGC
Cuts at: 0 2131 2781
Size: 2131 650
BglII A'GATC_T
Cuts at: 0 553 2213 2781
Size: 553 1660 568
Fragments arranged by size:
1660 568 553
BlpI GC'TnA_GC
Cuts at: 0 485 1538 2468 2781
Size: 485 1053 930 313
Fragments arranged by size:
1053 930 485 313
Bme1580I G_kGCm'C
Cuts at: 0 137 533 611 1947 2259 2459 2781
Size: 137 396 78 1336 312 200 322
Fragments arranged by size:
1336 396 322 312 200 137 78
BmgBI CAC'GTC
Cuts at: 0 612 1736 2781
Size: 612 1124 1045
Fragments arranged by size:
1124 1045 612
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 643 1569 1698 2781
Size: 446 197 926 129 1083
Fragments arranged by size:
1083 926 446 197 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 870 1050 1061 1548 1828 2725 2781
Size: 690 180 180 11 487 280 897 56
Fragments arranged by size:
897 690 487 280 180 180 56 11
Bpu10I CC'TnA_GC
Cuts at: 0 1242 1742 2781
Size: 1242 500 1039
Fragments arranged by size:
1242 1039 500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1662 2781
Size: 578 1084 1119
Fragments arranged by size:
1119 1084 578
BsaAI yAC'GTr
Cuts at: 0 114 1588 2781
Size: 114 1474 1193
Fragments arranged by size:
1474 1193 114
BsaBI GATnn'nnATC
Cuts at: 0 2134 2781
Size: 2134 647
BsaHI Gr'CG_yC
Cuts at: 0 1491 2781
Size: 1491 1290
BsaWI w'CCGG_w
Cuts at: 0 83 1878 2042 2781
Size: 83 1795 164 739
Fragments arranged by size:
1795 739 164 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1024 1054 1795 1825 2781
Size: 1024 30 741 30 956
Fragments arranged by size:
1024 956 741 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 873 1435 1438 2743 2746 2781
Size: 873 562 3 1305 3 35
Fragments arranged by size:
1305 873 562 35 3 3
BseYI C'CCAG_C
Cuts at: 0 230 1198 2140 2451 2781
Size: 230 968 942 311 330
Fragments arranged by size:
968 942 330 311 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1711 2048 2260 2781
Size: 647 1064 337 212 521
Fragments arranged by size:
1064 647 521 337 212
BsiHKAI G_wGCw'C
Cuts at: 0 137 706 1077 1243 1544 2474 2582 2781
Size: 137 569 371 166 301 930 108 199
Fragments arranged by size:
930 569 371 301 199 166 137 108
BsmI GAATG_Cn'
Cuts at: 0 489 1347 2127 2781
Size: 489 858 780 654
Fragments arranged by size:
858 780 654 489
BsmBI CGTCTCn'nnnn_
Cuts at: 0 618 2781
Size: 618 2163
Bsp1286I G_dGCh'C
Cuts at: 0 137 412 533 611 706 1077 1243 1544
Size: 137 275 121 78 95 371 166 301
Cuts at: 1544 1763 1947 2259 2459 2474 2582 2738 2781
Size: 219 184 312 200 15 108 156 43
Fragments arranged by size:
371 312 301 275 219 200 184 166
156 137 121 108 95 78 43 15
BspEI T'CCGG_A
Cuts at: 0 2042 2781
Size: 2042 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 965 1523 2210 2781
Size: 965 558 687 571
Fragments arranged by size:
965 687 571 558
BsrBI CCG'CTC
Cuts at: 0 414 2664 2781
Size: 414 2250 117
Fragments arranged by size:
2250 414 117
BsrDI GCAATG_nn'
Cuts at: 0 858 1058 1118 1140 2781
Size: 858 200 60 22 1641
Fragments arranged by size:
1641 858 200 60 22
BsrGI T'GTAC_A
Cuts at: 0 1208 2781
Size: 1208 1573
BssSI C'ACGA_G
Cuts at: 0 136 403 2116 2781
Size: 136 267 1713 665
Fragments arranged by size:
1713 665 267 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 207 2513 2781
Size: 207 2306 268
Fragments arranged by size:
2306 268 207
BstEII G'GTnAC_C
Cuts at: 0 1557 2781
Size: 1557 1224
BstYI r'GATC_y
Cuts at: 0 553 1113 1457 1478 2213 2781
Size: 553 560 344 21 735 568
Fragments arranged by size:
735 568 560 553 344 21
Bsu36I CC'TnA_GG
Cuts at: 0 924 2781
Size: 924 1857
BtgI C'CryG_G
Cuts at: 0 1754 2003 2738 2781
Size: 1754 249 735 43
Fragments arranged by size:
1754 735 249 43
BtsI GCAGTG_nn'
Cuts at: 0 159 216 334 347 1188 1716 1864 2781
Size: 159 57 118 13 841 528 148 917
Fragments arranged by size:
917 841 528 159 148 118 57 13
ClaI AT'CG_AT
Cuts at: 0 377 2781
Size: 377 2404
DrdI GACnn_nn'nnGTC
Cuts at: 0 310 2781
Size: 310 2471
EaeI y'GGCC_r
Cuts at: 0 317 794 1751 2006 2132 2348 2690 2781
Size: 317 477 957 255 126 216 342 91
Fragments arranged by size:
957 477 342 317 255 216 126 91
EarI CTCTTCn'nnn_
Cuts at: 0 924 1430 2236 2597 2781
Size: 924 506 806 361 184
Fragments arranged by size:
924 806 506 361 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1471 2781
Size: 1471 1310
EcoICRI GAG'CTC
Cuts at: 0 704 1075 2472 2580 2781
Size: 704 371 1397 108 201
Fragments arranged by size:
1397 704 371 201 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 870 1050 1061 1335 1489 1548 1786
Size: 690 180 180 11 274 154 59 238
Cuts at: 1786 1828 2151 2725 2781
Size: 42 323 574 56
Fragments arranged by size:
690 574 323 274 238 180 180 154
59 56 42 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1333 1420 2395 2781
Size: 1333 87 975 386
Fragments arranged by size:
1333 975 386 87
EcoO109I rG'GnC_Cy
Cuts at: 0 503 781 1084 1156 1944 2199 2400 2455
Size: 503 278 303 72 788 255 201 55
Cuts at: 2455 2456 2781
Size: 1 325
Fragments arranged by size:
788 503 325 303 278 255 201 72
55 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 575 2181 2781
Size: 575 1606 600
Fragments arranged by size:
1606 600 575
FspI TGC'GCA
Cuts at: 0 2155 2781
Size: 2155 626
HaeII r_GCGC'y
Cuts at: 0 1494 2749 2781
Size: 1494 1255 32
Fragments arranged by size:
1494 1255 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 248 280 538 570 596 628 1268 1300
Size: 248 32 258 32 26 32 640 32
Cuts at: 1300 1600 1632 2024 2056 2495 2527 2781
Size: 300 32 392 32 439 32 254
Fragments arranged by size:
640 439 392 300 258 254 248 32
32 32 32 32 32 32 26
HindIII A'AGCT_T
Cuts at: 0 1548 2781
Size: 1548 1233
KasI G'GCGC_C
Cuts at: 0 1490 2781
Size: 1490 1291
KpnI G_GTAC'C
Cuts at: 0 1236 2781
Size: 1236 1545
MscI TGG'CCA
Cuts at: 0 796 1753 2008 2134 2350 2692 2781
Size: 796 957 255 126 216 342 89
Fragments arranged by size:
957 796 342 255 216 126 89
MslI CAynn'nnrTG
Cuts at: 0 130 319 2120 2781
Size: 130 189 1801 661
Fragments arranged by size:
1801 661 189 130
MspA1I CmG'CkG
Cuts at: 0 833 957 1319 2024 2310 2740 2781
Size: 833 124 362 705 286 430 41
Fragments arranged by size:
833 705 430 362 286 124 41
NarI GG'CG_CC
Cuts at: 0 1491 2781
Size: 1491 1290
NcoI C'CATG_G
Cuts at: 0 1754 2781
Size: 1754 1027
NsiI A_TGCA'T
Cuts at: 0 2509 2781
Size: 2509 272
NspI r_CATG'y
Cuts at: 0 1257 2331 2511 2781
Size: 1257 1074 180 270
Fragments arranged by size:
1257 1074 270 180
PciI A'CATG_T
Cuts at: 0 2327 2781
Size: 2327 454
PflMI CCAn_nnn'nTGG
Cuts at: 0 574 2781
Size: 574 2207
PfoI T'CCnGG_A
Cuts at: 0 1077 1460 1820 2768 2781
Size: 1077 383 360 948 13
Fragments arranged by size:
1077 948 383 360 13
PmlI CAC'GTG
Cuts at: 0 114 1588 2781
Size: 114 1474 1193
Fragments arranged by size:
1474 1193 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 884 1660 1692 2781
Size: 852 32 776 32 1089
Fragments arranged by size:
1089 852 776 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 503 2199 2400 2781
Size: 503 1696 201 381
Fragments arranged by size:
1696 503 381 201
PspOMI G'GGCC_C
Cuts at: 0 1943 2455 2781
Size: 1943 512 326
Fragments arranged by size:
1943 512 326
PstI C_TGCA'G
Cuts at: 0 200 212 329 2672 2781
Size: 200 12 117 2343 109
Fragments arranged by size:
2343 200 117 109 12
PvuII CAG'CTG
Cuts at: 0 833 957 1319 2310 2781
Size: 833 124 362 991 471
Fragments arranged by size:
991 833 471 362 124
SacI G_AGCT'C
Cuts at: 0 706 1077 2474 2582 2781
Size: 706 371 1397 108 199
Fragments arranged by size:
1397 706 371 199 108
SacII CC_GC'GG
Cuts at: 0 2741 2781
Size: 2741 40
ScaI AGT'ACT
Cuts at: 0 711 2098 2176 2781
Size: 711 1387 78 605
Fragments arranged by size:
1387 711 605 78
SfcI C'TryA_G
Cuts at: 0 196 208 325 2262 2668 2781
Size: 196 12 117 1937 406 113
Fragments arranged by size:
1937 406 196 117 113 12
SfoI GGC'GCC
Cuts at: 0 1492 2781
Size: 1492 1289
SmlI C'TyrA_G
Cuts at: 0 593 1677 2781
Size: 593 1084 1104
Fragments arranged by size:
1104 1084 593
SphI G_CATG'C
Cuts at: 0 2511 2781
Size: 2511 270
StyI C'CwwG_G
Cuts at: 0 1754 2195 2351 2781
Size: 1754 441 156 430
Fragments arranged by size:
1754 441 430 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1212 2781
Size: 1212 1569
TatI w'GTAC_w
Cuts at: 0 709 1208 2096 2174 2411 2781
Size: 709 499 888 78 237 370
Fragments arranged by size:
888 709 499 370 237 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 1795 1827 2781
Size: 1024 32 739 32 954
Fragments arranged by size:
1024 954 739 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2377 2781
Size: 2377 404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 940 2358 2700 2781
Size: 804 136 1418 342 81
Fragments arranged by size:
1418 804 342 136 81
XmnI GAAnn'nnTTC
Cuts at: 0 2329 2781
Size: 2329 452
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII
BstYI Bsu36I BtgI BtsI ClaI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HindIII KasI KpnI MscI MslI MspA1I NarI
NcoI NsiI NspI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII SacI SacII ScaI SfcI
SfoI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AscI AseI AsiSI AvrII BaeI BamHI BclI BmtI
BplI BsaI BsiEI BsiWI BspHI BsrFI BssHII BstBI
BstXI BstZ17I BtgZI CspCI DraI DraIII EagI EcoRI
EcoRV FseI FspAI HincII HpaI MfeI MluI MmeI
NaeI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI
XmaI ZraI